Meiotic role of SWI6 in Saccharomyces Cerevisiae

Araki, Hiroyuki,Leem, Sun-Hee,Chung, Chung-Nam,Sunwoo, Yangil 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6

The transcript levels of DNA replication genes and some recombination genes in Saccharomyces cerevisiae fluctuate and peak at the G1/S boundary in the mitotic cell cycle. This fluctuation is regulated by MCB(Mlu/cell cycle box) elements which are bound by the DSC1/MBF1 complex consisting of Swi6 and Mbp1. It is also known that some of the MCB-regulated genes are induced by treatment with DNA damaging agents and in meiosis. In this report, the function of △SWI6 in meiosis was investigated. △swi6 cells underwent sporulation as did wild-type cells. However, the deletion mutant cells showed reduced spore viability and lower frequency of recombination. The transcript levels of the recombination genes RAD51 and RAD54, which have MCB elements, were reduced in △swi6 cells. The transcript levels of SW16 itself were also induced and declined in meiosis. Furthermore, an increased dosage of SW16 enhanced the transcript level of the RAD51 gene and also the recombination frequency in meiosis. These results suggest that SW16 enhances the expression level of the recombination genes in meiosis in a dosage-dependent manner, which results in an effect on the frequency of meiotic recombination.

RADIONUCLIDE STUDY ON HEPATIC BLOOD FLOW IN SCHISTO-SOMIASIS JAPONICA

Araki, Tsutomu,Uchiyama, Guio,Okada, Junichi,Hayakawa, Kazushige,Hayashi, Sanshin,Iuchi, Masahiko 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

Korean, Chinese and Foreign Foods in Japan

ARAKI Hitoshi 대한지리학회 2008 대한지리학회 컨퍼런스 자료집 Vol.2 No.-

Experiences in Sport, Physical Activity, and Physical Education Among Christian, Buddhist, and Hindu Asian Adolescent Girls

Araki, Kaori,Kodani, Iku,Gupta, Nidhi,Gill, Diane L. The Korean Society for Preventive Medicine 2013 예방의학회지 Vol.46 No.suppl

Multicultural scholarship in sport and exercise psychology should help us understand and apply cultural competencies for all to be physically active. In the present study, two Asian countries, Japan and Singapore, were chosen. The participation rate for physical activities among adolescent girls tends to be lower than that of boys in both countries. Thus, the purpose of the project was to gain knowledge and understanding about sociocultural factors that may explain adolescent girls' perceptions and behaviors toward sport, physical activity, and physical education (PE). A qualitative approach using semi-structured interviews with focus groups was used to understand meanings of physical activity among Buddhist Japanese, and Hindu Indians and Christian Chinese from Singapore. Each focus group consisted of four or five girls and female researchers. Based on the analysis, we created four themes which were "cultural identities", "Asian girls and sport/physical activities", "PE experiences", "motivation for future involvement". The Buddhist Japanese, Hindu Indian, and Christian Chinese participants each reported unique physical activity experiences, and all the participants were aware of how Asian culture may affect being physically active. Experiences of PE classes were similar but perceptions of their PE attire were different for Christian Chinese and Hindu Indian adolescent girls. Based on the results, the importance of nurturing cultural competencies and ways to encourage girls to be physically active throughout life were discussed.

Measurement of Gamma-ray Yield from Thick Carbon Target Irradiated by 5 and 9 MeV Deuterons

Araki, Shouhei,Kondo, Kazuhiro,Kin, Tadahiro,Watanabe, Yukinobu,Shigyo, Nobuhiro,Sagara, Kenshi The Korean Association for Radiation Protection 2017 방사선방어학회지 Vol.42 No.1

Background: The design of deuteron accelerator neutron source facilities requires reliable yield estimation of gamma-rays as well as neutrons from deuteron-induced reactions. We have so foar measured systematically double-differential thick target neutron yields (DDTTNYs) for carbon, aluminum, titanium, copper, niobium, and SUS304 targets. In the neutron data analysis, the events of gamma-rays taken simultaneously were treated as backgrounds. In the present work, we have re-analyzed the experimental data for a thick carbon target with particular attention to gamma-ray events. Materials and Methods: Double-differential thick target gamma-ray yields from carbon irradiated by 5 and 9 MeV deuterons were measured using an NE213 liquid organic scintillator at the Kyushu University Tandem accelerator Laboratory. The gamma-ray energy spectra were obtained by an unfolding method using FORIST code. The response functions of the NE213 detector were calculated by EGS5 incorporated in PHITS code. Results and Discussion: The measured gamma-ray spectra show some pronounced peaks corresponding to gamma-ray transitions between discrete levels in residual nuclei, and the measured angular distributions are almost isotropic for both the incident energies. Conclusion: PHITS calculations using INCL, GEM, and EBITEM models reproduce the spectral shapes and the angular distributions generally well, although they underestimate the absolute gamma-ray yields by about 20%.

Fabrication of Nanoshell-Based 3D Periodic Structures by Templating Process using Solution-derived ZnO

Araki, Shinji,Ishikawa, Yasuaki,Wang, Xudongfang,Uenuma, Mutsunori,Cho, Donghwi,Jeon, Seokwoo,Uraoka, Yukiharu Springer US 2017 NANOSCALE RESEARCH LETTERS Vol.12 No.1

<P>Fabrication methods for a 3D periodic nanostructure with excellent and unique properties for various applications, such as photonic and phononic crystals, have attracted considerable interest. Templating processes using colloidal crystals have been proposed to create nanoshell-based 3D structures over a large area with ease. However, there are technical limitations in structural design, resulting in difficulties for structural flexibility. Here, we demonstrate a combination of proximity field nanopatterning and infiltration processes using solution-derived ZnO for a nanoshell-based 3D periodic structure with high structural flexibility and controllability. A unique process of infiltration of a solution-derived material into a polymeric template prepared by a proximity field nanopatterning process achieves the fabrication of a pre-formed layer that works as a protective layer for the template and framework for the inverse structure. Subsequently, this process shows the controllability of nanoshell thickness and significant improvement in the structure height shrinkage factor (16%) compared to those of a previous non-vacuum infiltration method (34%). The proposed method offers high controllability and flexibility in the design of structural sizes, leading to further development toward nanoshell-based 3D structures for various applications including energy devices and sensors.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s11671-017-2186-6) contains supplementary material, which is available to authorized users.</P>

An Over View of the Guhan System and its Administration in Japan

Araki Nobuyoshi 강원대학교 비교법학연구소 1996 江原法學 Vol.8 No.-

Cover Crop Use in Tomato Production in Plastic High Tunnel

Araki Hajime,Shiori Hane,Yoichiro Hoshino,Toshiyuki Hirata 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.4

More than 70% of fresh tomato is produced in plastic high tunnel or greenhouse and much fertilizer and agricultural chemicals have been applied for tomato production. Cover crop is one of the important tools for sustainable agro-production because of improving soil properties. Effect of cover cropping on soil properties and tomato production in plastic high tunnel was examined in Sapporo, Japan, snow cover region. Plastic high tunnel was set before snow-melting, late in March, 2007. Two cover crops, legume (hairy vetch; Vicia villosa R.) and non legume (wild oat; Avena sterigosa L.), were planted each alone or mix-planted in the plot (0.8 m in width and 3.5 m in length) early in April. Seeding density in mono-culture was 5 ㎏/10a in hairy vetch and 10 ㎏/10a in wild oat. For bi-culture, density was 3.5 ㎏/10a in hairy vetch and 5 ㎏/10a in wild oat. Above ground biomass (dry weight) was shown 445 ㎏/10a in hairy vetch and 982 ㎏/10a in wild oat late in May. However, in mix-culture, it was 190 ㎏/10a in hairy vetch and 602 ㎏/10a in wild oat. Cover crops were killed for residue mulch just before tomato planting. Seedlings of tomato ‘Momotaro’ were planted in the plots covered with cover crop residue and 12 ㎏/10a N fertilizer application (12 ㎏ N/10a) and bare plot with 12 ㎏/10a or 24 ㎏/10a N fertilizer (24 ㎏ N/10a) application. Nitrate concentration of the petiole sap in the leaf just below the first fruit cluster was higher in the tomatoes grown in bare plot with 24㎏ N/10a and in hairy vetch mulch with 12㎏ N/10a at 40 days after transplanting. Large yield was shown in bare plot with 24㎏ N/10a (7.9t/10a) and in hairy vetch plot with 12㎏ N/10a (7.8t/10a). The yield in bare plot with 12㎏ N/10a and Mix plot with 12㎏ N/10a was 6.9t/10a. The yield in wild oat plot with 12㎏ N/10a was the smallest (6.1t/10a). Soil carbon in the plots with cover crop mulch became higher than that of bare plots after tomato production. It will be possible to obtain current yield and to increase soil carbon in cover cropping with reduction of N fertilizer. This technique will lead the organically grown system in plastic high tunnel production.

Rapid measurements of hydrogen cyanide concentration in combustion gas via terahertz spectroscopy

Araki Mitsunori,Matsuyama Ken 한국물리학회 2022 Current Applied Physics Vol.36 No.-

The development of a real-time measurement system to determine the concentrations of combustion gases mixed with smoke particles in fire environments is an essential technical issue in the fire safety field. In this study, the absorption line for the J = 8–7 rotational transition at 708.9 GHz of hydrogen cyanide (HCN) produced by the combustion of silk is rapidly measured in a 1 m cell during repeat scans (seven times a minute) via terahertz spectroscopy. The obtained time profile of HCN concentrations agrees well with the time profile obtained via Fourier transform infrared (FT-IR) spectroscopy. The maximum concentrations derived via terahertz spectroscopy are consistent with those derived via FT-IR within an accuracy of 10%. The minimum sensitivity of HCN is 100 ppm as detected via terahertz spectroscopy. The repetition rate, the accuracy, and the sensitivity for the scans demonstrate the potential of terahertz spectroscopy to rapidly diagnose combustion gas flow concentrations in fire environments.

Peroxidase isozymes of the Lycoris radiata triploids obtained from different localities in Japan

ARAKI, Hiroto,HIDAKA, Saburo 韓國自然史博物學會 1986 生物과 自然 Vol.16 No.1

Starch gel electrophoresis showed that the peroxidase banding patterns in a triploid Lycoris radiata(2n=33) varied with time, but the changes were regular and followed the annual cycle of seasons. In addition, the peroxidase banding patterns revealed no phenotypic differences among thousands of plants from 24 localities, from Miyagi in the north to Kagoshima in the south. This suggests that all the individuals of L. radiata in Japan are identical in genic constitution and also suggests that the dispersal of L. radiata depends in all respects on man and his activities.