Green synthesis of carbon nanodots from agro-industrial residues

Allan Jr. Gonçalves Afonso,Felipe Thomaz Aquino,Gisele M. L. Dalmônico,Matheus V. Nascimento,Ernesto Wrasse,Kelen M. F. Rossi de Aguiar 한국탄소학회 2022 Carbon Letters Vol.32 No.1

Fluorescent nanostructures based on carbon, or carbon dots, are attracting much attention and interest because of their diverse properties which can be applied in several fields of knowledge, such as optics, biomedicine, environmental research, among others. Such properties are in part, derived from its intrinsic luminescence from tunable functional groups. In this work, we produced carbon nanodots (CND) using agro-industrial residues, such as Lolium perenne and malt bagasse. The methods used were conventional hydrothermal syntheses and microwave-assisted hydrothermal synthesis. To the best of our knowledge, this is the first time that carbon dots synthesized from this ryegrass type are reported. The synthesis methods were one step (no catalyst, base, or acid were added for passivation), and the functional groups responsible for the luminescence and high solubility in water were identified by infrared spectroscopy, being mainly C=O, C–OH, C–N, and N–H. According to our theoretical studies, the C=O group introduced a new energy level for electronic transitions that can affect the emission properties. Fluorescence images of osteoblasts using CNDs were acquired and their chelating property towards Pb2+ and Cr6+ detection was tested.

Measurement of <i>CP</i> asymmetries in <i>D</i> ^± → <i>η</i>′<i>π</i> ^± and D s ± → ^{η ′} ^{π ±} decays

Aaij, R.,Adeva, B.,Adinolfi, M.,Ajaltouni, Z.,Akar, S.,Albrecht, J.,Alessio, F.,Alexander, M.,Ali, S.,Alkhazov, G.,Alvarez Cartelle, P.,Alves Jr, A.A.,Amato Jr, S.,Amerio Jr, S.,Amhis Jr, Y.,An Jr, L. Elsevier 2017 Physics letters: B Vol.771 No.-

<P><B>Abstract</B></P> <P>A search for <I>CP</I> violation in <SUP> D ± </SUP> → <SUP> η ′ </SUP> <SUP> π ± </SUP> and D s ± → <SUP> η ′ </SUP> <SUP> π ± </SUP> decays is performed using proton–proton collision data, corresponding to an integrated luminosity of 3 <SUP> fb − 1 </SUP> , recorded by the LHCb experiment at centre-of-mass energies of 7 and 8 TeV. The measured <I>CP</I>-violating charge asymmetries are <SUB> A CP </SUB> ( <SUP> D ± </SUP> → <SUP> η ′ </SUP> <SUP> π ± </SUP> ) = ( − 0.61 ± 0.72 ± 0.53 ± 0.12 ) % and <SUB> A CP </SUB> ( D s ± → <SUP> η ′ </SUP> <SUP> π ± </SUP> ) = ( − 0.82 ± 0.36 ± 0.22 ± 0.27 ) % , where the first uncertainties are statistical, the second systematic, and the third are the uncertainties on the <SUB> A CP </SUB> ( <SUP> D ± </SUP> → K S 0 <SUP> π ± </SUP> ) and <SUB> A CP </SUB> ( D s ± → ϕ <SUP> π ± </SUP> ) measurements used for calibration. The results represent the most precise measurements of these asymmetries to date.</P>

A numerical-experimental evaluation of beams composed of a steel frame with welded and conventional stirrups

Wagner L. Gonçalves,Guilherme F. Gomes,Yohan D. Mendéz,Fabrício A. Almeida,Valquíria C. Santos,Sebastião S. Cunha Jr. 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.22 No.1

Reinforced concrete structures are widely used in civil engineering projects around the world in different designs. Due to the great evolution in computational equipment and numerical methods, structural analysis has become more and more reliable, and in turn more closely approximates reality. Thus among the many numerical methods used to carry out these types of analyses, the finite element method has been highlighted as an optimized tool option, combined with the non-linear and linear analysis techniques of structures. In this paper, the behavior of reinforced concrete beams was analyzed in two different configurations: i) with welding and ii) conventionally lashed stirrups using annealed wire. The structures were subjected to normal and tangential forces up to the limit of their bending resistance capacities to observe the cracking process and growth of the concrete structure. This study was undertaken to evaluate the effectiveness of welded wire fabric as shear reinforcement in concrete prismatic beams under static loading conditions. Experimental analysis was carried out in order compare the maximum load of both configurations, the experimental load-time profile applied in the first configuration was used to reproduce the same loading conditions in the numerical simulations. Thus, comparisons between the numerical and experimental results of the welded frame beam show that the proposed model can estimate the concrete strength and failure behavior accurately.

Morphine Spinal Block Anesthesia in Patients Who Undergo an Open Hemorrhoidectomy: A Prospective Analysis of Pain Control and Postoperative Complications

Hélio Moreira Jr,José PT Moreira,Raniere R Isaac,Onofre Alves-Neto,Thiago AC Moreira,Tiago HM Vieira,Andressa MS Brasil 대한대장항문학회 2014 Annals of Coloproctolgy Vol.30 No.3

Purpose: This study evaluated the use of adding morphine to bupivacaine in spinal anesthesia for pain control in patients who underwent an open hemorrhoidectomy. Methods: Forty patients were prospectively selected for an open hemorrhoidectomy at the same institution and were randomized into two groups of 20 patients each: group 1 had a spinal with 7 mg of heavy bupivacaine associated with 80 μg of morphine (0.2 mg/mL). Group 2 had a spinal with 7 mg of heavy bupivacaine associated with distilled water, achieving the same volume of spinal infusion as that of group 1. Both groups were prescribed the same pain control medicine during the postoperative period. Pain scores were evaluated at the anesthetic recovery room and at 3, 6, 12, and 24 hours after surgery. Postoperative complications, including pruritus, nausea, headaches, and urinary retention, were also recorded. Results: There were no anthropometric statistical differences between the two groups. Pain in the anesthetic recovery room and 3 hours after surgery was similar for both groups. However, pain was better controlled in group 1 at 6 and 12 hours after surgery. Although pain was better controlled for group 1 after 24 hours of surgery, the difference between the groups didn’t achieved statistical significance. Complications were more common in group 1. Six patients (6/20) presented coetaneous pruritus and 3 with (3/20) urinary retention. Conclusion: A hemorrhoidectomy under a spinal with morphine provides better pain control between 6 and 12 hours after surgery. However, postoperative complications, including cutaneous pruritus (30%) and urinary retention (15%), should be considered as a negative side of this procedure.

Impedance-based health monitoring and mechanical testing of structures

Lizeth Vargas Palomino,Valder Steffen Jr.,Jose dos Reis Vieira de Moura Jr.,Karina Mayumi Tsuruta,Domingos Alves Rade 국제구조공학회 2011 Smart Structures and Systems, An International Jou Vol.7 No.1

The mechanical properties obtained from mechanical tests, such as tensile, buckling, impact and fatigue tests, are largely applied to several materials and are used today for preliminary studies for the investigation of a desired element in a structure and prediction of its behavior in use. This contribution focus on two widely used different tests: tensile and fatigue tests. Small PZT (Lead Titanate Zirconate) patches are bonded on the surface of test samples for impedance-based health monitoring purposes. Together with these two tests, the electromechanical impedance technique was performed by using aluminum test samples similar to those used in the aeronautical industry. The results obtained both from tensile and fatigue tests were compared with the impedance signatures. Finally, statistical meta-models were built to investigate the possibility of determining the state of the structure from the impedance signatures.

Impedance-based health monitoring and mechanical testing of structures

Palomino, Lizeth Vargas,de Moura, Jose Dos Reis Vieira Jr.,Tsuruta, Karina Mayumi,Rade, Domingos Alves,Steffen, Valder Jr. Techno-Press 2011 Smart Structures and Systems, An International Jou Vol.7 No.1

The mechanical properties obtained from mechanical tests, such as tensile, buckling, impact and fatigue tests, are largely applied to several materials and are used today for preliminary studies for the investigation of a desired element in a structure and prediction of its behavior in use. This contribution focus on two widely used different tests: tensile and fatigue tests. Small PZT (Lead Titanate Zirconate) patches are bonded on the surface of test samples for impedance-based health monitoring purposes. Together with these two tests, the electromechanical impedance technique was performed by using aluminum test samples similar to those used in the aeronautical industry. The results obtained both from tensile and fatigue tests were compared with the impedance signatures. Finally, statistical meta-models were built to investigate the possibility of determining the state of the structure from the impedance signatures.

Prevention of Prostate Cancer in Transgenic Adenocarcinoma of the Mouse Prostate Mice by Yellow Passion Fruit Extract and Antiproliferative Effects of Its Bioactive Compound Piceatannol

Larissa Akemi Kido,Eun-Ryeong Hahm,Su-Hyeong Kim,Andressa Mara Baseggio,Valeria Helena Alves Cagnon,Shivendra V. Singh,Mário Roberto Maróstica Jr 대한암예방학회 2020 Journal of cancer prevention Vol.25 No.2

Piceatannol (PIC), a polyphenol presents in many vegetables and fruits including yellow passion fruit extract (PFE; Passiflora edulis), has anti-cancer activity, but its molecular targets are still poorly understood. The aims of this study were to investigate the molecular mechanistic actions of PIC in prostate cancer cell lines and to test if the extract from PFE rich in PIC can affect the growth of prostate cancer cells in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model. The PC-3, 22Rv1, LNCaP, and VCaP prostate cancer cells were exposed to PIC (10-40 μM), and cell viability, lactate measurement, Western blot, and flow cytometric analyses were performed. For an in vivo experiments, eight-week-old TRAMP mice (n = 10 per group each) received an aqueous extract of PFE containing 20 mg of PIC/kg or water (control group) by gavage for 4 or 10 weeks for further analyses. PIC treatment concentration- and time-dependently reduced viability of all cell lines tested. 22Rv1 and LNCaP cells treated with PIC did not exhibit any significant alteration in the intracellular accumulation of lactate. PIC treatment caused G0/G1 phase cell cycle arrest and induction of apoptosis in both LNCaP and 22Rv1 cells. PIC-treated cells exhibited altered protein levels of p53, p21, cyclin D1, and cyclin-dependent kinase 4 (cdk4). The short and long-term PFE treatments also affected p21, cyclin D1 and cdk4 and delayed disease progression in TRAMP, with a decreased incidence of preneoplastic lesions. In conclusion, PIC apparently does not alter glucose metabolism in prostate cancer cells, while cell cycle arrest and p53 modulation are likely important in anti-cancer effects of PIC alone or as a food matrix byproduct in prostate cancer cells, especially those with an androgen-dependent phenotype.

Brazilian Berry Extract Differentially Induces Inflammatory and Immune Responses in Androgen Dependent and Independent Prostate Cancer Cells

Larissa Akemi Kido,Isabela Maria Urra Rossetto,Andressa Mara Baseggio,Gabriela Bortolanza Chiarotto,Letícia Ferreira Alves,Felipe Rabelo Santos,Celina de Almeida Lamas,Mário Roberto Maróstica Jr,Valér 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.3

Jaboticaba is a Brazilian berry, which is rich in fibers and bioactive compounds and shows high antioxidant and antiproliferative activities. Prostate cancer (PCa) is the second most common type of cancer among men and its progression is influenced by androgens and inflammation. Previous studies reported the ability of the jaboticaba to modulate pathways involved in prostate diseases. The main objective of this study was to provide significant data about molecular targets of the jaboticaba peel extract (JPE) and its mechanisms of action in PCa cell lines with different androgenic status (LNCaP and PC-3). The results showed that JPE was able to decrease cell viability in both cell lines. LNCaP showed more sensitivity to JPE exposure, indicating the efficacy of the JPE treatment in terms of androgen responsiveness. JPE showed a distinct hormone dependent effect on the NF-κB signaling, with reduced NF-κB levels for LNCaP and increased NF-κB levels in PC-3 cells. Mechanisms related to cell death by apoptosis were stimulated after the JPE treatment, modulating B-cell lymphoma 2 and BAX for LNCaP and PC-3. Particularly for PC-3, the JPE treatment resulted in cytokine-cytokine receptor interaction activation mostly by up regulating pro-inflammatory, pro-angiogenic, immunostimulatory and immunosuppressive genes. Also, a set of genes related to angiogenesis and metastasis were down-regulated by JPE. In conclusion, JPE exerted an antitumor effect on PCa for both cell lines which can be enhanced if androgenic reliance is considered.

Purification, and Biochemical and Biophysical Characterization of Cellobiohydrolase I from Trichoderma harzianum IOC 3844

( Colussi Francieli ),( Viviane Serpa ),( Priscila Da Silva Delabona ),( Livia Regina Manzine ),( Maria Luiza Voltatodio ),( Renata Alves ),( Bruno Luan Mello ),( Nei Pereira Jr. ),( Cristiane Sanches 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.8

Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatographic method for purification of ThCBHI and its initial biophysical and biochemical characterization. The ThCBHI produced by induction with microcrystalline cellulose under submerged fermentation was purified on DEAE-Sephadex A-50 media and its identity was confirmed by mass spectrometry. The ThCBHI biochemical characterization showed that the protein has a molecular mass of 66 kDa and pI of 5.23. As confirmed by smallangle X-ray scattering (SAXS), both full-length ThCBHI and its catalytic core domain (CCD) obtained by digestion with papain are monomeric in solution. Secondary structure analysis of ThCBHI by circular dichroism revealed α- helices and β-strands contents in the 28% and 38% range, respectively. The intrinsic fluorescence emission maximum of 337 nm was accounted for as different degrees of exposure of ThCBHI tryptophan residues to water. Moreover, ThCBHI displayed maximum activity at pH 5.0 and temperature of 50℃ with specific activities against Avicel and p-nitrophenyl-β-D-cellobioside of 1.25 U/mg and 1.53 U/mg, respectively.