Essential oils as growth-promoting additives on performance, nutrient digestibility, cecal microbes, and serum metabolites of broiler chickens: a meta-analysis

Irawan, Agung,Hidayat, Cecep,Jayanegara, Anuraga,Ratriyanto, Adi Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

Objective: The purpose of this meta-analysis was to evaluate the effect of dietary essential oils (EOs) on productive performance, nutrient digestibility, and serum metabolite profiles of broiler chickens and to compare their effectiveness as growth-promoting additives against antibiotics. Methods: Peer-reviewed articles were retrieved from Web of Science, Science Direct, PubMed, and Google scholar and selected based on pre-determined criteria. A total of 41 articles containing 55 experiments with 163 treatment units were eligible for analyses. Data were subjected to a meta-analysis based on mixed model methodology considering the doses of EOs as fixed effects and the different studies as random effects. Results: Results showed a linear increase (p<0.001) on body weight gain (BWG) where Antibiotics (FCR) and average daily feed intake decreased (p<0.001) linearly with an increasing dose of EOs. Positive effects were observed on the increased (p<0.01) digestibility of dry matter, crude protein, ether extract, and cecal Lactobacillus while Escherichia coli (E. coli) population in the cecum decreased (p<0.001) linearly. There was a quadratic effect on the weight of gizzard (p<0.01), spleen (p<0.05), bursa of fabricius (p<0.001), and liver (p<0.10) while carcass, abdominal fat, and pancreas increased (p<0.01) linearly. The dose of EOs linearly increased high density lipoprotein, glucose, protein, and globulin concentrations (p<0.01). In comparison to control and antibiotics, all type of EOs significantly reduced (p<0.001) FCR and tended to increase (p<0.1) BWG and final body weight. Cinnamaldehyde-compound was the only EOs type showing a tendency to increase (p<0.1) carcass weight, albumin, and protein of serum metabolites while this EOs together with EOs-Blend 1 decreased (p<0.01) E. coli population. Low density lipoprotein concentration decreased (p<0.05) with antibiotics and carvacrol-based compound when compared to the control group. Conclusion: This evidence confirms that EOs are suitable to be used as growth promoters and their economical benefit appears to be promising.

Effect of feeding fermented soybean meal on broiler chickens’ performance: a meta-analysis

Irawan Agung,Ratriyanto Adi,Respati Adib Norma,Ningsih Niati,Fitriastuti Rahma,Suprayogi Wara Pratitis Sabar,Hadi Rendi Fathoni,Setyono Wahyu,Akhirini Novi,Jayanegara Anuraga 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.12

Objective: The present study aimed to quantify the effects of fermented soybean meal (FSBM) on broiler chickens’ performance by employing a meta-analysis approach. Methods: A total of 16 studies were included in the database after being systematically selected using a PRISMA protocol. Hedges’ g effect size was used to quantify pooled standardized mean difference (SMD) using random-effects models at 95% confidence intervals (95% CI). Publication bias among studies was computed with Egger’s test and visualized using funnel plots. Results: Results indicated that dietary FSBM inclusion increased final body weight (BW) (SMD = 0.586, 95% CI: 0.221 to 0.951, p = 0.002) of broiler chickens, particularly in starter period (SMD = 0.691, 95% CL: 0.149 to 1.233, p = 0.013) while in the finisher period, the effect was weaker (SMD = 0.509, 95% CI: 0.015 to 1.004, p = 0.043). Average daily gain (ADG), feed intake (FI), and feed conversion ratio (FCR) were not affected with FSBM inclusion when compared to control. Subgroup analysis revealed that FI increased in starter period (SMD = 0.582, 95% CI: 0.037 to 1.128, p = 0.036). When considering types of microorganism as moderating variables in the subgroup analysis, we found that Aspergillus oryzae, mixed probiotics+bromelain protease, Bacillus subtilis, and Lactobacillus bacteria significantly increased ADG and FI (p<0.01). Additionally, either Bacillus subtilis+protease or Bacillus subtilis alone decreased FCR (p<0.001). However, meta-regression analysis showed that levels of FSBM inclusion had no effects on final BW (p = 0.502), ADG (p = 0.588), FI (p = 0.861), and FCR (p = 0.462). Conclusion: Substituting SBM in broiler chickens’ diet with FSBM improved BW of broiler chickens, especially in the starter period whereas the effects on ADG, FI, and FCR were mostly dependent on microbial strains used for fermentation. Objective: The present study aimed to quantify the effects of fermented soybean meal (FSBM) on broiler chickens’ performance by employing a meta-analysis approach.Methods: A total of 16 studies were included in the database after being systematically selected using a PRISMA protocol. Hedges’ <i>g</i> effect size was used to quantify pooled standardized mean difference (SMD) using random-effects models at 95% confidence intervals (95% CI). Publication bias among studies was computed with Egger’s test and visualized using funnel plots.Results: Results indicated that dietary FSBM inclusion increased final body weight (BW) (SMD = 0.586, 95% CI: 0.221 to 0.951, p = 0.002) of broiler chickens, particularly in starter period (SMD = 0.691, 95% CL: 0.149 to 1.233, p = 0.013) while in the finisher period, the effect was weaker (SMD = 0.509, 95% CI: 0.015 to 1.004, p = 0.043). Average daily gain (ADG), feed intake (FI), and feed conversion ratio (FCR) were not affected with FSBM inclusion when compared to control. Subgroup analysis revealed that FI increased in starter period (SMD = 0.582, 95% CI: 0.037 to 1.128, p = 0.036). When considering types of microorganism as moderating variables in the subgroup analysis, we found that <i>Aspergillus oryzae</i>, mixed probiotics+bromelain protease, <i>Bacillus subtilis</i>, and <i>Lactobacillus</i> bacteria significantly increased ADG and FI (p<0.01). Additionally, either <i>Bacillus subtilis</i>+protease or <i>Bacillus subtilis</i> alone decreased FCR (p<0.001). However, meta-regression analysis showed that levels of FSBM inclusion had no effects on final BW (p = 0.502), ADG (p = 0.588), FI (p = 0.861), and FCR (p = 0.462).Conclusion: Substituting SBM in broiler chickens’ diet with FSBM improved BW of broiler chickens, especially in the starter period whereas the effects on ADG, FI, and FCR were mostly dependent on microbial strains used for fermentation.

Relationships between dietary rumen-protected lysine and methionine with the lactational performance of dairy cows — A meta-analysis

Irawan Agung,Sofyan Ahmad,Wahyono Teguh,Harahap Muhammad Ainsyar,Febrisiantosa Andi,Sakti Awistaros Angger,Herdian Hendra,Jayanegara Anuraga 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.11

Objective: Our objective was to examine the relationships of supplemental rumen-protected lysine (RPL) or lysine + methionine (RPLM) on lactational performance, plasma amino acids (AA) concentration, and nitrogen use efficiency of lactating dairy cows by using a meta-analysis approach. Methods: A total of 56 articles comprising 77 experiments with either RPL or RPLM supplementation were selected and analyzed using a mixed model methodology by considering the treatments and other potential covariates as fixed effects and different experiments as random effects. Results: In early lactating cows, milk yield was linearly increased by RPL (β1 = 0.013; p< 0.001) and RPLM (β1 = 0.014; p<0.028) but 3.5% fat-corrected milk (FCM) and energycorrected milk (ECM) (kg/d) was increased by only RPL. RPL and RPLM did not affect dry matter intake (DMI) but positively increased (p<0.05) dairy efficiency (Milk yield/DMI and ECM/DMI). As a percentage, milk fat, protein, and lactose were unchanged by RPL or RPLM but the yield of all components was increased (p<0.05) by feeding RPL while only milk protein was increased by feeding RPLM. Plasma Lys concentration was linearly increased (p<0.05) with increasing supplemental RPL while plasma Met increased (p<0.05) by RPLM supplementation. The increase in plasma Lys had a strong linear relationship (R2 = 0.693 in the RPL dataset and R2 = 0.769 in the RPLM dataset) on milk protein synthesis (g/d) during early lactation. Nitrogen metabolism parameters were not affected by feeding RPL or RPLM, either top-dress or when supplemented to deficient diets. Lactation performance did not differ between AA-deficient or AA-adequate diets in response to RPL or RPLM supplementation. Conclusion: RPL or RPLM showed a positive linear relationship on the lactational performance of dairy cows whereas greater improvement effects were observed during early lactation. Supplementing RPL or RPLM is recommended on deficient-AA diet but not on adequate-AA diet.

Effect of  density between  intercropped sorghum and  stylosanthes on biomass production and quality under varying NPK fertilizer application rates

Dian Astuti,Bambang Suhartanto,Nafiatul Umami,Agung Irawan 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3

This field study was conducted to investigate the effect of spaced-row pattern and NPK fertilizer rates on productivity and quality of inter cropped sorghum and Stylosanthes guianensis. Three spaced-row pattern (S1 = 90x20 cm, S2 = 75x25 cm, and S3 = 60x30 cm, respectively) and NPK fertilizer application rates (N1 = 0 without NPK, N2 = 100 kg/ha, N3 = 200 kg/ha, respectively) were investigated under two local sorghum varieties (V1 = var. Kawali and V2 = var. Super-2) in a 2x3x3 split-split-plot design. Intercropped sorghum x stylosanthes in S3 resulted in higher dry matter (DM) content and biomass production than that in S1. NPK fertilization significantly increased DM and organic matter (OM) production by 16.2–115.0% and 16.1–115.2%, respectively, compared to those without NPK fertilization. Interaction effects of V x NPK and V x S x NPK were detected that contributing to the increase in biomass and nutrient quality of the forages. In vitro DM and OM digestibility rates for V1-S were higher than at V2-S. In conclusion, V2 produced a higher biomass, whereas V1 was superior in terms of nutrient quality. Spaced-row at 60x30 cm and 100 kg/ha of NPK were efficient in improving the productivity and nutrient quality of sorghum and stylosanthes.

Roles of Averrhoa bilimbi Extract in Increasing Serum Nitric Oxide Concentration and Vascular Dilatation of Ethanol-Induced Hypertensive Rats

Rondius Solfaine,Lailatul Muniroh,Sadarman,Apriza,Agung Irawan 한국식품영양과학회 2021 Preventive Nutrition and Food Science Vol.26 No.2

The considerably high incidence of cardiovascular disease in Indonesia has attracted scientists to investigate various plant and fruit extracts as preventive agents. Averrhoa bilimbi (AB) is rich in bioactive constituents that may be effective in preventing indicators of hypertension. This study evaluated the roles of AB extract in increasing serum nitric oxide (NO) concentration and vascular dilatation in ethanol-induced hypertensive rats. A total of 24 male Wistar rats (Rattus norvegicus) were divided equally into 4 treatment groups (n=6): P0 (control group, administered placebo); P1 [administered captopril 3 mg/kg body weight (BW) orally]; P2 (administered AB extract at 20 g/kg BW); and P3 (administered AB extract at 40 g/kg BW). The AB extract was obtained from fresh AB macerated in 96% ethanol and was subjected to bioactive compounds identification using thin layer chromatography. After pretreatment with ethanol for 15 days, treatments were administered daily for 14 days. All rats were measured for tail blood pressure by the tail-cuff method and NO concentrations by avidin-horseradish peroxidase sandwich-enzyme-linked immunosorbent assays. All rats were sacrificed to collect blood vessels for histopathology. The results showed that AB extracts contained flavonoids, saponins, polyphenols, essential oils, and anthraquinone. Treatment with AB extract at a dose of 40 mg/kg BW significantly increased NO concentrations (P<0.05). Histopathological analysis showed that AB extracts inhibited endothelial pyknosis, intimal body, and adventitial leukocyte infiltration of posterior vena cava blood vessels. These results suggest that the protective effect of AB extracts is associated with NO concentration in the blood by inhibiting blood vessel dysfunction.