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Ko, Ah-Reum,Hyun, Hye-Won,Min, Su-Ji,Kim, Ji-Eun Frontiers Media S.A. 2016 Frontiers in cellular neuroscience Vol.10 No.-
<P>The response and susceptibility to astroglial degenerations are relevant to the distinctive properties of astrocytes in a hemodynamic-independent manner following status epilepticus (SE). Since impaired mitochondrial fission plays an important role in mitosis, apoptosis and programmed necrosis, we investigated whether the unique pattern of mitochondrial dynamics is involved in the characteristics of astroglial death induced by SE. In the present study, SE induced astroglial apoptosis in the molecular layer of the dentate gyrus, accompanied by decreased mitochondrial length. In contrast, clasmatodendritic (autophagic) astrocytes in the CA1 region showed mitochondrial elongation induced by SE. Mdivi-1 (an inhibitor of mitochondrial fission) effectively attenuated astroglial apoptosis, but WY14643 (an enhancer of mitochondrial fission) aggravated it. In addition, Mdivi-1 accelerated clasmatodendritic changes in astrocytes. These regional specific mitochondrial dynamics in astrocytes were closely correlated with dynamin-related protein 1 (DRP1; a mitochondrial fission protein) phosphorylation, not optic atrophy 1 (OPA1; a mitochondrial fusion protein) expression. To the best of our knowledge, the present data demonstrate for the first time the novel role of DRP1-mediated mitochondrial fission in astroglial loss. Thus, the present findings suggest that the differential astroglial mitochondrial dynamics may participate in the distinct characteristics of astroglial death induced by SE.</P>
( Ah Reum Ko ),( Tae Cheon Kang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.9
In the present study, we addressed the question of whether treatment with mannitol, an osmotic diuretic, affects astrogliovascular responses to status epilepticus (SE). In saline-treated animals, astrocytes exhibited reactive astrogliosis in the CA1-3 regions 2-4 days after SE. In the mannitol-treated animals, a large astroglial empty zone was observed in the CA1 region 2days after SE. This astroglial loss was unrelated to vasogenic edema formation. There was no difference in SE-induced neuronal loss between saline- and mannitol-treated animals. Furthermore, mannitol treatment did not affect astroglial loss and vasogenic edema formation in the dentate gyrus and the piriform cortex. These findings suggest that mannitol treatment induces selective astroglial loss in the CA1 region independent of vasogenic edema formation following SE. These findings support the hypothesis that the susceptibility of astrocytes to SE is most likely due to the distinctive heterogeneity of astrocytes independent of hemodynamics. [BMB Reports 2015; 48(9): 507-512]
Ko Eun-Ji,Kim Hyunsu,Lee A-Reum,Jeon Kyung‑Yoon,Kim Ahran,Kim Do‑Hyung,Park Chan-Il,Choi Yung Hyun,Kim Suhkmann,Kim Heui-Soo,옥미선,차희재 한국유전학회 2021 Genes & Genomics Vol.43 No.11
Background Rock bream iridovirus (RBIV) is one of the most dangerous pathogens that causes the highest mortality in the aquaculture of rock bream (Oplegnathus fasciatus). Even though RBIV infection leads to huge economic loss, proteome studies on RBIV-infected rock bream have not been conducted to provide information about the diferential protein expression pattern by the host protection system. Objective The purpose of this study was to investigate the protein expression patterns in spleens of rock bream olive after infection by RBIV or mixed infection by RBIV and bacteria. Methods Depending on the infection intensity and sampling time point, fsh were divided into fve groups: uninfected healthy fsh at week 0 as the control (0C), heavily infected fsh at week 0 (0H), heavily mixed RBIV and bacterial infected fsh at week 0 (0MH), uninfected healthy fsh at week 3 (3C), and lightly infected fsh at week 3 (3L). Proteins were extracted from the spleens of infected rock bream. We used 2-DE analysis with LC–MS/MS to investigate proteome changes in infected rock bream. Results The results of the LC–MS/MS analyses showed diferent protein expression profles after infection. Proteins related to oxygen transport and energy generation, such as hemoglobin, beta-globin, and ATP synthase, were mostly expressed in the infected spleen. Whereas proteins involved in structure and cell movement, such as tubulin, myosin, actin binding proteins, and intermediate flament proteins, were down-regulated in the infected spleens. The protein expression profles between infection by RBIV and mixed infection by RBIV and bacteria showed similar patterns. Conclusions Our results indicated that infection by RBIV or mixed infection by RBIV and bacteria triggered energy generation and oxygen-transport, but cell migration and constructional changes in the spleen were extremely decreased.
Ko, Sunny,Park, Jae-Hyung,Lee, Ah-Reum,Kim, Eu-Gene,Kim, Ji-Young,Kawasaki, Ichiro,Shim, Yhong-Hee Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.2
Four new alleles, bn116, bn117, bn118, and bn119, on LG I were isolated in C. elegans with defects in germline stem cell proliferation. Using genetic mapping and snip-SNP mapping, bn116, bn117, bn118, and bn119 were located 5.0 cM, 1.3 cM, 2.3 cM, and 5.0 cM, respectively, to the right of dpy-5 on LG I. Further, bn116 and bn119 were grouped into the same complementation group by a complementation test. They are loss-of-function recessive alleles that produce homozygous sterile worms whose germ cells do not proliferate during larval development. However, the worms contained normal somatic gonadal structures including distal tip cells and gonadal sheath cells, suggesting that the defect in germline proliferation was not caused by the absence of somatic signaling. Although DAF-16 was localized to the nucleus in all four mutants, the life span was extended only in the three mutants except bn116. These results suggest that the defect in germline stem cell proliferation, the presence of normal somatic gonadal tissues, and DAF-16 nuclear translocation were sufficient for extending the lifespan of the bn117, bn118, and bn119 mutants, but not the bn116 mutant. Intriguingly, bn116 and bn119 were identified as two different mutations on the same gene, pab-1, which encodes a poly(A)-binding protein. Therefore, although the bn116 and bn119 mutations cause similar defects in germ cell proliferation, their effects on life span are different.
Ko, Byoung-Seob,Kang, Suna,Moon, Bo Reum,Ryuk, Jin Ah,Park, Sunmin Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-
<P>We investigated that the long-term consumption of the water (KME-W) and 70% ethanol (KME-E) mistletoe extracts had antidiabetic activities in partial pancreatectomized (Px) rats. Px rats were provided with a high-fat diet containing 0.6% KME-E, 0.6% KME-W, and 0.6% dextrin (control) for 8 weeks. As normal-control, Sham-operated rats were provided with 0.6% dextrin. In cell-based studies, the effects of its main terpenoids (betulin, betulinic acid, and oleanolic acid) on glucose metabolism were measured. Both KME-W and KME-E decreased epididymal fat mass by increasing fat oxidation in diabetic rats. KME-E but not KME-W exhibited greater potentiation of first-phase insulin secretion than the Px-control in a hyperglycemic clamp. KME-E also made <I>β</I>-cell mass greater than the control by increasing <I>β</I>-cell proliferation and decreasing its apoptosis. In a euglycemic-hyperinsulinemic clamp, whole-body glucose infusion rate and hepatic glucose output increased with potentiating hepatic insulin signaling in the following order: Px-control, KME-W, KME-E, and normal-control. Betulin potentiated insulin-stimulated glucose uptake via increased PPAR-<I>γ</I> activity and insulin signaling in 3T3-L1 adipocytes, whereas oleanolic acid enhanced glucose-stimulated insulin secretion and cell proliferation in insulinoma cells. In conclusion, KME-E prevented the deterioration of glucose metabolism in diabetic rats more effectively than KME-W and KME-E can be a better therapeutic agent for type 2 diabetes than KME-W.</P>
국내육성품종 심비디움 "그린볼"의 생장점 배양에 의한 PLB 및 소식물체 대량증식에 미치는 Hyponex, sucrose, 활성탄과 LED 효과
고정애 ( Ko Jeong Ae ),권아름 ( A Reum Kwon ),박종훈 ( Jong Hoon Park ) 전북대학교 농업과학기술연구소 2015 농업생명과학연구 Vol.46 No.2
To establish the mass production system of PLBs and regenerated plantlets, PLBs(Protocorm Like Body) derived from apical meristem of in vitro seedlings in domestic Cymbidium cv. Green Ball were cultured on Hyponex(6.5:6:19) media. We investigated the effect of optimal concentration of media, sucrose, activated charcoal and LEDs on the PLB production and plantlet regeneration via organogenesis. We first treated 1.27, 2.54, 3.81 g·L^{-1} of Hyponex and next added 10, 20, 30 g·L^{-1} of sucrose and 0.27, 0.54, 0.81 g·L^{-1} of activated charcoal to the Hyponex media during 50 days of culture. The greatest number of PLB(average 3.9) produced on 2.54 g·L^{-1} of Hyponex medium. PLB and shoots were vigorously multiplied on 2.54 g·L^{-1} of Hyponex with 2% concentration of sucrose. Also 0.27 g·L^{-1} was the optimal concentration of activated charcoal for the high efficiency survival rate and proliferation of PLB and plantlets. The other hand, in order to investigate the effect of light emitting diode(LED) lightings on PLB multiplication and mass production of plantlets, 0.2-0.3cm of PLBs were cultured on 2.54 g·L^{-1} Hyponex media with 2% sucrose and 0.27 g·L^{-1} activated charcoal in the growth chamber contained various LED lights. The PLB multiplication was highest under the red light as 6.3 and number of differentiated shoots were greatest as 3.6 after fifty days of culture. All of regenerated plantlets(2-3cm plant height) were grew under various lighting sources of LEDs (red, blue, red+blue, green and white) as well as fluorescent light. The rate of growth were differed according to the lightened kinds of LED and developmental stage of in vitro regenerated plantlets. After two hundred and ten days of culture, the plant height(12.7cm), the number of leaves(10.1), the length of leaves(10.4cm), the width of leaves(5.9mm), the number of roots(6.1) and the number of multiple shoots(5.8) were the most effective under the fluorescent lamp. However, green light was more longer than other light sources of the length of roots(4.9cm).