Insect Allergy

옥미선 한국생명과학회 1993 생명과학회지 Vol.3 No.4

알러젠은 크게 옥외 알러젠과 옥내 알러젠으로 중요하게 취급되는 종류로는 집먼지, 집먼지 진드기, 바퀴류, 애완동물의 털이나 분비물 등이 알려져 있다. 이 가운데 바퀴류는 곤충강(class insecta)의 직시목(Order Orthoptera)에 속하는 종으로 아프리카 에티오피아에서 기원하여 무역의 발달과 더불어 유럽, 아시아, 아메리카 대륙 등으로 전파된 것으로 알려져 있으며, 4000여종의 바퀴중 30여종이 사람과 함께 집에서 살고 있다. 본 총설에서느 바퀴류의 생태 및 공통항원과 종특이항원에 대하여 간략하게 언급하였다.

한국산 바퀴속의 형태학적 및 계통학적 연구

옥미선 慶北大學校 基礎科學硏究所 1991 慶北大學敎 基礎科學硏究所 硏究論文集 Vol.12 No.-

면역혈청학적 흡충류 검사법의 특이도 및 민감도 비교

옥미선 고신대학교(의대) 고신대학교 의과대학 학술지 1992 고신대학교 의과대학 학술지 Vol.8 No.1

-Abstract- To study the specificity and the sensitivity of immunodiagnostic tests for 3 closely related species of trematodes, clonorchis sinensis. Paragonimus westermani, Fasciola hepatica were used as antigens. Antisera of 13 clonorchiasis patients, 5 paragonimiasis patients, 1 fascioliasis patients, and 11 normal adults human sera were applied. Clonorchis antigen and antibody reaction in double-diffusion was weak, and only 23% among patients was positive. But paragonimiasis antisera-antigen reaction was very clear and 4 of 5 was positive. Clonorchiasis antisera-antisera was 100% positive in micro-ELISA, but clonorchis antigen, paragonimus antigen, fasciola antigen-fascioliasis antisera reaction was also 100% positive. In dot-ELISA, clonorchis antigen-antisera was 11 patients positive among 13 patients, but reaction of clonorchis antigen-paragonimus antisera was also 3 positive among 5 patients. Paragonimus antigen clonorchis antibody never reacted, but 1 of 5 normal human sera reacted with clonorchis and paragonimus antigen. And fasciola antigen made cross-reaction with paragonimiasis patient sera and 10 of 13 clonorchiasis patient sera. In this experiments, although there was no cross-reactivity in double-diffusion, sensitivity was low. Micro and Dot-ELISA had high sensitivity, but specificity was low. And it was considered that the apprepriate mixture of 3 kinds of test will produce the good results.

한국산 Blattella germanica L. 와 Blattella nipponica Asahina의 핵형분석

옥미선,김강련 高神大學 醫學部 1989 高神大學醫學部 論文集 Vol.5 No.2

고래회충 유충의 충체 및 분비 배설 단백질의 가수분해효소 활성 분석

옥미선 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background and Objectives : Anisakis simplex L3 (third-stage larva) is the most prevalent species causing anisakidosis which is associated with consumption of raw or insufficiently processed fish or cephalopods. Adult worm of A. simplex inhabits in the stomach of cetaceans and pinnipeds such as whales, porpoises, and seals. Eggs, passed in the feces of the adult worm in marine mammals, hatch to liberate larvae, which then can be ingested by marine crustaceans. The third-stage larvae in the crustaceans are eaten by various species of fish or cephalopods of paratenic or transport hosts. Anisakidosis can cause not only direct tissue damage but also severe allergic response caused by larvae as well as by excretory-secretory (ES) products. The objectives of this study was to check the pattern of hydrolase activity from extracts of L3, L4 stages and ES products of A. simplex larvae. Materials and Methods : A. simplex L3 were collected from mackerels (Scomber japonicus). The third and fourth-stage larval extracts were obtained by homogenising and centrifugation. After A. simplex L3 were kept in PBS for 9 consecutive days, the supernatant was used as the excretory-secretory products. To identify the hydrolase activity in larval extract and ES products, SDS-PAGE and API ZYM method were carried out. Results : Six common positive enzymes were detected in L3, L4 and ES products belonging to four general groups as follows: (1) phosphatase group: alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase; (2) esterase group: esterase lipase (C8); (3) protease group: leucine arylamidase; and (4) glucosidase group: α-glucosidase. ES products was relatively rich in trypsin and α-chymotrypsin, apart from the common positive hydrolases. Conclusions : A. simplex L3, L4 extract and ES products contain several kinds of hydrolases of phosphatase, esterase, protease and glucosidase group, which appear to be important in larval development and host–-parasite interaction.

한국산 Blattella germanica L.와 Blattella nipponica Asahina의 핵형분석

옥미선,김강련 고신대학교 의학부 1989 高神大學校 醫學部 論文集 Vol.5 No.2

The present study was performed to compare the karyotype of two species of the cockroaches, Blattella germanica L. and Blattella nipponica Asahina in Korea. The chromosome of two species of the cookroaches was observed by acetolacticorcein stain and giemsa stain method. The haploid chromosome number is 11 and 12 in male. In metaphase-Ⅱ, sex chromosome is metacentric and very small The results suggest that duploid chromosome number of B. germania and B. nipponica is 23 and 24. The sex chromosome of two species is XO type. The morphology of chromosome is metacentric or submetacentric.

旴吸蟲 感染 마우스의 血淸과 淋巴系細胞에 의한 色疫移入

玉美善 고신대학교 의학부 1988 高神大學校 醫學部 論文集 Vol.4 No.1

To study the transfer of immunity against the Clonorchis sinensis, the oriental liver fluke, in ICR mice, 30 donor mice were received per os and intraperitoneally with 20 metacercariae and excysted larvae. All donor mice were killed by the neck dislocatin after 30 days, and then serum and lymphoid cells were made from each donor group. Four groups of the recipient mice were immunized with serum and lymphoid cells from the donor group I and Ⅱ. 1 day after the primary immunization, all recipient mice were challenged orally with 20 metacercariae of C. sinensis, and killed 30 days after the challenge infection. The obtained results from this study were as follows. 1. The serum provided from the donor group I(per os infection) and II(I. p. infection) did not transfer the immunity to the same kind of mice. 2. The lymphoid cells obtained from the donor group I also could not protect the recipient mice from the challenge infection to worm burden and Eggs Per Gram in the feces. 3. Significant protection against the challenge infection with 20 metacercariae of C. sinensis was conferred on the same kind of ICR mice by the passive transfer of the lymphoid cells derived from the donor group II. 4. Number of plaque forming cells per spleen (PFC/spleen) accorded with the results of the transfer study.

면역혈청학적 흡충류 검사법의 특이도 및 민감도 비교

옥미선 고신대학교 의학부 1992 高神大學校 醫學部 論文集 Vol.8 No.1

Double-Diffusion, Micro-ELISA, Dot-ELISA 등의 방법으로 3종의 흡출류, 간흡충, 폐흡충, 간질을 진단한 결과 특이도는 Double-Diffusion에서 높은 것으로 나타났으나 민감도는 떨어졌고, Micro와 Dot-ELISA는 민감도는 높았으나 특이도가 낮은 것으로 나타났다. To study the specificity and the sensitivity of immunodiagnostictests for 3 closely related species of trematodes, Clonorcbissinensis, Paragonimus westermani, Fasciola bepatica were used as antigens. Antisera of 13 clonorchiasis patients, 5 paragonimiasis patients, 1 fascioliasis patients, and 11 normal adults human sera were applied. Clonorchis antigen and antibody reaction in double-diffusion was weak, and only 23% among patients was positive. But paragonimiasis antisera-antigen reaction was very clear and 4 of 5 was positive. Clonorchiasis antisera-antisera was 100% positive in micro-ELISA, but clonorchis antigen, paragonimus antigen, fasciola antigen-fascioliasis antisera reaction was also 100% positive. In dot-ELISA, clonorchis antigen-antisera was 11 patients positive among 13 patients, but reaction of clonorchis antigen-paragonimus antisera was also 3 positive among 5 patients. Paragonimus antigen-clonorchis antibody never reacted, but 1 of 5 normal human sera reacted with clonorchis and paragonimus antigen. And fasciola antigen made cross-reaction with paragonimiasis patient sera and 10 of 13 clonorchiasis patient sera. In this experiments, although there was no cross-reactivity in double-diffusion, sensitivity was low. Micro and Dot-ELISA had high sensitivity, but specificity was low. And it was considered that the apprepriate mixture of 3 kinds of test will produce the good results.

세월이 약이겠지요

옥미선 샘터사 2000 월간 샘터 Vol.31 No.10

肝吸蟲 感染 마우스의 血淸과 淋巴系細胞에 의한 色疫移入

玉美善 高神大學 醫學部 1988 高神大學醫學部 論文集 Vol.4 No.1