Bulimus striatulus에 對한 Insecticides의 殺滅效果

李周植(Zoo-Shik Lee),金昌煥(Chang-Whan Kim) 대한기생충학열대의학회 1963 The Korean Journal of Parasitology Vol.1 No.1

생화학적 요인이 세균 포자모에 미치는 영향에 관한 연구

이주식(Zoo Shik Lee),강철희(Chul Hee Kang),이오형(Oh Hyoung Lee),변상숙(Sang Suk Byon) 한국미생물생명공학회 1984 한국미생물·생명공학회지 Vol.12 No.2

성장 및 포자형성 중인 Bacillus subtilis SNU816의 SP816 박테리오파아지에 대한 감수성에 관하여

이오형,이주식,Lee, Oh-hyoung,Lee, Zoo-Shik 한국미생물학회 1984 미생물학회지 Vol.22 No.2

The changes of susceptibility of Bacillus subtilis SNU816 to bacteriophage SP816 were investigated. When B. sutilis SNU816 cells were infected by the phage during vegetative growth, rapid lysis was observed. But when they were infected after late logarismic phase, they were resistant to phage infection. Since asporogenic culture of this strain was invariably lysed regardless of time of infection, the arrest of phage multiplication seemed to be caused by sporulation. In reality, the arrest of phage multiplication occurred at early stage of sporulation. Electron microscopy revealed that the arrest of phage multiplication occurred just prior to or during septum formation (stage II sporulation).

Bacillus subtilis SNU 816의 Phage에 대한 전자현미경적 관찰

안경준,이주식,이웅직,Ahn, Kyung-Joon,Lee, Zoo-Shik,Lee, Woong-Jik 한국현미경학회 1982 Applied microscopy Vol.12 No.1

The phages of Bacillus subtilis SNU 816 were observed by electron microscope with their host. The results are as follows; The phage had hexagonal head, contractile tail sheath and base plate with six tail fibers. During the lysis of bacteria due to attack of phage, dissolution of flagella were initiated from distal end of flagella.

식품자원연구실편 : 마늘성분이 Bacillus subtilis S. N. U. 816 균의 발육에 미치는 영향

함승시(Seung Shi Ham),이주식(Zoo Shik Lee),피재호(Jae Ho Phee),김복란(Bok Ran Kim) 강원대학교 농업과학연구소 1989 강원 농업생명환경연구 Vol.3 No.-

한국 미생물 자원의 개발연구 : 특히 유용 미생물주(균주)의 분리와 응용에 관한 연구 The Research on the Isolation and Application of Utilizable Microorganisms Related to Jeonju Materials

이주식,백승화,이오형 Natural Science Research Institute JEONJU UNIVERSI 1987 전주대학교 자연과학연구소 학술논문집 Vol.2 No.2

The isolated strains of microbes from some hay, water of lake, marketing milk and Tachju brewing in Jeonju area. Were as follows Isolated 10 strains of Bacillus group, 2 strainins of Chlorella group, 2 strains of Lactobacillus group, 2 strains of Saccharomyces grpup and other microbes and determinated 4 strains in the 20 strains and application of utlizable microorganisms studies on the strains or pure cultual materials. Microbiological and biochemical examined for each organism. Its Bacillus species, Lactobacillus species, Chlorella species and Saccharomyces species were as follows. Bacillus species in Bacillus subtilis natto like strain. In addition, some of these strains were prepared to show different responses to the biotin added medium. Therefore our primary purpose was to examine the possible effects of glutamic acid, biotin and boiling soybean extract on the growth or sporulation of one these strains. It is a novel finding that biothin and soybean extrat affects on its sporulation. Glulamic acid was required for both its growth and sporulation. Chlorella species is Chlorella ellipsoidea like strain. The optimum cultural conditions and composition of the medium for wild Chlorella ellipsoidea were studies as follow. The addition than in control in concentration of the nitrogen sources high presented the effective result in the increasing order of meat extract, peptone amino, acid. Lactobacillus species is Lactobacillus casei like. Lactobacillus casei was isolated relatively high temperature and high salt contend and mesothermophilic condition. In addition, among, J-1, J-2, J-3 strains isolated together from above samples, J-2 was prominant in producing typical curd within 20 hours. Saccharomyces species is Saccharomyces cerevisiae like. When Sacchromyces cerevisiae like was used as a sole yeast source, this was dominated soon after it. But in this case Hansennula, Candida, Pichia, Torulopsis etc. were alse found out. This fact indicates that many kinds of yeast species were related with the alcohol fermentation. Biochemical studies on Cell-bound a-amylase in Bacillus subtilis like. Extracelluiar and Cell-bound a-amylase were purified separately and some of their biochemical properties examined. The amount of extracellular a-amylase produced by bacteria is known to very greatly with growth conditions. Extracellular a-amylase activity was more inhibited than cell bound a-amylase by metal ions tested.

ANTIGROWTH VIRUS(THIRD VIRUS OF SWINE)에 關한 硏究 : Ⅱ. 豚發育障碍症(Antigrowth disease of swine)와 仔豚感冒(Swine influenza)의 韓國內分布 및 豫防試驗에 關하여 Ⅱ. About the distribution and the preventive experiments of the antigrowth disease and influenza of swine

李周植 서울대학교 1957 서울대학교 論文集 Vol.5 No.-

In order to know the distribution of antigrowth virus of swine in Korea, I exmined 61 samples of swine serum sent from 11 provinces of korea with neutralizing method and found 9 positive samples : One from kyung-ki province, two from Chun-Book prov, one from Chun-Nam prov., two from Whang-Hai prov, One from Kyung-Boa ok prov, one from Kyung-Nam prov, and one from Han-Nam prov. Aiming at detecting contaminated areas by this disease, I had neurtalizing tests for 79 swines 68% wich got from Tong-Lai country of Kyung-Nam prov. and it of wich from Chin-Yang, Masan country of the same prov, and got 4 positive dat. Inten ding to clearify the distribution of influenza of swine in korea, I testes 124 samples sent from 12 Provinces with the neutralizing method, and found 4 positive samples. I also examined the samples from Chin-Yang and, Masan Country of Kyung-Nam province with positive data. From above montioned results, I think, it was revealed that the antigrowth virus and influenza virus of swine were widely distributed all over korea. As prophylactic experiments, I proved that the single-high grade-immuned serum of antigrowth virus of swine had and immunizing ability till 10^(-2) of serum, and that the single-highgrade immured serum of influenza virus of swine had and immunizing ability till 5-folds of serum. With equivelent mixture of these two kinds of serum I made field examinations at the breeding place of swine of Ham-Book province in spring and at the Tong-Lai pigfarm of Kyung-Nam Province in autumn with following notable effects: The former reduced the annual mortality of young swines fom 40·50% to about 10%, and the latter from 70-80% to about 25%. I therefore think that with the mixture of these two immuned sera we can prevent young seines from the infection of these viruses vausing them to die. The conclusion is as follows: 1, The antigrowth virus of swine and influenza virus of swine are widely distributed over whole korea. 2, The equivalent mixture of these two high grade immuned sera is most effective on preventing young swine; from disturbances of growth and death.

加工大麥(平麥) 醱酵에 使用된 Bacillus subtilis SNU 816의 蒐集과 生物學的 性狀에 關한 硏究

李周植,李吾炯 Natural Science Research Institute JEONJU UNIVERSI 1986 전주대학교 자연과학연구소 학술논문집 Vol.1 No.1

The "Natto" a foodstuff made from soybeen fermentation has been produced and consumed about 300, 000 ton every year in Japan. However, its production and consumption are in fact poor in korea regared as a developing country. It is thought that the production of spore liquid of fermentation microorganism and the management of the strain are the important subjects. Microbiological investigations on the spore and mucin formation were carried out using, the strain of Bacillus subtilis SNU 816 in order to solve the afere mentioned subjects. The results are sunmarized B.subtilis SNU 816 was classified from a viewpoint of physiological characteristics as a strain of B. subtills by means of Bergey's manual (1957). Solution of soybeen extracted with 10% NaC1, pH6.4, was very suitable for the spore formation of the strain . The cultures keeping at. 37℃, 40℃ and 50℃ in increasing order were more favorable especilly for the mucin formation of the spore from, though the from lipuid of vegetative form was not suiltable. The starter for the flat barley fermentation should be spore from lipuid of the strain and same factors were related for the spore and mucin formation of the strain, but there were many differences in the results by fermantation methods. The effects of glutamic acid and biotin on the growth and sporulation of Bacillus subtilis SNU 816 were investigated. When B. subtilis SNU 816 was cultivated in glucose-minenal salts medium(Glc medium) with week buffer action, the growth rate and percentage of sporulation were partially increased by sole addition of glutamic acid (Glc+Glu medium) or biotin(Glc + Bio medium). On the ather hand when both glutamic acid and biotin were added to the medium(Glc+Bio+Glu medium), considerable increases in both growth rate and percentage of sporultion were observed. The approximate doubling times in each Glc, Glc+Glu, Glc+Bio, and Glc+Bio+Glu medium were 4 hours, 3 hours, 1.5 hour, 0.7 hour, respectively. Percentages of sporulation after 18 hours cultivation in each Glc, Glc+Glu, Glc+Bio, and Glc+Bio+Glu medium were 0.01% 0.13% 1.4% and 43%, respectively. The lag periods in each Glc, Glc+Bio, Glc+Glu, and Glc+Bio+Glu medium were about 36.2 hours, 29.8 hours, 12.3 hours, and 11 hours, respecitively. These results indicated that the addition of glutamic acid to Glc medium was much more effective in the reduction of the ag period that of biotin. The growth rate in Glc medium was proportionally increased according as the concentration of biotin added to the medium was increased until it was reached to 0.02mg/ml. Above that concentration, no more addition-effect was observed. The requirement of this strain for biotin was obvious in the Glo+Glu medium whis initial pH5.0. Because of the low pH value of the medium, the growth was considerably refarded. But when the biotin was added to this medium, ncrmal growth was resfored, and the pH of the medium was rapidly increased. These results implicated that the addition of biotin would enable this strain to use glutamic acid rapidly from the medium. It was concluded that glutamic acid and biotin were needed for this strain to reduce the lag periods, and to achieve optimal growth and sporulation.

夏季 腦炎 發生地의 모기 調査

李周植 서울대학교 1957 서울대학교 論文集 Vol.6 No.-

We collected and classified the abults and larvae of mosquitoes, intermediary insects of summer encephalitis at Kai-Chung area Cholla Book Do province, where the encephalitis was endemic in 1952 and got following results. 1. We collected 2 kinds of nosquitoes including many Aedes dorsalis the existence of which had never been reported before. 2. Among these mosquitoes, Anopheles sinensis prevailed in number and had prodominent infestation even in september, though it was accepted previously that heavy infestation of Anopheles sinensis was seen in august. 3. A virus of the encephalitis could be isolated from Culex tritaeniorhynchus. 4. The heavy infestation of Anopheles sinensis, Aedes vexans and Aedes dorsalis in september is very important fact to be recognized.

CHLORELLA 細胞의 微細構造에 關한 硏究 : 特히 Pyrenoid와 細胞壁構造에 關하여

李周植 서울대학교 1966 서울대학교 論文集 Vol.17 No.-

JE strain isolated in Japan, Y 511 and Y 185 strain in Korea were examined to observe the structures of pyrenoid and cell wall and relation to strach grain formation at the ultrastructure level. The development of pyrenoid of chlorella species from the time of its initiation and its subdetail sequent activities is decribe in some picture. Close correlation between the finding of light microsocpy and electronmicroscopy is proved. The pyreniod is a dynamic organelle which continues to change its appearance throughout the development of the chlorella cell. The starch grains are continuously formed by deposition of carbohydrate within the chloroplast with the aid of pyrenoid factors. Some parental starch grains are passed on the daughter cell during cell division. The cellular boundary of chlorella species contain intermediate layer between the fibrilar cellulose wall and the out capsule layer.