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황소련,조지훈,신경민,장윤영,김지연,여경욱,김형아,허용,Hwang, So Ryeon,Jo, Ji Hoon,Shin, Kyeong Min,Jang, Yun Young,Kim, Ji Youn,Yeo, Kyeong Uk,Kim, Hyoung Ah,Heo, Yong 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.6
Objectives: This study was undertaken in order to evaluate a potential mechanism involved in gastro-intestinal problems observed in autistic subjects and uses an animal model of autism investigation. Methods: BTBR T+tf/J, a mouse strain with typical socio-behavioral characteristics of autistic subjects and FVB mice with highly social behaviors as the control strain were used. Both genders of mice aged three weeks and six months were used from four separate litters for each strain. Serum was prepared following cardiac puncture, and mesenteric lymph nodes were collected for in vitro stimulation and enumeration of major immune cell proportion. Results: The level of serum IgA was significantly enhanced in six-month-old BTBR mice compared with three-week-old BTBR, which was not observed with the FVB control mice. The serum IgE level was also higher among BTBR mice than among age-sex matched FVB mice, respectively. Considering the ratio of interleukin-4 vs interferon-gamma production from mesenteric lymph node T cells, skewedness toward type-2 reactivities was observed. In addition, the proportion of B cells in mesenteric lymph nodes was significantly higher in BTBR mice than in FVB mice. Conclusion: Upregulation of mucosal immunity related with enhanced type-2 immune reactivity observed in BTBR mice could be involved with the etiology of gastro-intestinal abnormalities in autism.
연구논문 : 정각질세포주의 피부감작물질 검색 관련 효용성 분석
김소남 ( So Nam Kim ),여경욱 ( Kyeong Uk Yeo ),황소련 ( So Ryeon Hwang ),조지훈 ( Ji Hoon Jo ),손수정 ( Soo Jung Sohn ),염영나 ( Young Na Yum ),이용경 ( Yong Kyung Lee ),허용 ( Yong Heo ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.
김소남 ( So Nam Kim ),여경욱 ( Kyeong Uk Yeo ),황소련 ( So Ryeon Hwang ),조지훈 ( Ji Hoon Jo ),손수정 ( Soo Jung Sohn ),염영나 ( Young Na Yum ),이용경 ( Yong Kyung Lee ),허용 ( Yong Heo ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.
피부감작능 평가를 위한 BrdU-FCM 국소림프절시험법 기법 이전을 위한 제 조건
여경욱 ( Kyeong Uk Yeo ),김지연 ( Ji Youn Kim ),황소련 ( So Ryeon Hwang ),임경민 ( Kyung Min Lim ),정경미 ( Kyung Mi Jung ),장원희 ( Won Hee Jang ),허용 ( Yong Heo ) 한국동물실험대체법학회 2012 동물실험대체법학회지 Vol.6 No.1
Local lymph node assay (LLNA) using mice has been adopted as an alternative model for testing skin sensitization to chemicals. Modification of LLNA was recently reported through combination of BrdU incorporation and flow cytometric analysis, which was designated as LLNA:BrdU-FCM. We introduce how LLNA:BrdU-FCM could be successfully established in a naive laboratory where the method was never experienced. Nine week old female BALB/c mice, 5 mice per group, were used and grouped into non-treatment, BrdU only-treatment, acetone-olive oil (AOO) vehicle-treatment, and 5, 10, 25% α-hexylcinamaldehyde (HCA) positive control. BrdU solution was injected intraperitoneally one day after three consecutive application of AOO or HCA on dorsum area of both ears. Auricular lymph node was collected from both ears one day after the BrdU injection. Thereafter flow cytometric staining procedures were followed. Various parameters related with calculation of stimulation index were analyzed including number of lymphocytes, BrdU incorporation%, and number of BrdU incorporated lymphocytes. The critical procedures for successful establishment were as followings; quality control of every quantitative apparatus, appropriate application of test compounds on dorsum of mouse ear, precise injection of BrdU, accurate preparation of single cell suspension and counting of lymph node cells, and experienced operation of flow cytometer. Face-to-face training from an experienced lead laboratory and preparation of very detailed standard operational procedures could play the most important role for the establishment. Based on Performance Standards for Assessment of Proposed Similar or Modified Test Methods by OECD, the LLNA:BrdU-FCM could be a good alternative method for screening skin sensitizers.
경기 일부 지역 양돈 축산인들의 호흡기 알레르기 관련 면역학적 지표 분석
김지연 ( Ji Youn Kim ),김광호 ( Kwang Ho Kim ),황소련 ( So Ryeon Hwang ),여경욱 ( Kyeong Uk Yeo ),김형아 ( Hyoung Ah Kim ),허용 ( Yong Heo ) 한국산업위생학회 2012 한국산업보건학회지 Vol.22 No.4
Objectives: Animal husbandry workers could be exposed to various work hazards including toxic gases, chemicals such as pesticides or organic dust. Immunological evaluation focusing on respiratory allergic hypersensitivity occurrence was undertaken for swine farm workers as a part of the study on immunologic status of dairy barn, swine confinement, and poultry farm workers. Materials aanndd Methods: Peripheral bloods were collected from 25 workers at the year of 2001 and 12 workers at the year of 2012 from swine farms located at Gyeonggi province, Korea. Seven adults not involved with animal husbandry were recruited at the year of 2001 from the same residential area as the swine farm workers`. Level of plasma IgE and 20 respiratory allergen-specific IgE were evaluated using commercially available ELISA kit. Results: Plasma IgE level was approximately five-fold higher in the swine farm workers regardless of the sampling year than the control subjects. Plant allergens from outdoor environments such as golden rod, pigweed, Russian thistle, or ragweed were the major allergens with positive reaction(allergen specific IgE≥0.7 IU/mL) for the swine farm workers at 2001 year. Meanwhile, house dust mite(Dermatophagoides farinae, D. pteronyssinus) and cockroach, typical indoor allergens in Korea, were the major respiratory allergens for the swine farm workers at 2012 year. Conclusions: Overall, even though our results are primitive, the results suggest that immunological function of swine farm workers could be modulated toward type-2 reactivity.