도재 라미네이트와 복합레진 수복 시 치간이개 양에 따른 접착계면의 응력분포에 관한 3차원 유한요소법적 연구

홍준배,탁승민,백승호,조병훈 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

본 연구에서는, 삼차원 유한요소분석법을 이용하여 복합레진 및 도재 라미네이트로 전치부 치간이개를 폐쇄할 때, 치간이개의 간격과 수복방법이 수복물의 접착계면에서의 응력분포에 미치는 영향을 비교하였다. 복합레진 혹은 도재 라미네이트로 수복된 모습의 유한요소모델을 형성하여 접착계면에서의 von Mises stress 분포 양태를 분석하였다. 수복형태는 크게 네 가지 범주를 고려하였다. Type I, 복합레진으로 인접면을 최소한으로 수복하는 경우; Type II, 치아 삭제 없이 복합레진으로 순면의 절반까지 수복하는 경우; Type III, 치아삭제 후복합레진으로 순면의 2/3까지 수복하는 경우; Type IV, 도재 라미네이트로 수복하는 경우 이 실험의 한계 내에서 다음과 같은 결론을 내릴 수 있었다. 모든 수복형태에 대해 치간이개 간격이 넓어질수록 접착계면에서 발생하는 최대응력 값은 증가하였으며, 도재 라미네이트로 치간이개 부위를 수복한 경우(Type IV)가 복합레진으로 수복한 경우(Type I, II, III)에 비해 높은 최대응력값을 나타내었다. 그러나 치간이개 간격이 넓어질수록 도재 라미네이트와 복합레진에서 발생하는 최대응력값의 차리는 감소하였으며, 치간이개 간격의 증가에 따른 최대응력값의 증가율은 도재 라미네이트가 복합레진으로 수복하는 경우보다 낮았다. This study evaluated the influence of the type of restoration and the amount of interdental spacing on the stress distribution in maxillary central incisors restored by means of porcelain laminate veneers and direct composite resin restorations. Three-dimensional finite element models were fabricated to represent different types of restorations. Four clinical situations were considered. Type I, closing diastema using composite resin. Labial border of composite resin was extended just enough to cover the interdental space; Type II, closing diastema using composite resin without reduction of labial surface. Labial border of composite resin was extended distally to cover the half of the total labial surface; Type III, closing diastema using composite resin with reduction of labial surface. Labial border of the preparation and restored composite resin was extended distally two-thirds of the total labial surface; Type IV, closing diastema using porcelain laminate veneer with a feathered-edge preparation technique. Four different interdental spaces (1.0, 2.0. 3.0, 4.0 mm) were applied for each type of restorations. For all types of restoration, adding the width of free extension of the porcelain laminate veneer and composite resin increased the stress occurred at the bonding layer. The maximum stress values observed at the bonding layer of Type IV were higher than that of Type I, Ⅱ and Ⅲ. However, the increasing rate of maximum stress value of Type IV was lower than that of Type I, Ⅱ and Ⅲ.

서울시내 종합 병원 공기중의 미생물 분포

홍준배,정윤희,장윤희 한국환경보건학회 2003 한국환경보건학회지 Vol.29 No.1

This study was carried out to investigate the airborne microbial pollution in hospital environment. Using a mechanical air sampler, microbiological samples were taken from intensive care unit, general ward room, patients waiting room and outdoor of 20 hospitals in Seoul, Korea. The concentration of airborne bacteria and fungi ranged 97-410 cfu/m3 and 37-77 cfu/m3, respectively and patients waiting room had highest bacterial count. 18 genera of molds were identified and the most frequently recovered molds were Aspergillus, followed by Penicillium, Alternaria and Cladosporium. Among Staphylococcus species, S. haemolyticus and S. epidermidis were predominant and 47% of Staphylococcus species were isolated from intensive care unit. 서울에 위치한 20개 종합병원의 중환자실, 일반병동 입원실, 환자대기실과 병원 실외 4곳 공기중의 일반 세균수와 곰팡이수를 측정하였으며, 기회 감염균을 검출하였다. 97-410 cfu/m3의 일반세균수와 37-77 cfu/m3의 진균이 검출되었으며 중환자실이 가장 낮은 미생물의 오염도를 보였으며 환자대기실이 가장 높은 오염도를 나타내었다. 18종의 진균이 분리되었으며 Aspergillus spp.가 가장 높은 빈도로 검출되었으며, 그 다음으로 Penicillium, Alternaria, Cladosporium 속 등이 검출되었다. Staphylococcus spp. 중에서는 S. haemolyticus와 S. epidermis가 가장 높은 빈도로 검출되었으며 검출된 Staphylococcus spp. 중 47%가 중환자실에서 검출되었다.I. Introduction

III. 소비자와 항생제 내성

홍준배 대한수의사회 2005 대한수의사회지 Vol.41 No.6

식품에서의 Campylobacter spp., C. perfringens 항생제 내성

홍준배 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-

시중유통 견과류의 총아플라톡신, 오크라톡신 A, 제랄레논, 데옥시니발레놀, T-2 독소의 오염도 조사

홍준배,박건택 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.1

In the current study, 109 commercial nut samples were collected from different Korean markets and analyzed for the contamination of 5 different mycotoxins (aflatoxin, ochratoxin A, deoxynivalenol, zearalenone, and T-2 toxin) using ELISA kits. The results revealed that the most frequently detected mycotoxin was zearalenone (n=36, 33%), followed by aflatoxin (n=31, 28.4%) and ochratoxin A (n=30, 27.5%). Deoxynivalenol and T-2 toxin were also detected in 22 (20.3%) samples, respectively. Among 109 nut samples, 33 samples (30.3%) were contami-nated only with one kind of mycotoxin, whereas 43 samples had at least 2 kinds of mycotoxins. Two samples were contaminated with as many as 4 different mycotoxins, and they were both walnuts. Although the monitoring results revealed the amount of aflatoxin contamination was under the safety criteria, there is no current safety guideline for other kinds of mycotoxins or multiple contaminations in Korea. Therefore, further studies should be performed to reveal the distribution of mycotoxin in different foods and propose appropriate safety guidelines for Korean markets.

Microbial Contamination in Kitchens and Refrigerators of Korea Households

홍준배,임승용 한국식품위생안전성학회 2015 한국식품위생안전성학회지 Vol.30 No.4

The objectives of this study were to investigate the microbial contamination level of domestic kitchen environments and to provide information to improve food safety in 50 domestic house kitchens located in Seoul, Incheon, and Gyeonggi-do. Dishcloth, chopping board, and refrigerator swabs were examined for the presence of coliforms, Salmonella spp., Campylobacter jejuni/coli, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus. The means and standard deviations of coliform counts for dishcloths was 4.8±1.84 log CFU/100g, chopping boards, and refrigerator drawers were 4.04±1.53, 4.11±1.65 log CFU/100cm2, respectively. Salmonella spp. and Campylobacter jejuni/coli were not detected in all samples. E. coli were detected in 3 on the dishcloths and 1 of 50 samples in the refrigerator drawer. Listeria monocytogenes was detected in the drawer of the refrigerator in 2 of 50 samples. In the case of Staphylococcus aureus, the detection on dishcloths, chopping boards, and drawers in refrigerators was 21, 12, and 14 of 50 samples, respectively. The results of microbiological tests of domestic kitchen utensils can be used to emphasize the importance of the sanitary conditions in domestic kitchen environments.

소비자가 바라는 안전한 어린이 식품

홍준배(Joonbae Hong) 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

생활환경과 실내공기의 미생물학적 오염에 관한연구

정윤희,홍준배,장윤희 한국환경보건학회 2001 한국환경보건학회지 Vol.27 No.2

This study was carried out to investigate the microbial characteristics of the urban air pollution in the subway stations, streets, department stores, wholesale markets, underground shopping centers, buildings, parks, houses and apartments in the Seoul and the suburbs area. Total cell count, total mold count and the presence of opportunistic pathogens(Streptococcus pneumoniae, Klebsiella pneumoniae, Aspergillus spp., Penicillium spp.) were evaluated determine the microbial air quality. Total cell count and mold count of indoor air in the houses and apartments were 2.9X102-6.3X102 cfu/㎥ and 60-1.8X102cfu/㎥, respectively, and the department stores and wholesale markets had much lower cell count than the houses and apartments. Ground level of commercial stations were 2.6 fold higher than the general subway stations, and Apergillus spp. and Penicillium spp. which could cause the bronchus and lung diseases were detected 17 sampling site out of 45. Dust were collected from the commercial facilities and houses, and total cell and mold count of the dust were 4.3X103-1.7X106 cfu/g and 2.3X103-6.5 X104cfu/g, respectively. Therefore the dust might be one of the main reservoir of microorganims

시중 유통 중인 한우와 수입쇠고기의 유전자 비교 및 위생시험

서정희,홍준배,정윤희,김말남 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.4

Recently there has been an increasing amount of foreign livestock products distributed in the domestic market due to the market opening. Some vicious dealers sell the foreign beef in the trade name of the native beef during the final distribution step to arouse the social criticism frequently. In this report, we investigated a method to distinguish the native beef from the foreign one scientifically using the PCR-RAPD, a recent gene technique. Hygienical safety was also examined using a microbiological test for toxicity of Escherichia coli O157:H7 and the food poisoning bacteria. The conditions of DNA amplification for the PCR analysis were 1 × Taq polymerise buffer, 1.5 mM MgCl₂, 50 uM dNTP, 100 ng primers, 2.5 unit Taq polymerise and 5-20 ng template DNA, with the final volume of 50 Etl. The size of the amplified product was detected mostly in the range of 0.5-2.0 kbp. The size of DNA, gene marking factor, which could be a criterion distinguishing the native beef from the foreign one, appeared approximately 1.2 kbp. The native beef was distinguished from the foreign beef with more than 90% of confidence by the gene marking factor. This method was expected to be useful in the breed discrimination between the native beef and the foreign one. The hygienical test results showed that, fortunately, neither Salmonella spp. and Listeria monocytogenes which form a principal cause of the food poisoning nor Enterohemorrhagic Escherichia coli : EHEC which have provoked a recent social disturbance, were detected at all.

Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog

김재훈,홍준배,임범순,조병훈 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

본 연구에서는 DSP (dentln sialoprotein)에서 유래된 합성 펩타이드를 동물실험 모델에 적용하여 치수노출 부위에 서 상아질 재생을 확인하고, 기존 치수복조제와의 성능 비교를 통해 새로운 치수복조제로서의 가능성을 확인하고자 하였다. 6 마리 비글견의 72 개의 치아를 이용하여, 실험적으로 치수를 노출하고 직접 치수 복조술을 시행하였다. 사용한 치수복조제는 (1) Ca(OH)₂ (CH군) (2) DSP 유도 합성 펩타이드 (PEP군) (3) 합성 펩타이드와 Ca(OH)₂ 혼합제(PEP+CH군) (4) White MTA (WMTA군) 이다. 노출된 치수에 치수복조제를 적용한 후 와동은 강화형 글라스 아이오노머로 충전하였다. 시술 후 2 주, 1 개월 및 3개월에 각각 2 마리씩 비글견을 희생시키고 조직시편을 제작하였다. 시편은 H&E 염색 후 광학 현미경으로 치수 염증 반응과 경조직 형성 정도를 관찰하였다. PEP군에서는 17 개의 시편 중 3 개의 시편에서만 경조직 형성을 관찰할 수 있었으며, 대부분의 시편에서 적절한 치수 회복을 관찰할 수 없었다. PEP군은 CH군에 비해 심한 염증반응을 보이고, 경조직 형성은 불량하였다. CH군과 WMTA군은 기계적으로 노출된 치수에서의 염증반응과 경조직 형성에 있어서 유사한 결과를 보였다. The purpose of this study was to investigate the pulpal response to direct pulp capping with dentin sialoprotein (DSP) -derived synthetic peptide in teeth of dogs and to compare its efficacy to capping substances Ca(OH)₂ and white mineral trioxide aggregate (WMTA). A total of 72 teeth of 6 healthy male beagle dogs were used. The mechanically exposed pulps were capped with one of the following: (1) DSP-derived synthetic peptide (PEP group): (2) Ca(OH)₂ (CH group): (3) a mixture paste of peptide and Ca(OH)₂ (PEP+CH group): or (4) white MTA (WMTA group). The access cavity was restored with a reinforced glass ionomer cement. Two dogs were sacrificed at each pre-determined intervals (2 weeks, 1 month and 3 months). After the specimens were prepared for standard histological processing sections were stained with hematoxylin and eosin. Under a light microscope inflammatory response and hard tissue formation were evaluated in a blind manner by 2 observers. In the PEP group only 3 of 17 specimens showed hard tissue formation indication that the DSP-derived synthetic peptide did not induce proper healing of the pulp. Compared with the CH group the PEP group demonstrated an increased inflammatory response and poor hard tissue formation. The CH and WMTA groups showed similar results for di rect pulp capping inmechanically exposed teeth of dogs.