떡 재료 식물인 떡쑥, 모시, 쑥 및 절굿대 추출물의 생리활성 효과

한효심,허북구 한국지역사회생활과학회 2023 한국지역사회생활과학회지 Vol.34 No.1

This study investigated the physiological activity of cottonweed, ramie, mugwort, and globe thistle as material for making Tteok and determined the total phenolic compound and flavonoid contents, and the DPPH and nitrite radical scavenging activities using different extraction methods (hot water, ethanol, and methanol). The amount of total phenolic compounds in the mugwort material increased from 61.0-214.2 mg/L at a concentration of 1,000 mg/L irrespective of the standard substance and extraction method. The total content of flavonoids was the highest in mugwort compared to the other Tteok materials and the extraction methods of hot water, methanol, and ethanol resulted in 29.1, 17.2, and 13.8 mg/L flavonoid content, respectively. At the extracting concentration of 1,000 mg/L, the DPPH radical scavenging activity of the extracts from globe thistle, cotton weed, and mugwort was not significantly different at 76.7-77.0 mg/L, while ramie showed the lowest activity at 70.7 mg/L. When the extracting concentration was 1,000 mg/L, the nitrite radical scavenging activity of hot water and ethanol mugwort extracts increased the most by 25.8% and 29.0%, respectively. However, that of the methanol extract of globe thistle decreased by 21.0%. These results suggest that making Tteok by use may provide useful information.

PCR을 통한 토양에서 Pseudomonas syringae pv. Actinidiae의 검출

한효심,고영진,정재성 한국식물병리학회 2004 식물병연구 Vol.10 No.4

Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.

조의용 화환에 사용된 화훼 종류와 양에 대한 분석

한효심,허북구 한국화예디자인학회 2023 한국화예디자인학 연구 Vol.48 No.-

This study was conducted to secure data necessary for the development of a wreath for condolence, its distribution improvement, consumption and demand forecasting of related cut flower. We have also analyzed the wreaths placed in funeral halls in Gwangju, South Jeollanam-do and Jeollabuk-do provinces in 2000, 2010 and 2022. The percentage of wreaths used in a chrysanthemum flower and artificial palm leaves was 100%. 97.5-100% of the wreaths in combination with artificial lilies were used, and the ratio of artificial dracena was 91.66-100% in the first and second stages of the wreath, but 0% in the third stage. The total number of chrysanthemum flowers used per a wreath for condolence were 118.9(Gwangju)-130.6(Jeonbuk) in 2000, 85.8(Gwangju)-91.3(Jeonbuk) in 2010, and 66.3(Jeonbuk)-73.6(Gwangju) in 2022. More than 73.3% of the wreaths for condolence were used with the artificial lily flowers in yellow, and 7.82-9.0 lily flowers were used per wreath. The number of artificial dracena stems used in the wreath for condolence were 1.85-1.97. The number of artificial palm leaves used in the wreath for condolence were 8.69-9.47. Those results of the clear investigation for the flowers used in the wreath are considered to be useful in determining the consumption and demand of flowers related to the transformation of the wreath. 조의용 화환의 개발과 유통 및 관련 절화의 소비와 수요 예측에 필요한 자료 확보 차원에서 2000년, 2010년, 2022년에 광주, 전남, 전북지역의 장례식장에 배치된 조의용 화환에 사용된 화훼에 대해 조사했다. 일륜국과 플라스틱 종려잎이 사용된 화환의 비율은 100%였다. 조화(造花) 나리가 사용된 조의용 화환은 97.5-100.0%였으며, 조화 드라세나는 화환의 1, 2단에서는 91.66-100.0%가 사용되었으나 3단에서는 0%였다. 2000, 2010, 2022년에 광주, 전남 및 전북지역 장례식장에서 사용된 근조화환 1개당 사용된 일균국의 총수는 2000년의 경우 118.9개(광주)-130.6개(전북)이었으며, 2010년에는 85.8개(광주)-91.3개(전북)였고, 2022년에는 66.3개(전북)-73.6개(광주)였다. 노란색 조화 나리가 사용된 화환은 73.3% 이상이었으며, 화환 1개당 7.82-9.0개의 조화 나리가 사용되었다. 근조화환에 사용된 조화 드라세나 줄기의 수는 1.85-1.97개였다. 근조화환에 사용된 조화 종려잎 수는 8.69-9.47개였다. 이와같이 근조용 화환에 사용된 화훼가 명확하게 조사된 결과는 화환의 변천과 관련 화훼의 소비량 파악 및 수요 예측을 하는데 유용한 자료가 될 것으로 판단된다.

건조화 제조의 학술 연구 동향과 내용 분석에 관한 연구

한효심,허북구 한국화예디자인학회 2023 한국화예디자인학 연구 Vol.49 No.-

This study was conducted to provide the data that easy utilization of existing research results in the industrial sites for a research of dry flower efficiently. We had analyzed research trends and contents on the manufacture of dry flower, focusing on the scientific papers published in the academic journals. According to a survey of 136 papers related the dry flower, the publication period increased in the order of 45.49%(62 papers) from 2001 to 2010 > 33.82%(46 papers) from 2011 to 2020 > 13.97%(19 papers) from 1991 to 2000 > 17.35%(10 papers) after 2021 > 1.47%(2 papers) before 1990. By the manufacturing field of dried flowers, there were much more following the burial drying of flowers by 24.26%(33 papers) > pre-drying by 12.5%(17 papers) > microwave drying by 10.29%(14 papers) > drying of hot air with oven by 8.82%(12 papers) > bleaching by 8.09%(11 papers) > comparison by drying method by 7.35%(10 papers) > freeze drying by 6.62%(9 papers) > the others. We had extracted the title and key contents of each paper and presented them separately by similar methods. Therefore, the results presented by classifying drying methods in this study are expected to help research on drying and select efficient manufacturing methods and directions at industrial sites.

Gentamicin 저항성 R 플라스미드에 존재하는 aacC2 유전자의 상류부위에서 Tn3의 출현

한효심,김남덕,이영종,이효연,정재성 한국미생물학회 1997 미생물학회지 Vol.33 No.3

병원하수에서 분리한 gentamicin 저항성 세균으로부터 aacC2 유전자를 가지고 있는 R 플라스미드 pGM5와 pGM6를 선발하였다. 이들 플라스미드에서 gentamicin 저항성 유전자를 포함하는 부분을 pUC18의 BamHI 자리에 클로닝하여 재조합 플라스미드 pSY5와 pSY6를 각각 얻었다. 재조합 플라스미드의 삽입된 부분에 대한 제한효소 지도를 통해 Tn3 염기서열이 aacC2 유전자의 상류부위에 위치하는 것을 알았다. 재조합 플라스미드의 gentamicin에 대한 민감성의 비교를 통해 Tn3의 bla 유전자 부분과 3'역행중복 부위의 염기서열이 gentamicin 저항성 유전자의 발현에 중요한 역할을 담당하고 있었다. Two gentamicin resistance R plasmids, pGM5 and pGM6, containing aacC2 gene were selected from environmental isolates. The gentamicin resistance determinants of R plasmids were cloned into the BamHI site of pUC18. Restriction enzyme map of inserted region of recombinant plasmids, pSYS and pSY6, and PCR results indicated that Tn3 sequence was located upstream of gentamicin resistance gene. Based on the restriction maps and susceptibility tests, it was concluded that the sequence of bla and 3' inverted repeat of Tn3 play a important roles in the expression of gentamicin resistance gene.

Schisandra nigra Max.에서 암그루에 연관된 SCAR 마커의 개발

한효심,정재성,Han, Hyo Shim,Jung, Jae Sung Korean Society of Life Science 2021 생명과학회지 Vol.31 No.6

자웅이주 식물로 알려진 Schisandra nigra Max. (흑오미자)는 우리나라 제주도에 자생하고 있으며, 열매를 얻기 위해 일부 농가에서 재배되고 있다. 열매 생산을 위해서는 수그루에 비해 암그루의 가치가 더 높기 때문에 묘목단계에서 일찍 성별을 아는 것은 중요하다. 이 연구에서는 S. nigra의 유전체에서 암그루에 특이적인 부위에 관련된 SCAR 마커를 개발하였다. 120개의 무작위로 구성된 RAPD 프라이머 중에서 OPB-03 프라이머가 암그루에서 749 bp의 밴드를 안정적으로 증폭시켰다. 암그루 특이적인 PCR 산물을 분리하여 클로닝한 뒤 염기서열을 결정하였다. 이 암그루 특이적인 절편을 탐침으로 사용한 Southern hybridization에서 암그루에서만 양성반응이 나타나고 수그루에서는 잡종화가 일어나지 않았다. 이러한 결과는 749 bp의 DNA 절편이 암그루의 유전체에는 존재하지만, 수그루에는 없음을 시사하였다. RAPD 마커로부터 암그루에서만 436 bp를 증폭시키는 SCAR 프라이머를 설계하였다. 이 프라이머 쌍은 암그루와 자생지에서 수집한 4개의 자웅동주 식물에서만 예상되었던 크기의 DNA 절편을 증폭하였다. 이 연구에서 개발된 SCAR 마커는 묘목 단계에서 암꽃이 피는 개체를 선발하는데 사용될 수 있을 것이다. Schisandra nigra Max., a dioecious plant native to Jeju Island in Korea, is cultivated on a small scale for fruit production. As fruit-producing female individuals are generally considered to be more valuable than male, early identification of plant sex at the seedling stage is important. In this study, a sequence-characterized amplified region (SCAR) marker associated with a female-specific region in the genome of S. nigra was investigated. Of 120 randomly amplified polymorphic DNA (RAPD) primers, one primer (OPB-03) consistently amplified a 749 bp band in female plants. The female-specific PCR product was isolated and cloned, and the nucleotide sequences were then determined. Southern hybridization performed using the female-specific fragment as a probe produced positive signals only in genomic DNA from the female plants. This result revealed that the 749 bp segment of DNA was present in the genome of female plants but absent in the genome of male plants. A SCAR primer pair was designed based on the RAPD marker to amplify a 436 bp fragment in the genomic DNA of female plants. This primer pair amplified the expected size of DNA fragment in female plants and four monoecious individuals collected from a natural population. The SCAR marker identified in this study can be used to distinguish female-flowering individuals at the seedling stage.

병원하수로부터 분리한 Gentamicin 저항성 세균에서 Tn3에 의한 aac(3)II의 발현 증가

한효심,이문숙,정재성 한국미생물학회 2004 미생물학회지 Vol.40 No.1

Gentamicin에 저항성을 나타내는 세균을 병원 하수로부터 분리하여 aminoglycoside-(3)- N-acetyltransferase를 암호화하는 유전자인 aac(3)II의 존재여부를 dot-blot hybridization으로 조사하였다. aac(3)II유전자의 일부를 탐침으로 사용한 결과 gentamicin저항성 세균의 41% (39/95)가 이 유전자를 가지고 있었다. aac(3)II와 Tn3에서 각각 설계된 primer를 사용한 PCR 결과 aac(3)II를 가지고 있는 39개 균주 중 13개 균주가 TnS-aac(3)II구조를 가지고 있음을 알 수 있었다. aac(3)II의 상류에 Tn3를 가지고 있는 13개 균주의 gentamicin에 대한 최소억제농도는 가지고 있지 않은 균주에 비해 상대적으로 높았다. 13개 균주를 동정한 결과 5개는 Eschelichia coli, 3개는 Acinetobacter johnsonii, Enterobacter agglomerans와 Micrococcus luteus가 각각 2개, 그리고 1개의 Pseudomonas facilis로 동정되었다. 이러한 결과들은 Tn3-aac(3)II구조가 gentamicin 저항성 세균들에서 널리 분포하고 있음을 말해준다. We tested gentamicin - resistant bacteria isolated from hospital sewage to confirm the presence of aac(3)II encoding aminoglycoside- (3)-N- acetyltransferase by dot-blot hybridization. A probe from the internal fragment of aac(3)II was hybridized to DNA from 41 % (39/95) of gentamicin resistant isolates. PCR was performed with primers from aac(3)II and Tn3. Of 39 strains, 13 strains had Tn3-aac(3)II structure. Minimal inhibitory concentration (MIC) test demonstrated that 18 strains containing Tn3-aac(3)II showed higher resistance to gentamicin than those of other strains. Thirteen strains were identified as 5 Escherichia coli, 3 Acinetobacter johnsonii, 2 Enterobacter agglomerans, 2 Micrococcus luteus, and 1 Pseudomonas facilis. These results suggest that gentamicin-resistant determinant of Tn3-aac(3)II structure was widely distributed in the gentamicin-resistant bacteria.

토착미생물별 가축분 퇴비화 과정중 생물화학적 특성 변화

한효심(Hyo-Shim Han),이경동(Kyung-Dong Lee) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.6

다양한 기능을 가진 토착미생물을 분리·동정 후 퇴비화에 적용하여 우수한 기능성을 가진 발효퇴비를 제조하고자 하였다. 7개의 우수 토착미생물을 주축으로 3개의 발효퇴비를 제조하여 생리활성을 조사한 결과, 대두의 발아율 증가, 섬유소, 키틴, 단백질 분해 등의 생리활성이 대조구보다 뛰어나고, 오옥신 생산량이 유의적으로 증가하였다. 퇴비중 암모니아 가스와 휘발성 지방산 함량 역시 2차 발효가 시작되는 20일까지 함량 감소가 있었고, 대두의 건물수량이 증가함을 확인하였다. 또한 식물보호제 분해시험에서도 유의적인 분해율이 있었고, 중금속 함량 역시 유의적으로 감소하였다. 따라서 토착미생물의 다 기능적 활성을 활용한 발효퇴비의 제조는 기존보다 더 향상된 발효퇴비를 농업생산 환경에 적용할 수 있을 것이다. In order to produce high-quality fermenting composts, bacteria strains with high activities of extracellular enzymes (cellulase, chitinase, amylase, protease and lipase) were isolated from the soils in 6 provinces of Korea, and characterized by 16S rRNA gene sequence analysis and properties. The selected 7 stains inoculated to livestock manure for 2’ fermenting time, and experimental treatment divided into 3 groups, B1, B2 and B3, according to microbial activity and enzyme type. Our results showed that microbe applications (B1, B2 and B3) can increase (p<0.05) both rhizomes (17-38%) and enzyme activities (50-81%) in compost after fermenting time, respectively, compared to non-microbe treatment (control). The microbe application also decreased significantly (p<0.05) the NH3 and H2S gas contents 13.4 and 27.3% compared with control, and the Propionic acid and Butyric acid gas contents 14.5 and 19.6%, respectively, as compared to the control. The microbial degradation rate (%) of pesticides and heavy metals increased significantly (p<0.05) after fermenting time, respectively, as compared to the control. Especially, microbe applications were more effective in total rhizomes yields and bioactivities than non-microbe treatment. Thus the results of this study could help in development of potential bioinoculants and composting techniques that maybe suitable for crop production, and protectable for earth environment under various conditions.

연구보문 : 식물환경 ; 울금의 생육, 수량 및 품질에 미치는 퇴비의 효과

한효심 ( Hyo Shim Han ),우서 ( Seo Woo ),김동관 ( Dong Kwan Kim ),허북구 ( Buk Gu Heo ),이경동 ( Kyung Dong Lee ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.2

Curcuma longa L. (turmeric) is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout Asia etc. Recently, the demands on rhizome of turmeric are increasing greatly by well-being boom, but there is not enough to meet the demands. To fulfill increasing demands, cultivation system strategies using the organic fertilizers are required to produce a greater amount of rhizome with good quality and yield. A field experiment was conducted to determine the effect of composts, NPK fertilizer(control, F), NPK + swine manure(SM) and NPK + fermentation manure from the wasted oriental medicine materials(OMWM), on rhizome yield and the content of bioactive components for quality. Our results showed that two compost applications can increase both rhizomes(24.1-25.9%) and curcumin(21.7-41.0%) yields, respectively, compared to F control. The content of amino acids increased significantly by SM and OMWM treatments. SM and OMWM application also increased the total phenol yields 7.8 and 8.7 g/10a compared with control 6.3 g/10a, the flavonoid yields 6.3 and 7.3 g/10a compared with control 5.3 g/10a, and also antioxidant activity 21.7 and 41%, respectively, as compared to the control. Especially, OMWM was more effective in total rhizomes yields and bioactivities and in the biosynthesis of curcumin and bioactive components than SM treatments, but the biological pathway was not clear, still. This experiment suggests that curcumin or bioactive components affected by adding SM and OMWM could increase the yields and quality of turmeric.

전라남도 동부지역 오일장의 특산인 ‘국거리’ 상품에 관한 연구

한효심(Hyo Shim Han),조자용(Ja Yong Cho),허북구(Buk Gu Heo) 한국농어촌관광학회 2023 농어촌관광연구 Vol.26 No.1