The effects of cervicovaginal stimulation and administration of naloxone on the secretion of prolactin in the rats.

한세준(SJ Han),오세량(SR Oh) 대한산부인과학회 1989 Obstetrics & Gynecology Science Vol.32 No.9

Serum prolactin levels were measured following decapitation of the adult female rats, which were stimulated cervicovaginally with a cotton rod or injected with naloxone into the abdominal cavity or both just before the onset of study and 1 hour, 5 hours, 7 hours and 9 hours later. The afternoon prolactin surge in the normal rats was most prominent at 16:30 reaching 60.90±10.72 ng/ml, whereas in the cervicovaginally sitmulated rats, the plasma prolcatin levels showed a peak at 14:30 and was significantly higher than the normal afternoon prolactic surge level. In the naloxone-injected rats, the plasma prolactin level did not increase and in the cervicovaginally stimulated rats with simultaneous injection of naloxone , neither the prolactin surge nor decrease in plasma prolactin level were observe.

Dehydroepiandrosterone Sulfate 투여가 산욕기부인의 유즙분비에 미치는 영향

홍순묵(SM Hong),정종일(JI Jung),김영철(YC Kim),한세준(SJ Han),오세량(SR Oh) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.6

The effect of Dehydroepiandrosterone sulfate (DHEA-S) which is clinically used for the ripening of uteirne cervix during delivery, was given to the laboring women to determine the adverse effect on lactation during the 48 hours after delivery. The women who were given DHEA-S showed a significant decrease of lactation in comparisen with the women without it. So, the above result suggests that the administration of DHEA-S is useful to the ripening of the uterine cervix, however it is converted to estradiol and inhibits lactation at least for 48 hours after delivery.

난소임신 2 례

문경란(KL Moon),손대언(DY Soon),박일웅(IW Park),김구현(GH Kim),정혁(H Jung),송창훈(CH Song),한세준(SJ Han),김윤신(YS Kim) 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.3

본 저자들은 Spiegelberg`s Criteria(1878)를 만족시키는 난소임신을 가능한 정상 난소조직을 보존시킬 목적으로 난소 wedge절제술과 낭종절제를 시행하여 병리조직학적으로 확진된 난소임신 2례를 경험하였기에 문헌적 고찰과 함께 보고하는 바이다. Ovarian pregnancy is very rare of ectopic pregnancy. We have experienced two cases of ovarian pregnancy, which are presented with a brief review of the literature.

난소의 양성기형종에서 발생한 소세포암의 좌측 견갑상부임파선 원격전이 1 례

한세준,최상준,류경철 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.2

The development of cancer in a pre-existing benign cystic teratoma of the ovary with distant metastasis to the supraclavicular lymph nodes has not been previously reported. We experienced a case of a 28-year-old woman with distant metastasis from small cell cacinoma arising in benign mature cystic teratoma of the ovary to the supraclavicular lymph nodes. The staging laparotomy and total abdominal hysterectomy with both salpingooophorectomy were peformed. There was no metastatic site except the supraclavicular lymph nodes, thereafter adjuvant chemotherapies with bleomycin, etoposide, cisplatin have been doing. We report a case of benign cystic teratoma with malignant change and metastasis with the literature.

외과적수술에서 Naloxone이 혈장 Prolactin치 및 LH치의 변동에 미치는 영향

한세준,오승진,송창훈,박석현,나윤정,김왕수,송경언,황순철 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.8

각종 대, 소수술과 NAL 투여에 따른 혈장 PRL치와 LH치의 변동상황을 알기 위하여 남성 10명, 여성 16녕 도합 26명을 대상으로 하여 방사면역측정용 kit로서 이들 hormone치를 측정하였다. 그 결과, 1. 수술환자의 혈청 PRL치는 수술시작전 62.31±26.55ng/ml에서 수술시작후 15분에서 203.42±52.50ng/ml로 크게 증가하고(P$lt;0.01) 수술시작후 45분에서는 별반 변동없이 207.76±57.93ng/ml를 보였다. 2. 대수술의 경우에는 혈장 PRL치가 수술시작후 15분에 115.40±25.42ng/ml로 증가하였고 수술시작후 45분에서도 계속 증가하여 206.20±107.23ng/ml로서 대조군의 수치에 도달하였다. 3. 소수술의 경우에는 혈장 PRL치가 수술시작후 15분에서 최고치인 257.81±117.23ng/ml를 보였다가 수술시작후 45분에서는 226.18±68.33ng/ml로 감소하기 시작하였다. 4. NAL을 투여한 경우에는 혈장 PRL치가 수술시작후 15분과 45분에서 각각 186.93±71.94ng/ml와 111.53±24.52ng/ml로서 15분후에 일단 peak에 도달하였다가 감소하기 시작하였다. 5. NAL을 투여한 경우에는 혈장 PRL치가 15분후에서 228.63±78.88ng/ml이었고 45분후에서는 364.36±121.58ng/ml로서 계속 증가하여 45분후에 peak에 도달하였다.(P$lt;0.05). 6. 수술환자의 혈청 LH치는 수술전에 52.52±37.71ng/ml이었던 것이 15분후와 45분후에 각 130.39±33.43ng/ml ,133.22±34.53ng/ml로서 15분치의 LH치가 45분후까지 그대로 유지되었다. 7. 대수술의 경우에서는 혈창 LH치가 15분후와 45분후에 각각 51.29±41.04ng/ml, 43.47±34.15ng/ml로서 증가하지 않아 수술전치와 별반 변동이 없었다. 8. 소수술의 경우에는 15분후에 179.83±44.41ng/ml. 45분후에 189.31±47.38ng/ml로서 계속적으로 증가하였다. (P$lt;0.01). 9. NAL을 투여하지 않으면 혈장 LH치가 15분후에 83.86±31.38ng/ml, 45분후에 103.14±41.41ng/ml로 점차 증가하며 NAL을 투여하면 혈장 LH치가 15분후에 193.85±63.45ng/ml에 달하고 45분후에는 174.24±58.98ng/ml로 감소하여 혈장 LH치는 NAL에 의하여 증가하였다. The concentrations of plasma PRL were increased at 15 minutes and 45 minutes after surgery. In the minor surgery the plasma PRL levels were more significantly increased, than those in the major surgery. In the administration of Naloxone Chloride or not, the latter levels of plasma PRL more significantly increased comparing to those of the former levels. The concentrations of plasma LH did not change at 15minutes and 45minutes after surgery comparing to presurgery levels. In the major surgery, the plasma LH levels did not increased, but in the minor surgery at the time of 15 minutes and 45 minutes after surgery plasma LH levels were significantly increased. At the administration of NAL, plasma LH levels were significantly increased than those without administration. We conclude that the plasma PRL and LH levels may be more significantly increased in the minor surgery at administration of NAL. However, those mechanisms are obscure.

자궁경부암 조기 검진에 있어서 내원 환자를 대상으로 한 삼중병합검진의 임상적 평가

한세준,조상무,노형균 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.3

Objective; The purpose of this study was to compare cervical cytology[Pap smear], HPV DNA test, cervicography, first double combined test of cervical cytology and HPV DNA test, second double combined test of cervical cytology and cervicography and triple combined test of cervical cytology, HPV DNA test and cervicography. Study design; This study included 261 patients who visited for cervical cancer screening at the department of Obstetrics and Gynecology, Chosun university hospital from Dec. 1, 1995 to Nov. 30, 1996. All women simultaneously underwent cervical cytology, HPV DNA test and cervicography for cervical cancer screening and colposcopically directed biopsy for diagnostic evaluation. Results; The triple combined test[cervical cytology + cervicography + HPV DNA test; 100%] was most sensitive among the cervical screening tests[100%]. Second double combined test[cervical cytology + cervicography; 98.1%] was more sensitive than first double combined test[cervical cytology + HPV DNA test; 92.3%]. But cervical cytology was most specific[93.5%] and showed the highest positive predictive value[77.8%]. Conclusions; The sensitivity of cervical cytology was markedly improved by combination with HPV DNA test and cervicography. So the triple combined test which improve high false negativity of cervical cytology may be a new effective tool as a cervical cancer screening test, if the effectiveness is confirmed by mass screening study.

양막대증후군 1 예

한세준,송창훈,최종범 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.11

저자들은 임신 33주에 초음파와 KUB상에 무뇌증이 발견되고, 초기파막 및 조기진통에 의해 사산된 태아에서 두부, 안면 및 사지를 침범하는 다발성 선천성기형을 관찰하였다. 이 중 두부기형은 무뇌증을 동반한 encephalocele이었으며, 안면부기형은 cleft palate, cleft lip 및 상악골분리, 코 및 눈의 형성 부전 등이었고 결손된 안면부 및 두부에 양막이 유착되어 있었기에 본 증례가 선천성 양막대증후군에 속한다고 생각하고 본 예를 기술하였다. 양막부착은 사지 결손과도 관계가 있었다. Congenital amniotic band is a rare and interesting anomaly that may attach to various parts of the fetus or newborn, and can be cause of variety of congenital malformations. The amniotic band syndrome implies a group of amomalies resulted from the compression-related defects including from oligohydroamnios, and resulted from band or adhesionrelated defect. We reported an case of a amniotic band syndrome revealing anencephaly, meningoencephalocele, cleft palate, claft lip, non-union of maxilla, amputation of the right tumbtip, left and right great toes, clup foot. The amniotic membrane of the placenta was attached to the anencephalic head and the face. This stillborn female baby was induced at 33 weeks of gestation because of spontaneous premature rupture of membrane and ancephaly detected on ultrasonography. The 23-year-old primigravida mother had no history of taking drugs or irradiation during this pregnancy.

임신성고혈압 환자의 태반에서 Glutathione량의 변화에 관한 연구

한세준,김왕수 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.9

Glutathione has a key role in several detoxification reactions and in the protection against injury caused by reactive oxygens. Pregnancy-induced hypertension(PIH) is associated with endothelial cell dysfunction. Such dysfunction could be caused by oxidative stress. There is evidence of increased activity of free radicals in PIH, but little is known about the part played by changes in specific antioxidants. In this study, the changes of glutathione levels were investigated in blood of patients with PIH, and cord blood of these patients was also investigated. The glutathione levels in cord blood of neonates from hypertensive pregnant women were significantly higher than in cord blood from normotensive pregnant women. The changes of γ-glutamuylcysteine synthetase and of glutathione s-transferase in placenta were not significant, but γ-glutamyl transpeptidase activity increased significantly in placenta of hypertensive pregnant women. These results suggest that the increased glutathione in cord blood of patients with PIH may be due to increased glutathione interorgan transport resulting from incerased activity of the placenta γ-glutamyl transpeptidase.

Alloxan 유도 당뇨임신백서와 그 태아 및 신생쥐의 폐에서 SOD , Catalasse 및 GPX 활성의 변화에 관한 연구

한세준,홍관표,배학연 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.1

The activites of pulmonary antioxidant enzymes in alloxan-induced diabetic pregnant rats. their fetuses and neonates. The superoxide dismutase(SOD) and catalase activity were increased in diabetic nonpregnant female rats in comparison with normal control female rats. But the chagnes of antioxidant enzymes in lung of diabetic pregnant rats were not significant. In fetal lung of diabetic rats, the catalase activity decreased significantly, where as the changes of SOD and glutathione peroxidase(GPX) activity were not siginificant in compared with nondiabetic fetal lung. The SOD activities in lung of neonates from diabetic female decreased at day 1 and days 7. In lungs, the catatlase activities decreased significantly at day 1, days 7 and days 14 after birth. In these results decreased activities of catalase and SOD were more susceptible to oxidative lung injury, and these alterations of antioxidant enzymes may be a causative factors of respiratory distress in diabetic neonates.

부인과 종양과 면역학

한세준 대한산부인과학회 1991 Obstetrics & Gynecology Science Vol.34 No.2

초기 면역학적 측면에서 종양의 진단과 치료는 매우 낙관적이었으나 현재까지 이루어진 성과는 매우 미약하다. 암표지물은 암조직에서 특이적으로 생산되는 물질로서 종양의 진단, 분류 및 치료에 대한 반응 등을 알 수 있는 지표로 이용 될 수 있는데, 현재까지 발견된 수많은 암표지물질 중 극히 일부만 임상에서 환자에 이용되고 있는 실정이고, 종양을 진단하기 위해서는 특정종양에만 특이성이 있는 암표지물질만이 유용하게 이용될 수 있기 때문에 확실한 tumor associated antigens을 찾기 위한 영구가 계속되고 있다. 그러나 임의 staging에 따른 분류나 치료에 대한 반응을 감지하는데는 암표지물질이 현재에도 임상에서 대단히 유용하게 이용되고 있다. 대부분의 과학자들은 종양에 대한 면역학적 치료에 대해서 회의적으로 생각하고 있는데, 이는 면역요법에 대한 과학적인 지식의 부죽때문이다. 최근 동물실험에서 성공한 암에 대한 면역요법을 사람에서는 성공시키지 못한 경우도 있는데, 이는 암에대한 면역요법은 매우 정교한 조건을 갖추어야 하는데 실험동물에서는 이러한 조건을 인위적으로 조절이 가능하지만 사람에게 적용은 매우 어렵기 때문으로 사료된다. 또한 사람에서 원발성 국소종양의 치료는 수술적인 방법에 의해서 완치가 되기때문에 수술적 방법으로 치료하며, 전이암의 경우 수술적 방법으로서 치료가 불가하기때문에 방사선 요법이나 면역요법을 시행하기 때문에 치료효과가 떨어지고, 특히 면역원성이 더 낮은 종양이 전이가 더 잘되므로 전이암에 대한 면역요법은 효과가 적다. 따라서 현재 시행되고 있는 개체내에 이미 성숙되어 있는 면역계를 이용하는 방법이 아닌 숙주의 면역감시기능으로부터 벗어난 암세포를 제거할 수 있는 새로운 방법의 개발이 절실히 요구된다.