http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
NaNO<sub>3</sub>, NaHCO<sub>3</sub> 농도가 Arthrospira platensis 생장에 미치는 영향
최수정,하종명,이재화,Choi, Soo-Jeong,Ha, Jong-Myung,Lee, Jae-Hwa 한국생물공학회 2015 KSBB Journal Vol.30 No.6
Arthrospira platensis (A. platensis) is one of the most explored cyanobacteria and has been studied for proteins, vitamins, pigment (chlorophyll and carotenoids) and fatty acid. In this study, we tested the effect of $NaHCO_3$ and $NaNO_3$ on the microalgae growth under photoautothrophic culture in A. platensis. As a result, cell growth and dry cell weight were increased in proportion to the $NaHCO_3$ and $NaNO_3$ concentration. Pigment (chlorophyll and carotenoids) contents of A. platensis were increased with proportion to $NaHCO_3$ concentration. But, the content of pigment (chlorophyll and carotenoids) in 100% $NaNO_3$ medium of A. platensis was the highest, 40%, 140% and 200% $NaNO_3$ medium with pigment content of A. platensis was reduced. In conditions of $NaHCO_3$ (50%) or $NaNO_3$ (40%) limitation, A. platensis could accumulate lipids to high as 1.7-fold and 1.3-fold.
항산화능이 증대된 Arthrospira platensis 균주 개량 및 특성 분석
최수정(Soo-Jeong Choi),이재화(Jae-Hwa Lee) 한국생물공학회 2015 KSBB Journal Vol.30 No.3
Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant ‘1-9’, ‘2-5’, and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.
지질 고 생산성 Chlorella vulgaris 변이주 분리 및 특성 분석
최수정 ( Soo Jeong Choi ),박현진 ( Hyun Jin Park ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2015 공업화학 Vol.26 No.5
Micro-algae Chlorella vulgaris (C. vulgaris) is an important source for bio-diesel because of the high content of neutral lipids. In this study, we intended to induce mutants of C. vulgaris by UV-B irradiation. C. vulgaris was first exposed to UV-B for 1, 2, 3, 4, 5 min. As the UV-B exposure time increased, the cell viability and pigment content were decreased. Mutants of C. vulgaris were also induced through ultraviolet irradiation and two strains were selected with respect to lipid contents, where were named as ‘UM10’, ‘UM15’. They were then cultivated in the same way as to the wild type. After 21 days of cultivation, the cell growth, dry cell weight, pigment contents, and lipid contents were measured for investigating characteristics of mutants. As a result, the cell growth and dry cell weight of both mutants increased about 1.4 and 1.5 times, respectively compared with those of wild type. In addition, chlorophyll and carotenoid contents were measured in order to investigate pigment contents in micro-algae through photosynthesis. It was shown that chlorophyll and carotenoid contents of both mutants decreased about 10% compared to those of wild type. Lipid contents in UM10 and UM15 increased about 1.2 and 1.5 times, respectively compared to that of wild type.
산 및 효소 가수분해를 이용한 홍조류로부터 바이오 에탄올 생산
최수정 ( Soo Jeong Choi ),이성목 ( Sung Mok Lee ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2012 공업화학 Vol.23 No.3
화석연료로 인한 환경오염 등의 문제를 해결하기 위해서 다양한 원료를 이용하여 바이오 에탄올 생산에 대한 연구가 진행되고 있다. 해조류 중에 홍조류는 agar, carrageenan, porphyran으로 구성되어 있어 산 처리를 통해 바이오에탄올 생산에 유용한 바이오매스로 전환이 가능하다. 본 연구는 홍조류의 가수분해물을 이용하여 바이오에탄올 생산의 최적 조건을 찾으려고 한다. 바이오에탄올 생산하기 위해 전처리 된 우뭇가사리에 Saccharomyces cerevisiae KCCM112를 접 종해 발효하였다. 우뭇가사리 가수분해의 최적조건은 1.5% H2SO4를 121 ℃에서 30 min 반응시켰을 때 7.04 g/L의 galactose와 1.94 g/L의 glucose가 생산되었다. 그리고 CH3COOH의 경우 2.0% 농도로 처리하였을 때, galactose 0.75 g/L가 생산되었다. 이와 반대로 도박에서는 H2SO4 1.5%를 처리하였을 때 galactose를 6.38 g/L 생산하였으며, CH3COOH을 처리했을 때 0.368 g/L이 생산되었다. 우뭇가사리에서 에탄올 생산은 1.0% H2SO4를 121 ℃에서 30 min 간 처리하였을 때 가장 높았으며, 96 h 배양하였을 때 3.77 g/L의 에탄올을 생산했다. Bio-ethanol production research using various material has been problemed for solving problems of environment pollution caused by fossil fuels, Red-algae consists of agar, carrageenan, and porphyran, if it is treated by acid, it is able to change useful bio-mass for bio-ethanol. In this study, we found an optimal condition for bio-ethanol production from acid hydrolysate in red-algae. To produce bio-ethanol, Saccharomyces cerevisiae KCCM1129 inoculated to acid hydrolysate of Gelidium amansii. The optimal condition for Gelidium amansii hydrolysis was found to be 30 min reaction at H2SO4 concentration of 1.5% and 121 ℃, At this condition, its produced to 7.04 g/L galactose and 1.94 g/L glucose. And acetic acid concentration of 2.0% in agar produced 0.75 g/L galactose. In contrast, Pachymeniopis elliptica was treated with H2SO4 concentration of 1.5%, it produced 6.38 g/L galactose. And Pachymeniopis elliptica treated with acetic acid concentration of 2% produced 0.368g/L galactose. The optimal condition of ethanol production was found to be 96 h reaction at H2SO4 concentration of 1.0% and 30 ℃, which produced 3.77 g/L ethanol.
신장 ; 혈액투석 환자에게서 Biohole(TM) 쐐기를 이용한 Buttonhole 천자법의 임상적 유용성
최수정 ( Soo Jeong Choi ),이설형 ( Seol Hyoung Lee ),조은희 ( Eun Hee Cho ),오혜란 ( Hye Ran Oh ),김은정 ( Eun Jung Kim ),박무용 ( Moo Yong Park ),김진국 ( Jin Kuk Kim ),황승덕 ( Seung Duk Hwang ) 대한내과학회 2012 대한내과학회지 Vol.82 No.3
Background/Aims: Buttonhole cannulation has been popular because it provides an easy puncture, is less painful, and requires less time for hemostasis. However, the technique requires a skilled staff and a long time to form the tract. A new buttonhole technique using Biohole(TM), which shortens the time needed for tract formation, has been introduced in Europe, North America, and Japan. Methods: We prepared a cannulation tract using the buttonhole technique and Biohole(TM) over a two-week period and compared the 12-week outcomes between patients who underwent the rope-ladder versus buttonhole techniques. Results: The 40 patients (27 males) had a mean age of 49.1±14.2 years. Thirteen and 27 patients were cannulated with the rope-ladder and buttonhole techniques, respectively. Patients who underwent the buttonhole technique had more initial pain than did those who received the rope-ladder technique (p=0.044). The Biohole(TM) procedure improved puncture pain (5.6 vs. 3.4, p=0.003) and shortened hemostasis time (1.8 vs. 1.3, p=0.001). Over a two-week period, patients using Biohole(TM) experienced dislocation (20.8%), bleeding at peg sites (8.6%), and pain during peg change (2.4%). Over the 12 week study period, patients who underwent the buttonhole technique had insignificantly less pain than did those who received the rope-ladder technique (p=0.088), but the former had less bleeding time than the latter (p=0.000). One patient who received the buttonhole technique experienced one episode of infection (p=0.327). Conclusions: The new buttonhole technique using Biohole(TM) is safe and useful in the short term. A long-term, larger, multicenter study is required to confirm these results. (Korean J Med 2012;82:307-312)
NaNO₃, NaHCO₃ 농도가 Arthrospira platensis 생장에 미치는 영향
최수정(Soo-Jeong Choi),하종명(Jong-Myung Ha),이재화(Jae-Hwa Lee) 한국생물공학회 2015 KSBB Journal Vol.30 No.6
Arthrospira platensis (A. platensis) is one of the most explored cyanobacteria and has been studied for proteins, vitamins, pigment (chlorophyll and carotenoids) and fatty acid. In this study, we tested the effect of NaHCO₃ and NaNO₃ on the microalgae growth under photoautothrophic culture in A. platensis. As a result, cell growth and dry cell weight were increased in proportion to the NaHCO₃ and NaNO₃ concentration. Pigment (chlorophyll and carotenoids) contents of A. platensis were increased with proportion to NaHCO₃ concentration. But, the content of pigment (chlorophyll and carotenoids) in 100% NaNO₃ medium of A. platensis was the highest, 40%, 140% and 200% NaNO₃ medium with pigment content of A. platensis was reduced. In conditions of NaHCO₃ (50%) or NaNO₃ (40%) limitation, A. platensis could accumulate lipids to high as 1.7-fold and 1.3-fold.
전자빔 조사를 통해 유도된 Arthrospira platensis 변이주 분리 및 특성 분석
최수정 ( Soo Jeong Choi ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2015 공업화학 Vol.26 No.5
Arthrospira platensis has high lipid and pigment (such as chlorophyll and carotenoid) contents and thus evaluated as an important resource in functional food production. The cell growth rate and pigment concentration of EM24 increased by approximately 1.2-fold than those of the wild-type strain (WT). Fluorescence intensity levels in EM24, which were quantified with a lipid triolein standard curve, also increased by approximately 1.5-fold than those in WT (62.9 mg/Lvs. 38.9 mg/L). The analysis of fatty acid profiles indicated that the gamma-linoleic acid level in EM24 increased by 1.5-fold than that in WT.