포스터 발표 초록 ; Poster Ⅰ : PART A : Proteins - Structure , Functions & Modifications ; Branched chain 아미노산과 대사 산물들이 Serratia marcescens acetolactate synthase 의 생합성에 미치는 영향

최병범,김승수 한국생화학분자생물학회 (구 한국생화학회) 1991 추계학술대회집 Vol.- No.-

Branched chain 아미노산과 대사 산물들이 Serratia marcescens acetolactate synthase 의 생합성에 미치는 영향

최병범,김승수 생화학분자생물학회 1991 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

대학생의 혼자식사와 가공식품에 대한 인식 및 섭취 실태 조사

최병범,Choi, Byung Bum 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.6

This study was conducted to assess the recognition and consumption of meal alone and processed food according to major of college students in Seoul Metropolitan area and Chungcheong province, Republic of Korea. To accomplish this, a survey was conducted to investigate 353 college students (171 food & nutrition majors and 182 non-majors) regarding their general characteristics, dietary behaviors, meal alone issues, and the purchase and consumption of processed food. Most major and non-major students skipped breakfast. The main reason for skipping was not having time. Majors and non-majors put great importance on their lunch and evening meal, respectively. Nutrition information routes for majors were 'college class' (62.5%) and 'TV radio' (17.5%). However, the routes for non-majors to obtain nutrition information were 'internet smart phones' (41.1%) and 'TV radio' (28.3%). These results suggest the need to provide correct information concerning nutrition to college students. Most major and non-major students tend to have 0 to 2 times of meal alone per week. The main reason for meal alone was schedule unsuitable for eating with family or friends. The most critical factors for majors and non-majors when they chose menu to have meal alone were personal taste and money, respectively. The primary consideration for choosing processed food was price and the main purchase route was convenience store. The main reason for consuming processed food was easy-to-cook. Of processed foods, the most favored ones were noodles and fish products. Meal alone frequency was positively correlated with age (p<0.05), grade (p<0.05) and monthly allowance (p<0.05). Preference and consumption frequency of processed food were negatively correlated with concern about nutrition (p<0.05) and meal frequency (p<0.05), respectively. Based on these results, greater efforts should be made to provide meaningful information regarding programs for nutrition education to improve their dietary habits. Research and development of processed food specifically for college students eating alone should be increased.

쑥의 생리활성 성분 연구

최병범,이혜정,방선권 한국식품영양학회 2005 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2005 No.06

중부지역 대학생의 가공식품 이용실태에 관한 연구

최병범 신한대학교 2020 신한대학교 논문집 Vol.43 No.

The purpose of this study was to research the use status of processed food according to the grade of college students. For this study, 353 college students(101 freshman(28.6%), 104 sophomore(29.4%), 92 junior(26.0%), 56 senior(15.8%)) in the Seoul Metropolitan area and Chungcheong province was surveyed by the questions regarding their general characteristics, the purchase and consumption of processed food. In all grades, the largest percentage of monthly allowance was ‘200,000 to 300,000 won’(30.7, 36.5, 37.0, 35.7%), with more than half of the food expense rate on allowance accounting for 44.2%. Currently, the most stressful part is ‘academy’(36.2, 36.4, 26.1%) in the freshman to junior and ‘employment’(39.6%) in the senior, while friends are the most likely to discuss stress together. Preference for processed foods was generally favorable in all grades and advertising routes for processed foods were mostly ‘TV·radio’·‘sale store’ and the first consideration in purchasing processed foods was the ‘price’. The consumption frequency of processed foods was ‘1 to 3 times per week’(41.6, 38.5, 48.9, 41.1%) and ‘4 to 6 times per week’(29.7, 28.8, 29.4, 35.6%) in the freshman to senior, respectively. The primary reason for consumption of processed foods was ‘easy to cook’ and the most favored food was ‘noodles’ in all grades. The results suggest that college students' food education and programs to improve dietary behavior on processed foods are necessary, and could be provided as basic data for the food industry.

이소루신 생합성 과정에서 Serratia marcescens Threonine Dehydratase의 조절 역할

최병범,방선권,김승수 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4

최소 배지에 여러 아미노산을 첨가하여 배양시킨 Serratia marcescens ATCC 25419 세포 추출물에서 threonine dehydratase의 비활성을 조사한 결과 이소루신은 1∼4mM에서 효소의 비활성을 100∼140% 정도 증가시킨 반면 15∼30mM에서 25∼80% 정도 감소시켰다. 루신은 효소의 비활성을 1∼20mM에서 60%이상 감소시켰다. 한편, 발린은 효소의 비활성을 1∼4mM에서 20% 정도, 8∼10mM에서 10%정도 감소시켰으나 20mM에서 효소의 비활성을 원래대로 회복시켰다. 각 2mM 농도의 이소루신과 발린 그리고 이소루신과 루신은 효소의 비활성을 각각 75, 50%씩 증가시킨 반면 세 아미노산을 각각 2과 10mM씩 동시 첨가했을 경우에는 각각 25, 58%씩 감소시켰다. 글리옥실산은 효소의 비활성을 75%, 이마다졸, 피루브산, α-아미노부티르산 그리고 AMP는 모두 50%씩, 호모세린, α-케토글루타르산, α-케토부티르산 및 IPTG를 첨가했을 경우는 모두 25%씩 감소시켰다. cAMP는 표소의 비활성을 1∼2mM에서 10∼40%정도 감소시킨 반면 10mM에서 80% 증가시켰다. 이러한 결과들로부터 S. marcescens threonine dehydratase는 E. coli K-12 효소와는 다르게 낮은 농도의 이소루신에 의해 효소의 비활성이 증가되고, 높은 농도의 이소루신(10∼30mM)에 의해서는 감소되는 s등 이소루신 생합성 과정에서 조절 역할을 하는 것으로 생각된다. The effects of branched chain amino acids and metabolites in growth media on the biosynthesis of Serratia marcescens threonine dehydratase activity were examined. The enzyme activity was decreased above 60% by leucine among the range from 1 to 20 mM, and the enzyme activity was decreased approximately 20% by a low concentration of valine(1 to 4 mM), but not affected at high concentration (20 mM). However, the enzyme activity was increased approximately 100 to 140% by a low concentration of isoleucine (1 to 4mM), but decreased approximately 25 to 80% at high concentration (15 to 30mM). The enzyme activity was decreased by 25 and 58% by the simultaneous addition of all three branched chain amino acids at 2 and 10mM concentration, respectively, but increased by 75 and 50% by the combination addition of isoleucine plus valine and isoleucine plus leucine at 2 mM, respectively. cAMP was decreased the enzyme activity approximately 10 to 40% by a low concentration (1 to 2 mM), but increased by 80% at high concentration (10 mM). These data suggest that S. marcescens threonine dehydratase should be multivalently repressed by branched chain amino acids, but positively regulated by a low isoleucine concentration and may play a regulatory role in an isoleucine biosynthetic pathway unlike the E. coli K-12 enzyme.

Serratia marcescens에서 글리옥실산이 Prodigiosin 생합성에 미치는 연구

최병범,방선권 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.4

최소 배지에 여러 아미노산과 대사 산물을 첨가하여 혐기성 조건하에서 배양시킨 Serratia marcescens ATCC 25419 세포 추출물에서 prodigiosin의 생합성을 조사한 결과 아미노산과 대사 산물들은 prodigiosin 생합성을 대조군보다 50∼80% 정도 감소시켰다. 글리옥실산은 1∼3mM에서 prodigiosin의 생합성을 대조군보다 20∼40% 정도 증가시켰고, 특히 5mM의 농도에서는 최대 122% 증가시켰으나, 20∼30mM에서 prodigiosin의 생합성을 50∼90% 정도 감소시켰다. 한편, 혐기성과 호기성 조건의 피루브산과 α-케토부티르산은 호기성 조건의 글리옥실산과 함께 조사한 모든 농도(0.5∼30mM)에서 prodigiosin이 생성되지 않았으며 또한, 혐기성 조건하에서 높은 농도(20mM 이상)의 글리옥실산은 S. marcescens의 성장을 현저하게 억제시켰다. 이러한 결과들로부터 글리옥실산은 피루브산과 α-케토부티르산과는 다르게 낮은 농도(1∼5mM)에서는 S. marcescens의 1, 2차 대사물질로 작용하여 prodigiosin 생합성을 증가시키지만, 높은 농도(20mM 이상)에서는 S. marcescens의 성장을 억제시켜 2차 대사물질인 prodigiosin의 생합성도 억제된다고 사료된다. The effects of amino acids and metabolites in growth media on the biosynthesis of prodigiosin from Serratia marcescens ATCC 25419 were examined. The prodigiosin synthesis was decreased approximately by 50 to 80% by several amino acids and metabolites tested. The prodigiosin synthesis was increased approximately by 20 to 40% by a low concentration of glyoxylate(1 to 3mM) and outstandingly increased by 122% at 5mM concentration under anaerobic condition. However, the prodigiosin synthesis was decreased approximately by 50 to 90% at a high concentration(20 to 30mM) under anaerobic condition. The prodigiosin was not synthesized by pyruvate and α-ketobutyrate under aerobic and anaerobic condition, with addition to glyoxylate under aerobic condition, among the range from 0.5 to 30mM, while the cell growth under anaerobic condition was decreased distinctly by high concentration(20mM above) of glyoxylate. These data suggest that the growth and prodigiosin of S. marcescens is positively regulated by a low concentration of glyoxylate (1∼5mM), but repressed by a high concentration of glyoxylate (20mM above) unlike pyruvate and α-ketobutyrate.

대중 매체 영향에 따른 대학생의 식품 안전 정보에 관한 연구

최병범,최성숙 한국식품영양학회 2010 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2010 No.06

Reversible Inactivation of Serratia Protease by 5,5'-dithiobis (2-nitrobenzoate)

최병범,김승수,Choi, Byung-Bum,Kim, Soung-Soo 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3

Serratia marcescens ATCC 25419로 부터 균체의 단백질 분해효소를 정제하고 이 효소의 활성에 대한 여러가지 sulfhydryl reagent 및 단백질 modifying reagent들의 영향에 관하여 조사하였다. Sulfhydryl reagent 중에서 p-chloromercuribenzoate, N-ethylmaleimide, dithiothreitol은 효소의 활성에 아무런 영향을 미치지 않았으나 5,5'-dithiobis (2-nitrobenzoate) (DTNB) 만이 효소의 활성을 가역적으로 억제하였다. DTNB에 의한 효소활성의 억제는 DTNB의 농도가 높아짐에 따라 증가하였고, 10 mM DTNB를 효소와 3시간 동안 pre-incubation하였을 때 효소활성이 완전히 억제되었다. 한편 특정 아미노산과 반응하는 시약 중 dansyl chloride와 diethylpyrocarbonate는 효소의 활성에 아무런 영향을 주지 못하였으나, pyridoxal-5-phosphate와 phenylglyoxal은 효소의 활성을 급격히 감소시키는 점으로 보아 효소의 active site나 또는 그 근처에 lysine과 arginine이 존재할 가능성이 있는 것으로 나타났다. 또한 DTNB와 pyridoxal-5 phosphate 또는 DTNB와 phenylglyoxal을 효소와 함께 pre-incubation시키면 DTNB만 효소와 pre-incubation시켰을 경우보다 효소의 활성이 누적적으로 억제되었다. 여러 dicarboxylated compound 중 aromatic benzene ring을 가진 phthalate가 이 효소의 활성을 현저히 억제하는 사실로 보아 DTNB의 영향도 aromatic ring과 dicarboxyl group이 중요할 것으로 예상되며, 특히 효소의 lysine 및 arginine residue와 DTNB의 noncovalent interactions의 가능성이 더 연구되어야 한다. The effect of sulfhydryl reagents and some amino acid modifying reagents on the purified extracellular protease from Serratia marcescens ATCC 25419 was examined. Among several sulfhydryl reagents tested, the activity of the enzyme was reversibly inhibited by 5,5'-dithiobis (2-nitrobenzoate)(DTNB), with the inhibition increasing as the concentration of DTNB was increased. Dithiothreitol, p-chloromercuribenzoic acid, iodoacetamide, and N-ethylmaleimide had virturally no effect upon the reaction velocities of the enzyme. The protease was also inhibited by pyridoxal-5-phosphate and phenylglyoxal, which suggests that lysine and arginine are present at the active site or near the active site of the enzyme. Effector studies with various dicarboxylated compounds revealed that phthalate having dicarboxylated benzene ring inhibited the protease considerably, while other dicarboxylated compounds without benzene ring had no significant inhibitory effects on the enzyme activity. A concentration and time-dependent reversible inactivation of Serratia protease by DTNB was increased cumulatively when DTNB plus pyndoxal phosphate or DTNB plus phenylglyoxal were preincubated with the enzyme for a given time before enzyme assay. It remains to be established whether DTNB inactivation of Serratia protease is the result of direct noncovalent interactions of DTNB with either lysine or arginine in the active site pocket or whether there exists some other distinct mechanism of inactivation.

아미노산과 대사산물들이 Serratia marcescens Biodegradative Threonine Dehydratase의 생합성에 미치는 영향

최병범,김승수 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1

최소 배지에 여러 아미노산을 첨가하여 배양한 Serratia marcescens ATCC 25419 세포 추출물에서 biodegradative threonine dehydratase의 비활성도를 조사한 결과 각각 10 mM의 isoleucine과 valine은 대조군보다 각각 44, 34% 감소시킨 반면 10 mM의 threonine은 20% 정도 증가시켰다. 여러 metabolite이 효소활성에 미치는 영향을 조사한 결과 pyruvate는 효소의 비활성도를 5 mM에서 60% 증가시켰으나, 20 mM 이상에서는 20 내지 70% 정도 감소시켰다. Glyoxylate는 효소의 비활성도를 5 mM에서 64% 증가시켰으나, 20 mM 이상에서 40 내지 70% 정도 감소시켰다. Monopyrrole 유도체인 thiamine은 효소의 비활성도를 50 ㎍/㎖에서 84% 증가시켰고, 300 ㎍/㎖ 이상에서는 비활성도에 영향을 주지 못하였다. cAMP는 효소의 비활성도를 0.5 mM에서 58% 증가시킨 반면, 2 mM에서는 비활성도를 15% 감소시켰다. The effects of amino acids in growth media on the biosynthesis of Serratia marcescens biodegradative threonine dehydratase activity were examined. The enzyme activity was decreased by 44 and 34% by 10 mM isoleucine and valine, respectively, whereas it was increased approximately by 20% by 10 mM threonine. Among several metabolites tested, pyruvate increased the enzyme activity by 60% at 5 mM, but decreased the enzyme activity approximately by 20 to 70% above 20 mM. The enzyme activity was increased by 64% by 5 mM glyoxylate, whereas it decreased the enzyme activity approximately by 40 to 70% above 20 mM glyoxylate. The thiamine, monopyrrole derivative, also increased the enzyme activity by 84% at 50 ㎍/㎖, but did not affected the enzyme activity above 300 ㎍/㎖. cAMP increased the enzyme activity by 58% at 0.5 mM, but decreased the enzyme activity by 15% at 2 mM. These data suggested that the biosynthesis of Serratia marcescens biodegradative threonine dehydratase is regulated by concentrations of pyruvate, glyoxylate and cAMP.