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Tae Il Moon(문태일),Kwan Soo Choi(최관수),Hyun-Hwoi Ku(구현회) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11
The amount of complex fertilizer (N-P₂O<SUB>5</SUB>-K₂O) used by Korean farms was 921 tons, which is the most applied in agricultural soils as of 2017 years. The complex fertilizer generates ammonia (NH₃) gas, which is a precursor to secondary particulate matter (PM<SUB>2.5</SUB>). It is vital to understand the emission characteristics as ammonia emission is dependent on the source of nitrogen fertilizer input and level of application. This study was conducted to identify the emission characteristics of ammonia by fertilizer input during the cultivation of pears. Four levels (0, 25, 50 and 100kg N ha<SUP>-1</SUP>) of complex fertilizer were applied to the soil (loamy soil). To determine cumulative ammonia emission during the season (early March to end of Octorber in 2021 year) we trapped and measured the gas concentration from the soil using dynamic chamber method. The sampling was done at least twice per week and immediately analyzed NH₃ concentration using nesslerization method. Result showed that the the application of fertilizer produced NH₃ emission and showed the peak within 5 days after application followed by a decreasing trend. Cumulative NH₃ emissions in each treatment were 2.0 (N 0), 2.3 (N 25), 2.6 (N 50), and 2.9 (N 100) kg NH₃ ha<SUP>-1</SUP> season<SUP>-1</SUP>. Applying the each cumulative amounts of NH₃ emission in the treatment, an ammonia emission factor from complex fertilizer was 8.9 kg per tons (Y=0.0089x+2.066, R²=0.953*) (Fig. 1). Our result could enable us to investigate ammonia emission factor from complex fertilizer during the pear cultivation period evaluate emission characteristics. 〈수식 본문참조〉
U-937 세포에서 세라마이드의 세포증식과 세포주기 조절단백질에 대한 작용
이재훈(Jae Hoon Lee),최관수(Kwan Soo Choi),김미영(Mie Young Kim) 대한약학회 1997 약학회지 Vol.41 No.1
Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger ymediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C2-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D1 and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.
IM-9 세포에 있어서 세라마이드에 의한 세포주기 변화와 아포프토시스
윤기호(Ki Ho Yoon),최관수(Kwan Soo Choi),김원호(Won Ho Kim),최경희(Kyung Hee Choi),김미영(Mie Young Kim) 대한약학회 1995 약학회지 Vol.39 No.6
Sphingolipids play important roles in cell regulation and signal transduction. Recently, a sphingomyelin cycle has been described in which activation of neutral sphingomyelinase leads to the breakdowm of sphingomyelin and the generation of ceramide. Ceramide, in turn, has emerged as a candidate intracellular mediator for the action of certain cell agonists and has multiple biologic actions. Ceramide is a potent suppressor of cell growth and an inducer of apoptosis. The present studies show that exposure of IM-9 cells to ceramide resulted in internucleosomal cleavage of DNA, yielding laddered patterns of oligonucleosomal fragments characteristic of apoptosis. DNA fragmentation induced by ceramide was also confirmed by diphenylamine assay. The effect of ceramide on cell cycle progression was also studied. The addition of ceramide increased G1 phase distribution in cell cycle, Cell cycle-related cyclin D1 gene expression was decreased in a time-dependent manner. These results suggest that apoptosis induced by ceramide is related to cell cycle associated with the alteration of cell cycle in IM-9 cells.
비린내와 조직감이 개선된 치즈 토핑 반건조 조미 피조개(Scapharca broughtonii)의 개발 및 특성
강상인 ( Sang In Kang ),김예진 ( Ye Jin Kim ),이지운 ( Ji Un Lee ),박지훈 ( Ji Hoon Park ),최관수 ( Kwan Su Choi ),황지영 ( Ji-young Hwang ),허민수 ( Min Soo Heu ),이정석 ( Jung Suck Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6
Methods for the development of home meal replacement seafood tailored to consumer needs for the advanced use of Broughton’s ribbed ark Scapharca broughtonii (BRA) in Korea are required. In this study, we developed a cheesetopped, semi-dried, and seasoned Broughton’s ribbed ark (S-BRA) tailored for the younger generation with an improved texture and fish odor. The optimization of conditions to improve the texture and fish odor was performed using RSM. The design of the model was appropriate because there was no significant difference (P>0.05) between the predicted and actual values of moisture content, hardness, and overall acceptance, and the optimal preparation conditions were a vinegar content of 2.68%, a soaking time of 62 min, a drying temperature of 60℃, and a time of 162 min. The SBRA manufactured under these optimal conditions exhibited a lower odor intensity compared to the unsoaked and undried control, suggesting that the fish odor of S-BRA has been improved. The moisture content related to the texture of the S-BRA was lower than that of the control, and the hardness was higher. Therefore, the S-BRA developed in this study will appeal to people of all ages, especially the younger generation; their consumption is expected to increase.
갓(Brassica juncea)을 첨가한 간장 피조개(Scapharca broughtonii)장의 개발 및 관능특성
강상인 ( Sang In Kang ),김예진 ( Ye Jin Kim ),이지운 ( Ji Un Lee ),박시형 ( Si Hyeong Park ),최관수 ( Kwan Su Choi ),송호수 ( Ho-su Song ),최정미 ( Jung-mi Choi ),허민수 ( Min Soo Heu ),이정석 ( Jung Suck Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6
The home meal replacement (HMR) market has attracted significant attention due to COVID-19 pandemic and products that utilize the combination of different processed ingredients are now being developed. In this study, Broughton’s ribbed ark Scapharca broughtonii was soaked in seasoned soy sauce with the incorporation of mustard leaf Brassica juncea (M-BRA), which is known to have a unique texture as well as excellent functional properties such as antioxidation, to develop a regional specialty product. The optimal conditions for manufacturing M-BRA from the seasoned soy sauce (the sauce to be added [X<sub>1</sub>] and the soaking time [X<sub>2</sub>]), were examined using response surface methodology (RSM) to analyze the significance of the salinity (Y<sub>1</sub>), amino-N content (Y<sub>2</sub>), and overall acceptance (Y<sub>3</sub>). The coefficient of determination (R<sup>2</sup>) between X<sub>1</sub>-X<sub>2</sub> and Y<sub>1</sub>-Y<sub>3</sub> were close to 1, thereby confirming the suitability of the RSM model. The optimal conditions were seasoned soy sauce addition of 140% and soaking time of 156 min. The M-BRA manufactured under these conditions exhibited superior overall acceptance compared to seasoned commercial soy sauce, which was used as a control. We expect that the market for M-BRA manufactured by combining marine and agricultural materials will expand owing to superior overall acceptance compared with commercial products.
윤기호,최관수,이강균,김미영 중앙대학교 약학연구소 1995 약학 논총 Vol.9 No.-
Sphingolipids play important roles in cell regulation and signal transduction. Recently, a sphingomyelin cycle has been described in which activation of neutral sphingomyelinase leads to the breakdown of sphingomyelin and the generation of ceramide. Ceramide, in turn, has emerged as a candidate intracellular mediator for action of certain cell agonists and has multiple biologic actions. Ceramide is potent suppressor of cell growth and an inducer of apoptosis. The present studies show that exposure of IM-9 cells to ceramide resulted in internucleosomal cleavage of DNA. DNA fragmentation induced by ceramide was confirmed by ^3H-thymidine release and diphenylamine assay. Transmission electron micrographs of IM-9 cells treated with ceramide showed typical apoptotic morphology with marked nuclear chromatin condensation and apoptotic bodies. These results show a role for ceramide in the regulation of cell growth and apoptosis.