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인간 간암세포주에서 nitrosative stress에 대한 글루타치온 환원효소의 방어적 역할
지상미,임창진 강원대학교 기초과학연구소 2010 기초과학연구 Vol.21 No.-
Glutathione reductase (GR)은 cell 내에서 cofactor인 NADPH의 존재 하에 산화된 형태의 glutathione (GSSG)를 환원된 형태인 glutathione (GSH)로 변환시켜, cell을 안정시켜준다고 알려져 있다. Glutathione은 중요한 cell 내 항산화분자이다. 이미 이전에 실행된 실험에서 GR이 과발현된 HepG2 transfectant를 이용해 GR이 oxidative 스트레스에 대해 방어적인 역할을 한다는 것이 입증되었다. 현재 진행된 연구는 nitrosative 스트레스에서 GR의 세포내 역할을 해명하기 위해 수행되었다. GR이 과발현된 transfectant가 sodium nitropursside (SNP, 0.25, 0.5, 1 mM)에 의해 생성된 nitricoxide (NO)가 존재하는 환경에서 mock-transfectant보다 생존률이 증가하고, reactive oxygen species (ROS) level이 변함 없음을 알 수 있었다. 또, mock-transfectant 전체 GSH농도는 급격히 감소했지만, GR 과발현 transfectant는 증가하는 것을 확인할 수 있었다. 하지만 GR 과발현 transfectant와 mock-transfectant에서 NO level은 비슷하게 관찰되었다.
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인삼 총 사포닌의 에스트로젠, 안드로젠, 레티노익산 호르몬 수용체에 대한 활성
지상미(SangMi Ji),이영주(YoungJoo Lee) 고려인삼학회 2003 Journal of Ginseng Research Vol.27 No.3
Alternative or complementary medicine plays an important role in health care system. Ginseng, being one of the most popular oriental herbs, is believed to contain various steroid hormone activity. Ginseng has been demonstrated pharmacological effect in the cardiovascular, endocrine, central nervous, and immune system. Our objective was to study that total saponin might mediate some of their actions by binding to the steroid hormone receptor, as they share many of the actions of steroid hormone in various physiological system. Using total saponin from Panax Ginseng, we have studied the possibility of total saponin being a potential estrogen receptor, androgen receptor, and retinoic acid receptor ligand. Total saponin activated the transcription of both the estrogen and androgen responsive luciferase reporter plasmids at a<br/> concentration of 100 μg/ml in COS cells transiently transfected with the corresponding receptor and hormone responsive receptor plasmids. And total saponin caused a concentration-dependent stimulation of estrogen receptor. Total saponin increased the expression of estrogen responsive c-fos proto-oncogene at the protein level in MCF7 cells at 24 h treatment as examined by Western analysis. The c-fos induction was used as a specific marker of estrogen responsiveness. This activation was inhibited by the specific estrogen receptor antagonist, ICI 182,780. However, total saponin failed to activate the retinoic acid receptor in COS cells transiently transfected with the corresponding receptor and retinoic acid responsive reporter plasmids. These results show that total saponin is capable of activating estrogen and androgen receptors.
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배윤성,조정윤,지상미,이영주 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.3
Gene therapy is fundamentally a sophisticated drug delivery technology to cure a disease by the transfer of genetic material to modify living cells, In other words, the gene is used as a therapeutic drug much like a chemical compound is employed in chemotherapy. Currently almost 600 clinical trials are underway worldwide since the first clinical trials carried out in 1990 to treat adenosine deaminase deficiency using retroviral vectors. Despite the great progress still is there no gene therapy product being approved as a new drug. This is partly due to a lack of and ideal gene delivery system that is safe and can provide stable, optimal level production of the therapeutic proteins in the cell. This review covers the current status of several different biological and physico-chemical agents that are being developed as gene delivery vehicles. Although gene therapy promises great hopes toward the cure of a broad spectrum of genetic and acquired diseases, the success of gene therapy heavily asks for the development of vector systems for safe and efficient application in humans.
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국내에서 개발된 GM 쌀 (밀양 204호, 익산 483호)에대한 정성 PCR 분석법 개발
김재환,김해영,송희성,지상미,류태훈,김동헌 한국응용생명화학회 2005 Journal of Applied Biological Chemistry (J. Appl. Vol.48 No.4
국내에서 개발된 GM 쌀인 밀양 204호, 익산 483호의 정성 PCR 분석법의 개발을 위해 쌀의 내재 유전자로써 SAMDC1(S-adenosylmethionine decarboxylase)에 특이적인 primer (OsSAMDC1-5'/3')쌍을 제작하여 쌀을 포함한 20개 작물에 대해 PCR을 수행하여 쌀에 특이적으로 증폭되는 것을 확인하였다. 또한, 밀양 204호에 삽입된 GUS 유전자와 NOS terminator 연결 부위를 증폭시켜 172 bp의 PCR 산물을 얻을 수 있는 primer(Os204-5'/OsNOS-3')와 익산 483호에 삽입된 bar 유전자 와 NOS terminator 연결 부위를 증폭시켜 161 bp의 PCR 산물을 얻을 수 있는 primer(Os483-5'/OsNOS-3')을 이용하여 국내 개발된 GM 쌀인 밀양 204호, 익산 483호의 PCR 정성 분석법을 확립하였다. For the development of qualitative PCR detection method of genetically modified rice (Oryza sativa L.), rice species specific gene, SAMDC1 (S-adenosylmethionine decarboxylase), was selected and validated as suitable for use as an endogenous reference gene in rice. The primer pair OsSAMDC1-5'/3' with 110 bp amplicon was used for amplification of the rice endogenous gene,SAMDC1 and no amplified product was observed from 19 different plants as templates. Qualitative PCR method was assayed with 2 different GM rices (Milyang 204 and Iksan 483) developed in Korea. For the qualitative PCRs, the construct-specific detection primer pairs were constructed.Os204-5'/OsNOS-3' amplifying the junction region of GUS gene and NOS terminator introduced in Milyang 204 gave rise to an amplicon 172 bp; also, Os483-5'/OsNOS-3' amplifying the junction region of Bar gene and NOS terminator introduced in Iksan 483 gave rise to an amplicon 161 bp.
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