영지버섯 추출물을 이용한 제빵의 특성

정현채(Hyun-Chae Chung),이준탁(Jun-Tak Lee),권오진(Oh-Jin Kwon) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.7

각종 성인병 및 항암작용에 탁월한 효과가 입증된 영지버섯을 제빵시 첨가하여 기능성이 부여된 빵을 개발하고자 본 연구를 수행하였다. 영지추출액을 8%까지 첨가함에 따라 쓴맛이 증가하였지만, 1차 발효기간 동안 반죽의 부피는 증가하였고 굽기 손실률이 감소함을 관찰할 수 있었다. 영지추출물을 첨가한 빵의 관능검사결과, 2% 첨가시 유의적인 차이가 나타나지 않았으나, 4, 6, 8%를 첨가했을 때 4%는 보통으로 6, 8%는 대조구에 비해 나쁘게 나타났다. 사진촬영 결과 역시 0, 2% 영지추출액 첨가식빵의 내부조직은 우수하였으나, 4%는 보통으로 6, 8%는 나쁘게 평가되었다. 이러한 사실들로 보아, 일반식빵과 차이가 없는 2% 영지추출액 첨가시 기능성이 부여된 식빵의 제조가 가능하리라 생각한다. The purpose of this study was to develop functional breads added with Ganoderma lucidum (GL) extract which was well known for preventing various adult diseases and cancer. It was observed that bitter taste was increased with up to 8% GL extract while dough volume was increased at the first dough fermentation period and baking loss reduction was decreased. In terms of sensory evaluation, there were not so much significant differences with 2% added GL extract. In 4, 6, 8% added GL extract bread, it was found that 4% was ordinary and 6% and 8% were bad compared to the control bread. The bread with 2% added GL extract was excellent, 4% was ordinary and 6, 8% was badly evaluated in the internal texture. It’s possible to make the functional breads baking with 2% GL extract which is similar to the control breads based on the above results.

Cerulein 유발 급성 췌장염에 대한 nafamostat mesilate의 예방 효과

이준규 ( Jun Kyu Lee ),박주경 ( Joo Kyoung Park ),이상협 ( Sang Hyub Lee ),윤원재 ( Won Jae Yoon ),류지곤 ( Ji Kon Ryu ),김용태 ( Yong Tae Kim ),정현채 ( Hyun Chae Jung ),윤용범 ( Yong Bum Yoon ) 대한내과학회 2007 대한내과학회지 Vol.72 No.4

목적: 급성 췌장염의 동물 모델에 있어서 여러 가지 단백 분해 효소 억제제들의 예방 효과가 입증되어 있으나 질병 발생 이후에야 약물이 투여되는 실제 임상 상황에서는 단백 분해 효소 억제제들의 급성 췌장염 대한 치료 효과는 확실하지 않다. 본 연구자는 대부분의 동물실험에서 췌장염 유발 전에 투여되었으며, 유발 전과 후에 각각 투여하여 그 결과를 비교한 실험은 찾아보기 어렵다는 사실에 착안하여, 약물의 투여 시점이 급성췌장염의 예방 혹은 치료 효과에 중요하다는 가설을 세우고 단백 분해 효소 억제제인 nafamostat mesilate (NM)을 췌장염 유도 전후에 각각 생쥐에 투여하여 그 효과를 비교하였다. 방법: 생쥐에서 콜레시스토키닌(cholecystkinin) 유사 물질인 cerulein을 반복적으로 복강내 투여하여 급성췌장염을 유발시키면서 투여 전후에 각각 NM를 정맥내 주사하고 혈청 아밀라제 및 리파아제, 췌장 조직내 MPO (myeloperoxidase) 활성도, 조직학적 변화 등을 측정하여 각군에 있어서 염증의 정도를 비교하였다. 또한 간 군에 있어서 p38 MAPK (mitogen-activated protein kinase)와 그의 활성형인 phosphor-p38 MAPK, 그리고 IL-6(interleukin-6)의 췌장 조직 내에서의 발현 양상을 살펴보았다. 결과: Cerulein의 반복적인 복강내 투여를 통해 급성췌장염이 성공적으로 유발되었다. NM을 췌장염 유발 전에 투여할 경우 예방 효과가 있었던 반면, 유발 후에 투여하였을 경우에는 그 효과가 확실하지 않았다. 급성췌장염이 발생한 군에서는 p38 MAPK에 비하여 phosphor-p38 MAPK의 발현이 증가되어 있었던 반면, 각 군 간에 있어서 IL-6의 발현에는 차이가 없었다. 결론: NM을 급성췌장염 발생 이후 치료목적으로 사용할 경우 그 효과는 확실하지 않으나, 내시경적역행성 담췌관조영술과 같이 급성췌장염의 발생이 예상되는 경우에 예방을 위해 미리 투여하는 것은 효과가 있을 것으로 기대된다. Background: Many protease inhibitors show a protective effect for acute pancreatitis as seen in animal models. In previous studies, the protease inhibitors were administered before induction of pancreatitis, and there are few published reports examining effects when these agents were administered after induction of pancreatitis. The timing of drug administration may provide an explanation for the ineffectiveness of protease inhibitors for the treatment of patients with acute pancreatitis. Herein, we assessed the protective effect of nafamostat mesilate (NM), a potent protease inhibitor, in a mouse model of cerulean-induced pancreatitis and compared the results of administering the drug before and after the induction of pancreatitis. Methods: Cerulein, a cholecystokinin analogue, was injected into mice intraperitoneally to induce pancreatitis. The mice received intravenous NM administration before and after the induction of pancreatitis. The serum concentration of amylase and lipase was measured, histological changes were measured, and the tissue expression of myeloperoxidase was measured to assess the degree of inflammation. Expression of p38 MAPK (mitogen-activated protein kinase), phospho-p38 MAPK, and IL-6 (interleukin-6) in tissue was evaluated. Results: Acute pancreatitis was induced successfully by intraperitoneal injection of cerulein. Acute pancreatitis could be prevented when NM was administered before the induction of pancreatits. However, the effect was not guaranteed when given after the induction of pancreatitis. For a group of mice with induced pancreatitis, tissue expression of phospho-p38 MAPK was prominent and there was no marked difference in the expression of IL-6 between groups with or without induced pancreatitits. Conclusions: Although the efficacy of NM for treatment of acute pancreatitis is doubtful, pretreatment with NM for an expected condition like endoscopic retrograde cholangiopancreatography (ERCP), might be helpful for the prevention of pancreatitis. (Korean J Med 72:340-351, 2007)

장기간 스테로이드 및 Sulfasalazine 투여를 받아 온 아메바성 대장염 의 임상경과

이준규(Jun Kyu Lee),석웅(Woong Seok),천재희(Jae Hee Chun),김유선(You Sun Kim),최일주(Il Ju Choi),김주성(Joo Sung Kim),정현채(Hyun Chae Jung),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 2000 대한소화기학회지 Vol.36 No.3

Many of water-borne infectious diseases are under control nowadays because of the improvement of hygiene and sewage system, but some of them (e.g. amebic colitis) are still endemic in developing countries. Since there is no definite method of diagnosis, amebic colitis is often diagnosed too late or misdiagnosed as idiopathic inflammatory bowel disease. There have been some reports that treatment with glucocorticoids or immuno-suppressive agents may lead to catastrophic result in amebic colitis. We experienced a 65-year-old man who showed somewhat different clinical consequences. He had longstanding hematochezia with lower abdominal pain, and was misdiagnosed as ulcerative colitis. Because his symptom continued over a year despite treatment with glucocorticoids and sulfasalazine, total colectomy was considered. However, the diagnosis of amebic colitis was made with repeated stool parasite examinations, colonoscopic biopsy and serologic tests. He showed dramatic improvement after the antiamebic therapy with metronidazole. (Kor J Gastroenterol 2000;35:398 - 403)

1996년도 제35차 대한소화기학회 추계학술대회 / 포스터 발표 : 위장관 ; 염증성 장질환 환자에서 소장통과 시간

김유선,정준오,정현채,송인성,김정룡 ( You Sun Kim,Jun Oh Jung,Hyun Chae Jung,In Sung Song,Chung Yong Kim ) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.1

크론병에서 Infliximab 치료의 효과와 안전성 - 단일 기관에서의 경험

최기돈 ( Kee Don Choi ),송호준 ( Ho Jun Song ),김주성 ( Joo Sung Kim ),정현채 ( Hyun Chae Jung ),송인성 ( In Sung Song ) 대한소화기학회 2005 대한소화기학회지 Vol.46 No.1

국내 노인 위암 환자의 임상적 및 병리학적 특성

김연주 ( Youn Joo Kim ),이창현 ( Chang Hyun Lee ),백우현 ( Woo Hyun Paik ),김나영 ( Na Young Kim ),박영수 ( Young Soo Park ),정숙향 ( Suk Hyang Jung ),황진혁 ( Jin Hyeok Hwang ),이광혁 ( Kwang Hyuck Lee ),정현채 ( Hyun Chae Jun 대한내과학회 2007 대한내과학회지 Vol.72 No.3

목적: 위암은 국내에서 발생률이나 사망률로 볼 때 가장 중요한 암 가운데 하나이다. 빠르게 증가하는 국내 노인 인구를 감안할 때 노년층에서의 위암 발생도 계속 증가할 것으로 보인다. 노년층 위암의 임상, 내시경, 조직 특성, 수술과 관련한 회복기간과 합병증률을 분석함으로써 노인 위암의 진단, 치료 방법 결정의 기초자료를 얻고자 하였다. 방법: 2003년 5월부터 2005년 5월까지 분당서울대학교 병원에서 위암으로 진단된 환자 중 수술을 받은 268명의 환자를 대상으로 후향적으로 조사하였다. 환자들을 연령에 따라 65세 미만의 장년층과 65세 이상의 노년층으로 구분하여 두 군에 대해 성별, 가족력, 증상, 암의 위치 및 형태, 병기, 병리를 비교하였으며 수술과 관련하여 ASA 위험도, 수술 후 합병증, 회복기간 등을 비교하였다. 결과: 두 군 간 성별, 증상 유무, 가족력 유무는 통계적으로 유의한 차이를 보이지 않았다. 수술 전 노년층에서 ASA 고위험 군의 비율이 높은 것으로 나타났으나, 수술 후 회복기간은 장년층에서 평균 10.11일, 노년층에서 11.58일로 유의한 차이가 없었다(p=0.374). 수술 후합병증 발생률은 양 군간 통계적으로 유의한 차이가 없었다. 양 군에서 조기위암과 진행성 위암의 차이, 병기에서 차이는 없었다. WHO 분류에서 장년층은 저분화암(41.2%), signet ring cell (20.1%), 노년층에서 고분화암(27.4%)이 각각 통계적으로 유의하게 높은 빈도를 보였다(p<0.001). Lauren 분류에서 장년층은 미만성(58%), 노년층은 장형(59.4%)이 가장 높은 빈도를 보였다(p<0.001). MSI-H의 빈도는 장년층 5.6%, 노년층 17%으로 노년층에서 통계적으로 유의하게 높은 발현율을 보였다(p=0.010). p53의 발현율은 양 군 간 차이가 없었다. 결론: 노인 위암 환자는 장년 환자에 비해 예후가 좋다고 알려진 병리 형을 갖는 경우가 많았으며 림프절 전이를 줄임으로써 좋은 예후인자가 된다고 알려진 MSI-H의 빈도가 높았다. 또한 노인 위암 환자에서 장년 위암 환자에 비해 수술 후 회복기간이나 합병증 발생률의 차이를 보이지 않았다. 그러므로 노인 위암 환자에 대한 수술적 치료를 좀 더 적극적으로 고려할 필요가 있다. Background: Gastric cancer in elderly patients is one of the most important health issues in Korea, when considering its incidence and mortality rate. We have obtained the preliminary data concerning the diagnosis and treatment of gastric cancer in elderly people by analyzing the clinical and pathologic features. Methods: We retrospectively analyzed 268 patients who were diagnosed with gastric cancer and who also underwent surgical procedures in Bundang Seoul National University Hospital from May 2003 to May 2005. The patients were divided into two groups: those aged<65 (n=162) and those aged≥65 (n=106). We compared the clinical and pathological characteristics between the older and younger groups. Results: The elderly group had more patients with well differentiated carcinoma (11.8% vs. 33.0%, respectively, p=0.001) and the intestinal type (34.0% vs. 59.4%, respectively, p<0.001). The frequency of microsatellite instability-high (MSI-H) was higher in the elderly group (5.6% vs. 17%, respectively, p=0.010). There was no significant difference in the postoperative recovery time (p=0.374) and the operation-related complication rate between the two groups. Conclusions: Gastric cancer in elderly patients demonstrated more pathologic types that have a known favorable outcome when compared to the younger patient group. We should more frequently consider surgery for the elderly patient with operable gastric cancer because of they have similar postoperative recovery compared to the younger patients. (Korean J Med 72:256-265, 2007)

고프롤린식이에 의한 백서 소장에서의 ACE과 DAP-IV 유도의 유전적 조절기전에 대한 연구

이동호(Dong Ho Lee),김재준(Jae Jun Kim),임선희(Sun Hee Lim),윤병철(Byung Chul Yoon),이국래(Kook Lae Lee),정현채(Hyun Chae Jung),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.3

N/A Background/Aims: Angiotensin converting enzyme(ACE) and Dipeptidyl aminopeptidase IV(DAP- IV) are well known brush border rnembrane enzymes which have major role in digestion of prolyl peptide(proline). Even though high proline diet is known to induce the increase of enzymatic activity of ACE and DAP-IV, the genetic mechanism of this phenomenon has not been recognized. Therefore we performed this study to clarify the genetic mechanism of ACE and DAP-IV induction by high proline diet. Methods: Total 20 Wistar rats were divided into four groups with different diets. Control group was fed with normal diet(17% protein), sucrose group was fed with high sucrose diet(68% sucrose, 18% protein), protein group was fed with high protein diet(50% casein), and gelatin group was fed with high proline diet(50% gelatin). After 3 weeks the small intestine was removed and divided into three equal length segments, from which homogenate, btesh border membrane and total RNA were prepared. ACE and DAP-IV enzyme activity were estimated in both small intestine homogenate and brush border membrane. The amounts of ACE mRNA and DAP-IV mRNA were also estimated by Northern analysis of RNA. Results: ACE activity of homgenate: There was significant increase of ACE activity in gelatin group(pC0.01) and protein group(p(0.05) throughout all sites(proximal, middle, distal). ACE activity of brush border membrane: There was significant increase of ACE activity in distal site(p0.01) of gelatin group and in middle(p0.05) and distal site(p0.05) of protein group. DAP-IV activity of homogenate: There was significant increase of DAP-IV activity in middle(p<0.01) and distal(p(0.05) site of gelatin group. DAP-IV activity of brush border membrane: There was significant increase of DAP-IV activity in gelatin group(p(0.01) and protein group(pC0.01) throughout all sites. ACE mRNA amount: Gelatin group showed increase of ACE mRNA in proximal site(p C0.05) and total mean value(p0.05). Protein group also showed increase of ACE rnRNA in proximal(pC0.05)), middle(pa0.05) and total mean value(p0.05). DAP-IV mRNA amount: There was no statistically significant change in gelatin and protein group. Conclusions: 1) High proline diet induced the increase of enzymatic activity of ACE and DAP-IV. 2) Increase of ACE activity was associated with increase of ACE mRNA in small intestine. 3) Increase of DAP-IV activity was not correlated with DAP-IV mRNA. These results suggested that the induction of increased ACE activity by high proline diet is regulated on transcriptional level and the induction of increased DAP-IV activity may be regualted on posttranscriptional level (translational and/or posttranslational level). In conclusion, the induction of increased enzyme activity of brush border membrane by specific diet may be regulated on both transcriptional level and posttranscriptional level. (Korean J Gastroenterol 1996; 28:349 - 361)

원인불명의 단백상실성 장병증

송인성(In Sung Song),김정룡(Chung Yong Kim),김용일(Yong Il Kim),정현채(Hyun Chae Jung),정준기(June Key Chung),김윤준(Yoon Jun Kim),임영석(Young Seok Lim),이대희(Dae Hee Lee),우광훈(Gwang Hoon Woo),이국래(Kook Lae Lee),한준구(Joon Koo 대한소화기학회 1997 대한소화기학회지 Vol.29 No.3

Protein-losing enteropathy is a mamfestation of various disorders associated with an excess loss of serum proteins into the gastrointestinal tract, thereby leading to hypoproteinemia and other sequelae. We present here a case of protein-losing enteropathy with unknown etiology. In spite of explorative laparotomy for multiple stricture of the small bowel, we were unable to uncover the etiology of protein-losmg entetopathy. After the resection of most severe stricture site, serum protein level was normalized, (Korean J Gsstroenterol 1997; 29: 416-421)

우리나라에서의 Crohn 병 - 임상적 , 병리학적 고찰 및 치료에 따른 경과 관찰 -

이종균(Jong Kyun Lee),김유철(You Cheoul Kim),김재준(Jae Jun Kim),이풍렬(Poong Lyul Rhee),최상운(Sang Woon Choi),정현채(Hyun Chae Jung),윤용범(Yong Bum Yoon),송인성(In Sung Song),최규완(Kyoo Wan Choi),김정룡(Chung Yong Kim),이승숙(Seu 대한내과학회 1991 대한내과학회지 Vol.41 No.5

N/A Crohn's disease used to be recognized as a rare inflammatory bowel disease in Korea, but current accumula- tion of sporadic case reports suggests a gradual increase of its inridence. Systemic studies, however, with clinical follow-up after treatment have not been fully assessed in the era of advanced colonoscopic approach. This article describes the clinical and pathologic findings of 15 cases of Cron's disease together with their clinical follow-ups with medical therapy, experienced during a period from October, 1978 to July, 1990. The results are summarized as follows: 1) The male-to-female ratio was 3,1, and the mean age was 25.5 years. The duration of symptoms was 2 months to 4 years, and the mean duration was 26 months. Common symptoms and signs included diarrhea, abdominal pain, weight loss, fever, etc., and the extraintestinal manfestations were rather scarce in these study cases. Anemia, increased ESR, throm-bocytosis, and hypoalbuminemia were frequent findings. 2) Colonoscopic and/or radiologic studies showed skipped lesion (80%), cobblestone appearance (67%), and longitudinal ulcer (47%) in order of frequency. The most common involved sites were the small bowel alone (27%), ileocolitis (60%), and the colon alone (13%). 3) Most of the patients (67%) responded well to medical therapy for a short-term period, and there was a good correlation between the subjective symptoms, colonscopic or radiologic findings, and Crohn's disease activity index. 4) Compared with cases from Western countries, the sex ratio in this study showed a male predominance, extraintestinal manifestation was rare, many patients (53%) had a past medical history of failure with anti-tuberculosis medication, and a good correlation existed between the subjective symptoms an6 objective examination. With the above findings, we assume that Crohn's disease is still a rare inflammatory bowel disease in Korea which requires a differential diagnosis with intestinal tuberculosis. It also seems more likely that in patients with clinical features of intestinal tuberculosis but who did not show any evidence of clinical improvement, including objective findings of intestinal ulcer, in spite of prolonged anti-tuberculosis medication lasting for several months, a possibility of Crohn's disease should be considered.

재래식 간장의 한외여과시 공급액의 주입속도와 잔류액의 용량감소율이 간장의 투과유속, 미생물균체 및 성분저지율에 미치는 영향

권광일(Kwang-Il Kwon),이종구(Jong-Gu Lee),최종동(Jong-Dong Choi),정현채(Hyun-Chae Chung),임무혁(Moo-Hyeong Im),김기주(Ki-Ju Kim),김우성(Woo-Seong Kim),성준현(Jun-Hyun Sung),권오준(O-Jun Kwon),김영지(Young-Ji Kim),서정식(Chung-Sik Suh 한국식품과학회 2003 한국식품과학회지 Vol.35 No.1