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생쥐난자의 성숙단계와 결빙억제제에 따른 동결 및 해빙 후 생존율에 관한 연구
최규완,이호준,강희규,전용필,김문규,Choi, Kyoo-Wan,Lee, Ho-Joon,Kang, Hee-Kyoo,Chun, Yong-Pil,Kim, Moon-Kyoo 대한생식의학회 1991 Clinical and Experimental Reproductive Medicine Vol.18 No.1
In order to increase the pregnancy rate by means of cryopreservation of the excess oocytes in IVF-ET program, the survival rate of the frozen-thawed oocytes of mouse was examined according to the stages of maturation, cryoprotectants and their treatment. The results were summarized as follows. First, during the continuous treatment with cryoprotectant media, the survival rate of oocytes was higher in DMSO than in PROH, and higher at low temperature($4^{\circ}C$) than at room temperature($25^{\circ}C$). Second, as regard with the maturation of immature(GV-intact) oocytes after treatment with cryoprotectant media, the rate of maturation in DMSO-treated group(52%) was higher than in PROH-treated group(35%). Third, according to the treatment of cryoprotectant media, the survival rate of frozen-thawed oocytes in DMSO-treated group (45%) was higher than in PROH-treated group(29%), and that of oocytes in DMSO 4-step treated group was higher than any other groups. Finally, in the post-thaw oocytes frozen at various stage of maturation, the survival rate of immature oocytes with GV was the highest in all groups. These results suggest that in the cryopreservation of mouse oocytes, DMSO was better than PROH as cryoprotectant, in treatment of cryprotectant the multi-step treatment was better than single-step, and the post-thaw survival rate of oocytes was closely related to the maturity of oocytes. It is assumed that the highest survival rate of mouse oocytes with GV is due to the stability of the structures in nucleus and intracelluar organelles, and of physiological function.
투명대하 미세수정(SUZI)시 정자의 상태 및 처리방법에 따른 수정률과 임신률
최규완,김수경,양현원,차영범,이승재,박종민,김문규,Choi, Kyoo-Wan,Kim, Soo-Kyung,Yang, Hyun-Won,Cha, Young-Beom,Lee, Seung-Jae,Park, Jong-Min,Kim, Moon-Kyoo 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
Subzonal insemination(SUZI) has been proposed for patients with severe male factor and previous fertilization failure. However, very low fertilization rates still persisted. The aims of this study were firstly, to examine the relationships between the fertilization rate and sperm parmeters, sperm incubation media and time, secondly, to evaluate the outcome of 119 cycles of SUZI applied the modified sperm preparation method. The fertilization rates were influenced more sensitively by sperm preincubation media and time than by sperm parameters. According to preincubation media and time, the fertilization rates were 43.3% in 50% follicular fluid (HFF), 36.6% in 10% fetal cord serum(FCS), and with the time, increased in FCS, but decreased in HFF. In regrd with sperm parameters, the fertilization rates were 42.9% in normal and 37.6% in subnormal group. The best results were obtained from SUZI by the spermatozoa incubated in 50% HFF for 6-8 hours. So we tried 119 cycles of SUZI(normal; 39 cycles, subnormal; 80 cycles) using the preparation method of 6-8 hour incubation in 50% HFF. There were no signigicant differences in the fertilization rates between normal(125/269, 46.4%) and subnormal sperm(264/635, 41.6%). Contrary to the fertilization rates, pregnancy outcomes were different between both groups. Better results obtained from the subnormal group than the normal in the number of transferred embryos, that of good embryos, and developmental rate of the fertilized eggs. The pregnancy rates per transfer were totally 13.3%(13/98),20.0%(13/65) in subnormal group. In the normal group, 2 patients showed ${\beta}$-hCG positive, but resulted in chemical pregnancy. Of 13 clinical pregnancies, two aborted, 6 on-going, and 5 delivered. In conclusion, SUZI is an effective technique to overcome fertilization failure for male factor and unexplained. The fertilization rate is influenced by sperm parameters, sperm incubation media and time. Also the quality of oocytes might be important for pregnancy as same as that of sperm.
최규완(Kyoo Wan Choi),이종철(Jong Chul Rhee),장재권(Jae Kwon Jang),김재준(Jae J . Kim),이준행(Jun Haeng Lee),김영호(Yong Ho Kim),손희정(Hee Jung Son),이풍렬(Poong Lyul Rhee),백승운(Seung Woon Paik),오영륜(Young Lyun Oh) 대한소화기학회 2001 대한소화기학회지 Vol.38 No.1
Background/Aims: Telomerase activity has been detected not only in human cancers but also in precancerous lesion (e.g., intestinal metaplasia). It suggests that telomerase activation may be an early event in carcinigenesis, but it is not clear how telomerase is activited. Recently, Helicobacter pylori infection is known to be closely associated with intestinal metaplasia and gatric cancer. Thus, it is possibile that H. pylori infection may induce telomerase activation in intestinal metaplasia. In this study, we evaluated the relationship between telomerase activation and H. pylori infection. Methods: For 129 patients with gastric ulcer (n=20), chronic gastritis (n=34), gastric polyp (n=15), and intestinal metaplasia (n=60), telomerase activity was measured by Telomerase PCR ELISAR kit and H. pylori infection was determined by CLOR kit. Results: Telomerase activity was not detected in the patients with gastric ulcer, and chronic gastritis, gastric polyp, regardless of H. pylori infection. However, in 60 cases of intestinal metaplasia, telomerase activity was detected in 17 samples (28%). Among them, 12 samples were H. pylori positive, and the remaining 5 were H. pylori negative. The telomerase positivity was a little higher in the patients with H. pylori-positive intestinal metaplasia than in those with H. pylori-negative intestinal metaplasia, but the difference was not statistically significant (32% vs. 23% p=0.468). Conclusions: These results indicate that H. pylori infection can play a role partially, but is not the only cause of telomerase activation in intestinal metaplasia. (Korean J Gastroenterol 2001;38:15-22)
위장관 ( 胃腸管 ) : 만성 특발성 변비환자에 대한 알칼리성 이온수의 치료 효과
최규완(Kyoo Wan Choi),노임환(Im Hwan Roe),전무식(Mu Shik Jhon) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.4
N/A Calcium ion is considered to be the strong structure making ion, and also to be essential in the propulsive contraction of the intestinal smaoth muscle. Therefore we have experienced the therapeutic effect of the alkaline ionized water including large amount of calcium ion in the constipated patients. The effect of the alkaline ionized water in the constipated patients (n=8) was evaluated by the measurement of the colonic transit time using radiapaque markers and the frequency of defecation. The trensits were shortened significantly after treatment; from 71.8 +- 29.4 hr to 43.5 +- 26.5 hr in total conon (p=0.004), and from l8.8 +- 10.4 hr to 8.1 +- 6.2 hr in the right colon (p=0.035). The frequency of defecation was also inereased eignificantly: from 1.4 +- 0.6/week to 2.7 +- 1.6/week (p=0.03). ln conclusion, although the effect of the long term follow-up is not taken, this is a preliminary report showing the promising effects of the alkaline ionized water in the chronic constipated patients.
한국인 백혈병의 세포이전학적 연구 1. 만성 골수성 백혈병의 염색체 이상
최규완 ( Kyoo Wan Choi ),김동순 ( Dong Soon Kim ),이영복 ( Young Bock Lee ) 대한내과학회 1973 대한내과학회지 Vol.16 No.2
This is to report the result of the cytogenetic observation in 12 patients with chronic myelogenous leukemia who had been treated at the Seoul National University Hospital for the last one year. The age of the patients ranged from 16 to 34 years. The chrom
정상한국인의 염색체에 관한 연구 - 제 1 편 정상도시인의 염색체에 관한 연구
최규완 ( Kyoo Wan Choi ) 대한내과학회 1972 대한내과학회지 Vol.15 No.7
The purpose of this paper is to obtain the incidence of chromosomal aberration among the cells from normal, healthy Korean. The results of this study were compared with the data from the other countries and will serve as the control data for future studies