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Synthetic regulators for protein engineering
정규열,양진아,장성호,장성연 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
One of the most critical problems of protein engineering is the efficient screening method for the evolutionary approach. In this study, we developed synthetic selection devices using the expression regulators at transcription and translation levels. By combining the product-sensitive promoters and selection markers, a selection device turning the cellular phenotype into selective based on the intracellular concentration of the product. Additionally, intracellular metabolite sensor named “riboselector” as a RNA-based screening device will be presented. The potentials of the platform technology developed in this study for the application to the development of novel enzymes with high catalytic activities
Design of optimum platform chemical producer using fine expression controllers
정규열,장성연,임현규,장성호 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Pathway optimization of microbial metabolism is essential for the production of commercially valuable chemicals such as biofuels, platform chemicals and biologically active compounds. Currently, typical approach for metabolic engineering uses both rational approach as well as non-rational methods such as combinatorial and evolutionary methods. General strategy to modify cellular physiology depending the robustness or flexibility of the biological systems should be required. In this study, we developed the general tools to modify the biological robustness at the various levels including translation and protein levels. For translation level control, a model to predict the translation efficiency based on the mRNA’s secondary structure and consequently expression level can be precisely controllable for any genes of interest. Additionally, intracellular metabolite sensor named “riboselector” to regulate metabolic distribution will be presented. The potentials of the platform technology developed in this study for the application to the production of value-added biochemical including flavonoids and isoprenoids.