수중카메라(Underwater camera) 모니터링 기법을 이용한 인공보 건설에 대한 어류군집의 탁도 영향 : 예비실험

박향미(Hyang-Mi Park),최지웅(Ji-Woong Choi),임병진(Byung Jin Lim),안광국(Kwang-Guk An) 한국환경독성학회 2011 한국독성학회 심포지움 및 학술발표회 Vol.2011 No.10

애기땅빈대 추출물의 지방산 조성 및 인체 암세포 증식 억제 효과

최향미(Hyang Mi Choi),임선영(Sun Young Lim) 한국생명과학회 2014 생명과학회지 Vol.24 No.1

본 연구에서는 다양한 약효가 있다고 알려진 애기땅빈대(Euphorbia supina)의 추출물 및 분획물의 지방산 조성을 분석하고 인체 암세포 증식 억제효과에 대하여 살펴보았다. A+M과 MeOH 추출물의 지방산 조성 패턴은 유사했고 A+M과 MeOH 추출물은 각각 53.4% 및 42.1%의 18:3n-3를 함유하였다. 분획물들 중 85% aq. MeOH는 가장 높은 함량의 18:3n-3를 나타내었다. 애기땅빈대에 의한 암세포 증식 억제효과는 MeOH 추출물과 비교했을 때 A+M 추출물에 의한 억제효과가 높았으며 AGS 인체 위암세포에 대한 억제효과가 높았다. 분획물들 중에서는 n-Hexane과 85% aq. MeOH 분획물들에 의한 증식 억제효과가 높았다. 따라서 애기땅빈대의 항암 활성 성분은 n-Hexane과 85% aq. MeOH 분획물들에 함유되어 있는 것으로 여겨지며 향후 정제하여 규명할 필요가 있다고 사료된다. The objective of this study was to determine the fatty acid composition and the antiproliferative effect of extracts and fractions from Euphorbia supina. With regards the fatty acid composition, the percentages of 18:3n-3 in acetone/methylene chloride (A+M) and methanol (MeOH) extracts were 53.4 and 42.1%, respectively. Among the fractions, an 85% aqueous methanol (85% aq. MeOH) fraction contained the highest percentage of 18:3n-3. Treatments with crude extracts and fractions significantly inhibited the growth of HT-29 and AGS human cancer cell lines (p<0.05). The A+M extract showed a higher inhibitory effect on the growth of both cancer cells compared to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions exerted a greater inhibitory effect on the proliferation of both types of cancer cells. Our results suggest that 85% aq. MeOH and n-hexane fractions exert potent inhibitory effects on the proliferation of human cancer cells.

건조 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포 증식 억제효과

황성연(Seong Yeon Hwang),최향미(Hyang Mi Choi),임선영(Sun-Young Lim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.1

본 연구에서는 도라지를 건조한 후 분말화하여 유기용매로 추출하여 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포에 대한 증식 억제효과에 대해 검토하였다. 건조 도라지의 총 페놀계 화합물 함량 측정 실험에서 A+M 추출물과 MeOH 분획물은 각각 4.53 및 27.22 TAE mg/100 g의 페놀계 화합물 함량을 나타내었으며, 건조 도라지 분획물들 중 n-BuOH 분획물은 14.82 TAE mg/100 g로 가장 높은 페놀계 화합물 함량을 나타내었다. n-BuOH 분획물을 다시 RP flash column chromatography로 분획하여 얻은 분획물들(rfc 1-5) 중 rfc 1은 다른 분획물들보다 유의적으로 높은 페놀계 화합물 함량을(1.27 TAE mg/100 g) 나타냈다. 건조 도라지 추출물의 인체 암세포들(HT-29 및 AGS)에 대한 증식 억제효과 실험에서 A+M 추출물 및 MeOH 분획물은 인체 암세포 증식을 유의적으로 억제하였으며(p<0.05) 두 암세포에 대한 증식 억제효과에는 차이가 없었다. 건조 도라지 분획물들을 농도별로 처리하였을 때에도 n-BuOH 분획물의 IC<SUB>50</SUB>이 0.06 mg/mL로 가장 활성이 높았고 n-BuOH 분획물의 rfc 1-5를 암세포에 0.001-0.05 mg/mL의 농도로 처리했을 때 rfc 1-3의 경우, 0.005 mg/mL 이상의 농도에서 높은 암세포 증식 억제효과를 나타내었다. 이상의 연구결과로부터 건조 도라지의 n-BuOH 분획물에 의한 강력한 암세포 증식 억제효과는 높은 함량의 페놀계 화합물에 기인한 것으로 여겨지며 향후 분획물의 분리 정제를 통한 새로운 기능성 물질의 개발이 필요할 것으로 사료된다. We investigated the effects of extracts from dried Platycodon grandiflorum on total phenolic content and growth of cancer cell lines (HT-29 and AGS). Total phenolic contents of acetone/methylene chloride (A+M) extract and methanol (MeOH) fraction were 4.53 and 27.22 TAE mg/100 g, respectively. Among the fractions, n-butanol (n-BuOH) fraction contained the highest phenolic content. Treatments of crude extracts and fractions significantly inhibited the growth of HT-29 and AGS cancer cell lines (p<0.05). Among the fractions, n-BuOH fraction exhibited the highest inhibitory effect and was then sub-fractionated by reverse phase flash column chromatography (rfc). The rfc 1-3 exhibited a higher inhibitory effect on proliferation of both cancer cells. The rfc 1 contained the highest phenol content. Our results showed that n-BuOH fraction possessed a potent inhibitory effect on proliferation of human cancer cells. We suggested that this anticancer activity was partially related to the content of phenolic compounds.

델파이 기법을 활용한 사회인 야구 활성화 방안 연구

최유미(Choi, You-Mi),임향미(Lim, Hyang-Mee),이상일(Lee, Sang-Il) 한국사회체육학회 2016 한국사회체육학회지 Vol.0 No.66

The purposes of this study are to examine the problems and improvements of the current recreational baseball leagues and propose a measure to vitalize the recreational baseball leagues through delphi technique in order to propose an integrated measure by collecting the opinions of experts. Delphi survey was conducted for a total of 4 times. In regard to the collected results, the factors were categorized through the expert meeting. As a result, 4 final meta-factors and 20 sub-factors were derived. As a result of the final delphi survey, ‘expansion of relevant management departments for recreational baseball leagues’ and ‘mitigation of permit system for baseball facility’ were found to have the highest degree of potential for change in relation to the administrative system. As for hope scale, ‘expansion of relevant management departments for recreational baseball leagues’ was found to have the highest degree. In regard to organization, ‘improvement of the problem associated with league operation and political adhesion relationship’ was found to have the highest degree of potential for change. As for hope scale, ‘integration of different local rules of each league’ was found to have the highest degree. In regard to facility and supplies, ‘new construction of baseball park in each region’ was found to have the highest degree of potential for change. As for hope scale, ‘distribution of diverse supplies and equipment’ was found to have the highest degree. Lastly, in regard to infrastructure, ‘securing budget through corporate sponsorship’ was found to have the highest degree of potential for change. As for hope scale, ‘promotion of recreational baseball leagues in local communities’ were found to have the highest degree.

생활체육 당구 참가자의 참여동기, 몰입경험 및 운동지속의사와의 관계

이상일(Lee, Sang-Il),임향미(Lim, Hyang-Mee),황인선(Hwang, In-Sun) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.4

The purpose of this study is to demonstrate why people participate billiards which is one of the sports for all programs and what makes them re-participate, in addition to analyze the relationship between the immersion experience and re-participation volition so as to provide basic data for the vitalization of billiards. The conclusions from the research by a correlation analysis, multiple regression analysis are as follows: First, billiard march participates" motivation has an effect on inner pleasure of immersion cognition and behavior and outer condition. Second, billiard march participates" motivation has an effect on habitual exercising, and inner pleasure, outer condition and outer ostentation in environmental factors. Third, billiard march participates" immersion experience has an effect on immersion cognition and behavior cognition of habitual exercising, and immersion behavior of continuing exercising environment. Conclusively, we need to switch our thinking billiard as one of the sound leisure programs and develop step by step programs for the interest and new skills. Also persistent administrating and special regard to facilities by providing relevant are necessary for the persistent participation.

참치 추출물의 일산화질소 및 사이토카인 생성에 미치는 영향

김광혁(Kwang-Hyuk Kim),최명원(Myoung Won Choi),최향미(Hyang Mi Choi),임선영(Sun-Young Lim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3

The effect of tuna extracts on the production of nitric oxide (NO) and cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ), was investigated. All extracts and fractions from tuna significantly reduced NO production induced by lipopolysaccharide (LPS). The acetone+methylene chloride (A+M) extract, n-hexane and 85% aqueous methanol (MeOH) fractions had stronger inhibitory effects among them. The 85% aqueous MeOH fraction at a 10-㎍ concentration significantly decreased LPS-induced IL-6 and TNF-α productions at 6 h of incubation. In the case of LPS-induced IFN-γ production, the 85% aqueous MeOH fraction at a 3-㎍ concentration showed significantly higher levels at 48 h of incubation. These results show that the 85% aqueous MeOH fraction inhibited the production of NO and pro-inflammatory cytokines (IL-6, TNF-α), suggesting that this fraction acts as a potent immunomodulator.

상업스포츠센터 지도자 리더십 유형이 선호도 및 재이용에 미치는 영향

이상일(Sang Il Lee),유현순(Hyun Soon Yu),강윤남(Yun Nam Kang),임향미(Hyang Mee Lim) 한국사회체육학회 2011 한국사회체육학회지 Vol.0 No.44

The purpose of this study was to analyze how the leadership type of leaders working at commercial sports centers affects leader preferences and reutilization. To attain the purpose, questionnaire surveys were conducted for 395 members in three commercial sports centers located in Gyeonggi Province. Results of the survey were as follows: First, among the leaders working at the commercial sports centers, charisma and intellectual stimulation which are factors of the transforming leadership and management by exception which is a factor of the transactional leadership appeared to significantly affect when preferring a leader. Second, charisma and intellectual stimulation among factors of the transforming leadership and management by exception among those of the transactional leadership showed significant influence upon the reutilization. Third, the preferences of members for leader appeared to exert significant influence upon the reutilization. In conclusion, members of the commercial sports centers were affected by the leadership type of their leaders which would bring forth a favorable feeling or vice versa. Since such preference upon leaders is an important factor in subscribing the sports center once again, its importance shall not be underestimated. Thus, to get competitive advantages in fierce competitions, a manager of a commercial sports center should take much interest in leaders and provide appropriate leadership programs and workshops with leaders who have made close relationships with members. On the other hand, as a leader is obliged to provide high quality physical training programs and services to his/her members, he/she has to develop and demonstrate his/her leadership competence including charisma, intellectual stimulation, and management by exception to be a leader preferred by members.

Echo-sounder 기법을 통한 인공보 건설에 대한 서식지 변화 및 어류 영향분석

한정호(Jeong-Ho Han),곽상도(Sang-Do Kwak),박향미(Hyang-Mi Park),임병진(Byung Jin Lim),안광국(Kwang-Guk An) 한국환경독성학회 2011 한국독성학회 심포지움 및 학술발표회 Vol.2011 No.10

Lipopolysaccharide (LPS)에 의해 유도된 염증에 대한 건조 고등어 추출물의 항염증 효과

김광혁(Kwang-Hyuk Kim),최명원(Myoung Won Choi),최향미(Hyang Mi Choi),임선영(Sun-Young Lim) 한국생명과학회 2013 생명과학회지 Vol.23 No.9

본 연구에서는 건조 고등어 추출물 및 분획물들에 의한 nitric oxide 생성에 미치는 영향을 살펴보았고 건조 고등어 85% aq. MeOH 분획물을 중심으로 면역과정의 생물학적 작용과 대사적 변화를 유도하는 IL-6, IFN-γ 및 TNF-α 같은 pro-inflammatory 사이토카인의 생성을 측정하여 건조 고등어 추출물에 의한 항염증 효과에 대하여 검토하였다. 건조 고등어 추출물과 각 분획물은 control보다 낮은 nitric oxide 생성량을 나타내었으며, 특히 85% aq. MeOH 및 n-hexane 분획물에 의한 저해효과가 높았다. 건조 고등어 분획물은 Con-A 보다는 LPS에 의해 자극된 IL-6, TNF-α 및 IFN-γ의 생성을 감소시키는데 더 효과적이었다. IL-6 및 TNF-α의 생성은 배양시간 6시간 후 건조 고등어 85% aq. MeOH 분획물의 모든 첨가농도(1, 3 및 10 μg)에서 감소되었다(p<0.05). IFN-γ의 경우, 건조 고등어 85% aq. MeOH 분획물을 LPS와 함께 처리했을 때 특히 72시간 배양 IFN-γ의 생성량이 농도 의존적으로 감소하였고 Con-A에 의해 자극된 IFN-γ의 생성량은 6 및 24시간 배양 후 유의적으로 감소하였다(p<0.05). 이상의 결과로부터 건조 고등어 85% aq. MeOH 분획물은 nitric oxide 생성과 pro-inflammatory 사이토카인(IL-6, TNF-α, and IFN-γ)을 감소시켜 염증반응을 예방할 것으로 기대된다. The effect of dried mackerel extract on the production of nitric oxide (NO) and cytokines, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ), was investigated. All extracts and fractions from dried mackerel significantly reduced NO production induced by lipopolysaccharide (LPS). Among the extracts, acetone+methylene chloride (A+M), n-hexane, and 85% aqueous methanol (MeOH) showed the strongest inhibitory effects. The 85% aqueous MeOH fraction at a concentration of 10 μg significantly decreased LPS-induced IL-6 and TNF-α production after 6 hr of incubation. In the case of LPS-induced IFN-γ production, the 85% aqueous MeOH fraction decreased the production of IFN-γ afer 6, 24, and 72 hr of incubation in a dose-dependent manner. The results show that an 85% aqueous MeOH fraction inhibits the production of NO and proinflammatory cytokines (IL-6, TNF-α, IFN-γ), suggesting that this fraction acts as a potent immunomodulator.

직업무용단의 내부마케팅이 조직몰입 및 직무만족에 미치는 영향

박영혜(Park, Yong-Hye),황인선(Hwang, In-Sun),임향미(Lim, Hyang-Mee),이상일(Lee, Sang-Il) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.1

This research studied the effect of the internal marketing of dance company on organizational commitment and job satisfaction and use the results to provide information useful for operation of dance company in order to enhance service quality to improve customer satisfaction. A questionnaire survey was conducted on the members of dance company working for local dance companies in the region. The survey was conducted on a total of 376 people to collect data on demographic characteristics, internal marketing, organizational commitment and job satisfaction. A total of 34 surveys were omitted from the sample and 342 samples were used to perform frequency analysis, factor analysis, reliability analysis,, correlations analysis and multi-variate regression analysis. The following conclusions were obtained as a result of above research method and analysis. First, internal marketing had significant influence on organizational commitment. Second, internal marketing significantly affected job satisfaction. Third, organizational commitment significantly affected job satisfaction. In conclusion, the research showed that internal marketing of dance company affected organizational commitment and job satisfaction. The results suggest that there is a need for reinforcing internal marketing strategy for dancers in order to improve customer satisfaction and service quality. Therefore, dance companies are encouraged to continue to motivate their dancers and provide useful information to build a better working environment.