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카트리지 전처리를 이용한 절연유 중 PCBs 동족체의 정확도 평가
임태숙 ( Tae Sook Lim ),정인영 ( In Young Chung ),김지인 ( Ji In Kim ),고상호 ( Sang Ho Go ),이원석 ( Won Seok Lee ) 한국환경분석학회 2013 환경분석과 독성보건 Vol.16 No.3
Ulsan, a representative industrial city in South Korea, has various sources of heavy metals. The upstream of the Taehwa River in Ulsan is used to supply drinking water, but heavy metal pollution in this area has not been studied yet. Therefore, in this study, we investigated the levels and patterns of heavy metals in the Taehwa River including the upstream. We also evaluated water quality using the water pollution index. We collected water samples at 18 sites of the Taehwa River in February and May 2011. Target heavy metals were cadmium,chromium, lead, nickel, and zinc, which were analyzed by an inductively coupled plasma mass spectrometer (ICP-MS). The concentration of heavy metals increased from the upstream to downstream, and the highest concentration was detected in the downstream nearby an industrial complex. The spatial distribution of heavy metals and water pollution indexes indicated that some urban sites and most industrial sites were artificially polluted, and non-point sources might play an important role in water pollution of the Taehwa River.
표면개질된 영가철 나노입자를 이용한 질산성 질소 제거율 향상에 대한 연구
임태숙 ( Taesook Lim ),조윤철 ( Yunchul Cho ),조장환 ( Changhwan Cho ),최상일 ( Sangil Choi ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.4
In order to treat groundwater containing high levels of nitrate, nitrate reduction by nano sized zero-valent iron (nZVI) was studied using batch experiments. Compared to nitrate removal efficiencies at different mass ratios of nitrate/Fe0, the removal efficiency at the mass ratio of 0.02% was the highest(54.59%). To enhance nitrate removal efficiency, surface modification of nZVI was performed using metallic catalysis such as Pd, Ni and Cu. Nitrate removal efficiency by Cu-nZVI (at catalyst/Fe0 mass ratio of 0.1%) was 66.34%. It showed that the removal efficiency of Cu-nZVI was greater than that of the other catalysts. The observed rate constant (kobs) of nitrate reduction by Cu-nZVI was estimated to 0.7501 min-1 at the Cu/Fe mass ratio of 0.1%. On the other hand, TEM images showed that the average particle sizes of synthetic nZVI and Cu-nZVI were 40~60 and 80~100 nm, respectively. The results imply that catalyst effects may be more important than particle size effects in the enhancement of nitrate reduction by nZVI.
혈중 변역 복합체 검출을 위한 Raji Cell ELISA법 의 개발
임태숙 ( T S Rim ),이은미 ( E M Lee ) 대한임상검사과학회 1991 대한임상검사과학회지(KJCLS) Vol.23 No.1
There are various kinds of circulating immune complexes(cic) and we have a number of methods of measuring them. However, any of them can not alone measure cic correctly, usually two or three methods are combined to be applied. Currently, in the immunological laboratory, the solid-phase Clq ELISA (Enzyme Linked Immuno -sorbent Assay) which uses a component of the complement system(C1q) is commonly used, but in an effort to more correctly measure cic, another kind of ELISA using Raji cell was experimented. To be more specific, optimum conditions were put forward synthesizing the reaction factors of the Raji cell ELISA and based on those conditions, cics of the autoimmune disease patients and the normal control group were measured with the three methods (Raji cell ELISA, Raji cell RIA and Clq ELISA). Following results were obtained. 1. The optimum conditions of the Raji cell ELISA which displayed the best standard curve were ; 1 : 4 dilution ratio of AHGG. 1 : 4 dilution ratio of NHS, 2 X l06cells/ml of Raji cell numbersㆍ 1 : 1, 000 dilution ratio of conjugate, 20minutes of substrate (PNP) reaction time and 4ㆍc reaction temperature of sample and Raji cells. 2. The coefficient (C. V.) variation of interassays of the Raji cell ELISA was 13.19%, which that of intraassay was 5.16%. 3. For the normal control group the mean of cic levels was 10.3±5.0(mean±S.D),ug of AHGG equivalent/ml with the Raji cell RIA and 11. 7±5. O,ug of AHGG equivalent/ml respectively. 4. For the autoimmune disease patient group, the mean of cic levels was 89. 3 ± 105 .l(mean ± S. D.) ,ug of AHGG equivalent/ml with. the solid -phase C1q ELISA, 118.5±140.0,ug of AHGG equivalent/ml with the Raji cell RIA and 195. 5± 257. 5,ug of AHGG equivalent/ml with the Raji cell ELISA, all of which showed higher levels of cics than the normal control group significantly. 5. It was also revealed that correlation between the solid-phase Clq ELISA and Raji cell ELISA was 0.34(P<0.0001), while the correlation between the solid-phase C q ELISA and the Raji cell RIA and between the Raji cell RIA and the Raji cell ELISA were 0. 49 (P < 0. 0001) and 0. 63 (P < 0. 0001) respectively. Summing up, the optimum condition of Raji cell ELISA is established, and in order to more correctly measure the cic levels, the principle of measurement and the sensitivity of the tests should be considered. In this regard, the combined use of the solid-phase Clq ELISA and the newly-experi Raji cell ELISA is believed to be more effective than single C1 q ELISA.
주거환경 중 다환방향족탄화수소(PAHs) 분석방법 제시를 위한 연구
고상호,임태숙 한국환경분석학회 2019 환경분석과 독성보건 Vol.22 No.4
In this study, methods of extraction, purification, and instrumental analysis are validated for 16 types of polycyclic aromatic hydrocarbons (PAHs) defined by the United States Environmental Protection Agency (US EPA), to analyze their occurrence during cooking in residential environments. Accelerated solvent extraction (ASE), which is more efficient than other extraction methods, are used and the recovery rate of gaseous samples (excluding NaP) and particulate samples was 80-110% with a relative standard deviation (RSD) of 0.6-19.8%. The purification method was compared with the column and the cartridge purification, with recovery rates of 80-110% and 60-100%, respectively. Through this process, a high recovery rate of the 16 substances was confirmed by the column purification method after ASE. The rate was 78-102% (RSD: 0.3-10%) for the gas samples and 73.7-101.7% (RSD: 0.7-20%) for particulate samples, respectively. The recovery rate of the analytical method used in this study was 66.6-80.2% (RSD: 4.7-8.3%) in the gas phase samples and 72.5-97% (RSD: 5.1-10.1%) in the particulate samples. As a result, it seems reasonable to apply the analytical method proposed in this study to real sample analyses.