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임미경,송정모,Im, Mi-Kyung,Song, Jung-Mo 사상체질의학회 2008 사상체질의학회지 Vol.20 No.3
1. Objectives The purpose of this case study is to evaluate the effects of Sasangconstitutional diagnosis and treatment of chronic idiopathic urticaria patient who was diagnosed Taeeumin Dry-Heat Syndrome(煥熱證). 2. Methods Chronic idiopathic urticaria patient was diagnosed Taeeumin Dry-Heat Syndrome(煥熱證) based on their Nature & Emotion(性情), physical characteristics, symptoms. She was medicated Cheongsimyeonjatang(淸心蓮子湯). 3. Results and Conclusions Chronic idiopathic urticaria patient who was treated with Cheongsimyeonjatang(淸心蓮子湯) showed improvement in urticaria symptom and general condition. This case study describe the effectiveness on Chronic idiopathic urticaria symptom by using Cheongsimyeonjang(淸心蓮子湯).
<sup>51</sup>Cr방출과 MTT 및 LD활성도를 이용한 역충전재의 세포독성에 관한 연구
최라영,임미경,Choi, La-Young,Im, Mi-Kyung 대한치과보존학회 1994 Restorative Dentistry & Endodontics Vol.19 No.2
Endodontic surgery is performed when conventional endodontic therapy fails or is contraindicated. In such cases, retrograde filling materials including amalgam, composite resin, and various cements have been used. Biocompatibilty and margin sealing ability of retrograde filling materials are important for the long term success of endodontic surgery. In vitro cell culture is frequently used as the method of measuring the biocompatibilty of dental materials. The purpose of this study was to evaluate the cytotoxicity of six kinds of retrograde filling materials including newly developed light curing glass ionomer cements. Each material was mixed according to. the manufacture's instruction and evaluated as : freshly mixed, 24-hour after mixing, and 168-hour after mixing respectively. The elution solution was extracted after 24-hour contact with materials using media. Cytotoxicity was evaluated by direct contact, or elution contact. Test results of radiochromium($^{51}Cr$) release, cell viability using tetrazolium dye (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl dimethyltetrazolium bromide(MTT) test and lactate dehydrogenase(LD) of damaged L929 cells were analyzed. In the $^{51}Cr$ release of direct contact, all experimental retrograde filling materials except amalgam and glass ionomer cement showed increased cytotoxicity compared to control. In the $^{51}Cr$ release of elution solution, the released $^{51}Cr$ was so minimal that it was impossible. to evlauate the cytotoxicity exactly. The elution solutions of glass ionomer cement and IRM showed marked cytotoxicity in MTT test. LD enzyme activity was highest in tests of direct contact with composite, light curing composite, and light curing glass ionomer cement and IRM. Amalgam revealed least cytotoxicity while IRM showed cytotoxicity using all three methods. Composite, light curing composite and light curing glass iomomer cement were cytotoxic in the tests of $^{51}Cr$ release and LD activity. Glass ionomer cement showed cytotoxic effect only in the MTT method. From these results it is suggested that the standardization and optimization of cytotoxicity testing, especially using elution solutions, should be strongly advised.
레진 배양액의 생체내 및 실험실 세포독성에 미치는 영향
임미경,김은철,유수경,김강주 원광대학교 생체재료·매식연구소 1992 원광생체재료·매식 Vol.2 No.1
To know the in vitro and the in vivo cytotoxicity of resin solution, resin solution was applied to cultured fibroblast and was injected into the mouse. The cytotoxic effect of resin solution was measured by MTT assay and in vivo cytotoxicity was examined after Hematoxylin and Eosin staining. The cell activity of resin solution in the concentration of 59% was significantly decreased compared to control group and 5% group. In histopathologic study of resin solution, there were severe inflammatory cell infiltration, mild interstitial edema, trace hemorrhage, and moderate or severe muscle destruction in resin injected group. These results suggested that there might be some differences between the cel viability of fibroblast and in vivo cell cytotoxicity. Further study is needed to clarify the cytotoxicity by direct implanting of resin mass.
임미경,정희일,오세홍 원광대학교 생체재료·매식연구소 1995 원광생체재료·매식 Vol.4 No.1
Balance between antibacterial effectiveness and biocompatibility is important property of root canal irrigation solutions. The aim of this study was to evaluate the cytotoxic effect of irrigation solutions on cultured L929 cells. Seven irrigation solutions and thier serial dilution solution were applied to L929 cells. Radiochromium release of damaged L929 cells after five-minute, thirty-minute and four-hours exposure to each irrigation solutions were monitored using gamma particle counter. L929 cells which were exposed to 1:1 and 1:10 diluted solutions of sodium hypochlorite and cresol for five and thirty minutes showed cytotoxic change. Povidone had cytotoxic effect on L929 cells after five minutes, thirty minutes and four hours. Normal saline, alcohol, Wydex and hydrogen peroxide showed weak cytotoxic effect on L929 cells.
광중합형 복합레진과 콤포머에서 두께와 배경색에 따른 색변화
임주환,임미경 원광대학교 생체재료·매식연구소 1999 원광생체재료·매식 Vol.8 No.1
The color of an esthetic restorative material is controlled primarily by thickness of the material and background color. Although the effects of the two factors on the color coordinates of esthetic dental materials have been reported, the mechanism has not been clarified well enough to explain the effects quantitatively. The purpose of this study was to evaluate the effect of thickness and background color on the color of tooth colored restorative materials quantitatively. One hundred sixty samples were fabricated from two commercial light-cured composite resins and two commercial compomer and the shade was Vita A3. The color characteristics and changes in the color coordinates were measured by a tristmulus colorimeter (Model TC-6FX, Tokyo Denshoku Co. Japan) using the CIELAB system. The results were as follows: 1. As thickness increased from 1.0 to 4.0mm, values of L^* a^* b^* changed irregulary for white and dentin color background, but showed no obvious difference in color for black background. 2. The colors of composite resins and compomers were significantly influenced by background color. 3. The color difference was recognized even the same shade name in four representative kinds of composite resins and compomers. 4. As thickness changed, values of color difference for same products and same background color showed constancy but showed difference for different background color.
이용근,이건일,임미경 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.2
The purpose of this study was to find correlations among the color differences measured by three methods. Those were 1) spectrophotometric method(Differential Colorimeter, Model TC-6FX, Denshoku, Japan), 2) digital image method-photographed by digital camera(KODAK Digital Camera 40, Kodak, U.S.A.) and processed by computer program(Adobe Photoshop 3.0), 3) 35 mm standard slide method-took photographic color slide, read using scanner(Nikon 35 mm Film scanner, LS-3510 AF, Nikon, Japan) and processed by computer program(Adobe Photoshop 3.0). The color of resin modified glass ionomers were determined in vitro before and after thermocycling for 500 to 2,000 cycles. The values of CIELAB ΔE*, L*, a*, b*, r, g, b, r*, g*,b* were analyzed to find correlations among them. From the experiment, the following results were obtained: 1. There were no statistically significant correlation among the values of CIELAB ΔE*, ΔC measured by digital camera and ΔC* measured by color slide and scanner (Sig. F>0.05). 2. There was a statistically significant correlation between the values of CIELAB L* and GR measured by digital camera(Sig. F=0), and some pairs showed significant correlation among CIELAB a*, b* value and r, g, b value determined by digital camera and r*, g*, b* value determined by color slide and scanner. 3. The values of r, g, b determined by digital camera, and the values of r*, g*, b* value determined by color slide and scanner showed significant correlation between each corresponding values (Sig. F=0). In this case, correlation coeffcient were 0.339-0.413. 4. In the same materials, the degree of color was different depending on the shades, and of some shades showed of materials clearly different color change depending the measuring method.