소음병(少陰病) 부자탕(附子湯)으로 호전된 만성 피로 증후군을 동반한 대상포진 후 신경통 1례

이자복,Lee, Ja-Bok 대한상한금궤의학회 2015 대한상한금궤의학회지 Vol.7 No.1

Objective : This study aims to explain the effect of Buja-tang on Postherpetic Neuralgia with Chronic Fatigue Syndrome which a Soeum-byeong patient has. Methods : This is a case report on a female patient who has been suffering from Postherpetic Neuralgia with Chronic Fatigue Syndrome. According to Diagnostic system based on Shanghanlun provisions, Buja-tang was provided for one month. Results : After a series of Buja-tang treatments, the patient's symptoms and result improved. Conclusions : This case study show an effectiveness of using Buja-tang according to Diagnostic system based on Shanghanlun provisions on Postherpetic Neuralgia with Chronic Fatigue Syndrome.

락토바실러스 람노서스(Lactobacillus rhamnosus) KCTC 5033의 Probiotics와 Paprobiotics 섭취에 의한 얼굴미용효과

이자복 ( Ja Bok Lee ),석지한 ( Ji Han Suk ),강상모 ( Sang Mo Kang ) 대한미용학회(구 대한미용과학회) 2018 대한미용학회지 Vol.14 No.3

Lactic acid bacteria are often taken in the form of yogurt-which is becoming a highly stable food type often consumed daily-or medicine. Lactic acid bacteria have a significant industrial value because of the various reports of health improvement effects that they may convey (eg, anti-microbial, improved immunity). This study examined changes in facial skin when middle-aged women (ie, 40~50s) took probiotic and para-probiotic medicines derived from Lactobacillus rhamnosus KCTC 5033. The research subjects were 30 middle-age women who were randomly assigned (10 per group) to consume either: i) probiotics of L. rhamnosus KCTC 5033, ii) bacteria-killing paraprobiotics, or, iii) a control. Each group consumed 1 capsule (1×1010 CFU or more) of assigned treatment on an empty stomach every morning for 12 weeks, and changes in their facial and neck skin before and after consumption were observed. Their skin change, melanin index, erythema index, moisture content, TEWL, skin roughness, and skin surface temperature were measured to identify any potential differences. A survey was also used to investigate respondent’s satisfaction with their skin before and after lactic acid bacteria intake on a 5-point Likert scale. Before and after results demonstrated that melanin index significantly increased in only the control group while moisture content increased significantly in the probiotics and paraprobiotics groups. Also in the probiotics and parabiotics groups, moisture content increased, while skin roughness decreased. Also in the probiotics and parabiotics groups: i) moisture content increased, ii) skin roughness decreased, and iii) no changes (before to after) in erythema index, TEWL or skin surface temperature were observed. The results of probiotics and paraprobiotics on neck skin indicated that the control group experienced a significant increase in melanin index, skin roughness, and TEWL; moisture content increased significantly in the probiotics and paraprobiotics groups, and skin surface temperature increased significantly in only the probiotics group.

락토바실러스 람노서스(Lactobacillus rhamnosus) KCTC 5033제제 섭취가 40-50대 중년여성의 목피부에 미치는 영향

이자복 ( Ja Bok Lee ),전영현 ( Young Hyun Jeon ),강상모 ( Sang Mo Kang ) 한국미용학회 2018 한국미용학회지 Vol.24 No.4

This study investigated changes in neck skin appearance middle-aged women in their 40s and 50s after ingesting probiotic or paraprobiotic Lactobacillus rhamnosus KCTC 5033. Women in each group consumed one capsule (1 × 10¹⁰CFU) for 3 months and underwent measurements of changes in neck skin appearance. Melanin content was significantly increased only in the placebo control group, whereas the moisture content was not altered in either the probiotic or paraprobiotic group. in the probiotic group. The erythema index was not changed, but oil content and roughness tended to increased oil content and decreased roughness in the probiotic and paraprobiotic groups compared with the findings prior to the experiment. Moisture was increased in both the probiotic and paraprobiotic groups, whereas moisture evaporation was significantly increased only in the control group. The neck skin surface temperature was only changed in the probiotic group. Therefore, the probiotic and paraprobiotic treatments inhibited melanin production by altering the intestinal condition, in addition to increasing moisture by increasing the oil content and decreasing skin roughness. In fact, subjects in these groups expressed greater satisfaction after the experiment, especially those in the paraprobiotic group. Thus, L. rhamnosushas been identified asa potential therapeutic for improving the appearance of skin for the first time.

양명병(陽明病) 인진호탕(茵蔯蒿湯)으로 호전된 유두 습진 1례

이성준,이자복,Lee, Sung-Jun,Lee, Ja-Bok 대한상한금궤의학회 2014 대한상한금궤의학회지 Vol.6 No.1

Objective : This study aims to explain the effect of Injinho-tang on Eczema which a Yangmyung-byeong patient has. Methods : This is a case report on a female patient who has been suffering from Eczema. According to Diagnostic system based on Shanghanlun provisions, Injinho-tang was provided for six months. Results : After a series of Injinho-tang treatments, the patient's symptoms and result improved. Conclusions : This case study showed an effectiveness of using Injinho-tang according to Diagnostic system based on Shanghanlun provisions on Eczema.

『상한론(傷寒論)』 대양병(大陽病) 96조(條)에 근거한 소시호탕(小柴胡湯)을 투여하여 호전된 욕지기 치험 1례

이래춘,이자복,Lee, Lae-Chun,Lee, Ja-Bok 대한상한금궤의학회 2013 대한상한금궤의학회지 Vol.5 No.1

Objective : This study aims to explain the effect of Soshiho-tang on an nausea which a Taeyangbyeong patient has. Methods : This is a case report on a male patient who has been suffering from nausea which is accompained by anger. According to Diagnostic system based on Shanghanlun provisions, Soshiho-tang was provided for four weeks. Results : After a series of Soshiho-tang treatments, the patient's symptoms and result improved. Conclusions : This case study showed an effectiveness of using Soshiho-tang according to Diagnostic system based on Shanghanlun provisions on nausea.

고지방식이로 유도된 C57BL/6 mice에서 감국이 미치는 비만억제 효과

최재영,이자복,김명옥,Choi, Jae Young,Lee, Ja-bok,Kim, Myeong-ok 중소기업융합학회 2021 융합정보논문지 Vol.11 No.4

감국의 유산균 발효를 통한 감국 배양물(CILL)의 비만억제 식품의 소재로서 가능성을 알아보기 위해 고지방식이로 유도된 C57BL/6 mice에서의 체중, body fat mass, T cell 등을 측정하였다. 체중 변화에서 CILL 군 (25.15±2.44 g)은 4주부터 체중 감소를 보였으며, 1주차부터 8주차까지 낮은 증체량을 유지했다(1.00±0.53 g). 8주 체중(30.38±4.17 g)은 HFD 군(60% high fat diet, 34.99±2.09 g) 대비 13.15%의 체중 감소를 보였다. Fat mass는 10.3022±2.8813 g로 감소했으며, 간 절대 중량에서 HFD 군에 비해 감소하였다. CD4+ T cell 4.84±1.33%, CD8+ T cell 7.02±2.26%, CD4+CD8+ T cell 1.46±0.81%로 HFD 군에 비해 높게 측정되었다. 결과적으로 CILL은 비만억제 효과가 있으며, CILL내의 비만억제 물질을 선별한다면 효과적인 비만억제 식품 소재로서의 가능성이 있을 것으로 사료된다. In order to determine the possibility that Chrysanthemum indicum L. cultured with Lactococcus lactis (CILL) is a material for obesity suppression food, the body weight, body fat mass, and T cells were determined in C57BL/6 mice induced by a high fat diet. The CILL (25.15±2.44 g) demonstrated weight loss from week 4 onward and maintained a low weight gain from week 1 to week 8 (1.00±0.53 g). The 8-week body weight (30.38±4.17 g) indicated loss of 13.15% when compared to the HFD (60% high fat diet, 34.99±2.09 g). Fat mass decreased to 10.3022±2.8813 g, and the absolute liver weight decreased relative to that in the HFD. CD4+ T cells were 4.84±1.33%, CD8+ T cells 7.02±2.26%, and CD4+CD8+ T cells 1.46±0.81%, which were all higher than those in the HFD. As a result, CILL can be used as a material for preventing obesity as an effective measure toward reducing weight when consumed orally.

김치 유래 lactic acid bacteria의 heat-killed cell 항산화능 평가

조영재 ( Young-jae Cho ),이자복 ( Ja-bok Lee ),강상모 ( Sang-mo Kang ) 한국미용학회 2021 한국미용학회지 Vol.27 No.1

To use kimchi lactic acid bacteria as a cosmetic ingredient, screening was performed. The antioxidant activity of the heat-killed lactic acid bacteria isolated from kimchi was measured. Of the 134 strains of lactic acid bacteria derived from kimchi, 26 strains were selected based on the culture suitability such as growth rate and acid resistance. DPPH assay, ABTS assay, FRAP assay, nitrite radical scavenging activity assay, and total phenolic contents were measured for 26 strains, and with Lactobacillus rhamnosus KCTC 5033 as a reference, 5 strains were selected through DPPH assay, 2 strains through ABTS assay, 13 strains through FRAP assay, 5 strains through nitrite radical scavenging activity assay, and 10 strains through total phenolic contents measurement. Among them, Lactobacillus sp. 7-2, which showed excellent antioxidant potential in all experimental methods was isolated, and as a result of identification through 16s rRNA, it was found as Lactobacillus paracasei. This strain showed 1.09-1.90 times superior antioxidant potential than L. rhamnosus KCTC 5033, which is widely used for research purposes according to the method of measuring antioxidant potential. Thus, from kimchi, it was possible to isolate lactic acid bacteria which can be used as antioxidants in cosmetics.

홍만병초 분획물에 의한 HT-29대장암 세포의 Wnt/β-catenin 신호전달 조절

심보람(Bo Ram Sim),남영선(Young Sun Nam),이자복(Ja Bok Lee) 한국생명과학회 2019 생명과학회지 Vol.29 No.8

본 연구에서는 Rhododendron brachycarpum (RB, 홍만병초)의 80% 메탄올 추출물 및 분획물의 항암 활성을 규명하고자 하였다. RB n-hexane 분획물은 HT-29 세포에서 가장 높은 활성 저해를 보였다(IC50 = 20.2±1.2 μg/ml). 더욱이, 콜로니와 구형 형성은 수와 크기는 유의적으로 감소시켰다. RB의 n-hexane 분획물에서(0.22±0.02 fold change) TOP / FOP 플래시 리포터 억제 활성은 추출물 및 다른 분획물 보다 낮게 나타났다. n-hexane 및 ethyl acetate 분획물은 세포 내 β-catenin의 발현을 조절하였다. 2 차 대사 산물이 β-catenin 분해를 감소시킬 수 있는지 여부를 조사하기 위해 Western blot을 실시한 결과 n-hexane 분획물에서 p-GSK3β를 조절하였으며, 세포내 β-catenin은 핵에서 정량적인 변화를 가져왔다. 이러한 결과는 RB의 n-hexane 분획물로부터 천연 항암 물질을 포함하고 있음을 보여줍니다. The aim of this study was to determine the anticancer activities of an 80% methanol extract and various fractions of Rhododendron brachycarpum (RB). The n-hexane fraction of RB showed the highest inhibitory activity (Inhibit concentration 50% = 20.2±1.2 μg/ml) in HT-29 cells. Colony- and sphere-forming abilities were significantly correlated with a decrease in the cell count and size. A TOP/FOP flash reporter assay revealed that the inhibitory activity of the n-hexane fraction of RB (0.22±0.02 fold change) was lower than that of the 80% methanol extract and that of other fractions. The n-hexane and ethyl acetate fractions of RB were predominantly dependent on the expression levels of intracellular β-catenin. Western blotting using p-GSK3β with only the n-hexane fraction of RB was conducted to examine whether these secondary metabolites reduced β-catenin degradation. Intracellular β-catenin regulation resulted in quantitative changes in the nucleus. In summary, these results demonstrate the potential of the n-hexane fraction of RB as a natural anticancer agent.

Camphor의 Hedgehog 신호 SMO 조절을 통한 지방구세포 분화 억제효과

최재영(Jae Young Choi),임종석(Jong Seok Lim),이자복(Ja Bok Lee),양영헌(Yung Hun Yang) 한국생명과학회 2020 생명과학회지 Vol.30 No.11

본 연구는 지방구세포 감소를 위한 소재개발로 감국 추출물의 대표물질인 camphor가 갖는 지방구세포 분화억제효과를 관찰하였다. camphor의 세포독성을 확인하기 위해 3T3-L1 세포주를 이용하여 세포독성 측정을 1 day에서 10 day까지 확인하였고, 세포독성은 모두 없었다. PTCH siRNA를 처리 시 PTCH가 비활성되어 분화가 억제되었고, SMO siRNA 처리시 SMO가 비활성화되어 분화가 유도되었다. PTCH 억제는 SMO를 활성화시키는 기전으로HH 신호의 활성화가 지방구세포 분화를 억제시키는 것을 보여준다. FACS를 이용한 Gli1 발현은 KCTC 3237은 62.7±1.5%, camphor는 60.4±2.2% 로 분화된 세포 24.9±3.1% 보다 높은 것을 확인하였다. GC-MS에서 발효된 camphor의 구조변화는 없는 것으로 확인되었으나, KCTC 3237에서 15.41% 양적 증가를 확인했다. 또한camphor 가 SMO를 과발현시키고, Gli1의 변화를 조절하였다. 따라서 감국 발효물의 생리활성 물질 중 향후 매커니즘 분석을 위한 활성물질인 camphor를 이용해 동물모델에서 비만억제 효과에 대한 자료가 더 필요할 것으로 여겨지며, 감국 추출물 및 발효물의 HH신호조절이 새로운 비만치료제로 개발될 수 있는 가능성을 제시하고자 한다. In this study, we examined inhibition of adipocyte differentiation associated with the administration of camphor, a substance identified in extracts of the flowering plant Chrysanthemum indicum L. (CI). No camphor-mediated cytotoxicity was observed over a period of 1-10 days in studies targeting cells of the 3T3-L1 adipocyte-like line. Experiments that featured siRNA-mediated suppression of the transmembrane proteins Patched (PTCH) and Smoothened (SMO) resulted in inhibition and activation of differentiation, respectively. Interestingly, inhibition of PTCH typically activates SMO protein targeting and serves to activate hedgehog (HH)-mediated signaling. The results of our study suggest that activation of HH-mediated signaling can inhibit adipocyte differentiation. Furthermore, expression of glioma- associated oncogene homologue 1 (Gli1) was detected by flow cytometry in 62.7±1.5% of cells in response to administration of Lactobacillus rhamnosus (KCTC 3237) and in 60.4±2.2% of cells in response to camphor; these levels are higher than those detected in undifferentiated controls (24.9±3.1 %). No change in the state of fermented camphor was identified by gas chromatography-mass spectrometry (GC-MS), but a 15.41% quantitative increase was confirmed in KCTC 3237. Overall, we conclude that administration of camphor resulted in overexpression of SMO and modulated the differential expression of Gli1. Animal studies focused on the impact of camphor as an agent to counteract obesity might be considered in the future. Indeed, camphor and similar physiologically active compounds from fermented CI might be developed as new and effective treatments for obesity.