납(Pb)이 생쥐 대뇌피질내 몇 가지 효소황성 및 미세구조에 미치는 영향

이서은,유정규,최임순,Lee, Seo-Eun,Yoo, Chang-Kyu,Choe, Rim-Soon 한국현미경학회 1987 Applied microscopy Vol.17 No.2

This experiment was performed to investigate the acute and chronic effects of lead on cerebral cortex. In acute treatment, mouse were injected with lead acetate at dose of 0.3 mmole/kg body weight, and in chronic treatment, mouse were supplied 0.03 M lead acetate sol. in the place of water. After treatment, mouse were sacrificed at time intervals of 24, 48, 72, and 96 hours in acute treatment and at time intervals of 4 weeks and 8 weeks in chronic treatment. In acute treatment, acetylcholinesterase activity is reduced at 72 hours and recovered at 96 hours in homogenate, and reduced at 24 hours and recovered at 72 hours in crude synaptosomes. In chronic treatment, acetylcholinesterase activity is increased in young mouse but reduced in mother mouse. Ultrastructural changes were composed of swelling of Golgi apparatus, nerve terminals with diminished synaptic vesicles, and vacuolated myeline lamellae of myelinated axon.

솔잎 분말을 첨가한 스펀지 케이크의 품질 및 항산화 특성

이서은(Seo Eun Lee),이준호(Jun Ho Lee) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.1

솔잎 분말의 첨가농도를 0-8%로 달리하여 스펀지 케이크를 제조하고 물리화학적 품질, 항산화특성 및 소비자 기호도를 비교하였다. 솔잎 분말의 첨가량이 증가할수록 케이크의 pH는 단계별로 유의적으로 감소하였고, 반죽의 비중은 유의적으로 증가하였다(p<0.05). 한편 수분함량은 솔잎 분말 첨가에 따라 유의적으로 감소하였고 굽기손실은 증가하였다(p<0.05). 케이크의 비체적, 높이, 부피지수 및 대칭지수 또한 유의적으로 감소하였다(p<0.05). 밝기를 나타내는 L<SUP>*</SUP>값은 솔잎 분말 첨가량이 증가할수록 단계별로 유의적으로 감소하였으며, 적색도를 나타내는 a<SUP>*</SUP>값은 뚜렷한 증가를 나타내었다. 황색도를 나타내는 b<SUP>*</SUP>값은 솔잎 분말 첨가에 따라 급격히 감소하였으나 농도의 영향은 미미한 것으로 나타났다. 한편 케이크의 경도는 유의적으로 증가하였다(p<0.05). 항산화특성을 나타내는 총 페놀화합물 함량과 DPPH 전자공여능은 솔잎 분말 농도에 따라 증가하는 경향을 나타내었으며 그 증가 효과는 DPPH 전자공여능에서 뚜렷하게 나타났다. 소비자 기호도 검사 결과 대부분의 평가항목에서 대조군과 2%, 4% 첨가군 사이에 유의적인 차이가 없었으며, 따라서 스펀지 케이크의 관능적 품질과 건강 기능성 효과 등을 고려할 때 솔잎 분말을 2-4% 첨가한 스펀지 케이크가 가장 적절한 것으로 판단된다. The effect of baking on the physicochemical and sensory properties was investigated using a model system of sponge cakes incorporated with pine leaf powder (PLP) as a value-added food ingredient. PLP was incorporated into cake batter at 5 levels (0, 2, 4, 6, and 8%, w/w) by adding an equivalent amount based on the weight of wheat flour. After appropriate mixing, sponge cakes were baked at 170℃ for 30 min in an oven. The baked cakes were cooled to room temperature for 1 h prior to all measurements. The pH, moisture content, specific volume, height, volume index, and symmetry index decreased significantly with the increase in PLP content (p<0.05). On the contrary, specific gravity of the batter and baking loss increased (p<0.05). The lightness of the cakes decreased, whereas redness increased significantly with the higher amount of PLP in the formulation (p<0.05). Hardness also increased significantly (p<0.05). Both total polyphenol content and DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activityincreased significantly by the addition of PLP (p<0.05). The consumer acceptance test indicated that the addition of PLP up to 4% did not cause a significant unfavorable effect on the consumer acceptances in all attributes. The sponge cakes with 2-4% PLP would be recommended with respect to the overall acceptance score while taking advantage of the functional properties of PLP without sacrificing the consumer acceptability.

벌점회귀를 통한 상대오차 예측방법

정석오,이서은,신기일,Jeong, Seok-Oh,Lee, Seo-Eun,Shin, Key-Il 한국통계학회 2015 응용통계연구 Vol.28 No.6

본 논문에서는 상대오차의 개념과 벌점회귀를 결합한 새로운 예측방법을 제시하였다. 제안된 방법은 오차항의 분포가 정규성을 크게 벗어나 있어 이상점을 포함하거나 오차항의 분포가 심각하게 비대칭인 경우에도 안정적으로 예측력이 유지할 뿐 아니라 벌점회귀를 통한 변수선택의 성능도 우수하다. 또한 개념적으로 쉽고, 계산 속도가 빠르며, 기존의 알고리즘을 활용해 구현하는 것이 매우 쉽다. 한국교통연구원의 일일 차량통행량 자료 실제 분석 및 모의실험을 통해 제안된 방법의 우수한 성질을 확인하였다. This paper presents a new prediction method based on relative error incorporated with a penalized regression. The proposed method consists of fully data-driven procedures that is fast, simple, and easy to implement. An example of real data analysis and some simulation results were given to prove that the proposed approach works in practice.

전침자극이 흰쥐척수후각세포의 유해자극반응에 미치는 효과의 특성

신홍기,박동석,이서은,김진혁,Shin, Hong-kee,Park, Dong-suk,Lee, Seo-eun,Kim, Jin-hyuk 대한침구의학회 2002 대한침구의학회지 Vol.19 No.4

This experiment was designed to investigate the effects of electroacupuncture (EA) on chronic pains and factors that affected EA effects. The responses of wide dynamic range (WDR) cells to electrical stimulation of $A{\delta}$ & C afferent fibers were used as an index of pain in rats with chronic pains induced by intraplantar injection of complete Freund's adjuvant or peripheral nerve injury. In rats with chronic pains, low (2Hz) and high (100Hz) frequency EA stimulation applied to zusanli caused the inhibition of WDR cell responses in about 60% of rats and the inhibitory actions were dependent on the stimulus strength. EA stimulation also induced an excitation of WDR cell responses in 23.9% of rats and no effect in 15.8% of rats. However, it seemed that in normal rats compared to the rat with chronic pains, the incidence of which EA stimulation caused the excitation or no effect was high. Reversible spinalization almost completely blocked EA-induced inhibitory or excitatory effects. EA stimulation more frequently induced the excitation of WDR cell responses in lightly anesthetized (0.6%) rats and the enhanced responses of WDR cells were inhibited by EA stimulation in the rat anesthetized with 1.5% enflurane. These experimental findings suggest that in rats with chronic pain, EA stimulation inhibited WDR cell responses to slow $A{\delta}$ and C fiber stimulation and EA-induced inhibitory action was under the control of descending inhibitory system and degree of anesthesia.

만성통증을 억제하는 전침효과의 말초성 기전과 아편양물질수용기에 관한 연구

신홍기(Hong-Kee Shin),이서은(Seo-Eun Lee),박동석(Dong-Suk Park) 대한한의학회 2003 대한한의학회지 Vol.24 No.3

어린이, 청소년의 해부학과 임상 체험을 위한 안내서

정범선(Bum Sun Chung),고성배(Seong-bae Koh),박은미(Eun-mi Park),송미금(Mi-Geum Song),이서은(Seo-eun Lee),전태호(Tae-ho Jeon),조숙경(Sook-kyoung Cho),정민석(Min Suk Chung) 대한체질인류학회 2015 해부·생물인류학 (Anat Biol Anthropol) Vol.28 No.2

이 연구의 목적은 어린이, 청소년이 해부학과 임상을 체험하면서 의학, 나아가 생명과학에 관심을 갖게 만드는 것이다. 이를 위하여 컴퓨터로 사람 몸을 가상으로 체험할 수 있는 자료 (만화, 3차원영상, 2차원영상)와 임상 시술을 직접 체험할 수 있는 도구 (청진기, 혈압계, 손전등, 반사망치)를 어떻게 쓰는지 쉽게 풀이한 안내서를 만들었다. 과학관에 온 어린이, 청소년이 안내서에 따라서 해부학과 임상을 체험하였다. 즉 만화로 해부학을 익힌 다음에 각 구조물의 2차원영상, 3차원영상을 보았다. 이어서 임상 도구를 가지고 심장 소리를 듣고, 혈압을 재고, 동공반사와 무릎반사를 일으켰다. 이 연구를 통해서 어린이, 청소년이 해부학과 임상을 즐겁게 체험하는 것이 바람직하다는 것을 알았다. 이 안내서를 집, 과학관, 학교에서 더 잘 쓸 수 있도록 발전시킬 계획이다. The purpose of this study is to enable children and adolescents to experience anatomy and clinics. For the purpose, the ways to use the anatomy educational resources (comics, 3-dimensional images, and 2-dimensional images) and diagnostic tools (stethoscope, sphygmomanometer, pen light, and reflex hammer) were described in a guide book. Following the guide book, students experienced anatomy and clinics in a course of the science museum. They learned anatomy with the comics, then did virtual dissection with the 3-dimensional and 2-dimensional images. Sequentially, with the diagnostic tools, they listened to heart sound, measured blood pressure, and performed light reflex and knee jerk. Through this study, we have found that anatomy and clinics should be experienced pleasantly. The complimentary guide book is expected to be further improved in future, so as to achieve better experience at home, science museum, and school.

흰쥐 자궁에 유도된 염증으로 인한 substance P와 CGRP에 대한 면역반응의 변화

나중열(Joong Yol Na),장기훈(Ki Hoon Chang),유상욱(Sang Wook You),홍순철(Soon Choul Hong),이서은(Seo Eun Lee),한희철(Hee Chul Han) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5

N/A Objective : Recently some reports suggested substance P and CGRP might be important factors for inflammation and hyperalgesia. This study was performed to see whether substance P or CGRP containing nerve fibers might be changed by mustard oil-induced inflammation. Methods : After injection of mustard oil(5%) into uterine lumen, the uteri were removed and examined with immunohistochemical methods for substance P and CGRP. Results : In the normal uterus, most of the substance P- or CGRP-immunoreactive nerve fibers were observed along the vascular structure and some in the myometrium, only few in the endometrium. Mustard oil did not changed this pattern of nerve fiber distribution but after 48 hrs, the amount of substance P or CGRP immunoreactive nerve fibers were greatly reduced compared with the normal uterus. It is not clear whether the decrease of substance P and CGRP immunoreactive fibers in the uterus was resulted from the depletion of the neuropeptides in the nerve fibers or the retraction of nerve fibers. Conclusions : These results suggest that the inflammation should cause the change of nerve fibers included in the nociception. This change may attribute the generation of inflammation and inflammatory hyperalgesia.

Substance P에 의한 자궁의 기계적 수용체의 활동 변화에 대한 전기 생리학적 고찰

나중열(Joong Yol Na),장기훈(Ki Hoon Chang),신재철(Jae Chul Shin),유상욱(Sang Wook You),홍순철(Soon Choul Hong),이서은(Seo Eun Lee),한희철(Hee Chul Han) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.4

N/A Objective : In inflammation, hyperalgesia is a common phenomenon but its mechanism has not been clarified. Recently some reports suggested substance P might be important factors for inflammatory hyperalgesia in somatic tissue. This study was performed to see whether substance P modulate the activities of uterine afferent fibers in the hypogastric nerve of the cat. Methods : While recording the electrical activities of nerve fibers, mechanical stimuli were applied as balloon distention using balloon inserted into uterine lumen before and during substance P infusion through uterine artery. Results : Substance P increased the responses to balloon distension of uterus in 14 uterine mechanoreceptive afferent fibers of 24 over 10% compared to before substance P infusion, and decreased the responses of 3. And L-703,606, the neurokinin 1 receptor antagonist failed the modulation of mechanosensitive response by substance P and reduced the spontaneous activities. Conclusions : These results suggest that substance P modulated the activities of uterine nerve fibers and their responses to mechanical stimulus. It is hypothesized that this kind of modulation of afferent nerve fibers by substance P may be important for the development of inflammatory hyperalgesia.

고양이 방광의 기계적 수용체에 대한 노르에피네프린의 억제 효과

오현주,홍승길,민선식,이서은,황종문,강유진,한희철,윤영욱 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.40 No.3

Background: Recent findings suggest that a coupling between the somatic and sympathetic nervous system is critical not only for the development but also for the maintenance of pain behavioral changes. However, studies on the effect of sympathetic efferent system on sensory receptors in the visceral organ that is more dependent on the autonomic nervous system are lacking. This study examined whether norepinephrine (NE) had an influence on the mechanoreceptors in the feline urinary bladder. Methods: Ten adult male cats were used and anesthetized with α-chloralose and artificially ventilated. A cannula with the pressure transducer was inserted through the urethra to apply mechanical stimuli and monitor the pressure of bladder. A tiny caulula inserted into the bilateral side branches of vesical arteries were used as a route for a NE (10 ㎍ bilaterally) injection. Nerve fiber recordings were obtained from the distal stump of the pelvic nerve. Results: After the NE injection, the response of mechanoreceptors (n = 13) to the isotonic pressure stimulus (50-60 mmHg) decreased significantly (p < 0.05) in terms of sensitivity (i.e., ratio of nerve activity change to urinary bladder pressure change). The responses to pressure stimuli after an injection of an α 1 adrenoceptor blocker (terazosin) reversed the effect of NE. The responses of mechanoreceptors to isotonic pessure stimulus were not affected significantly by NE with preinjection of an α2 adrenoceptor blocker (yohimbine). Conclusions: These results suggest that NE may have influence on the sensitivity of mechanoreceptois in the normal feline urinary bladder via an α1 adrenoceptor. (Korean J Anesthesiol 2001; 40: 406~412)