대하(P. orientalis)로부터 분리정제한 Carotenoprotein의 특성에 관한 연구

이서구,김재웅,Lee, Seo Gu,Kim, Jae Ung 대한화학회 1994 대한화학회지 Vol.38 No.8

양식산 새우(P.orientalis)로부터 0.05M 인산염 완충용액(pH 7.0)으로 carotenoprotein을 추출하고 DEAE-셀룰로오스, 반포화 황산암모늄 친전 및 GPC로 $\alpha$-form과 ${\beta}$-form 두 종류의 carotenoprotein을 분리 정제하였다. 최대 흡수파장은 ${\lambda}_{max}$= 480, 409, 318 및 280 nm였다. SEM과 XRD로 분자구조를 관찰하였으며, GPC와 PAGE로 측정한 분자량은 $\alpha$-form이 170KDa으로 분리되는 불균일한 이합체 구조였다. carotenoprotein 복합체의 아미노산, 중성지질, 인지질 및 유리지방산 조성을 분석하였고, 여러 가지 유기화학 반응과 UV/Vis,IR, $^1H$-NMR, MS 등으로 carotenoids분자구조를 측정한 결과 lipid-protein의 보호그룹으로서는 astaxanthin과 그 ester임이 확인되었다. The isolation, purification and characterization of a carotenoprotein from the carapace of Pnaeus orientalis were investigated. The carotenoprotein was purple with broad λmax between 480, 409, 318 and 280 nm. Apparent structures were estimated by using X-ray diffractometry and scanning electron microscope, respectively. The molecular weight of the carotenoprotein complex had been determined by GPC and PAGE. The heavier complex, designated the $\alpha$-form (M.W = 170 KDa), was dissociated to a major subunit, $\beta$-form (M.W = 42 KDa). SDS-PAGE of $\alpha$-form showed apparently oligomeric pattern, and also $\beta$-form gave two polypeptides corresponding to 22 KDa and 19 KDa, respectively. The amino acid of the two proteins $({\alpha}-and\;{\beta})$-form, lipid and free fatty acid compositions were described. The prosthetic groups of the carotenoprotein were confirmed by TLC, IR, $^1H$-NMR, MS and various organic reactions as astaxanthin, astaxanthin monoester and astaxnathin diester.

시민천문대 현황과 역할 - 꿈과 희망을 여는 생활 속의 하늘 놀이터

이서구,Lee, Seo-Gu 한국과학기술단체총연합회 2010 과학과 기술 Vol.497 No.-

활성산소에도 순기능 있다

이서구,창동신,Lee, Seo-Gu,Chang, Dong-Sin 한국과학기술단체총연합회 2006 과학과 기술 Vol.39 No.9

변화되어 가는 어촌종합개발사업

이서구,Lee, Seo-Gu 한국어촌어항협회 2008 어항어장 Vol.82 No.-

어촌활성화를 위한 어촌정책 추진방향

이서구,Lee, Seo-Gu 한국어촌어항협회 2008 어항어장 Vol.83 No.-

병원동행자의 서비스 선택속성 : 광주광역시 광산구청 국민정책디자인 과제를 중심으로

이서구(Lee, Seo Gu),강동선(Kang, Dong Seon),이지영(Lee, Ji Young) 한국디자인리서치학회 2021 한국디자인리서치 Vol.6 No.4

본 연구의 목적은 광주광역시 광산구에서 국민정책디자인 과제로 추진하고 있는 건강약자에 대한 병원동행 서비스가 안정적으로 확산·정착할 수 있는 논거를 마련하는 것이다. 이를 위해 병원동행 서비스의 누수 및 사각지대를 최소화 될 수 있도록 서비스 대상인 건강약자에 대한 정의를 명확히 하였고, 국민정책디자인 과제 수행에서 다루지 않은 병원동행자의 서비스 선택속성에 대한 통계분석을 통해 서비스 필요성에 대한 객관적인 근거를 제시하였다. 연구결과 건강약자는 ‘신체적, 정신적으로 불편하여 지속적인 병원 진료가 필요하나 병원동행 없이는 이동이 불가능 한 사람’으로 정의하였고, 병원동행자의 서비스 수요도 다양한 집단에서 나타나고 있는 것을 확인하였다. 또한, 병원동행 서비스가 비즈니스 모델로 자리잡기 위한 제도적 개선, 병원동행 차량에 대한 지위 문제 명문화, 병원동행 서비스의 확장성에 대한 시사점을 도출하였다. The purpose of this study is to provide a rationale for stably spreading and establishing the hospital accompaniment service for the health vulnerable, which is being promoted as a national policy design project in Gwangsan-gu, Gwangju. In order to minimize leakage and blind spots of the hospital accompaniment service, the definition of the health vulnerable who are the target of the service was clarified, and objective grounds for service necessity were presented through statistical analysis on the service selection attributes of hospital companions, which were not addressed in the implementation of the national policy design task. As a result of the study, the infirm was defined as ‘a person who is physically and mentally uncomfortable and needs continuous hospital treatment, but is unable to move without a hospital companion’, and it was confirmed that the service demand of hospital companions also appeared in various groups. In addition, we drew implications for institutional improvement to establish the hospital companion service as a business model, stipulating the status issue for hospital companion vehicles, and scalability of hospital companion service.

관광객 특성에 따른 어촌체험프로그램 선택의 영향력 분석

이서구 ( Lee Seo-gu ),최규철 ( Choi Kyu-chul ),김정태 ( Kim Jung-tae ) 한국농촌계획학회 2020 농촌계획 Vol.26 No.3

The purpose of this study is to analysis the influence of fishing villages experience programs choice by the tourist characteristics. As an analysis method, a statistical technique of multinomial logistic regression was used. The dependent variable have typified about 70 fishing experience programs, such as tidal-flat experience, fishery experience, and fishing experience, operated by the fishing village experience recreation villages into 9 programs. The independent variables consisted of 7 groups of people: gender, age, marital status, presence of children, experience of visiting a village in a rural and fishing village experience, preference of a village in a recreational experience, and recognition of a village in a fishing village experience. As a result of analysis, no significant differences were found that the selection group preferring ‘fishing culture experience’, ‘leports experience’, ‘ecological craft experience’, and ‘festival and event experience’ in the selection of fishing village experience program compared to the group choosing ‘rural experience’. On the other hand, the group preferring ‘tidal flat experience’ analysis that ‘married’ is about 14 times higher than ‘unmarried’, and the group preferring ‘fishing village experience’ is 9.55 times higher than the group preferring ‘rural village experience’. In the group preferring ‘fishery experience’ and ‘fishing experience’, the group preferring ‘fishing experience recreation village’ was 9.21 times and 14.34 times higher than the group preferring ‘rural experience recreation village’. In the ‘food experience’, ‘married’ was 25 times higher than ‘unmarried’.

어촌 소멸위험의 영향 요인 분석

이서구 ( Seo-gu Lee ),김정태 ( Jung-tae Kim ) 한국수산경영학회 2020 수산경영론집 Vol.51 No.1

The purpose of this study is to propose a policy improvement plan by analysis of the extinction risk factors reflecting the specificity of fishing villages, fishing village support policies, and settlement conditions of fishing villages as one of the solutions to the immediate problem of fishing village extinction. The results of the study show the higher the level of number of fishing ports, number of returning rural population, and housing diffusion rate, the dependent variable extinction risk index was a positive effect while vacant house ratio and aged house ratio was analyzed to be in was a negative (-) relationship with the dependent variable.The policy implications through this study were to prepare an effective policy to reduce the risk of extinction, to improve urgent settlement conditions, and to prepare a condition to convert returning rural population into fishery population.

서비스 디자인 관점에서 어촌민박 비즈니스 모델에 관한 연구

이서구(Seo-Gu Lee),김정태(Jung-Tae Kim) 한국해양비즈니스학회 2021 해양비즈니스 Vol.- No.48

The purpose of this study is to present a business model in which the methodology from the perspective of service design, which is expanding into the public domain, is applied to fishing villages and to assess the possibility of its success. In order to present a business model for fishing villages, based on the results of field surveys, virtual personas were set as users and operators of fishing villages, and each customer journey map was analyzed. As a result, it was found that the common pain point felt in the process of each persona s experience was cleaning of the accommodation, especially hygienic bedding. Accordingly, based on the model for revitalization of fishing villages, which was suggested based on the sharing economy in previous studies, “shared washing” of fishing villages was discovered and presented as a business model.

어촌뉴딜300 사업의 경제적 파급효과 분석

이서구 ( Seo-gu Lee ),김정태 ( Jung-tae Kim ) 한국수산경영학회 2019 수산경영론집 Vol.50 No.3

The purpose of this study is to provide logical and policy justification for the feasibility and sustainability of the project through analysis of economic ripple effects of the fishing village new deal 300 project. To do this, we applied the industry-related analysis, which is mainly used to analyze the economic ripple effects, to the fishing village new deal 300 project. The industry association analysis classifies the detailed project of the preliminary plan for the selection of the business into the software business such as the hardware business and the capacity enhancement in the construction field and analyzes the economic ripple effect through the inter-industry association. As a result, it is expected that the fishing village new deal 300 project will have a positive economic impact. When the total investment of 3 trillion won is invested in the project, it is estimated that the production inducement effect and the value added effect are 5,545.3 billion won and 2,102.7 billion won, respectively. In addition, 62,005 get job inducements, where 10,952 employment inducements were associated with job creation. The analysis of the above impacts seems to have secured the logical justification for the implementation of the fishing village new deal 300 project.