Diisopropyl Fluorophosphate 생분해 효소의 유전자 재조합

이남택 陸軍士官學校 1994 한국군사학논집 Vol.46 No.-

DFPase gene originated from Flavobacteriun sp. was subcloned into expression vector, pRSET A. DFPase expressed from the gene can degradate diisopropylfluorophosphate(DFP) as well as parathion. The size of the DFPase gene is 1018 bp and it contains synthetic initiation codon with vector's termination codon. the recombinant expression vector will provide convenience to the purification of expressed protein due to histidine tag it contains.

02 21세기의 아킬레스건, 생물무기

이남택,Lee, Nam-Taek 한국화재보험협회 2013 防災와 保險 Vol.148 No.-

Hydrogenase from Desulfovibrio desulfricans: Its Purification and Characterization

이남택,양철학,Lee, Nam-Taek,Yang, Chul-Hak 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3

Cytochrome $C_3$의 존재하에 수소의 발생 및 흡수를 촉매하는 Hydrogenase를 Desulfovibrio desulfricans로부터 정제하였다. 세포벽에 붙어 있는 이 효소는 박테리아를 초음파로 분쇄한 후 Triton X와 sodium deoxycholate로 처리하여 $100,000{\times}g$로 초원심분리하고 다시 Sephadex G-150 및 DEAE-Cellulose 콜럼을 통과시킨 후 isoelectric focusing에 의하여 정제하였다. 분자량은 120,000으로 추정되었고 pH 적정점은 7.0 근처이었다. 이 효소는 Cytochrome $C_3$의 인위적인 전자전달 매개체인 환원된 methyl viologen으로부터 수소발생을 촉매하며, 이에 대한 Km 값은 0.2 M NaCl에서 $5.1{\times}10^{-5}M$이었고 NaCl의 농도가 증가함에 따라 그 값이 감소하였다. Isoelctric focusing에 의하여 측청된 pI 값은 6.1 근처이었으며 열(heat)에 대하여 상당히 안정한 것으로 나타났다. 그리고 이 효소는 중금속과 리간드를 형성하는 $NaN_3$, EDTA 및 KCN에 의하여 그 활동도가 억제되었으나 $Fe^{3+}$ 및 $Fe^{2+}$와 같은 금속이온에 의해서는 그 활동도가 현저히 촉진되었다. Hydrogenase which catalyzes the production and consumation of gaseous $H_2$ in the presence of cytochrome $C_3$ was purified from Desulfovibrio desulfricans. This particulate enzyme was solubilized in Tris-HCl buffer containing Triton X and sodium deoxycholate after sonication. This was further purified by ultracentrifugation ($100,000{\times}g$) of sonicate, gel filtration on Sephadex G-150, ion-exchange on DEAE-cellulose and isoelectric focusing. Its molecular wight was estimated to be 120,000 by gel filtration on Sephadex G-200 column chromatography and showed maximium activity around pH 7.0. This enzyme catalyzes the $H_2$ evolving reaction of methyl viologen which is an artificial electron carrier of ferricytochrome $C_3$. Its Km value was $5.1{\times}10^{-5}M$ but it was expected to show decrease according to the increase of salt concentration. pI value estimated by isoelectric focusing was 6.1 and it showed rather stable property upon heat inactivation. The enzyme was inhibited by metal complexing agents such as $NaN_3$, EDTA and KCN, but showed significant activation effect by metal ions such as $Fe^{3+}$ and $Fe^{2+}$.

Desulfovibrio desulfricans 로 부터 하이드로지나제의 분리 및 그 성질에 관한 연구

이남택,양철학 ( Nam Taek Lee,Chul Hak Yang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3

Hydrogenase which catalyzes the production and consumation of gaseous H₂ in the presence of cytochrome C₃ was purified from Desulfovibrio desulfricans. This particulate enzyme was solubilized in Tris-HCl buffer containing Triton X and sodium deoxycholate after sonication. This was further purified by ultracentrifugation (100,000×g) of sonicate, gel filtration on Sephadex G-150, ion-exchange on DEAE-cellulose and isoelectric focusing. Its molecular wight was estimated to be 120,000 by gel filtration on Sephadex G-200 column chromatography and showed maximium activity around pH 7.0. This enzyme catalyzes the H₂ evolving reaction of methyl viologen which is an artificial electron carrier of ferricytochrome C₃. Its Km value was 5.1×10^(-5) M but it was expected to show decrease according to the increase of salt concentration. pI value estimated by isoelectric focusing was 6.1 and it showed rather stable property upon heat inactivation. The enzyme was inhibited by metal complexing agents such as NaN₃, EDTA and KCN, but showed significant activation effect by metal ions such as Fe^(3+) and Fe^(2+).

제2장 육군의 미래 NCW 개념 및 구현 방안 연구

이남택,오명호,김태우,김영준,신내호 한국전략문제연구소 2006 한국전략문제연구소 전투발전 Vol.- No.-

전략물자 확산방지는 국방의 연장선

이남택 전략물자관리원 2011 전략물자 focus Vol.- No.4

생물무기의 국제적 위협 및 미국의 대비실태 분석

이남택 원광대학교 2005 圓光軍事論壇 Vol.- No.1

Due to the excessive concerns about nuclear and chemical weapons and ballistic missile proliferation, biological weapons and biological arms control were overshadowed during the Cold War. Taking advantage of the loose control by international treaties, such as Biological Weapons Convention (BWC), and also due to the wide availability of key biological-related technologies and know-how, poor countries tried to develop and possess biological weapons. These countries knew that lethal efficiency of biological weapons was even more potential than that of nuclear weapons, and that they could not win out over advanced countries like United States in a conventional war. All of these aspects were enough to stimulate less developed countries to seek for unconventional or asymmetrical weapons. As a result of such pursuit, many countries became to possess biological weapons and are showing a potential threat to the global society. By the way, North Korea, a hostile country to us, has already equipped with biological weapons as well as chemical and unclear weapons. This situation is pressing Korean government to prepare with countermeasures against them. The purpose of this article is to analyze the international threat posed by biological weapons and its countermeasures by the United States government. A careful study of the United States' efforts on this issue would be beneficial to the preparation of Korean governmental countermeasures against the threat of North Korea's biological weapons.

Desulfovibrio Desulfuricans로 부터 하이드로지나제를 정제하는데 있어서 HPLC시스템과 IEFCC시스템의 비교

李南澤 陸軍士官學校 1985 한국군사학논집 Vol.28 No.-

HPLC System을 이용하여 수소의 발생 및 흡수를 촉매하는 하이드로지나제를 Desulfovibrio desulfuricans로부터 신속히 분리하였다. 이 HPLC System을 통하여 얻은 5가지 종류의 단백질 분포도는 IEFCC System을 통하여 얻은 단백질 분포도와 정확히 일치하여 HPLC System을 통하여서도 하이드로지나제의 정제가 가능함을 보였으며, 더구나 이 system을 통하여 얻은 활성 하이드로지나제를 수직 Slap gel 영동장치를 이용하여 분석한 결과 단일 띠가 나타났는데 이로보아 HPLC System을 통하여 얻은 하이드로지나제의 순도가 매우 높음을 알 수 있다.

발암성 단백질인 타이로신 키나제 유전자의 재조합 및 발현

이남택,백영진 陸軍士官學校 1992 한국군사학논집 Vol.42 No.-

TPK plays a central role in the signal transmission of the receptors and the regulation of cellular growth and differentiation. This protein can transform the normal cells into neoplasmic cells. It will therefore be important to compare the TPKs from both normal cells and neoplasmic cells to illucidate the transforming activity. It was, however, almost impossible to purify enough amount of TPK to study at the molecular level. To solve this problem, we tried to purify TPK using genetic engineering method. For this pGEX-ABL was prepared by fusing 1.2kb b-abl gene in front of glutathione S-transferase gene in the pGEX-2T vector and was expressed in the bacteria. The 1.2kb b-abl region has been defined to have minimal region required for both TPK activity and transformation of cells. The molecular weight of the fusion protein expressed from pGEX-ABL was 72.5kDa, whereas the v-abl gene product itself was determined to have the molecular weight of 45kDa.

Threats Posed by Chem-Bio Terrorisms and Countermeasures thereof

이남택 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

In terms of public health and national security, typical substances that pose threats to our daily lives are toxic compounds and pathogens. Among these threats, the first one is from toxic chemical weapons. Chemical weapons are not only used as asymmetrical weapons, but also as terrorist tools. The second one comes from various diseases, which in turn can be classified into three forms depending on the source of the pathogens: natural disease (MERS, Covid-19), intentional release (2001 Anthrax terrorism), and accidental release (Sverdlovsk Anthrax release). This presentation is to address the status of threats posed by chemical and biological agents, the current status of ROK and USA preparedness against biothreats, and gaps to be improved, thereby contributing to the preparedness of the ROK government in these fields.