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토양전염성 맥류바이러스 발생지역 및 맥류품종의 저항성 조사
이귀재,김형무,이왕휴 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.3
Soil-borne barley viruses were investigated in 42 regions during 1999-2003, in Korea. BaYMV, BaMMV and SbWMV symptoms were found in 37 regions. Crops resistance to mosaic viruses were investigated by growing them on 10 regions infected with soil-borne viruses. It was found that 10 unhulled barley, 6-beer bar]ey, 16 rye and 2 wheat species showed resistance to virus. Most of 15 Japanese cultivars showed infection symptoms but many of them showed relatively higher resistance in Ikasan, Youngkwang and Yesan areas region. Mixed virus infection was investigated and it was found that inter-regional species except in 10 areas, were mix infected with BaYMV and BaMMV. But in Youngkwang area all of crop species except Secheon-6 were infected only with BaYMV. Japanese cultivars were mixed infected with BaMMV and BaYMV except at Yesan that were infected only with BaYMV. 1999년부터 2001년까지 전국42개 지역에서 토양전염성 맥류바이러스를 조사한 결과 강원도 영월, 전라북도 무주, 충청남도 공주, 보령, 서천 포장을 제외한 37개 포장에서 BaYMV, BaMMV 및 SbWMV가 발생하고 있었다. 또한 토양전염성 맥류바이러스 상습 발병지 10개 장소에 겉보리 10품종, 쌀보리 16품종, 맥주보리 6품종, 밀 2품종을 파종하고 저항성 조사를 실시한 결과 겉보리는 조강보리, 쌀보리는 내한쌀보리 및 논산과1-6품종이 비교적 여러지역에서 저항성 높은 품종으로 나타났다. 그리고 일본에서 분양 받은 15품종도 대부분이병성으로 나타났으나 특이하게 익산, 영광 및 예산지역에서 저항성 품종이 많았다. 지역간 품종에 따른 바이러스 혼합 감염을 조사한 결과 조사 10개 지역 모두 BaYMV, 및 BaMMV가 혼합 감염되어 있었고, BbWMV는 예산, 진주, 나주 및 익산지역에 혼합감염되어 있었다. 그러나 영광지역에서는 사천6호 품종을 제외하곤 BaYMV만이 발생하였다. 일본 분양 품종은 예산에서만 BaYMV가 감염되었고 나머지 지역에서 모두 BaMMV 및 BaYMV에 혼합 감염되 어 있었다.
RP-PCR을 이용한 보리누른모자이크바이러스 (BaYMV)와 보리마일드모자이크바이러스(BaMMV)의 외피단백질 유전자 검정 및 해석
이귀재 한국식물병리학회 1998 Plant Pathology Journal Vol.14 No.4
Using the reverse transcription polymerase chain reaction (RT-PCR), a rapid and sensitive assay method for the detection and identification of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) was adapted. Two units of primers from each virus were selected and used for the determination of two different viruses. PCR fragments of BaYMV (ca. 0.9kb) and BaMMV (ca. 0.8kb) were obtained from the designed method for the assay of BaYMV and BaMMV coat protein. PT-PCR fragments were cloned using vector pT7 Blue and the sequences of the selected clones were analyzed. coat protein of BaYMV and that of BaMMV consisted of 297 amino acids (891 nucleotides) and 251 amino acids (753 nucleotides), respectively. The snalysis of coat protein genes from these two viruses showed that 45.6% of nucleotides sequence ad 34.9% of amino acid in BaYMV were homologous to those in BaMMV.
감나무 가지마름病에서 分離한 Pestalotia kaki의 2 系統
李貴宰,田吉亨,金炯武,蘇仁永,崔定植 전북대학교 농업과학기술연구소 1994 農大論文集 Vol.25 No.1
A black spot and die-back symptoms were severely occurred on the branch of young persimmon tree at Chonju and Haenam between 1991 and 1992. The Pestalotia kaki isolated from symptoms were investigated in cultural character and pathogenicity. Pestalotia kaki was isolated from the black spot and die-back symptoms. Pestalotia kaki was classified into the strain I and Ⅱ by cultural character. The color of mycelium on the PDA, the strain l was white and turned yellowish in the center, and the strain Ⅱ was white. Conidia formed concentric Bones in the strain Ⅱ, and scattered in the strain Ⅰ . Pathogenicity of the strain I and Ⅱ was simillar.
효소촉매 가교반응을 이용한 스프레이형 젤라틴 하이드로젤 창상피복제의 개발
김귀재(Gwijae Kim),고영광(Young-Gwang Ko),이혜영(Hyeyoung Lee),정호윤(Ho Yun Chung),권오형(Oh Hyeong Kwon) 한국고분자학회 2021 폴리머 Vol.45 No.4
생체적합성, 창상치유능 및 피부밀착성을 가지는 동시에 광범위하고 굴곡진 창상부에 간편하고 위생적으로 적용할 수 있는 스프레이형 창상피복제를 개발하고자 효소촉매 가교반응을 이용하여 티라민으로 개질된 젤라틴(Gel-Tyr) 하이드로젤을 제조하였다. Gel-Tyr의 개질률은 ¹H NMR을 통해 산출하였고 젤라틴, horseradish peroxidase(HRP) 및 H₂O₂의 농도에 따른 하이드로젤의 젤화시간, 유변학적 특성, 팽윤도, 생분해도, in vitro 세포독성, 세포생존거동을 평가하였다. 신속한 젤화속도와 창상피복제로서의 최적성능 조건의 하이드로젤은 배합농도 Gel-Tyr 3 wt%, HRP 3.50 units/mL 및 H₂O₂ 0.2 μL/mL로 제조된 시료로 선정하였다. In vivo 전층창상모델 동물실험 및 조직학적 평가에서 Gel-Tyr 하이드로젤은 대조군보다 촉진된 창상회복속도와 진피재생능을 보여주었으며, 의료현장에서 편리하게 사용할 수 있는 스프레이형 창상피복제로의 활용이 기대된다. The gelatin hydrogel modified with tyramine (Gel-Tyr) was prepared an enzymatic crosslinking reaction to develop a sprayable wound dressing that is easy to use and hygienic on broad and complicate wound area with biocompatibility, wound healing ability, and comfortable wearing. The substitution rate of Gel-Tyr was calculated through ¹H NMR. And the gelation time according to the concentration of gelatin, horseradish peroxidase (HRP) and H₂O₂, rheological properties, swelling ratio, biodegradation behavior, in vitro cytotoxicity, cell viability was evaluated. The hydrogel with rapid gelation rate and optimum performance as a wound dressing was selected as a sample prepared with a compound concentration of Gel-Tyr 3 wt%, HRP 3.50 units/mL, and H₂O₂ 0.2 μL/mL. In the in vivo full-thickness wound model animal experiment and histological evaluation, the Gel-Tyr hydrogel showed an accelerated wound healing rate and dermal regeneration compared to the control group. It is expected to be used as a sprayable wound dressing in medical treatment, conveniently.
수술환자의 혈압 변동에 관한 임상적 관찰 : 마취회복실을 중심으로
김귀재 최신의학사 1974 最新醫學 Vol.17 No.7
To observe the change of blood pressures in the surgical' patients, clinical' records-of 233-surgical' patients who were operated under the general anesthesia at the Kyung Pook National'. University Hospital during recent 3 years, have been analyzed. Among 233 patients-, 100 patients were major operation group, 85 patients were medium operation group and 48 patients were minor operation group depending upon the length of operating time and the degree of operative` trauma: The results was summarized as followings; 1. The preoperative: blood pressures seemed to, be increased than the controls blood; pressures; measured at the ward previously. 2. The postoperative blood pressures measured in the recovery room seemed to be decreased than the control blood pressures. 3. It is our. observation that the postoperative blood pressures in the recovery room were much decreased than minor operation owing to the degree of the surgical trauma and the length of the operating time.