학부모 입장에서의 공감과 소통

고영광 열린부모교육학회 2023 열린부모교육학회 정기학술대회 Vol.28 No.-

Growth Behavior of Endothelial Cells According to Electrospun poly(D,L-Lactic-Co-Glycolic Acid) Fiber Diameter as a Tissue Engineering Scaffold

고영광,박주희,이재백,오환희,박원호,조동환,권오형 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.4

Investigating the effect of electrospun fiber diameter on endothelial cell proliferation provides an important guidance for the design of a fabric scaffold. In this study, we prepared biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) fibrous nonwoven mats with different fiber diameters ranged from 200 nm to 5 μm using the electrospinning technique. To control the fiber diameters of PLGA mats, 4 mixture solvents [hexafluoro-2-propanol, 2,2,2,-trifluoroethanol:dimethylformamide (9:1), 2,2,2,-trifluoroethanol:hexafluoro-2-propanol (9:1), chloroform] were used. Average diameters were 200 nm, 600 nm, 1.5 μm, and 5.0 μm, respectively. Stereoscopic structure and spatial characterization of fibrous PLGA mats were analyzed using atomic force microscopy and a porosimeter. The mechanical properties of PLGA mats were analyzed using a universal testing machine. The spreading behavior and infiltration of endothelial cells on PLGA mats were visualized by field emission scanning electron microscopy and hematoxylin and eosin staining. Cell proliferation on different PLGA fibers with different diameters was quantified using the MTT assay. Cells on 200 nm diameter PLGA mats showed rapid attachment and spreading. However, the cells did not penetrate the PLGA mat. Cells cultured on 600 nm and 1.5 μm diameter fibers could infiltrate the pores and cell proliferation was dramatically increased after 14 days. Secreted prostacyclin from endothelial cells on each mat was measured to examine the ability to inhibit platelet activation. This basic study on cell proliferation and fiber diameter with physical characterization provides a foundation for studies examining nonwoven fibrous PLGA mats as a tissue engineering scaffold.

폴리감마글루탐산을 함유하는 생분해성 나노섬유의 제조 및 생의학적 응용

고영광,김원일,김철주,권오경,황진상,권오형,Ko, Young-Gwang,Kim, Won-Il,Kim, Cheol-Joo,Kwon, Oh-Kyoung,Hwang, Jin-Sang,Kwon, Oh-Hyeong 한국섬유공학회 2010 한국섬유공학회지 Vol.47 No.2

One of the serious complications that have not been solved yet in modern medicine is a postoperative tissue adhesion of internal organs. Tissue adhesions are associated with numerous postoperative complications, including pain, functional obstruction and difficult re-operative surgery. In this study, poly($\gamma$-glutamic acid)($\gamma$-PGA) based nanofiber sheets were prepared by using electrospinning technique to evaluate the ability of the prevention of postoperative tissue adhesion. The anti-adhesion membranes were prepared from $\gamma$-PGA and poly(D,L-lactic-co-glycolic acid)(PLGA) with different compositions by electrospinning. Also non-steroidal anti-inflammatory drug (ibuprofen) was incorporated during fabrication of nanofibers. Various electrospun nanofibers were characterized by SEM, FTIR, water contact angle measurement, biodegradability test, in vitro drug release profile, cell culture test, and in vivo animal study using Sprague Dawley rat model. The average diameter of the nanofibers electrospun from the various biodegradable polymer solutions ranged from 300 nm to 900 nm, approximately. From in vivo animal study, it was observed that ibuprofen-incorporated $\gamma$-PGA nanofiber sheet (crosslinked) was significantly effective in preventing post-surgical tissue adhesion and wound healing, probably due to the appropriate hydrophilicity preventing shrinkage of the sheet. On the other hand, PLGA nanofibrous mat was dramatically contracted in vivo due to its high hydrophobicity and resulted in insufficient coverage of wound.

은 나노 입자를 함유한 생분해성 나노섬유의 제조 및 특성분석

고영광,윤관한,강인규,권오형,김영진,김현숙 한국생체재료학회 2006 생체재료학회지 Vol.10 No.1

Silver ions have been widely used in various biomedical fields such as wound dressing materials, body wall repairs, augmentation devices, tissue engineering scaffolds. In this study, we prepared biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous mats containing silver nanoparticles by electrospinning technique and determined their antimicrobial activity. The average diameter of electrospun PHBV nanofibers decreased with the decrease in solution concentration and increase in electric potential and tip-to-collector distance. Electrospun structures revealed randomly aligned fibers with an average diameter of 380 nm. It could be controlled the electrospun fiber diameter by adjustment of electrospinning parameters, including solvent properties, concentration, applied voltage, solvent volatility and tip-to-collector distance. From the antimicrobial activity test, electrospun PHBV nanofibers containing 5000 ppm of silver nanoparticles showed more than 99.9% of bactericidal activity against Staphylococcus aureus and Escherichia coli. From the above results, electrospun biodegradable PHBV nanofibrous mat containing silver nanoparticles proved as an attractive antimicrobial materials. We believe that the electrospun nanofibrous mat containing silver nanoparticles holds great promise for various biomedical applications.

아버지-유아를 위한 신체활동 프로그램 개발 및 효과검증

고영광,송승민 한국아동가족복지학회 2022 한국가족복지학 Vol.27 No.4

Objective: This study aims to develop a father-child physical activity program and to investigate the effectiveness of the program for improving preschoolers' self-regulation, fathers' playfulness, and parenting efficacy. Methods: The development of the father-child physical activity program was as follows. First, the purpose of the program was to improve the ability of preschoolers' self-regulation, fathers’ playfulness, and parenting efficacy. Second, the contents of the program consisted of physical activities between the father and child using household goods and recycle materials. Third, on-line communication between childcare centers and families were carried out through the education of non-face-to-face methods. The father filmed and shared videos of the father and child playing at home. Results: First, the father- child physical activity program positively affected preschoolers' self-regulation abilities. Second, the program showed a positive effect on father's playfulness. Third, the program had a positive effect on the father's parenting efficacy. Conclusions: The father-child physical activity program is effective in improving preschoolers' self-regulation, fathers' playfulness, and parenting skills

Guiding Bone Regeneration Using Hydrophobized Silk Fibroin Nanofiber Membranes

고영광,이민영,박원호,조동환,권오경,권오형 한국고분자학회 2016 Macromolecular Research Vol.24 No.9

Biocompatible barrier membranes with both hydrophobic and hydrophilic surface properties provide critical backup for guided regeneration at localized bone defects without soft tissue invasion. As a surface modified functional barrier, saturated fluorocarbon (CF4)-immobilized nanofibrous silk fibroin (SF) membranes were fabricated by electrospinning for a fibrous non-woven mat, water vapor treatment for insolubilization, and followed by CF4 gas plasma treatment for top surface hydrophobization. Plasma-treated SF nanofiber membranes maintained a non-woven mat structure without shrinkage and deformation in a five-month biodegradation test. From in vivo rabbit cranium perforation model, nanofibrous SF membranes prevented soft tissue invasion and facilitated volumetric bone regeneration compared with the control groups. New bone ingrowth in bone defects at 4 and 8 weeks after surgery was visualized by trichrome staining. Medical application of fluorocarbon-immobilized nanofibrous SF barrier membranes could be one of the practical approaches for guided bone regeneration.

플리감마글루탐산을 주성분으로 하는 조직유착방지제의 제조

고영광,박청,선문희,훤오형 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.4

Modification and optimization of electrospun gelatin sheets by electron beam irradiation for soft tissue engineering

이재백,고영광,조동환,박원호,권오형 한국생체재료학회 2017 생체재료학회지 Vol.21 No.3

Background: Crosslinked gelatin nanofibers are one of the widely used scaffolds for soft tissue engineering. However, modifying the biodegradation rate of chemically crosslinked gelatin is necessary to facilitate cell migration and tissue regeneration. Here, we investigated the optimal electron beam (e-beam) irradiation doses with biodegradation behavior on changes in the molecular weight, morphology, pore structure, and cell proliferation profiles of electrospun nanofibrous gelatin sheets. Methods: The molecular weights of uncrosslinked gelatin nanofibers were measured using gel permeation chromatography. The morphology and pore structure of the gelatin scaffolds were analyzed by scanning electron microscopy and a porosimeter. Biodegradation tests were performed in phosphate-buffered saline solutions for 4 weeks. Cell proliferation and tissue regeneration profiles were examined in fibroblasts using WST-1 assays and hematoxylin and eosin staining. Results: Crosslinked gelatin nanofiber sheets exposed to e-beam irradiation over 300 kGy showed approximately 50% weight loss in 2 weeks. Gelatin scaffolds exposed to e-beam irradiation at 100–200 kGy showed significantly increased cell proliferation after 7 days of incubation. Conclusions: These findings suggested that the biodegradation and cell proliferation rates of gelatin nanofiber scaffolds could be optimized by varying e-beam irradiation doses for soft tissue engineering.

혈액응고인자를 함유하는 생체흡수성 폴리비닐피롤리돈 나노섬유시트 지혈제의 개발

심은정,고영광,권오경,김은진,권오형 한국고분자학회 2019 폴리머 Vol.43 No.4

본 연구에서는 생체친화적이고 보관 및 사용이 편리한 동시에 우수한 지혈능을 가지는 지혈제를 개발하고자염화칼슘과 트롬빈이 함유된 폴리비닐피롤리돈(PVP) 나노섬유형 지혈제를 제조하였다. 나노섬유의 미세구조, 원소함량비, 혈액응고인자의 분산도는 전자현미경과 에너지분산형 분광분석법을 통해 확인하였다. PVP 나노섬유에 포함된 트롬빈 및 염화칼슘의 비율에 따른 in vitro 전혈응고실험에서 5.5 mg/mL 염화칼슘 및 500 units/mL 트롬빈을함유한 샘플의 지혈능이 가장 높게 나타났으며, 세포생존율 90% 이상의 낮은 세포독성을 나타내었다. In vivo Sprague-Dawley(SD) rat 간출혈 모델 실험에서도 나노섬유 지혈제는 대조군보다 감소된 출혈시간과 출혈량을 보여주었다. 염화칼슘과 트롬빈이 함유된 PVP 나노섬유시트는 고성능 지혈제로 의료현장에서의 활용이 기대된다.

어머니의 사회성 발달에 관한 신념이 유아의 또래유능성에 미치는 영향: 또래관계 관리전략의 매개효과를 중심으로

송애란,고영광,엄희경,송승민 한국보육지원학회 2019 한국보육지원학회지 Vol.15 No.6

Objective: This study examined the relationship between maternal beliefs on social development, management strategies of peer relations, and their child’s peer competence in order to understand how to promote children’s peer competence. Methods: Self-report questionnaires were used on 195 mothers with children four to years old. Data were analyzed using Pearson Correlation Analysis and Baron and Kenny’s three-step regression. Results: First, there was a positive relationship between maternal beliefs on social development and their child’s peer competence. Second, this study found that there were partial mediating effects of advice·support and supervision on the relationship between maternal beliefs on social development and children’s social competence. Conclusion/Implications: The findings confirm that mothers’ beliefs on social development and management strategies of peer relationships are proceeding factors for children’s peer competence. Significant attention should be paid to mothers’ beliefs on social development and management strategies of peer relationships for children’s positive social development.