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L-Asparaginase가 Mouse의 肝細胞에 미치는 影響
鄭鎬三,李圭植,尹康默 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1
L-asparaginase hydrolysis L-asparagine which is essential amino acid for tumor cells to produce an antitumorous effect. On the other hand, it was reported that this drug provoked cytotoxic effect following the inhibition of protein synthesis and that this was a consequence of the exhaustion of L-asparagine in the normal cell. Therefore, the authors have demonstrated the toxicity of L-asparaginase on the hepatic parenchymal cell observing with light and electron microscope: Male mice of ICR strain were used in this experiment and the weight of experimental animals was around 20gm. The animals were divided into two groups, the control and the L-asparaginase treated groups and subdivided into the 6th, 12th and 24th hours groups. L-asparaginase 2,500 I.U./kg, in the water for injection was injected intraperitoneally for the animals of L-asparaginase treatment group. The animals of control group were given water for injection only. The experimental animals were sacrificed at the 6,12 and 24 hours after an administration of L-asparaginase or water for injection. The liver specimens were stained with hematoxylin oesin, methyl green-pyronin and oil red 0 method and observed them under the light microscope. And the ultrathin sectioned specimens were stained with uranyl acetate and lead citrate and observed with the JEM 100B electron microscope. The results obtained were as follows: 1. Cellular swelling accompanied with the vesicular or vacuolar formation, reduction or disappearance of the pyroninophilic granules and fat infiltration were observed in the hepatic parenchymal cells of the L-asparaginase treated mouse. 2. Dilatation, sacculation and fragmentation of the cisternae of RER were accompanied with dissociation of membrane bound ribosomes, and proliferation of SER associated with depletion of glycogen particles were observed. And formation of numerous primary lysosomes, secondary lysosomes, autophagic vacuoles, multivesicular bodies and irregular sized lipid droplets were recognized in the hepatic parenchymal cells of the L-asparaginase treated mouse. Consequently, it is suggested that intraperitoreal injection of L-asparaginase would include the cellular degeneration of the hepatic parenchymal cells in the mouse.
고양이에서 유발된 장액성중이염시 중이점막의 미세구조에 관한 연구
박순일,윤강묵,심상열,Park, Soon-Il,Yoon, Kang-Mook,Shim, Sang-Yul 한국현미경학회 1982 Applied microscopy Vol.12 No.1
Serous otitis media is closely related with auditory tube function, but its etiology and pathogenesis are not clearly defined yet. So we tried to prove the theory of hydrops ex vacuo via the experimental study with cats by means of obstructing the pharyngeal orfice of the auditory tube and observe the serial changes in tympanic mucosa through light and electron microscopy. The results are as follows; 1. We confirmed the production of serous otitis media with auditory tube obstruction and have a new understanding of auditory tube function in middle ear aeration. 2. The effusion in serous otitis media was produced from the next day of experiment and increased till the fourteenth day, but decreased after the spontaneous perforation of ear drum. 3. Through the light microscopy, we observed the increasement of the sercretory cells including goblet cells, epithelial hyperplasia, capillary proliferation and invasion of inflammatory cells. 4. Through the elctron microscopy, we observed the protrusion of secretory cells, blobs in cilia, loss of cilia, increasement of vesicles, vacuoles and dense bodies in ciliated cells and Invasion of inflammatory cells. With above results, we concluded that aeration through auditory tube is the most important factor in serous otitis media and presumed the effusion was secreted by secretory cells.