근권미생물에 의한 식물의 생물·환경적 복합 스트레스 내성 유도

유성제,상미경,Yoo, Sung-Je,Sang, Mee Kyung 한국식물병리학회 2017 식물병연구 Vol.23 No.2

식물은 재배기간 동안 세균, 진균, 바이러스 등의 생물 스트레스뿐만 아니라 고온, 염, 건조 등 다양한 환경 스트레스에도 노출되어 왔다. 최근에는 기후 이상현상으로 인하여 환경 스트레스의 빈도 및 강도가 불규칙적으로 증가하고 있으며 이로 인해 병원균의 생장과 영향도 변화하여 생물과 환경의 복합 스트레스가 식물 재배에 큰 영향을 주고 있다. 유용미생물을 이용한 식물의 저항성 유도는 다양한 생물과 환경 스트레스로부터 식물을 보호하는 데 도움을 주며, 이러한 스트레스에 대한 피해를 감소시킬 수 있는 가능성을 열어 주었다. 본 리뷰에서는 식물의 생물과 환경 스트레스에 대한 피해를 감소시키는 데 영향을 주는 미생물의 결정인자에 대해 기술하였으며 미생물 결정인자에 의해 유도되는 식물 신호전달 체계 변화에 대해 기술하였다. 또한 복합 스트레스 경감을 위한 미생물의 역할과 연구 방향에 대해 기술하였다. 이 리뷰를 통해 변화하는 환경에 대비하기 위해서 다양한 방안을 마련하고 있는 농민들에게 도움이 되기를 바라며, 실제 유용미생물 연구가 식물 재배 중 발생할 수 있는 다양한 스트레스에 따른 농가 피해를 감소시킬 효과적 대응 방안으로 이어지길 바란다. Recently, global warming and drastic climate change are the greatest threat to the world. The climate change can affect plant productivity by reducing plant adaptation to diverse environments including frequent high temperature; worsen drought condition and increased pathogen transmission and infection. Plants have to survive in this condition with a variety of biotic (pathogen/pest attack) and abiotic stress (salt, high/low temperature, drought). Plants can interact with beneficial microbes including plant growth-promoting rhizobacteria, which help plant mitigate biotic and abiotic stress. This overview presents that rhizobacteria plays an important role in induced systemic resistance (ISR) to biotic stress or induced systemic tolerance (IST) to abiotic stress condition; bacterial determinants related to ISR and/or IST. In addition, we describe effects of rhizobacteria on defense/tolerance related signal pathway in plants. We also review recent information including plant resistance or tolerance against multiple stresses ($biotic{\times}abiotic$). We desire that this review contribute to expand understanding and knowledge on the microbial application in a constantly varying agroecosystem, and suggest beneficial microbes as one of alternative environment-friendly application to alleviate multiple stresses.

토마토에 염류 내성을 유도하는 바실러스 균주 처리 후 근권 미생물 군집 구조 연구

유성제,이신애,원항연,송재경,상미경,Yoo, Sung-Je,Lee, Shin Ae,Weon, Hang-Yeon,Song, Jaekyeong,Sang, Mee Kyung 한국환경농학회 2021 한국환경농학회지 Vol.40 No.1

BACKGROUND: Soil salinity causes reduction of crop productivity. Rhizosphere microbes have metabolic capabilities and ability to adaptation of plants to biotic and abiotic stresses. Plant growth-promoting bacteria (PGPB) could play a role as elicitors for inducing tolerance to stresses in plants by affecting resident microorganism in soil. This study was conducted to demonstrate the effect of selected strains on rhizosphere microbial community under salinity stress. METHODS AND RESULTS: The experiments were conducted in tomato plants in pots containing field soil. Bacterial suspension was inoculated into three-week-old tomato plants, one week after inoculation, and -1,000 kPa-balanced salinity stress was imposed. The physiological and biochemical attributes of plant under salt stress were monitored by evaluating pigment, malondialdehyde (MDA), proline, soil pH, electrical conductivity (EC) and ion concentrations. To demonstrate the effect of selected Bacillus strains on rhizosphere microbial community, soil microbial diversity and abundance were evaluated with Illumina MiSeq sequencing, and primer sets of 341F/805R and ITS3/ITS4 were used for bacterial and fungal communities, respectively. As a result, when the bacterial strains were inoculated and then salinity stress was imposed, the inoculation decreases the stress susceptibility including reduction in lipid peroxidation, enhanced pigmentation and proline accumulation which subsequently resulted in better plant growth. However, bacterial inoculations did not affect diversity (observed OTUs, ACE, Chao1 and Shannon) and structure (principle coordinate analysis) of microbial communities under salinity stress. Furthermore, relative abundance in microbial communities had no significant difference between bacterial treated- and untreated-soils under salinity stress. CONCLUSION: Inoculation of Bacillus strains could affect plant responses and soil pH of tomato plants under salinity stress, whereas microbial diversity and abundance had no significant difference by the bacterial treatments. These findings demonstrated that Bacillus strains could alleviate plant's salinity damages by regulating pigments, proline, and MDA contents without significant changes of microbial community in tomato plants, and can be used as effective biostimulators against salinity stress for sustainable agriculture.

토마토 염류와 온도 스트레스에 대한 내성을 유도하는 미생물 선발

유성제,신다정,원항연,송재경,상미경 한국유기농업학회 2018 韓國有機農業學會誌 Vol.26 No.3

국내 일부 시설재배지는 장기간 과도한 양분 투입 등에 의한 염류 집적 현상이 문제가 되어왔으며, 최근 이상기온에 따른 온도장해에 의한 피해도 발생하고 있다. 이러한 현상에 대해 친환경적으로 대처하기 위하여 고염류와 온도 스트레스에 대해 작물에 내성을 증강시키는 미생물을 선발하였다. 국내 토양에서 분리한 1,944균주중 고염류 또는 온도 스트레스 조건에서 세균의 생장과 식물생장촉진 관련 특성(IAA 생성, ACC deaminase 활성, 인산가용화능)을 고려하여 20균주를 1차 선발(전체 균주의 1.03%)하였다. 1차 선발한 20균주 중 토마토 식물검정을 통해 고염류 또는 온도스트레스에 대한 내성을 유도하는 7세균(1차 선발균주의 35%, 전체 균주의 0.36%)을 단계적으로 선발할 수 있었다. 선발된 세균은 16S rRNA 유전자의 염기서열 분석을 통해 모두 Bacillus 속에 속하는 것으로 확인되었다. 이러한 결과로 선발된 7균주는 토마토의 고염류 또는 온도 스트레스에 대한 효과적인 미생물 제제로 활용이 가능한 것을 확인할 수 있었다. Salinity and extreme temperature stresses affect growth and productivity of crops negatively. Beneficial bacteria, including plant growth-promoting rhizobacteria (PGPR) induce growth promotion and tolerance of plants under abiotic stress conditions. In the present study, 20 strains were selected from 1944 isolated bacteria based on three plant growth-promoting (PGP) traits-aminocyclopropane-1-carboxylate deaminase activity, phosphate solubilization, indole-3-acetic acid production, and growth ability under salinity and extreme temperature stress conditions. Seven among the 20 strains were selected based on growth-promoting effects on plants under saline or temperature stresses in tomato plants. It was expected that the seven strains could induce tolerance of tomato plants under salinity or extreme temperature stresses, which implies that these seven strains can act as potential inducers of multiple stresses tolerance in tomato plants.

Bacillus aryabhattai H19-1에 의한 양파 생육후기 건조피해 완화 효과

문혜정,유성제,상미경 한국식물병리학회 2023 식물병연구 Vol.29 No.2

Climate change has occurred frequently, and it lead to a severe decrease in marketability and productivity. Onion has various functional elements related to antioxidant, antibacterial, anticancer and immune enhancement, the productivity is closely affected by climate conditions. In this study, to environmentally-friendly produce onions even though drought condition, effect of Bacillus aryabhattai H19-1 on the yield, marketability, and functional component of onions was evaluated in a field. When strain H19-1 was drenched, onion production and marketability significantly increased, and the contents of ascorbic acid and carotenoid of harvested onion bulbs also increased. Therefore, B. aryabhattai H19-1 is expected to be effective in enhancing onion productivity by mitigating drought stress.

산개성단 IC4665 내에 있는 변광성 빈도

김다운,백관혁,유성제,이혜인,선기영,박항,한수민,김희수 한국과학영재교육학회 2012 과학영재교육 Vol.4 No.2

The aim of this study was to investigate the variable star frequency in open cluster IC4665. For this, We observed the cluster using 4inch refrator telescope and CCD camera. After that, we searched variable star candidates. If the magnitude change of a star is larger than three times of standard deviation, we identified the star as a variable. we found that the varible star frequency of open cluster IC4665 is about 20%. 본 연구의 목적은 산개성단 IC4665를 CCD 관측을 수행하여 변광성을 찾는 것이다. 이를 위해 4인치 굴절망원경에 CCD 카메라를 연결하여 IC4665를 관측한 다음, 이 성단 내에 포함된 변광성을 찾았다. 성단내 구성원들의 변광성을 찾는 방법은 별의 밝기 변화가 관측의 허용오차보다 3배 이상 벗어난 별들을 변광성으로 판정하였다. 그 결과 측광한 93개의 성단 구성원 별들 중, 19개인 약 20%의 별이 변광성임을 알 수 있었다.

고추 식물의 건조 스트레스 완화를 위한 미생물 선발

김상태(Sang Tae Kim),유성제(Sung-Je Yoo),송재경(Jaekyeong Song),원항연(Hang-Yeon Weon),상미경(Mee Kyung Sang) 한국식물병리학회 2019 식물병연구 Vol.25 No.3

Drought stress is considered as one of major abiotic stresses; it leads to reduce plant growth and crop productivity. In this study, we selected bacterial strains for alleviating drought stress in chili pepper plants. As drought-tolerant bacteria, 28 among 447 strains were pre-selected by in vitro assays including growth in drought condition with polyethylene glycol and plant growth-promoting traits including production of 1-aminocyclopropane-1-carboxylate deaminase, indole-3-acetic acid and exopolysaccharide. Sequentially, 7 among pre-selected 28 strains were screened based on relative water content (RWC); GLC02 and KJ40, among seven strains were finally selected by RWC and malondialdehyde (MDA) in planta trials under an artificial drought condition by polyethylene glycol solution. Two strains GLC02 and KJ40 reduced drought stress in a natural drought condition as well as an artificial condition. Strains GLC02 or KJ40 increased shoot fresh weight, chlorophyll and stomatal conductance while they decreased MDA in chili pepper plants under a natural drought condition. However, two strains did not show biocontrol activity against diseases caused by Phytophthora capsici and Xanthomonas campestris pv. vesicatoria in chili pepper plants. Taken together, strains GLC02 or KJ40 can be used as bio-fertilizer for alleviation of drought stress in chili pepper plants.

Trichoderma sp. GL02에 의한 고추 식물의 건조 스트레스 완화 효과

김상태 ( Kim Sang Tae ),유성제 ( Yoo Sung-je ),송재경 ( Song Jaekyeong ),원항연 ( Weon Hang-yeon ),상미경 ( Sang Mee Kyung ) 한국유기농업학회 2020 韓國有機農業學會誌 Vol.28 No.3

Drought stress is one of major environmental stresses in plants; this leads to reduce plant growth and crop yield. In this study, we selected fungal isolate for mitigating drought stress in pepper plants. To do this, 41 fungi were isolated from rhizosphere or bulk soils of various plants in Jeju, Gangneung, Hampyeong in Korea. Out of 41 isolates, we screened two isolates without phytotoxicity through seed germination of tomato, pepper, and cabbage treated with fungal spores; through following plant assay, we selected GL02 as a candidate for alleviating drought stress in pepper plants. As a result of greenhouse test of pepper plants in drought condition, the stomatal conductance on leaves of pepper plants treated with GL02 was increased, whereras the malondialdehyde (MDA) and electrolyte leakage were decreased compared to that in control plants. When stressed plants were rewatered, stomatal conductance of the plants treated with GL02 was increased; the electrolyte leakage was decreased. Based on internal transcribed spacer (ITS) sequencing analysis, isolate GL02 was belonging to genus Trichoderma. Taken together, drought stress in pepper plants treated with GL02 was alleviated, when it was rewatered after drought-stressed, the plants could be recovered effectively. Therefore, Trichoderma sp. GL02 could be used as a bio-fertilizer to alleviate drought stress in pepper plants.

바코드스캐너를 활용한 스마트 쇼핑(Smart Shopping)

김성호,이유상,유병석,유성제 한국산업경영시스템학회 2013 한국산업경영시스템학회 학술대회 Vol.2013 No.추계