학교체육경영에 있어서 교사의 직무태도에 영향을 미치는 요인분석

원기승,김경호,김영재 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this paper two to find out elements which have an effect on teacher's behavior and job satisfaction/dissatisfaction on physical education services in the managerial organization. These elements were called "Determining Factors of Job Accomplishment", and divided into immanent and external side in job. The former was defined as a sense that teachers have directly throughout job accomplishment, and the latter meant the organizational context of teacher's job accomplishment. It was assumed that the relation between some organizational elements and teacher's behavior would be conditioned by contingency variables as follow: types of physical education service, sex. Data were collected from 138 teachers in elementary schools and lower secondary schools by questionnaires in the fall of 2003. The summary of the results was: 1) In every of service job immanent side affected the positiveness of job accomplishment behavior. 2) Any categories of job external side have no effect on teacher's behaviors in Club Service(C.S). While in Program Service(P.S.) 4 categories: teachers group, principal, chief of physical education teachers, physical education service, related to daily behavior. And in addition to these 4 categories well-filled material condition was important in Area Service(A.S.). 3) Female teacher was activated only by job immanent side, while male teacher's activity was influenced by both sides. It was suggested that as to organizational power, the male accepted more compliantly than the female. 4) High job satisfaction exalted the willingness to job accomplishment but job dissatisfaction did not have relation to in it every services. 5) About the same factors provided for job satisfaction and dissatisfaction in C.S. but determining factors of both feelings to job were different in P.S., that is, job immanent side and the policy of services contributed to job satisfaction, fully equipped social and physical conditions reduced dissatisfaction.

학교체육 경영 활성화를 규정하는 조직풍토요인의 분석 : In the Case of the Managerial Organization for School Physical Education

원기승,문행자,최경범 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This paper refered to the managerial organization for school physical education. But reports of this type of study have apparently not been published to date. Then in this paper it was indicated that the managerial organization should be discussed from three view points; (1) correlation between the school organization and managerial organization for physical education. (2) contingency theory. (3) motivational approach. The purposes of this paper. therefore, were to clarify the structure of the organizational climate and to analysis the relation between the organizational climate and the effectiveness of managerial organization. The survey was conducted at 7 primary school and 6 junior high school by questionnaire, and the subjects were 312 teachers. The summary of the results were: 1) The organizational climate of a school was consisted of six clements as follow: Maintenance, Cooperation, Task Achievement Job efficiency, Disengagement. Hindrance. These elements were integrated two dimensions that were HUMA-SYSTEM and TECHNICAL-SYSTEM. 2) The correlation between the organizational climate and Integration-Latency function of the managerial organization was significant high. Especially the organizational identification of the managerial organization was significant high. Especially the organizational identification which was one of the effectiveness variables was influenced most strongly by HUMAN and TECHNICAL-SYSTEM dimensions. 3) The positive attitude toward physical education services of teachers created innovation of management for physical education.

새로운 국산 V-Tube 진공채혈관과 BD Vacutainer의 비교평가

원은정,신명근,장민중,조덕,기승정,김수현,신종희,원용관,양동욱,서순팔 대한진단검사의학회 2013 Laboratory Medicine Online Vol.3 No.3

Background: Vacuum tubes are widely used in clinical laboratories for routine tests. We compared a newly developed V-tube (AB Medical, Korea)and BD tubes (BD, USA) in common clinical assays, i.e., hematological, chemical, and immunological tests. Methods: In total, 100 volunteers comprising 79 patients and 21 healthy volunteers were recruited and peripheral blood samples were collected with 2 brands of EDTA tubes and serum-separating tubes (SSTs). EDTA-tube samples were evaluated using 16 routine hematological tests. The SST samples were analyzed for 32 routine chemical parameters and 3 thyroid hormones. The results were statistically analyzed using the paired ttest and Bland-Altman plot. In addition, the stability of each analyte in 2 brands of vacutainers was evaluated. The results of the hematological tests at t=0 hr were compared with those at t=72±2 hr, and the results of the chemical parameters and thyroid hormones at t=0 hr were compared with those at t=72±2 hr and t=168±2 hr for each tube. Results: Paired t-test analysis revealed that the test results of 16 routine hematological parameters, 32 routine chemical parameters, and 3 thyroid hormones showed clinically allowable differences between the 2 brands of vacuum tubes (t =0 hr). The results obtained when using V-tubes showed a statistically significant correlation with those obtained when using BD tubes. The stability of each analyte was similar in both vacuum tubes. Except for 10 parameters (white blood cell count, mean corpuscular volume, basophils [ %], mean corpuscular hemoglobin concentration,monocytes [ %], phospholipid, sodium, potassium, chloride, and free T4), all parameters showed significant but clinically allowable differences with regard to storage duration. Conclusions: The newly developed V-tube vacutainers provide a suitable alternative to BD tubes in common clinical laboratories. 배경: 진공채혈관은 임상검사에서 널리 쓰이고 있다. 본 연구자들은 최근 개발된 V-tube와 BD Vacutainer 진공채혈관을 일반혈액검사, 일반화학검사 및 면역학적 검사를 대상으로 비교 평가하였다. 방법: 총 100명의 성인(건강인 21명, 환자 79명)을 대상으로 본 연구를 시행하였다. 말초혈액을 ethylene diamine tetra acetic acid(EDTA) tube와 serum separator tube (SST) 각각에 채혈하여 두 진공채혈관을 비교하였다. EDTA tube로는 일반혈액검사 16종을, SST로는 일반화학 32종 및 갑상선 기능검사 3종에 대하여 검사를 시행하였다. 두 진공채혈관의 검사 결과는 쌍체비교법과 Bland-Altmanplot을 이용해 비교하였다. 안정성 검사를 위해서 두 진공채혈관 각각의 채혈당일 검사결과를 기준으로 하여 일반혈액검사는 채혈 후3일째의 검사치의 변화를, 일반화학 및 갑상선기능검사는 채혈 후3일, 7일째의 검사치의 변화를 검사 항목별로 분석하였다. 결과: 당일 검체로 시행한 두 진공채혈관의 측정값 간 차이는 일반혈액검사 16종, 일반화학검사 32종, 갑상선기능검사 3종 모두에서높은 일치도를 보였고, 임상적으로 허용 가능한 범위 내에 속하였다. 또한 두 진공채혈관의 측정값 간에는 통계학적으로 유의한 강한 양의 상관관계를 보였다. 보관 기간에 따른 검사 안정성 비교에서 두 진공채혈관 모두 유사한 정도의 안정성을 보였다. 당일 검체와 3일째 검체의 비교 시 WBC, MCV, Basophil은 두 채혈관 모두에서 임상적으로 유의한 차이를 보였고, BD 진공채혈관에서는MCHC, Monocyte에서 임상적으로 유의한 차이를 보였다. 일반화학검사와 갑상선 기능검사의 경우 의 경우 당일 검체와 보관 검체(3일, 7일)와의 결과 비교 시 두 가지 진공채혈관 모두에서 대부분의 항목의 결과가 통계적으로 유의한 차이가 있음을 알 수 있었다. 하지만 PL, Na, K, Cl, free T4 항목을 제외한 대부분은 임상적으로허용할 만한 오차 범위 내의 결과를 보였다. 결론: 임상검사실에서 흔히 시행하는 검사들에 대해 새로 개발된V-tube를 BD Vacutainer 진공채혈관과 비교 평가한 결과 국산 Vtube진공채혈관이 수입제품을 대체할 수 있을 정도의 품질과 성능을 보유한 것으로 평가되었다.

학교체육 경영에 있어서 교사의 직무태도에 영향을 미치는요인 분석

김영재,원기승,김경호 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

Internal Quality Assurance Status of Stool Examination as Assessed by a Questionnaire in Korean Clinical Laboratories

권용준,원은정,기승정,김수현,신명근,신종희,서순팔 대한진단검사의학회 2018 Laboratory Medicine Online Vol.8 No.1

This study aimed to survey the status of quality control (QC) assurance for stool examinations at clinical laboratories in Korea. We sent a questionnaire related to QC practices in stool examination by electronic mail to Korean clinical laboratories that performed stool examination. Overall, 20 of the 39 laboratories (51.3%) reported performing stool concentration methods, and 28 (71.8%) examined the slides using only saline. A large proportion (74.4%) of respondents did not check the internal QC because of the restriction of appropriate control materials. Only four laboratories (10.3%) checked the reactivity of the dye solution routinely. For appropriate external QC systems, QC slides (43.6%) were preferred, followed by QC materials (30.8%), virtual slides (17.9%), and a combination of the above options (7.7%). The most commonly observed parasites in stool samples at the clinical laboratories were Clonorchis sinensis (75%), followed by Endolimax nana, Enterobius vermicularis, and Entamoeba coli. The present study describes the difficulties in internal QC assessment due to the absence of standardized QC materials and systems. We hope the findings of this report will provide a foundation for a QC assessment program for stool examinations in the near future.

자동화학분석기를 이용한 인도시아닌 그린(ICG) R15 검사의 수행능 평가

박주헌,원은정,최현정,기승정,서순팔 대한진단검사의학회 2016 Laboratory Medicine Online Vol.6 No.3

Background: The conventional indocyanine green retention rate at 15 minutes (ICG R15) test is inefficient and inconvenient because it requires the use of a manual spectrophotometer and several samples per patient. This study aimed to establish the automation of the ICG R15 test using an automated clinical chemistry analyzer, and to evaluate the calculation of R15 with a small number of samples. Methods: The performance of the AU5832 (Beckman Coulter, USA) for determining ICG concentration was evaluated in accordance with the Clinical Laboratory Standards Institute (CLSI) guidelines. The R15 results for 77 patients determined by spectrophotometry and AU5832 were compared. We evaluated the calculation of R15 with three samples, except for one sample in which the results had been obtained previously, at 5, 10, and 15 minutes after injection of ICG into the patients, and compared the results with those obtained with four samples. Results: The automated ICG test using the AU5832 system showed proper performances according to CLSI. Although the difference in the R15 results between the two methods was within the 95% confidence interval, the R15 was adjusted by the regression equation because it was slightly lower according to the automated method compared with the manual method. The R15 with three samples (0, 5, and 15 minutes) showed the best correlation with conventional R15 with four samples (r2=0.996). Compared with the manual method, the R15 result using the AU5832 showed excellent agreement with four samples (kappa value 0.904) and with three samples (kappa value 0.880). Conclusions: The ICG R15 test using the AU5832 system is comparable with the conventional method in clinical use. 배경: 기존 인도시아닌 그린 15분 정체율(Indocyanine green retention rate at 15 minutes, ICG R15) 검사는 분광광도계를 이용한 수기법 검사이며, 한 환자에서 여러 개의 검체를 필요로 하는 불편함이 있었다. 본 연구에서는 자동화학분석기를 이용한 ICG R15 자동화 검사를 구축하고자 하였으며, 또한 기존보다 적은 수의 검체를 이용하여 ICG R15 검사가 가능한지 알아보고자 하였다. 방법: AU5832 (Beckman Coulter, USA) 자동화학분석기를 이용한 ICG 농도 측정능 평가는 CLSI 지침에 따라 시행하였다. R15 결과의 수기법과 자동화 검사법 간 상관성 비교는 77명의 환자 검체를 이용하였다. ICG 주입 전, 주입 후 5분, 10분 및 15분에 채취한 검체 중 한 개를 제외한 3개의 검체만을 이용하여 R15를 산정할 수 있는지 4개의 검체 모두를 이용하여 산정한 R15와 비교하였다. 방법 간의 일치도는 R15 결과를 임상적 적용에 따라 세 가지 범주로 분류하여 평가하였다. 결과: AU5832를 이용한 ICG 농도 측정 자동화 검사는 CLSI 지침의 기준에 합당하는 좋은 측정능을 보였다. 수기법의 R15를 기준으로 자동화학검사법의 편차는 대부분 95% 구간 내에 속했으나 다소 낮게 편향되어 두 결과 간 회귀방정식으로 자동화 검사법의 R15를 보정하였다. 3개의 검체(0분, 5분, 15분)로 R15를 산정하였을 때, 기존 4개의 검체로 산정한 R15와 가장 높은 상관성을 보였다(r2=0.996). 수기법의 R15와 비교하여 자동화 검사법의 R15는 4개의 검체(kappa value 0.904) 또는 3개의 검체(kappa value 0.880)를 이용하여도 좋은 일치도를 보였다. 결론: AU5832 자동화학분석기를 이용한 ICG 농도 측정능은 CLSI 기준에 합당하였으며, R15 검사 성적 역시 수기법과 유의한 차이가 없었다. 따라서 AU5832를 이용한 ICG R15 검사와 3개의 검체만을 이용한 ICG R15 검사는 임상적으로도 기존 방법과 동일하게 이용될 수 있을 것으로 사료된다.

Comparison of AdvanSure TB/NTM PCR and COBAS TaqMan MTB PCR for Detection of Mycobacterium tuberculosis Complex in Routine Clinical Practice

조원형,원은정,최현정,기승정,신종희,양동욱,서순팔 대한진단검사의학회 2015 Annals of Laboratory Medicine Vol.35 No.3

The AdvanSure tuberculosis/non-tuberculous mycobacterium (TB/NTM) PCR (LG Life Science, Korea) and COBAS TaqMan Mycobacterium tuberculosis (MTB) PCR (Roche Diagnostics, USA) are commonly used in clinical microbiology laboratories. We aimed to evaluate these two commercial real-time PCR assays for detection of MTB in a large set of clinical samples over a two-year period. AdvanSure TB/NTM PCR and COBAS TaqMan MTB PCR were performed on 9,119 (75.2%) and 3,010 (24.8%) of 12,129 (9,728 respiratory and 2,401 non-respiratory) MTB specimens, with 361 (4.0%) and 102 (3.4%) acid-fast bacilli (AFB)-positive results, respectively. In MTB culture, 788 (6.5%) MTB and 514 (4.2%) NTM were identified. The total sensitivity and specificity of the AdvanSure assay were 67.8% (95% confidence interval [CI], 63.9-71.6) and 98.3% (95% CI, 98.0-98.6), while those of the COBAS TaqMan assay were 67.2% (95% CI, 60.0-73.8) and 98.4% (95% CI, 97.9-98.9), respectively. The sensitivities and specificities of the AdvanSure and COBAS TaqMan assays for AFB-positive and AFB-negative samples were comparable. Furthermore, the AdvanSure assay showed fewer invalid results compared with the COBAS TaqMan assay (5.0 vs. 20.4 invalid results/1,000 tests, P<0.001). AdvanSure assay represents a comparable yet more reliable method than COBAS TaqMan for the identification of mycobacteria in routine clinical microbiology.

Comparison of the Bruker Biotyper and VITEK MS Matrix- Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Systems Using a Formic Acid Extraction Method to Identify Common and Uncommon Yeast Isolates

이현승,신종희,최민지,원은정,기승정,김수현,신명근,서순팔 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.3

Background: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with MALDI Biotyper, but the direct on-plate FA extraction is simpler. We compared the Biotyper with the VITEK MS for the identification of various clinically relevant yeast species, focusing on the use of the FA extraction method. Methods: We analyzed 309 clinical isolates of 42 yeast species (four common Candida species, Cryptococcus neoformans, and 37 uncommon yeast species) using the Biotyper and VITEK MS systems. FA extraction was used initially for all isolates. If ‘no identification’ result was obtained following the initial FA extraction, these samples were then retested by using FA (both systems, additive FA) or FA/ACN (Biotyper only, additive FA/ACN) extraction. These results were compared with those obtained by sequence-based identification. Results: Both systems correctly identified all 158 isolates of the four common Candida species after the initial FA extraction. The Biotyper correctly identified 8.7%, 30.4%, and 100% of 23 C. neoformans isolates after performing initial FA, additive FA, and FA/ACN extractions, respectively, while VITEK MS identified all C. neoformans isolates after the initial FA extraction. Both systems had comparable identification rates of 37 uncommon yeast species (128 isolates), following the initial FA (Biotyper, 74.2%; VITEK MS, 73.4%) or additive FA (Biotyper, 82.0%; VITEK MS, 73.4%). Conclusions: The identification rate of most common and uncommon yeast isolates is comparable between simple FA extraction/Biotyper method and VITEK MS methods, but FA/ ACN extraction is necessary for C. neoformans identification by Biotyper.

Identification of Candida auris and Closely Related Species Using a New Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, the ASTA MicroIDSys System

마태오,인용하,변승아,이가영,최민지,이승엽,원은정,최현정,최현우,기승정,김수현,신명근,신종희 대한진단검사의학회 2022 Laboratory Medicine Online Vol.12 No.1

Candida auris is a multidrug-resistant fungal pathogen emerging worldwide that is closely related to the C. haemulonii species complex. The ASTA MicroIDSys (ASTA, Korea) is a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system developed for species-level identification of microorganisms. However, prior to the current study, the reference database of ASTA MicroIDSys did not include C. auris. We expanded the database by adding 20 reference strains of C. auris and three closely related species belonging to C. haemulonii species complex. Further, we compared the performance of the ASTA system using an expanded database (coreDB v1.27.02) to that of the Biotyper system (Bruker Daltonics, USA) using 91 well-characterized Candida isolates from a Korean collection. In addition, we evaluated the ability of the ASTA system to differentiate between clade II and non-clade II isolates of C. auris. The results revealed that both ASTA and Biotyper systems accurately identified all 73 C. auris isolates. Of the 18 isolates of closely related species (nine C. pseudohaemulonii, seven C. haemulonii, and two C. haemulonii var. vulnera), the ASTA and Biotyper systems correctly identified 16 and 14 isolates, respectively, to the species level. Neither system misidentified any of the 91 isolates. Cluster analyses of the ASTA spectra distinctly discriminated clade II Korean C. auris isolates from the non-clade II isolates obtained from other countries. Our results show that the ASTA system with an expanded database is a reliable platform for the identification of C. auris and closely related species.