이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화

우현숙,이상훈,이동기,김진수,원성혜,이병현,Woo, Hyun-Sook,Lee, Sang-Hoon,Lee, Dong-Gi,Kim, Jin-Soo,Won, Sung-Hye,Lee, Byung-Hyun 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

이탈리안 라이그래스의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스 유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 5mg/L 2.4-D가 첨가된 MS배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 5mg/L BA가 첨가된 N6배지에서 배양했을 때 70%이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 배발생 캘러스의 형성율은 품종간에 큰 차이가 없었으나, 재분화 능력은 Jeanne 품종이 73%의 가장 높은 재분화율을 나타내어 품종간에 큰 차이를 나타내었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 이탈리안 라이그래스의 개발에 유용하게 응용되어질 수 있을 것이다. As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

리그닌 생합성관련 유전자의 발현조절에 의한 고품질 목초 개발

우현숙(Hyun-Sook Woo),윤정우(Jungwoo Yun),이병현(Byung-Hyun Lee) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.1

To develop a new variety of orchardgrass with improved digestibility, caffeic acid O-methyltransferase (Dgcomt), which is a methylation enzyme involved in the early stages of lignin biosynthesis, was isolated and characterized. Dgcomt was expressed not only in leaves but also in stems and roots. The expression levels of transcripts were high in stems and roots which are the most lignified tissues, and only moderate levels of transcripts were expressed in leaves. To develop transgenic orchardgrass plants by down-regulating the Dgcomt gene, an RNAi suppression vector with partial Dgcomt DNA fragment was constructed and transferred into the genome of orchardgrass via Agrobacterium-mediated gene transfer method. PCR and Southern blot analyses with genomic DNAs from putative transgenic plants revealed that the T-DNA region containing RNAi construct was successfully integrated into the genome of orchardgrass. Northern blot analysis revealed that the majority of the down-regulated transgenic lines showed significant reduction in Dgcomt gene expression. These RNAi transgenic orchardgrass will be useful for molecular breeding of new variety with improved digestibility by down-regulating lignin biosynthetic enzyme.

생활환경 문제 해결을 위한 초등 탐구 중심 환경교육 교수·학습 방안

우현숙(Woo, Hyun Sook),이상원(Lee, Sang Won) 한국실과교육학회 2020 한국실과교육학회지 Vol.33 No.1

이 연구는 초등환경교육의 교수·학습 방법으로 탐구 중심 환경교육을 활용하여 초등학생을 대상으로 생활환경 문제 해결을 위한 교육프로그램을 개발함으로써 초등학교 현장에서 환경교육을 효과적으로 구현하고자 수행하였다. 연구의 목적을 달성하기 위하여 2009 개정교육과정을 반영한 5∼6학년(군) 실과 6종 교과서의 환경교육 관련 내용과 2015 개정교육과정 실과총론의 환경교육 관련 내용을 분석하여 환경교육 내용영역과 시사점을 추출하였으며, 이를 토대로 초등 환경교육을 위한 탐구 중심 환경교육 프로그램을 개발하였다. 개발된 프로그램은 준비-탐구 계획서 작성-탐구 수행 및 결과 분석-통찰적 이해-탐구의 확장의 5단계 모형을 구안하였다. 개발된 교수·학습 프로그램을 실과 교과 시간을 통해 지도하고, 환경 주제 중심으로 교과 간에 통합하여 창의적 체험활동 시간에 활용하거나 환경 동아리 시간에 활용할 수 있는 실행 방안을 제시하여 수업현장에서 효과적으로 활용할 수 있도록 하였다. 이 연구를 통해 얻은 결론은 다음과 같다. 첫째, 5∼6학년(군) 실과 교과서의 환경교육 관련 내용을 권진희 외(2017)의 전통생태지식을 활용한 초등환경교육 분류 체계를 바탕으로 환경 주제를 선정하고, 탐구 중심 환경교육 프로그램을 개발하였다. 둘째, 개발된 탐구 중심 환경교육 프로그램은 실과 시간, 창의적 체험활동시간, 환경 동아리 활동 시간에 활용할 수 있다. 셋째, 탐구 중심 환경교육을 활용한 초등환경교육은 학습자 중심의 능동적인 학습을 이끌어 내어 환경에 대한 학습자의 관심과 동기를 유발하고, 일상적인 생활환경 문제에 대한 관심과 타인과의 의사소통을 통해 창의적인 문제 해결력을 향상시키는데도 기여를 할 것이다. 결론적으로 탐구 중심 환경교육을 활용한 환경교육은 학습자 중심의 능동적인 학습 활동을 통해 문제를 해결하는 과정을 통해 고등 사고력과 창의적 역량을 기를 수 있는 좋은 예시자료가 될 수 있을 것으로 보인다. The purpose of this study is to contribute to implement effective environmental education at the elementary school by developing educational programs for elementary school students to solve their living environment problems by using inquiry-based environmental education through teaching and learning method for elementary environmental education. To achieve the purpose of the study, the environmental education related contents of the five to six grade and six types of practical arts textbook reflecting the 2009 revised elementary school curriculum and the 2015 revised elementary school practical arts curriculum were analyzed. Based on those, the contents of environment education and its implications were extracted, and the inquiry-based environmental education program was developed for elementary environmental education. The conclusions obtained from this study is as follows. First, environmental subjects were selected based on the classification system for elementary environmental education using traditional ecology knowledge of Kwon et al.,(2017) and the inquiry-based environmental education program was developed. Second, the inquiry-based environmental education programs can be used for regular classes hours, creative experiential activities hours and environmental club activities. Third, the elementary inquiry- based environmental education program will be effective in achieving the goal of environmental education by bringing active learning to the learner-centered, inducing learner s interest and motivation to the environment, and will also contribute to improving their creative problem-solving skills through daily life environment problems and communication with others. In conclusion, environmental education using inquiry-based environmental education can be a good example of developing higher thinking skills and creative capabilities through the active learner-centered learning.

톨 페스큐의 성숙종자로부터 효율적인 캘러스 배양 및 식물체 재분화

김도현,이동기,이상훈,우현숙,이기원,최명석,이병현,Kim Do-Hyun,Lee Dong-Gi,Lee Sang-Hoon,Woo Hyun-Sook,Lee Ki-Won,Choi Myung-Suk,Lee Byung-Hyun 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.3

In an effort to optimize tissue culture conditions for genetic transformation of tall fescue (Festuca arundinacea Schreb.), an efficient plant regeneration system from seed-derived calli was established. MS medium containing 6 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L benzyladenine (BA) were optimal for embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency above 50% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. 'Kentucky-31' showed to have high frequencies of embryogenic callus induction and plant regeneration up to 58.3 and 50%, respectively. Addition of sucrose to the regeneration medium as a carbon source increased regeneration frequency up to 55%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of tall fescue through genetic transformation. 톨 페스큐의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스는 6 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS배지에서 가장 높은 빈도로 유도되었으며, 식물체 재분화율도 증가시키는 것으로 나타났다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 N6배지에서 배양했을 때 50% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 품종간 배발생 캘러스의 형성율과 식물체 재분화율은 Kentucky-31 품종이 각각 58.3%와 52%로서 가장 높은 효율을 나타내어 품종 간에 큰 차이를 나타내었다. 탄소원으로는 sucrose를 첨가해주었을 때 식물체 재분화율이 55%로 증가되었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 톨 페스큐의 개발에 유용하게 응용되어질 수 있을 것이다.

오차드그래스의 종자배양에 있어서 식물생장조절물질과 항산화제가 캘러스유도와 식물체 재분화에 미치는 영향

이기원(Ki-Won Lee),이상훈(Sang-Hoon Lee),이동기(Dong-Gi Lee),우현숙(Hyun-Sook Woo),김도현(Do-Hyun Kim),최명석(Myung Suk Choi),김기용(Ki Young Kim),이효신(Hyoshin Lee),이병현(Byung-Hyun Lee) 한국초지조사료학회 2005 한국초지조사료학회지 Vol.25 No.3

이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화

우현숙,이상훈,이동기,김진수,원성혜,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media tested, MS and M6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.