결합조직형성 모낭상피종

오칠환(Chil Hwan Oh),김수남(Soo Nam Kim) 대한피부과학회 1986 대한피부과학회지 Vol.24 No.4

We present a case of desmoplastic trichoepithelioma in a 21-year-old woman. She had a asymptomatic hard annular plaque with a raised border and depresaed center on the chin. Histopathological findings showed narrow strands of basaloid cell, keratinous cyst and desmoplastic stroma, the features corresponding to the desmoplaslic trichoepithelioma.

영상분석법을 이용한 노화에 따른 피부주름의 변화에 대한 연구

최현철,오칠환 ( Hyun Chul Choi,Chil Hwan Oh ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Background: The confgurations of the skin surface, as characterized by surface furrows and plateaux, are known to change with ageing. There are many parameters to analyze the effects of ageing processes on the surface configuration. However, there are contradictory results among the authors. Objective : The purpose of this study was to compare the sensitivity of various parameters being described in other reports with our new parameters used in evaluating the changes of the skin surface in the agein processes. Methods : 112 healthy Korean males in 4 age groups participated in this study. Silicon skin replicas were taken from medial and lateral aspects of the forearm. Images of the skin replicas were analyzed by means of a digital image processing system(AIC, GA). Most parameters were calculated and measured in the primary skin axis. Image analyses were porformed by 3 different processes i.e., morphometry, densitometry(optical profilometry), and histograms. Results.In Morphometry, the furrow count in the vertical section(VFC), the coefficient of the developed skin surface(CDSS) and mean width at maximal interception(MWMI) showed a tendencies to decrease with ageing.Irregularity of shadow shape(ISS) showed a tendency to increase with ageing. In Densitometry, the mean surface roughness(Ra) and mean depth of roughness(Rz) showed a tendency to increase with ageing. On histogram analysis, skewness was decreased with ageing but kurtosis did not show any significant changes. Conclusion :Ra, Rz, and ISS have shown a tendency to increase in the ageing processes(p<0. 05). VFC, CDSS, MWMI and skewness showed tendencies to decrease with ageing but the results were not statistically significant. The different results between morphometry and densitometry are likely to be due to increrr ent angles in the skin furrows with ageing. (Kor J Dermatol 1997;35(2): 292-302)

아토피 피부염 환자 어머니의 정서상태 및 가족기능에 관한 예비적 연구

정휘정,오칠환,조숙행 ( Hwee Jeong Cheong,Chil Hwan Oh,Sook Haeng Joe ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

영상분석법을 이용한 비만세포의 정량적 연구

김일환 ( Il Hwan Kim ),오용섭 ( Yong Sub Oh ),홍진호 ( Jin Ho Hong ),송해준 ( Hae Jun Song ),오칠환 ( Chil Hwan Oh ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Background: The presence of mast cells often in increased numbers, has been noted in various cutaneous disorders. Recent studies suggest that mast cells are of primary importance in these conditions and their presence does not merely represent a second event. Previous reports of mast cell enumeration in normal and pathologic tissues have revealed inconsistent results, reflecting technical difficulties in quantitation. Objective : The purpose of our study is to develop a new approach for quantifying mast cell in tissue section that is accurate, reproducible and avoids problems caused by the variation in distribution of these cells in the tissue. Methods : Mast. cells were quantitated in eight cases of mastocytosis and in the flank and back skin of three normal human controls in toluidine blue stain using a computerized image analysis system. Results : Macular and nodular mastocytosis patients had from twenty-fold to nearly a 100-fold greater mast cell content than was observed in normal skin. Conclusion : Image analysis in conjunction with toluidine blue stain offers a single and accurate method for mast cell quantification. This technique also provides a practical and important new diagnostic tool for establishing the presence of increased lesional skin mast cells in patients with mastocytosis. (Kor J Dermatol 1996;34(2): 194-198)

부속이 3예

조창근,김일환,오칠환 ( Chang Geun Cho,Ill Hwan Kim,Chil Hwan Oh ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.5

Accessory tragi are rare congenital malformations of the external ear. They are present from birth and appear as solitary or multiple papules located between the pretragal and sternoclavicular regions. Accessory tragi may be multiple but are almost always unilateral and rarely bilateral. We report two cases of bilateral accessory tragus and a typical case of unilat,eral accessory tragus manifesting as skin-colored papules on the preauricular area. (Korean J Dermatol 1997;35(5): 980-983)

멜라닌 세포성 병변에서 화상분석법을 이용한 DNA 배수성에 관한 연구

이길주,김동준,오칠환 ( Gil Ju Yi,Dong Jun Kim,Chil Hwan Oh ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Backgroutld: In the cutaneou; neoplasms, especially melanocytic skin lesions, the degree of t.he nuclear atypia such as the hyp rchromatism, pleomorphism, and giantism is a very impor tant factor when determining the differntial diagnosis between the malignant. and the benign turnors and the prognosis. Objective : So we measured and cornpared the nuclear atypia of the malignant melanoma, and acquired and congenital melanocytic nevi(AMN and CMN) using the computerized image analysis (IA). Methods : Five micron ser,t ons were cut from each paraffin block. The sections were Feulgen-stained and image analysis was perforrned with an AIC image analysis system. In each case, 50 lymphocytes nuclei fnim the sarne specimen slide were measured as an internal diploid control. Each 100 cells of the MM, CMN and AMN were measured and are referred to in this report as the upper part and the lower part. The morphological parameters such as mean nuclear area, coefficient of variation(CV) of the nuclear area, roundness of the nucleus and CV of the roundness were measured. For the nuclear content, mean ploidy, CV of the mean ploidy ind 2c Deviation Index(2cI3I) were calculated and then the DNA histograms were produced. Results : Nuclear area, CV of the nuclear area, mean ploidy, CV of the mean ploidy and 2cDI were found to be significantly reat.er for rnalignant melanoma than for CMN and AMN. The nuclear area of AMN is significantly lar ger than that of CMN and there were no significant differences between the uper and the lower part.s of three melanocytic lesions in any of the measured parameters. Conclusion . The results of o ir study suggest that the IA of the melanocytic cells might reflect the biologic behavior rnore sensitively t,han do clinical or histologic criteria, therefore important information for differentiating thi benign and the malignant melanocytic lesions may be obtained by the DNA profile and morphomi try on Feulgen st,ained tissue specirnens using IA.(Korean J Dermatol 1997;35(6): 10591065)

에크린 혈관종성 과오종 2예

유화정 ( Hwa Jung Ryu ),오칠환 ( Chil Hwan Oh ),김일환 ( Il Hwan Kim ) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.11

Eccrine angiomatous hamartoma is a rare benign tumor similar to hemangioma in appearance. Unlike hemangioma, it has numerous eccrine structures in addition to numerous capillary channels. Occasionally it accompanies hyperhidrosis and pain. The sweat apparatuses in the lesions showed antigenic activities by immunohistochemical stains, particularly with anti-S100 protein and anti-CEA, the two common antigens associated with eccrine sweat glands. The antigenic activities of anti-S100 protein and anti-CEA in sweat gland structure of 2 presenting cases, one with sweating and the other without sweating, were compared to each other. As a result, we found no differences between the two cases. Although an aggressive treatment is not required due to its benign nature, total excision can be considered in cases with severe symptoms. We present two cases of eccrine angiomatous hamartoma developed on right knee joint areas in a 2-year-old male and a 10 month-old male, respectively.

절삭유 취급 근로자의 피부질환에 관한 연구

천병철,김희옥,김순덕,오칠환,염용태,Chun, Byung-Chul,Kim, Hee-Ok,Kim, Soon-Duck,Oh, Chil-Hwan,Yum, Yong-Tae 대한예방의학회 1996 예방의학회지 Vol.29 No.4

절삭유의 사용과 피부질환의 관계를 알아보기 위하여 한 자동차공장의 엔진 및 구동부서 근로자 1,004명을 대상으로 피부 이상 증세에 대한 기초설문조사를 실시하여 응답을 해준 근로자 667명을 대상으로 분석 및 피부과적 검진을 실시하였다. 또한 절삭유의 노출 정도를 알기 위한 방법으로 공기 중 오일 미스트를 측정하였다. 이 조사의 결과를 요약하면 다음과 같다. 1. 공기 중 오일미스트는 3개 부서로 나누어서 측정 하였는데, 전체 52건의 측정건수 중에서 8시간가중 평균 기준치인 $5mg/m^3$을 넘는 건수는 9건(17.3%)이었고, 로그정규분포상에서 95% 신뢰한계의 전체 상한치는 기준치 미만이었다. 2. 조사한 부서는 엔진가공부(258명), 가솔린 엔진조립부(210명), 디젤엔진조립부(96명), 구동부 가공반(86명), 구동부 조립반(17명)의 5개 부서였으며, 조사한 667명의 평균 나이는 34.5세였고, 근무연수의 중앙값은 7년 7개월이었다. 3. 이들 부서 중 엔진가공부와 구동부 가공반(A군)은 절삭유를 공정상 많이 사용하는 부서로 근로자 344명 중 298명 (86.6%)이 한 가지 이상의 절삭유를 사용하고 있는 반면, 가솔린엔진 조립부, 디젤엔진 조립부, 구동부 조립반의 근로자 323명(B군) 중에는 31명 (9.1%)만이 절삭유를 사용하고 있었다. 4. 피부 이상 증세의 경험자 227명 중 213명에 대하여 피부과전문의의 진찰을 시행하였는데, 이중에 172명이 검진당시 증세를 호소했는데, 1인당 평균 호소증세 건수가 1.5건 정도였다. 가장 많은 증세는 가려움증(32.5%), 구진(21.6%), 인설(15.7%), 소수포(12.5%), 반점(9.4%)의 순서였다. 5. 검진을 받은 근로자 231명 중 정상소견이나 뚜렷한 병변이 없는 사람으로 분류된 경우는 53명이었고, 나머지 160명에서 피부과적 질환이 발견되었다. 가장 많은 것은 접촉 피부염으로 전체 질환자 중 92명 (57.5%)을 차지하였고, 이들 중에는 여드름이나 낭포염 등 기타 다른 피부질환을 동반한 경우가 23명 (25%) 이었다. 기타 낭포염, 여드름만 있는 경우도 각각 9명(5.6%), 8명(5.0%)이었으며 , 각질화와 색소 이상도 1명 씩 발견되었다. 기타의 수부 습진은 비특이성 수부습진으로 분류되었는데 이들은 47명이었다. 6.피부질환이 있다고 진단받은 160명에서 발견된 피부과적 이상부위는 1인당 1.3부위였다. 이상이 발견된 부위는 손바닥(27.6%), 손가락과 손톱(22.7%), 상박부(16.0%), 손등(11.8%), 손가락 사이(10.3%)의 순이었고, 이들 수부 및 상박부위의 이상이 전체 이상의 약 88.7%였다. 7. 전체 피부질환의 유병상태는 각 부서별로 차이가 많았는데, 특히 접촉 피부염의 경우 엔진가공부는 1,000명당 213명, 가솔린엔진 조립부는 1,000명당 29명, 디젤엔진 조립부는 1,000명당 73명이었고, 구동부 가공반은 1,000명당 267명, 구동부 조립반은 1,000명당 59명으로 부서별로 차이가 매우 켰다. 이러한 경향은 절삭유 사용에 따라서 특히 수용성 절삭유만 사용하는 군과 두 종류의 절삭유를 모두 사용하는 근로자에서 마찬가지로 높게 관찰되었다. 8. 감별진단이 필요하다고 판단된 접촉 피부염 환자 49명은 첩포 검사를 시행하였다. 첩포 검사 결과, 기본 표준시료에 양성이 나타난사람이 12명, 기본표준시료와 오일시료에 모두 양성이 나타난 사람 3명, 그리고 오일 시료에만 양성이 나타난 사람 3명이 있었고, 기본 표준시료와 유기용제시료에 양성이 나타난 사람, 유기용제 시료에만 양성이 나타난 사람이 각각 1명 있었다. 9. 전체 피부질환의 유무에 대하여, 근 We investigated the 1,004 workers who worked in a automobile factory to study the epidemiologic characterists of dermatoses due to cutting oils. Among the workers, 667(66.4%) answered the questionaire. They are belong to 5 departments of the factory-the Engine-Work(258 workers), Gasoline engine Assembly(210), Diesel engine Assembly(96), Power train Work(86), Power train Assembly(17). We measured the oil mist concentration in air of the departments and examined the workers who had dermatologic symptoms. The results were follows; 1) Oil mist concentration ; Of all measured points(52),9 points(17.2%) exeeded $5mg/m^3$- the time-weighed PEL-and one department had a upper confidence limit(95%) higher than $5mg/m^3$. 2) Dermatologists examined 213 workers. 172 of them complained any skin symptoms at that time - itching(32.5%), papule(21.6%), scale(15.7%), vesicle(12.5%) in order. The abnormal skin site found by dermatologist were palm(29.3%), finger & nail(24.6%), forearm(16.2%), back of hand(8.4%) in order. 3) As the result of physical examination, we found that 160 workers had skin diseases. Contact dermatitis was the most common; 69 workers had contact dermatitis alone(43.1%), 11 had contact dermatitis with acne(6.9%), 10 had contact dermatitis with folliculitis(6.3%), 1 had contact dermatitis with acne & folliculitis, and 1 had contact dermatitis with abnormal pigmentation. Others were folliculitis(9 workers, 5.6%), acne(8, 5.0%), folliculitis & acne (2, 1.2%), keratosis(1, 0.6%), abnormal pigmentation (1, 0.6%), and non-specific hand eczema (47, 29.3%). 4) The prevalence of any skin diseases was 34.0 pet 100 in cutting oil users, and 13.3 per 100 in non- users. Especially, the prevalence of contact dermatitis was 23.0 per 100 in cutting oil users and 23.0 per 100 in non-users. 5) We tried patch test(standard serise, oil serise, organic solvents) on 49 patients to differentiate allergic contact dermatitis from irritant contact dermatitis and found 20 were positive. 6) In a multivariate analysis(independant=age, tenure, kinds of cutting oil), the risk of skin diseases was higher in the water-based cutting oil user and both oil user than non-user or neat oil user(odds ratio were 2.16 and 2.78, respectively). And the risk of contact dermatitis was much higher at the same groups(odds ratio were 5.16 and 6.82, respectively).

후천성 반응성 천공성 교원증 3예

강병덕,양재석,김일환,송해준,오칠환 ( Byung Duk Kang,Jae Seok Yang,Ill Hwan Kim,Hae Jun Song,Chil Hwan Oh ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.1

Reactive perforating collagenosis(RPC) is a rare perforating disorder characterized by transepithelial elimination of altered collagen. Two types of RPC occur:inherited and acquired. The acquired type arises during adult life without a familial tendency. It is accompanied by scratching and usually is associated with diabetes mellitus, chronic renal failure, lymphoma and AIDS. We report three cases of the acquired form of RPC. Pruritus was the common factor among them. In two patients, chronic renal failure and hyperthyroidism were associated with RPC. Histopathological and ultrastructural findings showed transepithelial elimination of collagen. Control of itching was helpful for clearing the lesions.(Kor J Dermatol 1997;35(1):204∼208)

종축 흑색조 양상을 보인 조갑하 Bowen 병

오용섭(Yong Sub Oh),홍진호(Jin Ho Hong),계영철(Young Chul Kye),오칠환(Chil Hwan Oh) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.3

Bowens disease is an intraepithelial squamous cell carcinoma. It occur s anywhere on the surface of the body. Bowens disease involving the nail bed epithelium is very rate. Recently, some literatures showed that human papillomavirus is sssociated with squamous cell carcinoma of the finger by in situ hybridization or polymerase chain reaction. A 30-year-old housewife deve]oped longitudinal melanonychia with as imptomatic fissured nail plate on her right third finger. The hiat,ologic specimen showed diffuse anaplastic change confined to the nail bed epithelium. The subungual eyithelium was found to contain human papillomavirus DNA type 31/33/35 with the use of in situ hybridization. (Kor J Dermatol 1994; 32(3): 514-517)