압출성형과 효소처리가 신령버섯 β-Glucan의 추출에 미치는 영향

길선국(Sun-Kook Gil),신중엽(Joong-Yup Shin),강대일(Dae-Il Kang),류기형(Gi-Hyung Ryu) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3

압출성형공정과 효소처리가 신령버섯 추출물의 β-glucan 함량에 미치는 영향을 조사하였다. 스크루 회전속도 250 rpm, 원료 사입량 100 g/min, 사출구 3 mm 원형으로 고정하였으며, 수분 함량 20, 30%, 배럴 온도 130, 140°C로 조절하여 압출성형 신령버섯을 제조하였다. 수분 함량 20%, 배럴 온도 130°C의 압출성형공정을 통한 신령버섯 추출혼합물은 β-glucan 함량이 16.91 mg/g으로 다른 공정들보다 증가하였다. 수분 함량 20%, 배럴 온도 130°C의 압출성형공정과 Rohament CL로 처리한 추출물의 β-glucan 함량은 같은 압출성형공정의 Viscozyme L 처리 추출물 17.35 mg/g과 Plantase TL 처리 추출물 17.51 mg/g보다 18.32 mg/g으로 가장 많이 증가하였다. 결론적으로 압출성형공정과 Rohament CL 처리한 추출물의 β-glucan 함량이 18.32 mg/g으로 압출성형공정과 효소처리를 하지 않은 대조구의 14.45 mg/g보다 약 26.7% 정도 증가함을 나타내었다. 이는 신령버섯 추출 시에 압출성형공정과 효소처리가 β-glucan 함량을 대조구에 비해 증가시킬 수 있다고 판단되었다. This study analyzed changes in β-glucan content in Agaricus blazei Murill concentrates according to extrusion and extraction conditions. Screw speed and feed rate were fixed to 250 rpm, and 100 g/min, respectively. Moisture contents (20 and 30%) and barrel temperature (130 and 140°C) were adjusted. β-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of 130°C was higher compared to other extrusion conditions. β-Glucan content of the extruded sample at a moisture content of 20% and barrel temperature of 130°C treated with Rohament CL enzyme was higher compared to Viscozyme L, and Plantase TL enzyme treatments under the same extrusion conditions. In conclusion, extrusion and pretreatment with Rohament CL enzyme enhanced yield of β-glucan extract.

산자나무(Hippophae rhamnoides L.) 추출물, 오일 및 이들을 함유한 나노에멀젼의 주름개선 효능 평가

최선정(Seon-Jeong Choi),김나리(Na-Ri Kim),신중엽(Joong-Yup Shin),이관모(Kwan-Mo Lee),경기열(Kee-Yeol Kyong) 한국화장품미용학회 2016 한국화장품미용학회지 Vol.6 No.2

This study was conducted to investigate antioxidant activity of fruit and leaf extracts of seabuckthorn (Hippophae rhamnoides L) by different solvents and then further evaluated the anti-wrinkle efficacy and cytotoxicity in seabuckthorn extracts, seabuckthorn oil, and the seabuckthorn seed oil nanoemulsion that emulsified the mixture of seabuckthorn leaf extract and seed oil. In total polyphenol and flavonoid contents, the contents of ethanol extracts were higher than that of water extracts. Especially, 70% ethanol extracts was the highest contents. Also, the total polyphenol and flavonoid contents of leaf extract were significantly higher than that of fruit extract. The flavonol and carotenoid contents were similar pattern as well. The DPPH radical scavenging activity of leaf extract was significantly higher than that of fruit extract and also increased in accordance with solvent concentrations. Therefore 70% ethanol extractions of fruit and leaf were optimum condition. In cytotoxicity and matrix metalloproteinase-1 (MMP-1) inhibitory activity, the seabuckthorn leaf ethanol extract and seed oil were no cytotoxicity and significantly MMP-1 inhibitory activity. Nanoemulsion that emulsified seabuckthorn leaf ethanol extract and seed oil has anti-wrinkle efficacy. In conclusion seabuctkorn leaf extract, seabuckthorn seed oil, and seabuckthorn nanoemulsion could be a potential functional source of cosmetics.

신령버섯(Agaricus blazei Murill) 열수 추출물의 면역 활성에 미치는 영향

강인순(In Soon Kang),김랑이(Rang Ie Kim),김광섭(Gwang Sub Kim),김나리(Na Ri Kim),신중엽(Joong Yup Shin),김채균(Chaekyun Kim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.11

본 연구에서는 신령버섯의 면역기능 개선 효과를 마우스를 이용한 동물 모델에서 면역기관의 무게와 세포증식, 자연살해세포 활성, 사이토카인 분비능을 측정하고 대식세포 증식과 활성을 측정하여 평가하였다. BALB/c 마우스에 저(4 mg/kg), 중(20 mg/kg), 고(100 mg/kg) 농도의 신령버섯을 21일간 경구로 투여하였다. 마우스를 희생하여 체중 및 면역장기 무게, 비장세포의 증식과 사이토카인 생성, 자연살해세포의 활성을 측정하였다. 그 결과 신령버섯은 마우스의 체중, 간, 비장, 흉선의 무게에 영향을 주지 않았으며, 비장세포의 증식에 유의한 효과가 없었다. 또한 비장세포의 IL-4과 IL-12 생성을 억제하였으며, 마우스 자연살해세포의 활성을 현저하게 증가시켰다. 정상 마우스에서 분리한 비장세포에 신령버섯을 처리한 in vitro 실험에서는 신령버섯 5~100 μg/mL에서 농도 의존적으로 비장세포의 증식과 IFN-γ 생성을 증가시켰다. 신령버섯은 대식세포인 RAW 264.7 세포의 증식을 100 μg/mL 농도까지 농도 의존적으로 증가시켰으며, 대식세포에 의한 NO의 생성을 농도 의존적으로 증가시켰다. 이상의 결과를 종합해 보면 신령버섯을 마우스에 3주간 투여하면 동물의 체중, 면역장기의 무게와 면역세포의 증식에는 영향을 미치지 않지만 자연살해세포의 활성을 70% 가량 증가시키며 IL-4와 IL-12의 생성을 억제한다. 정상 마우스에서 분리한 비장세포에 신령버섯을 처리하면 세포증식과 IFN-γ 분비가 증가되고, 대식세포인 RAW 264.7 세포의 증식과 NO 생성이 증가된다. 따라서 신령버섯은 바이러스에 감염된 세포나 암세포를 죽이는 자연살해세포와 대식세포의 활성을 증가시켜 면역반응 조절에 중요한 역할을 할 것으로 기대된다. The edible mushroom Agaricus blazei Murill is known to have many physiological functions, including antitumor, antiviral, and anti-inflammatory effects. Aqueous extracts were obtained by extracting A. blazei in water at 90°C for 15 h, followed by spray-drying with dextran at a 70:30 ratio. In this study, we examined the immunomodulatory effect of A. blazei Murill water extract (ABM) in BALB/c mice. Mice were administered orally with 4, 20, and 100 mg/kg of ABM for 21 days. ABM-treated mice did not show significant differences in body and organ weights compare to saline-treated control mice. Splenocytes isolated from ABM-administered mice revealed similar levels of cellularity and proliferation compared to control mice, whereas they showed increased natural killer (NK) cell activity and decreased IL-4 and IL-12 production. Different from in vivo results, splenocytes isolated from normal mice showed increased proliferation and INF-γ production following ABM treatment in vitro. In addition, ABM treatment enhanced macrophage proliferation and nitric oxide (NO) production in a dose-dependent manner. However, ABM had no effect on LPS-induced NO production. These results suggest that A. blazei modulates immune function by increasing NK cell activity and macrophage function.

Tenax-GC를 利用한 大豆乳와 醱酵大豆乳의 風味成分 分析

愼重燁,李瑞九,金顯五,朴啓仁 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

Soy milk prepared from soy protein isolate and soy milk fermented with Lactobacillus acidophilus were adsorbed and desorbed by using Tenax-GC method and analyzed by Gas Chromatograph. It was found that this techingue was very efficient when the analytical results was expressed by the ratis of the peak area of each volatile to that of a fixed concentration of internal standard. Acetaldehyde, ethyl vinyl ketone, n-hexanal, n-hexanol, and 1 -octen-3-ol existed in soy milk. The content of acetaldehyde and diacetyl was increased while that of n-hexanal was reduced by lactic fermentation.

Lactobacilli의 Lysine Analog 내성 변이주에 의한 Lysine 생산

신중엽,박계인,이종대 慶熙大學校 1985 論文集 Vol.14 No.-

S-2-Aminoethyl L-Cysteine(ABC), and analong of L-lysine, significantly inhibited the growth of wild type strains of Lactobacillus acidophilus IFO 3205, Lactobacillus acidophilus IFO 13952, Lactobacillus bulgaricus ATCC11842, and Lactobacillus bulgaricus IFO 353g. The growth inhibition of these strains depended on AEC concentration. In presence of 10ug/ml of AEC concentration the growth of these strains was inhibited completely. AEC resistant mutants were induced by N-methyl-N-nitro-N-nitrosoguanidine(NTG) and Ethylmethane sulfonate(EMS) treatment. Almost all resistant mutants produced lysine. Lysine productivity of mutants had no insignificant relation to method of mutagen treatment. Also lysing productivity of mutants had nothing to do with concentration of AEC. Lysince productivity of lysine analog resistant mutants obtained from first mutation was increased by further mutation. Among the AEC resistant mutants, LaNA-412 was selected as lysine producing mutant. LaNA-412 produced 103 ug/ml L-lysine.

Lactobacilli의 Lysine Analog 내성 변이주에 의한 Lysine 생산

신중엽,박계인,이종대 경희대학교 부설 식량자원개발연구소 1985 硏究論文集 Vol.6 No.-

S-2-Aminoethyl L-Cysteine(ABC), an analog of L-lysine, significantly inhibited the growth of wild type strains of Lactobacillus acidophilus IF0 3205, Lactobacillus acidophilus IF0 13952, Lactobacillus bulgaricus ATCC 11842, and Lactobacillus bulgaricus IF0 353g. The growth inhibition of these strains depended on AEC concentration. In presence of l0ug/ml of AEC concentration the growth of these strains was inhibited completely. AEC resistant mutants were induced by N-methyl-N-nitro-N-nitrosoguanidine(NTG) and Ethylmethane sulfonate(EMS) treatment. Almost all resistant mutants produced lysine. Lysine productivity of mutants had no insignificant relation t o method of mutagen treatment. Also lysine productivity of mutants had nothing to do with concentration of AEC. Lysine productivity of lysine analog resistant mutants obtained from first mutation was increased by further mutation. Among the AEC resistant mutants, LaNA-412 was selected as lysine producing mutant. LaNA-4 12 produced 103μg/ml L-lysine.

무기염류의 첨가가 김치성분의 변화에 미치는 영향

박상현,신중엽,박계인 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

This study was to investigate the effects of addition of inorganic salts (KCl, MgCl_(2).6H_(2)O, CaCl_(2), 2H_(2)O) on the change of kimchi components. To this end, kimchi was prepared by adding inorganic salts and fermented at low (6-10℃) and high (20-22℃) temperatures. pH, acidity and pectic substances of kimchi were analyzed. During fermentation, pH, AIS (Alcohol insoluble solid), and HCl soluble pectin (HClSP) were decreased, while acidity and hot water soluble pectin (HWSP) were increased. These changes were more remarkable at high temperature than low temperature. The addition of inorganic salts in kimchi preparation influenced the changes of pH, acidity, HWSP, and HClSP at both low and high temperatures. The changes of pH and acidity were more remarkable in kimchi, prepared with addition of MgCl_(2) .6H_(2)O or CaCl_(2) .2H_(2)O than KCl. The changes of HWSP and HClSP were more marked in kimchi, which was added with KCl than MgCl_(2). 6H_(2)O or CaCl_(2) .2H_(2)O

乳酸菌 變異株의 誘導와 利用(第1報) : Nitrosoguanidine에 依한 乳酸菌 變異株의 誘導 Induction of Lactic Acid Bacteria Mutants by Nitrosoguanidine

朴啓仁,愼重燁 慶熙大學校 1986 論文集 Vol.15 No.-

Two strains of Lactobacillus acidophilus NRRL B-2178 and Lactobacillus plantarum ATCC 8014 were treated with N-methyl-N-nitro-N-nitrosoguanidine(NTG). In case of Lactobacillus acidophilus NRRL B-2178, 71 possible mutants were isolated. There was considerable variation among mutants in acid production and relative amounts of volatile compounds of soy milk prepared from soy protein isolate. LaHA-6 which was excellent in acid production and relative amounts of volatile compounds was found to possess stable characters after 20 daily transfer in MRS broth. In case of Lactobacillus plantarum ATCC 8014, 44 possible mutants were isolated. There was considerable variation among mutants in acid and CO_2 production of modified Elliker's broth medium and MD broth medium, respectively. LpLA-10 which produced lower amounts of acid and higher amounts of CO_2 was found to possess stable characters after 20 daily transfer in MRS broth.

乳酸菌 變異株의 誘導와 利用(第1報) : Nitrosoguanidine에 依한 乳酸菌 變異株의 誘導 Induction of Lactic Acid Bacteria Mutants by Nitrosoguanidine

朴啓仁,愼重燁 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

Two strains of Lactobacillus acidophilus NRRL B-2178 and Lactobacillus plantarum ATCC 8014 were treated with N-methyl-N-nitro-N-nitrosoguanidine(NTG). In case of Lactobacillus acidophilus NRRL B-2178, 71 possible mutants were isolated. There was considerable variation among mutants in acid production and relative amounts of volatile compounds of soy milk prepared from soy protein isolate. LaHA-6 which was excellent in acid production and relative amounts of volatile compounds was found to possess stable characters after 20 daily transfer in MRS broth. In case of Lactobacillus plantarum ATCC 8014, 44 possible mutants were isolated. There was considerable variation among mutants in acid and CO_(2) production of modified Eliiker's broth medium and MD broth medium, respectively. LpLA-10 which produced lower amounts of acid and higher amounts of CO_(2) was found to possess stable characters after 20 daily transfer in MRS broth.

韓國大豆食品의 Oligo糖 消長에 關한 硏究

朴啓仁,愼重燁,金鍾國 경희대학교 부설 식량자원개발연구소 1983 硏究論文集 Vol.4 No.-

Three Korean variety of soybeans and five ordinary fermented soybean pastes collected from 5 districts were studied for oligosaccharides. Oligosaccharides in soybean (Bong Eui Variety), meju, and fermented soybean pastes were determined. The changes of oligosaccharides during the meju preparation were investigated. And then oligosaccharides hydrolyzing micro-organism was isolated and identified. The results obtained are as follows: 1) All of the three Korean variety of soybeans were detected for sucrose, raffinose, and stachyose. In case of five ordinary fermented soybean pastes, three samples collected from Seoul, Busan, and Daegu were detected for sucrose, the only one sample collected from Busan was detected for traces of raffinose and stachyose, and the rest was not detected for these oligosaccharides. 2) Bong Eui variety of soybean contained 42.3㎎/g of sucrose, 12.5㎎/g of raffinese, and 32.5㎎/g of stachyose. Meju contained 2.48㎎/g of sucrose, 0.01㎎/g of raffinose, and 0.03㎎/g of stachyose. And then fermented soybean paste contained 0.25㎎/g of sucrose. 3) As the result of steeping and cooking the soybean, the contents of sucrose, raffinose, and stachyose in soybean decreased to 66.9%, 72%, and 69.5% respectively. 4) Oligosaccharides in cooked soybean decreased a little at early stage of fermentation and almost disappeared except sucrose in 40 days. 5) Oligosaccharides hydrolyzing bacteria, strain B-12 was isolated from meju and identified as Bacillus sp.