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분자 분광학적 방법에 의한 소 심장 Proteolipid 의 구조적 연구
경기열,채쾌 ( Kee Yeol Kyong,Quae Chae ) 생화학분자생물학회 1999 BMB Reports Vol.19 No.2
UV-VIS difference and fluorescence spectroscopic techniques were employed to probe the structural changes of the membrane proteolipid with the different solvent systems. The purified proteolipid from bovine heart was dissolved in two different solvent systems (water, organic solvent: chloroform-methanol) and various optical spectra with these solvent systems were measured. The number of perturbed tryptophan residues were estimated to be 1.8 in water and 1.2 in organic solvent, respectively. In addition, the higher fluorescence quenching effects on tryptophan residues and/or ANS by acrylamide were observed in water than the one in organic solvent system. The two results strongly support the hydrophobic interaction needed for the oligomerization of the proteolipid in water environment. However, a certain elucidation for the oligomerization state of this protein can not be provided at the moment.
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경기열 ( Kee-yeol Kyong ),이천구 ( Cheon-koo Lee ) 대한화장품학회 2006 대한화장품학회지 Vol.32 No.4
Emulsion is a dispersion system among liquids which are not miscible together. There are numerous cosmetic raw materials which have different physicochemical properties. Therefore, emulsion technology is very useful in cosmetics. With the development of emulsifier, several emulsification technologies have been developed. Since HLB method by Griffin in 1950's, PIT method, gel method, and D-phase methods, etc, have been developed. Recently, the application of natural emulsifier and polymeric emulsifier increases in cosmetics in order to achieve enhanced safety and biocompatibility. Besides nano-emulsion, multiple-emulsion, liquid crystal emulsion, and Pickering emulsion have been developed and applied as means of differentiating appearance and texture of products and achieving enhanced delivery of active ingredients. Meanwhile, the application studies of nano-dispersed structural system such as liposome or cubosome are on progress. Liposome is a bi- or multi-lamella layer dispersion system composed of amhiphilic molecules - phospholipids which are main components of plasma membrane. Cubosome also is a nano-sized dispersion system composed of a specific molecule like glyceryl monoloeate derived from natural products. And it has a cubic bicontinuous structure in water due to its unique molecular structure. Incorporating compounds (active materials) into such nano-particles can increase biocompatibility and delivery efficiency of target compounds. Manufacturing process and application of cosmetic emulsions and nano-particles are briefly introduced in this paper.
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Structural Study on Bovine Heart Proteolipid by the Optical Spectroscopic Methods
경기열,채쾌,Kyong, Kee-Yeol,Chae, Quae 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
UV-VIS difference and fluorescence spectroscopic techniques were employed to probe the structural changes of the membrane proteolipid with the different solvent systems. The purified proteolipid from bovine heart was dissolved in two different solvent systems (water, organic solvent: chloroform-methanol) and various optical spectra with these solvent systems were measured. The number of perturbed tryptophan residues were estimated to be 1.8 in water and 1.2 in organic solvent, respectively. In addition, the higher fluorescence quenching effects on tryptophan residues and/or ANS by acrylamide were observed in water than the one in organic solvent system. The two results strongly support the hydrophobic interaction needed for the oligomerization of the proteolipid in water environment. However, a certain elucidation for the oligomerization state of this protein can not be provided at the moment. UV-VIS difference 분광법과 형광 분광법을 이용하여 용매 변화에 따른 세포막 proteolipid의 구조적 차이를 알아보았다. 소의 심장으로부터 분리 정제된 proteolipid을 두 개의 다른 용매계(물, 유기용매 chloroform-methanol)에 용해시키고 여러가지 광 스펙트라를 측정하였다. 단백질의 바깥쪽으로 노출된 트립토판 아미노산 잔기의 수는 물 속에서 1.8개인 반면 유기용매 속에서는 1.2개로 나타났다. 부가하여, 아크릴아미드에 의한 트립토판 잔기와 ANS의 형광에 대한 quenching 효과도 유기용매 속에서보다 물 속에서 더 크게 나타났다. 이상의 결과들은 이 세포막 proteolipid가 oligomerization 시 요구되는 소수성 상호작용에 대한 이론을 강하게 뒷받침해 주고 있다. 그러나 현 상태에서 이 단백질의 이온 수송 현상을 설명할 수 있는 정확한 oligomerization state를 밝히기는 어려운 실정이다.
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기능성 소재 개발용 발효 균주 탐색을 위한 민물에서 분리한 호염성 미생물의 효소 생산능 분석
경기열 ( Kee Yeol Kyong ) 강원대학교 농업생명과학연구원 2021 농업생명환경연구 Vol.33 No.2
This study characterized halophilic microorganisms isolated from freshwater in Korea. Samples were cultivated on marine agar at 37°C for several days. A total of 24 pure colonies obtained from single colony isolates were used for 16S rRNA-based phylogenetic analysis by an ID service located in EzBioCloud database. The obtained 16S rDNA sequences allocated the isolated strains were divided into 3 phyla, 11 families, 12 genera, and 17 species. To confirm whether the isolated strains were candidates for the fermentation of diverse food ingredients and development of functional cosmetic ingredients, amylase, lipase, and protease enzyme assays were performed individually; the tests revealed that 17 strains possessed at least one enzyme activity. In particular, 11 strains exhibited activities of 2 enzymes and 3 strains produced auxin. These strains might be useful in the food and cosmetic industries. Therefore, the study findings will contribute to securing domestic biological resources and improving hydrolytic enzyme activities using isolated strains.
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고분자 수용액의 레올러지 특성이 캡슐의 침강에 미치는 영향
김동주 ( Dong-joo Kim ),김정아 ( Jung-ah Kim ),경기열 ( Kee-yeol Kyong ),윤명석 ( Moung-suk Yoon ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.1
An aim of this study is to study the correlation between rheological properties and sedimentation of capsules in aqueous polymer solution with low viscosity. Rheological properties of aqueous polymer solutions were controlled by carbomer (C), acylate/C10-30 alkyl acylate crosspolymer (AC), and ammonium acryloyldimethyltaurate/VP copolymer (AV). Small amount of polymer C solution had the highest viscosity and yield stress of polymer AV solution was higher than that of polymer C solution in the same viscosity when the concentration of polymer AV exceeded 0.35 wt%. Each aqueous polymer solution was tested and the results showed that as viscosity and yield stress increased, the sedimentation ratio of capsules decreased. The viscoelasticity data also showed the same tendency in a shear stress range of 0.1 to 2.0 Pa. These results demonstrated that the rheological properties of polymer solutions had a strong correlation with the sedimentation of capsules. When polymer I and AV were used, there was a synergistic effect and the correlation between rheological properties and sedimentation of capsules was very complicated. It was assumed that the characteristics of polymer structure and interaction between polymers caused this phenomena.
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유화제로서 실리카를 이용한 유중수형 에멀젼의 안정성에 미치는 수상부 조성의 영향
김진황 ( Jin-hwang Kim ),김송이 ( Song-e Kim ),경기열 ( Kee-yeol Kyong ),이은주 ( Eun-joo Lee ),윤명석 ( Moung-seok Yoon ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3
The extent of silica flocculation can be modified by varying the silica concentration, aqueous phase pH, salt and polvmer concentration. High volume fraction W/O emulsion stabilized by hydrophobic silica was established with various aqueous phase conditions for cosmetic application. By increasing the silica concentration up to 1.0 wt%, the size of droplet decreased. A high silica concentration increased the viscosity of continuous oil phase by network formation, which resulted in target size of droplet. The stability of W/O emulsion is improved as increasing the aqueous phase pH. At low and intermediate pH, the emulsions became more stable by adding salt (0.083 mol dm<sup>-3</sup> MgSO<sub>4</sub>). At high PH, the presence of salt caused significant destabilization. The gelation behavior of the emulsion indicates that the effect of salt on silica-stabilized emulsion is derived from an electrostatic attraction. The addition of xanthan gum in aqueous phase increased the mono-dispersity of the W/O emulsion by making water more hydrophobic and hindering the recombination of droplets. In conclusion, these results indicate that very stable emulsifier-free, finely dispersed W/O emulsion can be achieved for cosmetic application by changing the aqueous phase composition.
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