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      • 陣영湯加減方의 抗腫瘍效果에 關한 實驗的 硏究

        丁俊宅,文九,文錫哉 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1

        科學技術의 發展에 따른 生活 및 醫療 수준의 向上에도 불구하고 産業發展에 따른 環境汚染, 스트레스 增加등 그 逆機能 때문에 發癌物質 또는 刺戟에의 露出등으로 인해서 全世界的으로 癌 으로 因한 死亡率이 增加되고 있다. 癌은 現在 人類의 健康을 위협하는 主要한 疾患의 하나로, 오늘날 우리 나라에서 疾患으로 因한 死亡原因中 首位를 차지하고 있다. 이러한 癌의 發生은 80%-90%가 環境的 因子에 起因한다고 報告되고 있는데 앞으로 環境性 發癌物質이 점차 增加됨에 따라 疾患者의 發生도 더욱 增加될 것으로 豫想된다. 癌의 西醫學的 治療는 주로 手術療法, 放射線療法, 化學療法 및 免疫療法이 活用되고 있다. 이중 手術療法과 放射線療法은 癌이 全身에 轉移, 增殖되기 전에 實施함으로써 效果를 거둘 수는 있으나, 癌이 原發病巢 以外에까지 轉移, 增殖되었을 경우에는 이 두가지 方法의 局所療法만으로서는 좋은 治療效果를 거주기 어렵다. 따라서 최근 抗癌劑에 의한 化學療法이 다른 方法보다 有效하다고 認定이 되어 가장 많이 活用되고 있으나, 癌腫에 대한 感受性差異, 治療後의 副作用, 再發 및 合倂症 등의 問題點 때문에 副作用을 減少시키면서 抗癌效果를 높이는 治療法이 계속 硏究되고 있다. 이에 1972年 美國 國立癌硏究所에서 實施한 抗癌劑 開發硏究에 있어서 抗腫瘍性 Screening test에 利用된 物質中 약 47%가 天然物質이었다는 사실은 注目할만 하다. 韓醫學的 治療法은 最近에 辨證施治를 根幹으로 하여 健脾益氣를 爲主로한 扶正祛邪法과 扶正培體法이 많이 活用되고 있으며 扶正法으로는 益氣健脾, 滋陰補血, 溫補脾?? 등이, 祛邪法으로는 活血祛療法, 淸熱解毒法, 軟堅化痰法 등이 活用되고 있는데, 이를 根據로 하여 韓藥의 複方,單味,單味抽出物 등의 投與와 鍼,灸,水鍼,電鍼, laser鍼등의 治療方法을 手術 後 或은 化學療法이나 放射線治療와 竝行하여 治療效果가 向上되었다고 報告하고 있다. 抗癌劑의 抗癌效果 程度를 檢査하는 方法은 크게 5가지로 分類되는데, 하나는 細胞를 藥劑에 露出시킨 후 顯微鏡을 통하여 나타난 形態學的 變化를 가지고 藥劑의 效果程度를 決定하는 方法이고, 둘째는 Dye exclusion assay 方法으로 살아있는 細胞가 trypan blue, eosin, nigrosin과 같은 染色液에 染色되지 않는 점을 利用한 것이며, 세 번째는 抗癌劑 대자석. 丁香, 枇杷葉, 抗癌作用을 가진 龍癸, 半技蓮, 牡蠣, 夏枯草, 猪? 等의 藥物로 구성되어 있다. 近來 單一藥物의 抗癌效果에 대한 實驗的 硏究로는 金^27,28)등에 의한 人蔘, 鹿茸 의 抗體生産 抑制 緩和效果 및 任^29)에 의한 魚腥草,鹿血, ??, 穿山甲 등이 正常 免役細胞에는 거의 毒作用을 일으키지 않으면서 强力한 抗癌效果가 있다는 報告, 金^31-34)등에 의한 紫率, 東風蔡, 靈芝, 仙鶴草, 巴豆 등이 抗癌作用 및 免疫反應에 效果를 미친다는 報告 등이 있고, 複合藥劑 藥物의 抗癌效果에 대한 硏究로는 姜^36-38)등에 息賁丸, 肥氣丸 및 비기환이 白血病과 淋巴腫患者에서 抽出한 癌細胞에 抗癌效果가 있다는 報告 및 金^37-39)등에 의한 伏梁丸, 비 氣丸 및 消積正元散이 各種 癌細胞柱의 成長沮碍 效果, 尹^30,35,40,42)등에 의한 六君子湯, 小柴胡湯, 四妙湯, 大柴胡湯, 防毒湯, 半夏白 朮天麻湯, 巴豆를 加味한 四君子湯 및 四物湯 등이 抗癌作用 및 免疫反應에 效果를 보인다는 報告등이 있었으나, 아직까지 진령탕가감방의 抗癌效果에 대한 實驗的硏究는 없었다. 이에 著者는 抗癌效果가 있을 것으로 推定되는 진령탕가감방의 抗腫瘍效果를 알아보기 위하여, 水溶性 抽出液을 얻은 후 現代 病理學的으로 多用하는 colony形成制實驗, SRB assay에 의한 抗腫癌效果, 生命延長率, 化學的인 方法과 癌腫細胞 移植에 의한 腫瘍 誘發을 통하여 癌 發生 및 그 經過에 미치는 影響 및 NK細胞活性에 미치는 影響 등을 觀察하였다. 本 實驗에서는 진령탕가함방의 腫瘍細胞에 대한 增殖抑制作用을 觀察하고자 in vitro test인 colony形成抑制實驗과 SRB assay를 利用하였는데, 진령탕가함방의 抽出液이 腫瘍細胞의 成長을 抑制하는 效果가 濃度依存的으로 增加하는 結果를 보여 抗腫瘍의 效果가 있음을 나타냈고(table3,4)in vivo assay로서 sarcoma 180 복수암세포를 移植한 마우스의 平均生存期間 및 生命延長率이 뚜렷하게 增加되었다.(table5). 또한 진령탕가함방 抽出液 마우스에 투여하면 MCA 유도한 종양 및 3LL 細胞移植에 의한 腫瘍의 發生率 및 發生된 腫瘍의 크기가 현저히 減少되었다.(table7). 이러한 效果는 진령탕가감방이 感受性 癌腫細胞에 직접적으로 影響을 미쳐 그 增殖이 抑制되었을 可能性을 생각할 수 있다. 그렇지만 本 硏究에서는 癌腫特異性에 관한 結論을 내릴 수는 없으므로 癌腫特異性 관한 硏究는 進行해야 할 것으로 생각된다. 또 다른 可能한 진영탕가함방 抽出液 抗腫瘍 作用機轉은 진영탕가함방 抽出液이 NK細胞의 活性을 亢進시킨 實驗結果로 類推하여, 진영탕가함방 抽出液이 腫瘍마우스에 非特異的인 免疫活性을 促進시킴으로써 진영탕가함방 抽出液의 抗腫瘍作俑과 발현괼 可能性도 생각할 수 있다. 따라서 앞으로 여러 癌腫細胞에 대한 진영탕가감방 抽出液의 抗 腫瘍 作用과 腫瘍動作에서의 진영탕가함방 抽出液 投與에 의한 生體生理現象의 變化, 生理活性物質의 本態 등을 밝히기 위한 보다 具體的이고 廣範圍한 硏究가 數行되어져야 되리라고 思料된다. 細胞性 免疫反應中 重要한 役割을 하는 작동세포증 NK細胞는 여러 腫瘍에 대해 自然殺害能을 보여 소위 T非依存性 免疫監視機轉에 관하여는 바이러스 및 細菌에 感染된 細胞를 破壞하는 活性을 가지며, IL-2 및Interferon 등에 의하여 活性이 增加된다. NK細胞가 표적세포를 破壞하는 機轉에 관해서는 아직 확실히 알려져 있지 않으나, 첫째로 표적세포를 認識하는 時期, 둘째로 融解機轉을 活性化시키는 時期, 세째로 NK lymphotoxin이 邊離되는 時期, 네째로 표적세포의 死成期로 나눌 수 있다. 本 實驗에서 진영탕가감방 抽出液으로 前妻理시 NK細胞의 표적세포와의 結合能은 물론 結合된 표적세포의 融海能이 促進된 結果를 보이는데(Table 8), 이와 같은 結果는 진영탕가감방 抽出液이 NK細胞의 표적세포 파괴 4단계 전체를 亢進시킨 結果로 認定된다. 이상에서 다음과 같은 결론을 얻었다. 1. 진영탕가감방 抽出液을 폐암세포주인 A549와 간암세포주인 hep3B 및 신장암 유래의 종양세포주인 Caki-l cell에 投與한 결과, in vitro test인 coloy形成抑制實驗과 SRB assay에서 모두 濃度依存的으로 암세포주 成長을 抑制하였다. 2. Sarcoma 180細胞를 마우스에 移植하여 平均生存期間 및 生命延長率을 測定하는 in vivo test에서는 진영탕가감방 抽出液이 마우스의 平均生存期間 및 生命延長率을 增加시키는 結果를 보였다. 3. 진영탕가감방 抽出液은 MCA로 誘導한 腫瘍의 發生率 및 發生腫瘍의 크기를 현저히 減少시켰으며. 3LL細胞 移植에 의한 腫瘍發生 또한 현저히 減少시켰다. 4. 진영탕가감방 抽出液은 NK細胞의 活性度를 增加시켰다. 특히 10ug/ml농도 에서 NK細胞 活性度가 가장 현저히 增加되었다. 以上의 結果로 보아 진영탕가감방은 癌腫細胞에 대한 直接的인 成長抑制 및 細胞의 活性을 통한 免疫增進에 의해 多樣한 抗腫瘍效果를 보인 것으로 思料된다. The sprig of Jinryungtang Gagambang(陳영湯加減方) has been used for curing as a traditional medicine without any experimental evidence to support the rational basis for their clinical use. This experiment was carried out evaluate the possible therapeutic or antitumoral effect of Jinryungtang Gagambang extract against vancer, and to study some mechanisms responsible for its effect. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, hep3B, Caki-1,Sarcoma 180 after exposure to Jinryungtang Gagambang extract using in ILS. colony forming efficiency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this were as folows. 1. As a result of exposure to Jinryungtang Gagambang extract, the proliferation of A549, hep3B, Caki-1, good correlation were shown from the results of SRB assay and those of clogenetic assay. 2. The administration of Jinryungtang Gagambang extract showed significant effects of increase of MST(mean survival time) and ILS(increased lufe span) depending on the increasing concentration. 3. Against squamous cell carcinoma induced by MCA, Jinryungtang Gagambang decreased not only the frequency of tumor production but also the number and weight of tumors per tumor bearing mice(TBM). Jinryungtang Gagambang also significantly supressed the development of 3LL cell-implanted tumors by frequency and their size, and some developed tumors were regressed by the continuous treatment of Jinryungtang Gagambang extract into TBM. 4. Jinryungtang Gagambang extract also increased NK cell activities. According to the above results, it could be suggested that Jinryungtang Gagambang extract has prominent antitumor effect.

      • 人蔘百合湯이 B16세포에 대한 세포독성능 및 C57BL/6계 생쥐의 폐전이암의 억제에 미치는 영향

        黃鎬俊,河智容 대한한방종양학회 1997 대한한방종양학회지 Vol.3 No.1

        종양은 그 발생원인과 성장기전이 자세히 밝혀져 있지 않는 질병으로 최근 사망원인의 제1원인으로 급격히 발생빈도가 증가하고 있다. 종양환자의 수는 점점 증가하는 추세이며, 그에 따른 사망자의 수도 늘고 있다. 종양을 치료하는 방법으로 수술요법·면역요법·방사선요법 ·화학약물요법·골수이식·호르몬요법등이 사용되어 왔으며, 최근에는 전통적인 한약재를 이용한 종양파괴 및 종양의 성장을 억제하는 약재의 연구가 활발히 시도되고 있다. 본 연구에서는 '노수토홍(노수토홍)'에 사용되는 인삼백합탕(人蔘百合湯)을 사용하여 종양파괴 및 종양의 성장억제능을 연구하고자 하였다. 연구방법 인삼백합탕을 전이암 실험에 다용(多用)되는 B16세포를 대상으로 세포독성을 MTT검사법에 의하여 실험한 후 IC_50을 측정하였다 그 후 동물실험으로 C57BL/6에 Bl6세포를 주입시킨 후 고형암의 중량과 폐에 전이된 흑색종의 집락의 수를 측정하였고, 생존기간의 연장정도를 측정하였다. 연구결과 인삼백합탕 구성약물의 시험관내 세포독성을 측정하기 위하여 MTT검사법으로 측정한 결과 농도에 비례하여 생존율은 감소하였고, IC_50을 산출한 결과 백출, 홍화, 계피, 인삼등이 낮은 수치를 나타내었다. 인삼백합탕엑스의 시험관내 세포동성은 농도에 비례하여 생존율이 감소하였고, IC_50은 0.0002437㎍/㎖ 을 나타내었다. Bl6세포를 C57BL/6의 복강에 주입시켜 고형종양의 무게를 측정한 결과 대조군에 비하여 유의성있는 감소를 보였다(P<0.05). Bl6세포를 C57BL/6의 꼬리정맥에 주입하여 폐전이암을 유발시킨 후 폐의 표면에 생긴 집락의 수를 측정한 결과 대조군에 비하여 유의성 있는 감소를 보였다(P<0.001). Bl6세포를 C57BL/6의 꼬리정맥에 주입하여 폐전이암을 유발시킨 후 생존일수를 측정한 결과 평균치가 대조군은 23일, 투여군은 26일을 나타내어 1l3%의 증가를 보였다- Oriental medicine as a candidate for effective cancer treatment recently gain positive concerns in fields of therapeutic oncology, that is why some herbal medicines have been empirically safer in toxicity than anticancer drugs used in western medicine. and to show excellent therapeutic efficacy in human trial. Thus. these effects by clinically applied-herbs have not yet fully demonstrated in experimental tumor model, This study was initiated to evaluate the antitumor effect of Insambaekhaptang as candidate of antitumor-herbal agent against Bl6 melanoma metastasized into C57BL/6 mice lung. In experiment to test whether Insambaekhaptang can directly kill cancer cells in vitro or not, Insambaekhaptang showed direct killing action in concentration or higher against B16 melanoma cells using MTT assay, and showed lower IC50. Another experiment to know whether Insambaekhaptang can inhibit growth and metastasis of cancer cell or not, Insambaekhaptang significantly inhibited Solid tumor by intraperiperal injected-melanoma and lung metastasis induced by intravenous injected-melanoma in inbred C57BL/6 mice. When quantitative survival time increasing, we could obtain results that increased 113% in treated by Insambaekhaptang. These results show that Insambaekhaptang can inhibit growth of B16 melanoma cells through various biological mechanisms,

      • 韓藥을 利用한 抗癌 實驗硏究의 傾向에 關한 考察

        金賢兒,林成祐,李源哲 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1

        Because there were lots of side effects and tolerances to the existing anticancer therapeutics, the experiment extraction the anticancer effct from medicinal herbs is in progress lively. Therefore the purpose of this study were to research the tendency and the course of anticancer studies. To research the tendency of anticancer studies, medicinal herbs of fifty three experimental papers were analyzed and to examine the coures of studies, anticancer papers in the medical world were used. The obtained results were as follows Methods of herbal medicinal treatments were elirmination the pathogenic factor(祛邪 ) and supporting healthy energy(扶正) method used. In this study. immediately tumor bearing and immune response were the most important point. The subject of immediately tumor bearing was not in the specific cancer but in the influence on the life span of general cancerous cells. In the experimental study of immume response, the effect on NK cell activity of medicinal herbs most studied. The combined usage of medicinal herbs and anticaner agent mostly intended to know whether it inhibits the tumor cell growth. The serum test and blood cell nunber test show if medicinal herbs inhibit side effect of anticancer agcnt. More than 80 percents of used medicinal herbs, there were anticancer activities. However anticancer experimental studies using medicinal herbs have two weak points. The one, it was difficult to choose a prescription according to differentiation of symptoms and signs(辨證論) of the Oriental Medicine, because we put to the test not a man but a mouse. The other, as we obseved the indirect effect of the whole physiological regulation caused by synergic effects of the compex prescription, we don't undersstand the detailed mechanism of the herb. Therefore, if the anticancer effect of the herb is proved the experiment, we should research the concrete medical action of medicinal herbs and immunological analysis of herbal medicines to the body.

      • 肺癌에서 轉移된 腦腫瘍患者의 漢方 治驗 1例

        李源哲,申洸湜 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        We have experienced a case of metastatic brain tumor originated from lung cancer treated by oriental mdcine (Herbd medication, Acupuncture therapy, Moxa therapy) and We have a good result from that case to report it. According to the thermaltic effects, it could be suggeed that Younggyaechulgamtanggagambang extracts and oriental medical symptomatic treatment were significant in improvement of the patient.

      • 행인 과루인 추출물이 마우스 대식세포주인 RAW264.7 세포주의 iNOS 발현 및 Superoxide 형성에 미치는 영향

        박정운,문석재,문구,원진희 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        Macrophage play a major role in host defence against infection and tumor development and this activity is regulated through the production of several mediators. In particular, the production of N0 by macrophages mediates killing or growth inhibition of tumor cells, bacteria, fungi and parasites. However, over-expression of iNOS by various stimuli, resulting in over-production of NO. contributes to the pathogenesis of septic shock and some infmammator and auto-immune disease. Thereforc, it would be valuable to develop potent and selective inhibitors of for potential therapeutic use. Thus the agent that supprees the expression of iNOS mRNA or enzyme protein will be usefull for the prevention of various diseases. We are intersted in identifying selective inhibitiors of iN0S which might be useful intreating inlammatory human diseases. In summary, we have demantrated that scopolein, isolated from Seman Armenicae and Radix Trichosalmis the production of NO induced by IFNy plus LPS in RAW 264.7 macrophages, The mechanism for the inhibition of N0 Production was due to suppression of the expression of iN0S mRNA or enzyme protein.

      • 藥酒를 이용한 抗癌治療에 관한 文獻的 考察

        鄭熙,姜在春 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        Anti-cancer medi-alcohols in onetnal herb prescriptions were divided by chef effects. Each medi- alcohols were analyzed and studied abut frequency, character, target, effect. Also, they were divided into the method of making medi-alcohols. The results were summerized as follows. 1. ne department of using mled-alcohol in anticancer herbval scriptions were limited in gynecology, dermatology and digcgive intemd medicine. There were a lot in gynecology. 2. In analysis about medid-alcohols of dermato scriptions by chef effect, the herbs of relieving exterior syndrome were a lot first and the herbs of invigorating-qi were second. 3. In analysis about meld-alcohols of dermato scripions by chef effect, the herbs of promoting blod circulation to remove blood stasis were a lot first and the herbs of invorating-qi were second. 4. The method of making medi-alcohol was divided into four types ; boiling, making up pills, ex-anon, filnation. I hope that the medi-alcohol will be used as prational references.

      • Melanoma B-16 에서 IL-1β 유발 GnT-Ⅲ,ⅴ 활성에 대한 瓜蔞仁 , 黃芩 추출물의 影響

        徐雲敎,鄭智天 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        The activity of N-acetylglucosamitnyltransferase(GnT) Ⅲ and V on a Melanoma B-16 was examined after incubation with interleukin 1 (IL-l ). While augumatting cell proliferation, IL-1 resulted in a decrease of GnT-Ⅲ activity and an increase of GnT-V activities. Consist-u with this, Mdamnu B-16 cultured with IL-l showcd increased affinlity to Daturam stramonium latin, which raogfuzes asialo-tri- and asialeo-tetra-antenery N-linked oligosaccharides. These results indicate that IL-1 modulate glycosyloaderse activity and the oligosaccharide sVtuture of target cells. On the omer hand, to investigate whether or not TKM-SG affect GnT-V gene expression in lung metastatic carcinoma, we used RT-PCR methods. TKM-SG treated cell lines showed low leve1s of secretion of GnT-V mRNA transcription·as elucidated by RT-PCR. Thus, with together lower GnT-V acnuty levels in the medium, TKM-SG was highly enative for lung cancer metastasis treatment and it was concluded that the medicine can be used as a potent anti-lung cancer metastasis medidcine.

      • 消積白朮散이 Bleomycin의 副作用減少와 抗癌效果에 미치는 影響

        金泰運,孫彰奎,趙鍾寬 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        l. Sojeokbaekchoolsan compound treatment decreased pulmonary fibrosis induced by Bleomycin. 2. At 1 and 2days after bleomycin ueament, Sojeokbaekchoolsan compund treatment decreased the number of neutrophils in bronchoalveolar lavage than those of bleomycin alone treatment. 3. SojeokMacoolsan Compound treamern increased the Fc receptor mediated rosette activity of alveolar macrophage decresed by bleomycin treatment. 4, At 1O days after bleomycin treatment,Sojeokbaekchoolsan compound treatment decreased the lipid peroxidation of tissue than those of bleomycin done treatment. 5. Anti-tumor activity of Sojeokbaekchoolsan and bleomycin compound group was higher than those of bleomycin alone treatd goto to asdic tumor caused by Sacoma-l8O tumor cells.

      • 血府逐瘀湯이 癌轉移 抑制에 미치는 影響

        李眞華,沈範相,安圭錫,崔昇勳 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

        To examine the effect of XuehdRUIng on the metastasis of cancer, the following experiments were carried out. Before the main experimnts, the cytotoxicity was measured by putting Xuefuzhuyutant sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. Weste blotting was carried out to examine the changes of Fos, Jun, Ets, Erk, md JNK. In vitro invasion assay with transwells coated by collagen and mangel was carried out. From the above reams the following conclusions were obtained. 1. The eXperimental result about cytotoxicity of Xuefuzhuyutang agaitst HTlO8O was a below. The stained cell count after beingtreated by by Xuefuzhuyutang sample 4M㎍/㎖ for 24 hours was 0.9% of total cells, and the stained cell count by Xuefuzhuyutang sample 100㎍/㎖ was 1.5% of total cells. Both were near the level of control group which showed O.6% stained. 2. Thee result of collagenase assay was as below. In Xuefuzhuyutang sample 400㎍/㎖, MMP2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disapappeared. In Xuefuzhuyutang samle 800㎍/㎖ both bands of MMP-2 and MMP-9 diSamared. 3. The results of westem blots for Jun, Fos, Ets, Erk, JNK were a below. In Xuefuzhuyutang sample 200㎍/㎖, Ets was reduced, and Jun, Fos were increased. 4 The result of invasion assay was as below. The number of cells which migrated gross trayswell membrane in Xuefuzhuyutang-treated group was less than that of control(+TPA) group. From the above results, it was concluded that Xuefuzhuyutang might inhibit the activity of collagenase not by the MMP-2, MMP-9 promoter but by the Other way.

      • 加味香砂六君子湯이 생쥐 小腸에서 放射線照射 後 保護效果와 Apoptosis에 미치는 影響

        이태업,김진성,윤상협,류봉하,박동원,류기원 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

        The present study was performed in order to evaluate the effects of the gamihyangsayukgunjatang on radioprotection and apoptoss in small intestines of mice after whole bdy irradiation. Two hundred forty mice were divided into 40 groups according to the radiation dose and the gamihyangsayukgunjatang treatment. The extracts of the herbal medicines were orally administered to each group differently before and/or after irradiation. The gamihyangsayukgunjatang treated groups were divided into 3 groups. Sample Ⅰ was the group treated with the gamihyangsayukgunjatang for 3 days before the radiation, sample Ⅱ was the group treated with the gamihyangsayukgunjatang for 3 days after the radiation. Sample Ⅲ was the group treated with the gamihyangsayukgunjatang for both 3 days before and after the radiation. To analyze the crypt survival, the micradony survival assay was used according to the Withers and Elind's method. To analyze the apptosis, the TUNEL assay was done. The reslts obtained are a follows : 1. From the microcolony survival assay, the gamihyangsayukgunjatang treated groups showed theradioprotective effect with a statistical ignificance(p<0.05), as compared to the control group. Comparing the radioprotective effect among the 3 groups, sample Ⅲ was statistically more significant than sample Ⅰand Ⅱ (p<O.O5). Sample Ⅰ showed no effect. In accordance with the research mentioned above, it is suggested that the radioprotective effect of the gamihyangsayukgunjatang is more useful for the treatment of the radiation injury rather that the prevention. 2. The results of the TUNEL assay showed that the apoptotic index in the gamihyangsayukgunjatang treaed group was slightly decreased with no effectiveness, as compared to the control group. According to the above results, it could be suggested that the gammyangsayukgummang has a prominent protective effect in mice intestines against the radiation damage. However, the radioprotective effect does not seem to be related to inhibition of the apoptosis.

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