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넙치 전 암컷 집단의 생산을 위한 연구 II. 성전환된 자성발생성 2배체 수컷의 자손 검정
방인철,김경길,김윤,김종현,Bang In-Chul,Kim Kyung-Kil,Kim Yoon,Kim Jong-Hyun 한국양식학회 1996 韓國養殖學會誌 Vol.9 No.2
본 연구는 성전환된 자성발생성 2배체 수컷의 정액을 보통 암컷 및 자성발생성 2배체 암컷의 성숙란에 인공수정시킨 후 자손의 성비를 분석하였다. 자성발생성 수컷의 유도는 자성발생성 2배체를 전장 13.5 mm에서 57.6 mm까지 62일간 $17{\alpha}$-methyltestosterone 10 ppb의 농도로 침지 처리하여 유도하였다. 자손 검정 결과, 보통 암컷과 자성발생성 수컷 교배군의 암컷 유도율은 평균 $37.4\%$로 낮게 나타났다. 그러나 자성발생성 2배체 암컷과 자성발생성 수컷 교배군에서는 8개 실험군 중 6개 실험군에서 $100\%$ 암컷이 유도 되었고 2개 실험군에서는 $96.9\%$ 및 $95\%$ 암컷이 유도되어, 전체 $99\%$ 의 암컷 유도율을 나타내었다. 이러한 결과는 자성발생성 2배체 암컷과 성전환된 자성발생성 2배체 수컷이 정상적인 생식 기능을 통해 교배되어 전 암컷 집단을 생산할 수 있음을 시사한다. A study was carried out to test sex ratio of the progeny that were induced by the artificial fertilization between normal female and gynogenetic diploid eggs and sex-reversed gynogenetic diploid male sperm. Sex reversed gynogenetic diploid males were produced successfully in gynogenetic diploid female by immersion treatment with $17{\alpha}$-methyltestosterone at the concentration of 10 pub from $13.5{\pm}0.22$ mm to $57.6{\pm}5.1$ mm in total length. In $F_1$ progeny test, matings between normal females and sex-reversed gynogenetic males were not produced all female, but matings between gynogenetic diploid females and sex-reversed gynogenetic males were produced all female populations in six out of eight groups and two lots produced $96.9\%$ and $95\%$ female. These data showed that all-female populations were produced by crossing between gynogenetic diploid female and sex reversed gynogenetic diploid male.
참종개 Iksookimia koreensis의 난발생 및 초기생활사
방인철 ( In Chul Bang ) 한국하천호수학회 2012 생태와 환경 Vol.45 No.1
In the present study, egg development and early life history of Korean spined loach, Iksookimia koreensis, were observed. Adult fish were sampled using spoon nets in Okgye-ri, Hoengseong-gun, Gangwon-do, Korea in July 2010. Eggs were obtained after injecting females with Ovarprim. Eggs were then artificially fertilized in the laboratory using the dry method. Mature eggs were slightly adhesive and transparent with a light yellowish color, and measured 1.40±0.04mm(mean±SD) in diameter. Hatching of the embryo occurred approximately 50 h after fertilization in the water at 23℃, and newly hatched larvae were averaged 4.7±0.21 mm in total length. 5 days after hatching, the averaged total length of larvae was 7.1±0.25 mm and their yolk sacs had been completely absorbed. 17 days after hatching, fish started to enter the juvenile stage and reached 12.2±1.10 mm in total length. 80 days after hatching, the band patterns and external form of juvenile fish were similar to those of adults, and they averaged 31.0±3.98 mm in total length.
북한강 참다슬기, Semisulcospira coreana (v. Martens) 의 번식생태
김대희,방인철,이완옥,백재민,Kim, Dae-Hee,Bang, In-Chul,Lee, Wan-Ok,Baek, Jae-Min 한국패류학회 2012 The Korean Journal of Malacology Vol.28 No.2
북한강에 서식하고 있는 참다슬기의 산란생태를 파악하기 위하여 생식소 발달단계에 따른 생식주기, 성비, 군성숙도, 보육낭유생의 월별변화 등 번식생태와 관련된 생물학적 기초 자료를 조사하였다. 각고에 대한 각경, 전중, 육중의 상관계수 ($R^2$) 는 0.7761-0.8719로 비교적 높은 상관관계를 나타내었다. 참다슬기의 암컷 생식소는 청록색, 수컷 생식소는 옅은 황색을 띠고, 암컷은 유생을 기르는 보육낭 (brood pouch) 을 가지고 있었다. 암수성비는 0.17-0.58의 범위로 계절적 불균형이 심하였으며, 여름철 암컷 비율이 높고 겨울철 암컷비율이 낮았다. 생식소의 조직학적 조사 및 비만지수, 생식소지수 조사 결과 봄, 가을에 두 번의 정점을 나타낸 후 급격히 떨어지는 경향을 보여 생식소 발달은 봄, 가을 연 2회 이루어짐을 알 수 있으며, 암컷 보육낭속의 유생수 월별변화도 연중 두 번의 피크를 나타냈었고, 보육낭속의 유생수는 월 평균 286-975범위로, 11월에 연중 가장 적었고 3월과 9월에 각각 975개, 863개로 연중 가장 많은 유생을 보유하였다. 50%가 성숙하는 군성숙체장은 각고 13.95 mm였으며, 각고 15mm 이상은 모든 개체가 성숙하고 보육낭을 형성하였다. To clarify reproductive ecology of the melania snail Semisulcospira coreana (v. Martens, 1886) in Bukhan River, gonad development, fatness, gonad index, sex ratio, first sexual maturity of population, monthly change of larvae number and developmental stages in brood pouches were investigated by six identification methods. As maturation progrsses, the sex of the snali can be distinguishable easily by color:: the ovary being blue-green and testis light yellow. The sex ratio of female to male individuals over 13.95 mm shell height was significantly different from 1:1 (${\chi}^2$ = 38.45, p < 0.05). The sex ratio of female to male individuals changed drastically according to the season, Based on the monthly variations of fatness, gonad index and histological analysis, spawning occurred twice a year (spring and autumn) and the mean size of matured eggs was $450{\mu}m$ in diameter. The monthly change of larval number in brood pouch showed also two distinct peaks in March and September during the year. The average number of larvae in brood pouches was 286 - 862 individuals. In this study, the number of larvae in the brood pouches were a minimum in December and a maximum in March (975 larvae). The biological minimum size (the size at 50% of group sexual maturity) of the melania snail was 13.95 mm in shell height in females and males. All females over 15 mm in shell height possesed brood pouches.
방인철(In Chul Bang),남윤권(Yoon Kwon Nam),노충환(Noh Choong Hwan),박준택(Joon Taek Park),한경호(Kyoung Ho Han) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.1
한국산 꺽지과 어류 3종에 대한 세포유전학적 기초 자료를 얻기 위하여 염색체 핵형, 적혈구 세포 및 핵의 크기와 DNA 함량을 조사하였다 꺽지 (Coreoperca herzi), 꺽저기 (C. kawamebari) 및 쏘가리 (Siniperca schezeri)의 핵형은 각각 2n=48 (4SM+44A,T), 2n=48(6SM+42A,T) 2n=48(4SM+44A,T) 이었으며, 암·수간 heteromorphic한 성염색체는 발견할 수 없었다. 쏘가리의 적혈구 세포 및 핵의 표면적과 체적은 다른 2종보다 작았다. DNA 함량은 적혈구 세포 크기에서 같은 경향을 보여, 쏘가리가 1.47 Pg/cell로 꺽지 (1.83 pg/cell) 및 꺽저기 (1.85pg/cell)보다 유의하게 작았다. Cytogenetic characteristics of three species in Centropomidae (Coreoperca herzi, C. kawamebari and Siniperca schezeri) were evaluated, based on karyologcial analysis, erythrocytic measurement and genome size estimation using flow cytometry. Modal chromosome number of three species was same as 2n=48. Karyotypes were 4SM+44A·T (NF=52) for Coreoperca herzi, 6SM+42 A·T (NF=52) for C. kawamebari and 4SM+44A·T (NF=52) for Siniperca schezeri. Heteromorphic sex chromosome was not found in both sexes of any species examined. Cellular and nuclear volumes of Siniperca schezeri were smaller than those of other two species. Average amounts of cellular DNA contents estimated by flow cytometry were well coincided with erythrocytic sizes. The estimated genome sizes were 1.83, 1.85 and 1.44 pg/cell for C. herzi, C. kawamebari and S. schezeri, respectively.