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천왕보심단(天王補心丹)의 항우울효과 및 monoamine 대사에 미치는 영향
박종흠,배창욱,전현숙,홍성유,박선동,Park Jong-Heum,Bae Chang-wook,Jun Hyun-Suk,Hong Sung-You,Park Sun-Dong 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.2
Depression is a sort of mental disorder which is very common. To treat depression, many drugs such as TCA, MAOI are developed and used. But they have a lot of side effects, so it needs to develop drugs without side effects or with less side effects. Herbal medicines have been used to treat diseases not only physical but also mental and have less side effects. therefore, it has been thoght the need to develop herbal medicine with antidepressant effect. The purpose of this study was to reseach antidepressant effect and influence on monoamines of chunwangboshimdan thought to have antidepressant according to ancient medical book- donguibogam- and recent reports. We used 'forced swimming test(FST)' to know antidepressant effect of chunwangboshimdan and HPLC to check the influence on monoamines and their metabolites(norepinephrine, dopamine, DOPAC, HVA, serotonin, 5-HIAA) of chunwangboshimdan after divided into cerebral cortex, striatum, hypothalamus and hippocampus. The results were obtained as follows: In the study of antidepressant effect by 'forced swimming test(FST)'method, chunwang boshimdan had a significant antidepressant effect. In the study of influence on monoamines by HPLC, chunwangboshimdan mainly increased dopamine among monoamines and their metabolites(norepinephrine, dopamine, DOPAC, HVA, serotonin, 5-HIAA) significantly in 4 parts of rat's brain above-mentioned. Calculated by turnover ratio formulae of monoamine, chunwangboshimdan has more results than Imipramine. These results suggest that chunwangboshimdan has antidepressant effect that is related with the increase of monoamines by suppressing their metabolism as its mechanism.
정석규,박종흠,지승택,박금주,김해홍,현창기,신현길,Jeong, Seok-Kyu,Park, Jong-Heum,Ji, Seung-Taek,Park, Kum-Ju,Kim, Hai-Hong,Hyun, Chang-Kee,Shin, Heuyn-Kil 한국식품과학회 2000 한국식품과학회지 Vol.32 No.4
Comet assay를 이용하여 방사선 조사육의 조사여부 및 조사량을 판별해내는 방법을 개발하기 위해 1-10 kGy의 감마선 조사량으로 조사한 육조직에서 일어나는 DNA 손상을 측정하였다. Comet assay에 있어 최적의 comet image를 얻기 위해 세포 분리, 세포 lysis 및 전기영동에 대한 여러 조건들을 적용하여 최적의 조건을 확립하였다. DNA 손상도는 관찰되는 comet의 평균 tail length와 tail length에 의해 구분된 4 손상 등급에의 분포, 그리고 그 분포비율에 의해 본 연구에서 제시한 식에 의해 계산한 relative damage index(RDI) 값 등으로 비교 판정하였다. 평균 tail length와 RDI 값은 조사량이 증가함에 따라 증가하여 DNA 손상도가 증가됨을 나타내었으며, 평균 tail length으로는 조사량 간의 차이를 명확히 분별하기가 어려웠던 반면 RDI 값에 의하면 조사여부 및 조사량을 판별하기에 적합함을 알 수 있었다. 국내산 한우육과 수입 냉장육에 대하여 blind test를 실시한 결과 수입육이 높은 DNA 손상도를 나타내었는데 그 RDI 값은 방사선 조사에 의한 값보다는 비교적 낮은 것이어서 수입육의 DNA 손상은 방사선 조사가 아닌 저온처리의 결과인 것으로 판단되었다. 본 연구의 결과로부터 Comet assay가 우육의 방사선 조사여부 및 조사량 판별에 유용한 기술로 응용될 수 있을 것으로 판단되었다. DNA damages in post-mortem bovine muscle samples caused by gamma irradiation at doses of 1 to 10 kGy were determined by Comet assay. When the cell extract was prepared in a physical method and followed by neutral lysis and neutral electrophoresis, the optimal comet images could be obtained. DNA damages were evaluated from the mean tail length, the distributions of comet images in 4 groups divided by tail length and the relative damage index (RDI) values calculated from the distribution pattern. The mean tail length and RDI value were increased by increasing the irradiation dose, and the RDI value was found to be useful as an index for discriminating of irradiation and measuring the irradiated dose. Blind tests using Korean domestic (Hanwoo) and imported beef samples showed a higher RDI value for the latter. However, the value was lower than those of irradiated samples indicating that the cause of DNA damages in the imported beef samples might be not irradiation but low-temperature treatments. It was concluded from the results of this study that the irradiated beef and its irradiated dose could be detected and predicted by Comet assay.
Comet Assay를 이용한 전통발효식품인 배추김치의 항유전 독성효과
지승택(Seung-Taek Ji),박종흠(Jong-Heum Park),현창기(Chang-Kee Hyun),신현길(Heuyn-Kil Shin) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.2
본 연구에서는 우리나라 전통발효식품인 배추김치의 암예방 기능성을 규명하기 위하여 항유전독성을 측정할수 있는 Comet assay(일명 single cell microgel electrophoresis)를 사용하였다. 배추김치를 물 분획, 유기용매(n-hexane, chloroform, ethyl acetate) 분획, 불용성 분획과 우세발효균주들로 분리하였다. 배추에서 분리되어진 물 분획, n-hexane 분획, chloroform 분획, ethyl acetate분획, 불용성 분획은 non-tumoral normal 3T3 세포의 DNA손상에 대하여 항유전 독성효과를 나타내지 못하였다. 그러나 배추에서 분리된 발효균주 bacteria 1과 bacteria 2는 유의성 있게 3T3 세포의 DNA 손상을 감소시키는 항유 전독성 효과를 나타내었으나 bacteria 3, 4, 5는 항유전독성 활성을 나타내지 않았다. This study carried out to elucidate the cancer chemoprevention of Korean native fermented food, baechu kimchi using Comet assay (in other words, single cell microgel electrophoresis). For this purpose, baechu kimchi was fractionated by water, n-hexane, chloroform and ethyl acetate. 5 strains of dominant fermented bacteria were isolated from baechu kimchi. The water fraction, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water insoluble fraction showed no antigenotoxicities in non-tumoral normal 3T3 cells. Among 5 bacteria isolates from baechu kimchi, two isolates bacteria 1 and 2 strongly inhibited genotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in non-tumoral normal 3T3 cells (p<0.05). Bacteria 3, 4 and 5 were also not antigenotoxic.
뢰영성 ( Yong Sung Loi ),권영모 ( Kwon Yeong Mo ),박종흠 ( Park Jong Heum ),박선동 ( Park Seon Dong ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.3
N/A objectives: Exposure to oxidant molecules can generate reactive oxigen species(R0S; for example, H₂O₂, O₂-, OH .). These free radicals can alter DNA, proteins and membrane phospholipids. ROS are responsible for aging and many diseases including cancer, neurodegenerative diseases and cardiovascular diseases. Thus, to prevent against celluar damages associated with oxidative stress, antioxidants are important and many research about antioxidants are being conducted over the world. methods: This study was performed to research the antioxidant activities of Insamgobonwhan(IG) and its component groups in vitro, so intended to know the possibility of Insamgobonwhan(1G) as drug which can prevent aging. The experimental group was divided into 4 groups: Imsamgobonwhan(IG), two dong-two jiwhang(IG-1; 麥門冬, 天門冬, 熱地黃, 生地黃), two dong(IG-2; 麥門冬, 天門冬) and two jiwhang(IG-3; 熱地黃, 生地黃). results: 1. All experimental groups had significant antioxidant activity in linoleic acid system on concentration dose--dependently in comparison with control group. But IG and IG-2 have more effect than IG-1 and IG-3 relatively. 2. DPPH radical scavenging experiment and inhibitory effect experiment on superoxide generation in xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. But IG and IG--1 had more effect than IG-2 and IG-3. 3. Inhibitory effect experiment on superoxide generation induced by xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. But IG and IG-1 had more effect than IG-2 and IG-3. 4. Inhibitory effect experiment on lipid peroxidation reaction in H₂O₂-Fe²system showed that all experimental groups had a dose--dependent effect. But IG and IG--1 had more effect than IG-2 and IG-3. 5. Experiment on lipid peroxidation removal effect showed that all experimental groups had a dose-dependent effect. But IG and IG-2 have more effect than IG-1 and IG-3. 6. Experiment on glutathion activity effect showed that all experimental groups had some effect. But each groups had no significant difference. 7. Experiment on catalase activity effect showed that all experimental groups had some effect. But each groups had no significant difference. conclusion: These results showed that IG has an antioxidant activity, so it seems to have some anti-aging effect. From comparisons of each experimental groups, IG`s antioxidant effect is likely to be associated with IG-1 mostly.