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민병대,정현미,이이내,최인철,안경희,박주현 대한상하수도학회 2016 상하수도학회지 Vol.30 No.6
In order to prevent secondary pollution of tap water, corrosion characteristics are investigated, and corrosion index are calculated using LI and LR to manage corrosiveness. As targeted water treatment plants from 2014 (July, once) to 2015 (July and October, 2 times), 70 plants are selected by making a division for each area and water system. (treated water samples, n=240, raw water samples, n=72). In result of pH analysis, treated water was lower than raw water to 7.12, and 7.29, respectively. LI were investigated in the order of Seomjin river, Nakdong river, Han river, Geum river, to -2.08, -1.24, -1.11, -1.10 (at raw water), and -2.18, -1.59, -1.51, -1.35 (at treated water), respectively. In case of water quality goal value (LI = -1) in Japan as control of corrosiveness, management object was investigated about 83.3%.
민병대,YAMAZAKI Kimiko,KOIZUMI Akira 대한상하수도학회 2011 상하수도학회지 Vol.25 No.4
In small-scale water systems, the measurement of quality of raw water in running water is generally implemented when the quality of water is stable and frequency of measurement is low. However, units such as water temperature and pH, which are easily monitored, are frequently measured. In establishing an improvement plan for a water treatment system, the range of concentration of the target material present in the raw water of the running water provides relevant information. If the concentration of target material can be specified by the quality of water of data items that are measured daily, inverse estimation of the range of concentration is possible as well. In this paper, we took note of manganese in the raw water from Ogasawara-mura, Tokyo, and estimated the manganese concentration in the raw water of the running water for the past five years. Based on the results obtained, we have proposed a manganese removal system, considering the current situation and geographical conditions of Ogasawara-mura.
국내 정수장의 Larson's ratio 특성에 관한 연구
민병대,정현미,안경희,박주현 대한상하수도학회 2016 상하수도학회지 Vol.30 No.5
In many countries in order to manage corrosion of water treatment process, it is currently using Langelier index (LI). However, management of the Larson's ratio (LR) to compare corrosion management and LI which can be generated by the water treatment process is required. In this study, in order to ensure data LR, factors associated with the actual corrosion resistance of water treatment plant was measured. Using the measured data, the model equation can be estimated alkalinity, and using the statutory water quality data, LR and alkalinity is estimated. At comparison of the measured value and estimated value of alkalinity, it appeared in R2 = 0.629, using the statutory water quality data and estimated alkalinity model, LR and alkalinity (Whole water treatment plants : 472) is estimated. Concentration of estimated alkalinity is 0.5 mg/L to 107.5 mg/L (average : 23.2 mg/L), and LR is 0.1 to 10 (average : 1.3). At tendency to corrosion of investigated LR, “No metal tendency” (>0.5) is 39 water treatment plants, 8.26 %, and “corrosion metal tendency” is 433 water treatment plants, 91.74%.
국내 정수장의 Larson's ratio 특성에 관한 연구
민병대,박주현,정현미,안경희 대한상하수도학회 2016 상하수도학회지 Vol.30 No.5
In many countries in order to manage corrosion of water treatment process, it is currently using Langelier index (LI). However, management of the Larson's ratio (LR) to compare corrosion management and LI which can be generated by the water treatment process is required. In this study, in order to ensure data LR, factors associated with the actual corrosion resistance of water treatment plant was measured. Using the measured data, the model equation can be estimated alkalinity, and using the statutory water quality data, LR and alkalinity is estimated. At comparison of the measured value and estimated value of alkalinity, it appeared in R2 = 0.629, using the statutory water quality data and estimated alkalinity model, LR and alkalinity (Whole water treatment plants : 472) is estimated. Concentration of estimated alkalinity is 0.5 mg/L to 107.5 mg/L (average : 23.2 mg/L), and LR is 0.1 to 10 (average : 1.3). At tendency to corrosion of investigated LR, “No metal tendency” (>0.5) is 39 water treatment plants, 8.26 %, and “corrosion metal tendency” is 433 water treatment plants, 91.74%.
정수장 현장제조염소의 브로메이트와 클로레이트의 생성 특성연구
민병대,정현미,김태욱,박주현,Min, Byungdae,Chung, Hyenmi,Kim, Taewook,Park, Juhyun 대한상하수도학회 2015 상하수도학회지 Vol.29 No.5
Although disinfection in drinking water treatment plants provides a safer water supply by inactivating pathogenic microorganisms, harmful disinfection by-products may be formed. In this study, the disinfectant, chlorine, was produced on-site from the electrolysis of salt (NaCl), and the by-products of the disinfection process, bromate and chlorate, were analyzed. The provisional guideline levels for bromate and chlorate in drinking water are $10{\mu}g/L$ and $700{\mu}g/L$, in Korea, respectively. Bromide salt was detected at concentrations ranging from 6.0 ~ 622 mg/kg. Bromate and chlorate were detected at concentrations ranging from non-detect (ND) ~ 45.3mg/L and 40.5 ~ 1,202 mg/L, respectively. When comparing the bromide concentration in the salt to the bromate concentration in the chlorine produced by salt electrolysis, the correlation of bromide to bromate concentration was 0.870 (active chlorine concentration from on-site production: 0.6-0.8%, n=40). The correlation of bromate concentration in the chlorine produced to that in the treated water was 0.866.
국민안전처 「보행환경 개선사업 효과평가 매뉴얼」 제정내용 소개
민병대,장경욱 대한교통학회 2015 교통 기술과 정책 Vol.12 No.6
보행안전 및 편의증진에 관한법률 제11조(보행 환경개선사업의 평가) 특별시장등은 보행환경개선 사업을 시행하였을 때에는 그 사업의 성과등을 평가해야 한다.
Nested PCR을 이용한 Cowpea chlorotic mottle virus 정밀 진단 시스템 개발
민병대(Byung-Dae Min),김영석(Young-Suk Kim),이시원(Siwon Lee),이수헌(Su-Heon Lee) 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4
Cowpea chlorotic mottle virus (CCMV)는 Group IV positive sense single strand RNA virus, Bromoviridae과, Bromovirus속으로 분류하는 식물병원성 바이러스로, 강낭콩(Phaseolus vulgaris), 나비완두(Clitoria ternatea), 담배(Nicotiana tabaccum), 대두(Glycine max), 동부(Vigna unguiculata, Vigna siensis) 및 땅콩(Arachis hypogaea)이 국내로 수입될 경우, 검사를 수행하는 관리급 검역바이러스이다. 본 연구에서는, RT-PCR, nested PCR 및 유전자-삽입 양성대조구를 개발하여, CCMV를 현장에서 신속, 정확하게 진단할 수 있는 정밀검정 시스템을 구현하였다. 본 연구에서 개발한 방법은 지속적으로 현장에서 활용되어 식물검역에 기여할 것이라고 기대된다. Cowper chlorotic mottle virus (CCMV) is the ‘controlled’ quarantine virus as plant pathogenic virus that are classed as group Ⅵ (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.
식물검역 Pelargonium Zonate Spot Virus 진단을 위한 RT-PCR 및 Nested PCR 프라이머 개발
이시원,김창수,이진영,민병대,김영석,김태욱,김진,신용길,노재영 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.6
Pelargonium zonate spot virus (PZSV)는 group IV (+) ssRNA viruses, Bromoviridae에 속하는 식물 병원체로, 일반적으로 토마토, 국화, 아티초크 및 제라늄에 감염된다. 본 연구는 검역 현장에서 PZSV를 신속하고 특이적으로 진단 할 수 있는 PCR module을 개발하는 것을 목적으로 하였다. PZSV를 검출하기 위한 RT-PCR 프라이머 선발 결과, 각각 513 및 320 bp를 증폭하는2개 조합을 선발하으며, 더욱 높은 검출감도로 검출할 수 있을 뿐아니라 RT-PCR을 검증할 수 있는 nested PCR 프라이머 조합을 개발하였다. 또한, 제한효소 Xho I 부위를 삽입한 유전자변형-양성대조구 플라스미드를 설계하여, PCR module에서 대조구로부터 오염을 검증할 수 있도록 개발하였다. 본 연구에서 개발한 PCR module은 토마토, 국화, 아티초크 및 제라늄 등에서 PZSV를 간편, 신속 및 특이적으로 검출하여, 지속적으로 식물검역에 활용할 수 있을 것으로 기대된다. Pelargonium zonate spot virus (PZSV) is plant pathogen, belongs to Bromoviridae, group IV (+) ssRNA viruses that is commonly inspected in tomato, chrysanthemum, artichoke or geranium. This study was aimed to develop PCR module for rapid and specific detection of PZSV in quarantine. In this study, the development of two RT-PCR primer sets are efficiently amplified 513 and 320 bp for detection of PZSV. Highly sensitive nested PCR primer sets were developed for detection of PZSV and verification of RT-PCR. In addition, a modified-positive control plasmid containing the restriction enzyme site XhoI was generated, for verification of contamination from PCR control. The present study demonstrated that diagnostic PCR module is useful for detection of PZSV in plant quarantine.