오제스키병의 생체 조기진단을 위한 돼지 비세포 및 순환백혈구내 면역세포화학적 바이러스 항원동정

문운경,조희택,김순복,서정향,Moon, Oun-kyong,Cho, Hee-tack,Kim, Soon-bok,Sur, Jung-hyang 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2

Forty day old piglets were intranasally inoculated with 2ml of Aujeszky's disease virus (NYJ-1-87 strain, $10^{7.0}$ $TCID_{50/0.2}ml$), and the viral antigens were detected in nasal and circulating white blood cells for 20 days after inoculation by immunocytochemical method. Antibody titers in the blood were also detected by neutralizing test and Aujeszky's disease serodiagnostic kit(Choong Ang) in this periods. 1. Viral antigens were detected by the immunocytochemical technigue, and positive reactions were observated in nasal cells from the 2nd to the l0th days after inoculation and circulated white blood cells from the 4th to the 12th days after inoculation. 2. In neutralization test antibodies levels showed titers of 2 on the 8th day, 8 on the l5th day, 16 on the 18th day and 32 on the 20th day after inoculation. In serodiagnostic kit test positive reactions were observed after the 15th day after inoculation.

오제스키병의 생체 조기진단을 위한 면역세포화학, In situ hybridization 및 전자현미경적 연구

문운경,김순복,서정향,송근석,노환국,Moon, Oun-kyong,Kim, Soon-bok,Sur, Jung-hyang,Song, Geun-suk,Nho, Whan-gook 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4

The purpose of this study was to establish early diagnostic methods for the detection of Aujeszky's disease viral antigens and nucleic acid in nasal cells, and buffy coats from experimentally infected living pigs by a combination of immunocytochemistry, in situ hybridization with digoxigenin(DIG)-labled probe and electron microscopy. Forty days old piglets were inoculated intranasally with $10^{7.0}TCID_{50}$ of Aujeszky's disease virus (ADV, NYJ-1-87 strain). The viral antigens and nucleic acid of ADV were detected in nasal cells, and buffy coat for 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopical method. The results were compared with conventional methods such as a porcine Aujeszky's disease serodiagnostic(PAD) kit, neutralization test(NT) and virus isolation. 1. The viral antigens, nucleic acids and capsids of ADV were detected in nasal cells, buffy coats from 3 days to 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopy, respectively. 2. When viral antigens were detected by the immunocytochemical technique, a diffuse brown deposit was observed in the nucleus and cytoplasm of nasal cells, buffy coats and PK-15 cells under a microscope. 3. DIG-labeled DNA probe was prepared by amplification of conserved sequence of recombinant ADV-gp50 clone with polymerase chain reacction. When ADV-DNA was detected by ISH with DIG-labeled probe, purplish blue pigmentation were observed in the nuclei and cytoplasms of ADV-infected cells under a microscope. Positive signals were observed in nasal cells and in the buffy coat and PK-15 cells at the first day after inoculation. 4. Where ADV-capsids were detected by transmission electron microscopical method, aggregation of capsids was observed in the nuclei and cytoplasms of nasal cells, buffy coats and PK-15 cells. The results suggested that these methods were considered as the highly sensitive and reliable tools for rapid and confirmative diagnosis of Aujeszky's disease in living pigs.

2014년 국내 발생 HPAI(고병원성 조류인플루엔자)의시,공간 군집 분석

문운경 ( Oun Kyong Moon ),조성범 ( Seong Beom Cho ),배선학 ( Sun Hak Bae ) 한국지리정보학회 2015 한국지리정보학회지 Vol.18 No.3

본 연구는 질병역학의 관점에서 2014년 발생한 HPAI(H5N8)의 시간적 분포와 공간적 분포 그리고 시·공간을 동시에 고려한 분포를 지리정보시스템과 연계하여 분석함으로써 2014년 발생한 HPAI의 전파 및 확산 특징을 알아보고자 한다. 분석 결과 2014년 HPAI는 시간적으로는 모두 3번의 파동을 형성하였으며, 공간적으로는 경기도·충청북도·충청남도가 인접하는 지역, 전라북도의 곰소만 일대, 전라남도의 영암과 나주 등 영산강과 인접한 지역에서 높은 밀도를 보였다. 시·공간적으로도 공간 밀도가 높은 충청북도 음성지역, 전라북도 부안·고창지역, 나주지역에서 군집이 형성되었다. 다만, 충청북도 음성·진천, 충청남도 천안, 경기도 안성·이천 지역과 전라남도 영암 지역에서는 공간적인 밀도는 높음에도 불구하고 시간적인 범위가 넓음으로써 시·공간 군집이 형성되지 못하였다. 이는 이들 지역의 방역에 문제가 있음을 의미한다. 반면에 곰소만과 인접하고 있는 전라북도 부안·고창·장수 지역은 시·공간 군집이 형성됨으로써, 상대적으로 효과적인 방역이 수행되었다고 볼 수 있다. Outbreaks of highly pathogenic avian influenza(HPAI) subtype H5N8 have occurred in Korea, January 2014 and it continued more than a year until 2015. And more than 5 million heads of poultry hads been damaged in 196 farms until May 2014. So, we studied the spatial, temporal and spatio-temporal patterns of the HPAI epidemics for understanding the propagation and diffusion characteristics of the 2014 HPAI. The results are expressed using GIS. Throughout the study period three epidemic waves occurred over the time. And outbreaks made three clusters in space. First spatial cluster is adjacent areas of province of Chungcheongbuk-do, Chungcheongnam-do and Gyeonggi-do. Second is Jeonlabuk-do Gomso Bay area. And the last is Naju and Yeongam in Jeollanam-do. Also, most of spatio-temporal clusters were formed in spatially high clustered areas. Especially, in Gomso Bay area space density and spatio-temporal density were concurrent. It means that the effective prevention activity for HPAI was carried out. But there are some exceptional areas such as Chungcheongbuk-do, Chungcheongnam-do, Gyeonggi-do adjacent area. In these areas the outbreak density was high in space but the spatio-temporal cluster was not formed. It means that the HPAI virus was continuing inflow over a long period.

닭 Leucocytozoonosis의 병리학적 소견

문운경 ( Oun Kyong Moon ),김순복 ( Soon Bok Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

Pathological findings of leucocytozoonosis naturally occurred in Korea are described. Most of the affected chickens showed paleness of comb and wattle, egg drop, approximately 20 to 30% mortality, green diarrhea, anorexia and weakness. On the blood smear merozoits and gametocytes were detected. At necropsy, petechial and ecchymotic hemorrhages in liver, kidney, heart, muscle, and splenomegaly were frequently observed. Microscopically schizonts with fine granular merozoits were often detected in kidney, intestine, brain, lung, myocardium, muscle, liver with cenrilobular fatty change, and spleen.

동물의 인플루엔자 발생상황 및 역학

문운경 ( Oun Kyong Moon ) 한국수의병리학회 2009 학술대회 Vol.13 No.-

전북 정읍지역 젖소농장 결핵병 집단 발생에 대한 역학조사

윤하정,문운경,김연주,조범준,이수두,이정원,이상진,Yoon, Hachung,Moon, Oun-Kyong,Kim, Youn-Ju,Cho, Bum-Joon,Lee, Soo-Doo,Lee, Jeong-Won,Lee, Sang-Jin 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.4

The present study describes an investigation on an epidemic of Tuberculosis (TB) which has been occurred among dairy cattle farms in Jeongeup, Jeollabuk-do since 2007. The investigation was performed in three ways as follows: 1. Collecting information about bovine TB outbreaks using investigation reports, an on-the-spot and tracing-back investigations; 2. Analyzing the outbreak pattern; 3. Establishing hypothesis and performing statistical analysis on potential risk factors. In the early 2000s, TB outbreaks were sporadically reported in beef cattle, and only a small number ($1{\sim}2$) of reactors was confirmed in each of outbreak farms. The number of TB outbreaks has been suddenly increased from 2007, mainly in dairy cattle farms. And these outbreaks were temporarily clustered during the period, from March 2007 to April 2009 (relative risk, RR = 13.7, p < 0.001). And two spatial clusters of which radiuses were 0.3 km (RR = 6.9, p < 0.001) and 0.9 km (RR = 3.6, p < 0.01). The analysis to find risk factors was performed on 99 dairy farms (21 outbreaks), which are located in the most seriously affected village during 2007-2009. Middleman (odds ratio, OR = 47.4, p < 0.05) and raw milk collecting system (OR = 6.9, p < 0.05) were recognized as with the highest association. Considering the fact that all the outbreak farms except one had their own manure composting tank, it might be that the manure containing pathogen was leaked from tank and transmitted to other farms by fomites such as middleman or raw milk collecting system.

국내분리 오제스키병 바이러스로 비강접종한 야생집쥐(Rattus norvegicus)의 병리학적 소견 및 in situ hybridization에 의한 바이러스 동정

송근석,문운경,정창근,김순복,Song, Geun-seok,Moon, Oun-kyong,Jeong, Chang-geun,Kim, Soon-bok 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

The present study was carried out to investigate the pathogenicity and pathogenesis of wild rats(Rattus norvegicus), trapped in nature, intranasally infected with Aujeszky's disease virus(ADV/NYJ-1-87) by histopathology, immunohistochemistry and in situ hybridization(ISH). Fifteen rats inoculated intranasally were roughened haircoat, anorexia, listlessness, and depression second day after inoculation, and three rats died in 66-72 hours. Eight rats showed severe pruritus at the face that was accompanied by frequent face-washing movements of the forelegs, and then became violent and spasmodic for an hour or until they died. Four rats slowly recovered after showing mild clinical signs of the disease. Microscopic lesions in infected rats were characterized by meningitis, perivascular round cell infiltration, focal gliosis, and neuronal degeneration and necrosis. And intranuclear inclusion bodies were frequently detected in the cerebral cortex and medulla. Positive reaction to ADV by immunohistochemistry and ISH were detected in the following areas : trigermimal ganglion, brain, tonsil, nasal mucosa, spleen, lung and liver. The result has suggested that ADV intranasally infected in wild rats is followed by replication in epithelial calls of nasal mucosa and tonsil, then invade local lymph nodes by way of the lymphatics. It is also believed that the virus invades bipolar olfactory cells and trigerminal ganglion; and then spread into central nervous system.

소 질병 검출을 위한 혈청학적 검사의 민감도 평가

이상진,문운경,박선일,Lee, Sang-Jin,Moon, Oun-Kyong,Pak, Son-Il 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.1

Animal disease surveillance system, defined as the continuous investigation of a given population to detect the occurrence of disease or infection for control purposes, has been key roles to assess the health status of an animal population and, more recently, in international trade of animal and animal products with regard to risk assessment. Especially, for a system aiming to determine whether or not a disease is present in a population sensitivity of the system should be maintained high enough not to miss an infected animal. Therefore, when planning the implementation of surveillance system a number of factors that affecting surveillance sensitivity should be taken into account. Of these parameters sample size is of important, and different approaches are used to calculate sample size, usually depending on the objective of surveillance systems. The purpose of this study was to evaluate the sensitivity of the current national serological surveillance programs for four selected bovine diseases assuming a specified sampling plan, to examine factors affecting the probability of detection, and to provide sample sizes required for achieving surveillance goal of detecting at least an infection in a given population. Our results showed that, for example, detecting low level of prevalence (0.2% for bovine tuberculosis) requires selection of all animals per typical Korean cattle farm (n = 17), and thus risk-based target surveillance for high risk groups can be an alternative strategy to increase sensitivity while not increasing overall sampling efforts. The minimum sample size required for detecting at least one positive animal was sharply increased as the disease prevalence is low. More importantly, high reliability of prevalence estimation was expected with increased sampling fraction even when zero-infected animal was identified. The effect of sample size is also discussed in terms of the maximum prevalence when zero-infected animals were identified and on the probability of failure to detect an infection. We suggest that for many serological surveillance systems, diagnostic performance of the testing method, sample size, prevalence, population size, and statistical confidence need to be considered to correctly interpret results of the system.

오제스키병바이러스 인공감염 한국재래산양의 병리학적 소견 및 절편내 in situ hybridization 바이러스 동정

김순복,송근석,문운경,정창근,Kim, Soon-bok,Song, Geun-suk,Moon, Oun-kyong,Jeong, Chang-geun 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.2

Aujeszky's disease virus(ADV) was inoculated intranasally into the Korean native goats to investigate pathological findings and pathogenesis of ADV infection by using of histological and immunohistochemical methods and in situ hybridization(ISH). Clinical signs of salvation, pyrexia, pruritus and staggering were followed by death with five days after inoculation, Pathoanatomical findings were edema of the lung and the urinary bladder with hemorrhage and congestion, petechial hemorrhages on the endo-and epicardium, renal congestion, moderate splenomegaly and cystic edema. Main microsocpic lesions observed in all infected goats were confined to the CNS and charcterized by perivascular cuffing with lymphocytes and macrophages, focal gliosis, neuronal degeneration and necrosis, and intranuclear inclusion bodies in the neurons and glial cells. Positive reactions to ADV were detected more frequently in the nuclei than in the cytoplasms of infected nerve cells in the CNS by immunohistochemistry and ISH. Frequenctly localized sites of ADV in the CNS were olfactory bulb, prietal cortex, callosal sulcus and corpus callosum. Positive reactions were also detected in the tonsillar epithelium, and alveolar macrophage and sloughed epithelium of the lung.

부분예산분석을 이용한 오제스키병 발생 농가의 경제적 손실 추정

박선일,박최규,문운경,윤하정,이병용,이상진,Pak, Son-Il,Park, Choi-Kyu,Moon, Oun-Kyong,Yoon, Hachung,Lee, Byeong-Yong,Lee, Sang-Jin 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.4

Aujeszky's disease (AD) is a respiratory, infectious viral illness associated with high mortality, especially in neonatal piglets and has frequently been considered an economically important disease in many endemic countries. Although AD is still occurring in a geographically defined region in Korea, little attention has been paid to the economics of AD. In this study, partial budget technique was used to develop a simulation model to measure financial losses following the disease epidemic in a swine operation utilizing stochastic or deterministic parameters from the literatures and the index case herd of AD occurred in 2005, where available and applicable. For the infected case herd with a 12500-pig, the total economic loss for this operation was estimated to be about 199 million Korean won (95% confidence interval [CI] 148,645,000-250,741,000). Given net loss due to death of a pig at sow level was 119,000 won, total loss for the case herd with 1200 sows accounted for 143 million won (95% CI 92,599,000-193,729,000). The net loss of the death of one pig at growing and fattening level resulted in loss of 46,000 won (95% CI 40,000-53,000) and 126,000 won (95% CI 122,000-131,000), respectively. Taking into account for the number of pigs raised in the case herd, total loss amounted to 8 million won (95% CI 7,167,000-9,347,000) and 12 million won (95% CI 11,959,000-12,891,000), for growers and fatteners, respectively, assuming 63% of saved feed intake when a pig dies halfway through the respective period. Under the model's assumptions, suckling pig mortality was the major factors of loss in estimating the economic consequences (approximately 71.8% of the total loss). The high economic losses of a herd infected with AD suggest that the effective and region-specific control measures should be implemented in disease endemic foci.