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록용약침액(鹿茸藥鍼液)의 위암세포주(胃癌細胞柱)에 대한 Oligonucleotide Chip 분석
류성현 ( Sung Hyun Ryu ),이경민 ( Kyung Min Lee ),이봉효 ( Bong Hyo Lee ),임성철 ( Seong Chun Lim ),정태영 ( Tae Young Jun ),서정철 ( Jeung Chul Seo ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.2
Objectives: It has Long been known about the osteogenic effect of CPC-HAS(cervi parvum cornu herbal-acupuncture solution) on bone tissues. However, it has not been determined the effect of CPC-HAS on cancer cells. The purpose of this study is to screen the CPC-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell Lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods: CPC-HAS was prepared by boiling and stored at -70℃ until use. Cells were treated with various concentrations of CPC-HAS (0.1, 0.5, 1.5, 10, 20 ㎎/㎖) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5 ㎎/㎖ of CPC-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip(Human genome U133 Plus 2.0., Affimatrix Co.). Results: It has no cytotoxic effects on SNU484 cell in all concentrations(0.1, 0.5, 1.5, 10, 20 ㎎/㎖). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 5 while, the number of more than twofold down-regulated genes was 10. Conclusions: This study showed the screening of CPC-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray. The screened genes will be used for the better understanding of the therapeutic effects of CPC-HAS on cancer fields.
녹용약침액(鹿茸藥鍼液)의 간암세포주(肝癌細胞柱)에 대한 DNA 및 단백질 발현(發顯)
류성현,이경민,이봉효,임성철,정태영,서정철,Ryu, Sung-Hyun,Lee, Kyung-Min,Lee, Bong-Hyo,Lim, Seong-Chul,Jung, Tae-Young,Seo, Jung-Chul 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2
Objective : It has long been known about the osteogenic effect of CPC-HAS on bone tissues. However, it has not been determined the effect of CPC-HAS on cancer cells. The purpose of this study is to screen the CPC-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cells. Oligonucleotide microarray and proteomics approaches were employed to screen the differential expression genes. Methods : CPC-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of CPC-HAS (0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of CPC-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip(Human genome Ul33 Plus 2.0., Affimatrix Co.). For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : It has no cytotoxic effects on both HepG2 cell in all concentrations(0.l, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, the number of more than twofold differentially regulated known genes was 23 with 5 up-regulated and 18 down-regulated genes in HepG2 cells. In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. Two down-regulated proteins were aldehyde dehydrogenase 1 and enolase 1, and up-regulated protein was fatty acid binding protein 1 by 1.5mg/ml of CPC-HAS. Discussion : This study showed the screening of CPC-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray and proteomic analysis. The screened genes will be used for the better understanding of the therapeutic effects of CPC-HAS on cancer fields.
시간사용중재가 뇌졸중 환자의 생활만족도 및 자아존중감에 미치는 영향
류성현(Ryu, Sung-Hyun),박수진(Park, Su-Jin),김민혁(Kim, Min-Huck),황도연(Hwang, Do-Yeon) 대한신경계작업치료학회 2020 재활치료과학 Vol.9 No.3
목적 : 본 연구는 뇌졸중 환자를 대상으로 8주간 실시된 시간사용중재가 뇌졸중 환자의 생활만족도 및 자아존중감에 미치는 영향을 알아보았다. 연구방법 : 본 연구는 2019년 7월부터 10월까지 뇌졸중 환자 21명을 대상으로 실험군과 대조군으로 나누어 실시하였다. 생활만족도의 변화는 생활만족도 척도(Life satisfaction scale)를 사용하였고, 자아존중감의 변화는 자아존중감 척도(Self-esteem scale)를 사용하였다. 결과 : 첫째, 실험군과 대조군 각각 중재 전과 후를 비교한 결과, 생활만족도와 자아존중감 모두 통계학적으로 유의한 차이를 보였다(p<.05). 둘째, 중재 후 실험군과 대조군의 생활만족도와 자아존중감을 비교한 결과, 통계학적으로 두 집단 간 유의한 차이를 보였다(p<.05). 결론 : 본 연구의 결과 시간사용중재가 뇌졸중환자의 생활만족도와 자아존중감에 긍정적인 영향을 미쳤으며, 따라서 의미있는 작업 수행의 참여가 뇌졸중 환자의 정신건강과 생활의 질을 향상시킬 수 있을 것이다. Objective : This study investigated the effects of time-use intervention, conducted during 8 weeks, on the life satisfaction and self-esteem of stroke patients. Methods : Twenty-one stroke patients who were divided into an experimental group (n=10) and a control group (n=11), participated in this study from july 2019 to october 2019. Life satisfaction changes were measured using the Life satisfaction scale. Self-esteem changes were measured using the Self-esteem scale. Results : First, comparison between pre and post intervention showed a statistically significant difference in life satisfaction and self-esteem (p<.05). Second, comparison between post intervention groups showed a statistically significant difference in life satisfaction and self-esteem (p<.05). Conclusion : Based on the results from this study, we found that time-use intervention has a positive impact on life satisfaction and self-esteem. Therefore, participating in meaningful occupation performance will improve the mental health and quality of life of stroke patients.
내부격벽으로 일체화된 2열 소일시멘트벽체와 수동격벽을 이용한 연약지반용 자립식 흙막이 벽체 공법(BSCW 공법) (건설신기술 제915호)
김인철(In Cheol Kim),이치호(Chi Ho Lee),김영윤(Young Yun Kim),김철환(Cheol Hwan Kim),이기철(Ki Cheol Lee),박정규(Jung Gyu Park),장순호(Soon Ho Jang),임시내(Si Nae Lim),류성현(Sung Hyun Ryu),고호성(Ho Seong Ko),이현민(Hyun Min Lee) 대한토목학회 2021 대한토목학회지 Vol.69 No.10