Phorbol Ester로 유도된 COX-2의 발현에 미치는 녹차의 항산화성분 EGCG의 억제 효과와 그 분자 생물학적 작용기전

나혜경,서영준 대한암예방학회 2003 Journal of cancer prevention Vol.8 No.4

전반적 발달장애(자페증) 아동에 대한 긍정적 사회행동지도를 위한 사회기술훈련 적용 사례

나혜경 한국정신건강사회복지학회 1999 한국정신건강사회복지학회 학술발표논문집 Vol.1999 No.11

식이 ω3 지방 섭취가 발달과정의 흰쥐 뇌지질에 미치는 영향

나혜경,조성희 대구효성가톨릭대학교 응용과학연구소 1993 응용과학연구논문집 Vol.2 No.-

식이 ω3지방 섭취가 발달과정의 뇌지질 지방산 축적에 미치는 영향을 조사하기 위해 ω3지방산이 다량 함유된 고등어유, 들깨유와 비교군으로 콩기름, 쇠기름, 채종유의 5종류의 식이지방을 식이중에 10%로 제조하여 섭취게 한 후 태아, 수유, 이유기를 거치는 동안의 뇌지질 지방산 구성변화를 추적하고 cholesterol 함량과 acetylcholinesterase활성을 측정하였다. 그 결과 뇌조직 지방산중 포화와 불포화지방산은 모두 지속적으로 축적되며 C18:2(ω6)나 C18:3(ω3)같은 short-chain 전구체의 비율은 상대적으로 감소되는 반면 그 long chain유도체의 비율은 증가하여 정량적으로 축적되었다. 특히 C22:6(ω3)의 축적이 타지방산에 비해 많으며 고등어유군에서 계속 높은 경향으로 이는 C20-22 (ω3)지방산의 incoproation율이 타 식이지방의 주요지방산보다 뚜렷이 높음을 뜻한다. 반면 들깨유군에서는 C20:5(ω3)의 elongation은 활발하게 C22:6(ω3)까지의 elongation은 원할치 않다는 것을 나타낸다. Cholesterol함량과 acetycholinesterase 성분은 전 발달과정 동안에 증가하는 경향이었으며 cholesterol 함량은 고등어유군과 쇠기름군에서 높았고 acetylcholinesterase 활성은 각 군간에 큰 차이가 없었다. Male nad female Sprague-Dawley rats(~70g) were fed five different diets containing various dietary fats and at the level of 10%(w/w). Dietary fats used were mackerel oil(MO), perilla oil(PO), soybean oil(SO), repeseed oil(RO), and beef tallow(BT). After 34days of feeding experimental diets, when rats weighed about 200g, male and female rats were mated. Experimental diets were continued throughout pregnancy and lactation of female rats and weanling period of the offsprings. Fatty acid composition and cholesterol content of brain lipid were measured as well as activities of acetylcholinesterase during fetal(day-10, -5), neonate(day 0) and postnatal (day 17, 26, 39) periods. Both saturated and unsaturated fatty acids of brain lipid were progressively elongated from fetal(day-10) to postnatal(day 39). Levels of ω3, ω6 and ω9 fatty acid were notably high in MO, SO, and BT group respectively. In PO group, the level of C20:5(3ω) was higher while the level of C22:6(ω3) was almost the same, as other groups, suggesting that elongation of C18:3(ω3) to C20:5(ω3 is active but not to C22:6(ω3). On the other hand, C22:6(ω3) in MO was very well incorporated in to brain lipid as such. Cholesterol content and acetylcholinesterase activity were increased during the whole period of development. Cholesterol content was highest in MO group. However, acetylcholinesterase activities did not between groups.

Helicobacter pyloriInduces Hypermethylation of CpG Islands Through Upregulation of DNA Methyltransferase: Possible Involvement of Reactive Oxygen/Nitrogen Species

나혜경,우정화 대한암예방학회 2014 Journal of cancer prevention Vol.19 No.4

Helicobacter pyloriinfection has been considered to be one of the major factors implicated in etiology of gastric cancer. Aberrant DNAmethylation accounts for epigenetic modifications induced by H. pylori. H. pylori-induced hypermethylation has been linked toenhancement of the rates of metastasis and recurrence in gastric cancer patients. H. pylori-induced gene hypermethylation has beenknown to be associated with inflammation. However, the molecular mechanisms underlying H. pylori-induced hypermethylation remainlargely unknown. This review highlights possible involvement of reactive oxygen/nitrogen species in H. pylori-induced hypermethylationand gastric carcinogenesis.

15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis in Ha-ras-transformed Human Breast Epithelial Cells by Targeting IκB kinase–NF-κB Signaling

나혜경,Hongkyung Yang,서영준 대한암예방학회 2020 Journal of cancer prevention Vol.25 No.2

15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), an endogenous ligand for PPARγ, has differential effects on cancer cell proliferation and survival depending on the dose and the type of cells. In the present study, we have investigated the effects of 15d-PGJ2 on apoptosis of the Ha-ras transformed human breast epithelial (MCF10A-ras) cells. When MCF10A-ras cells were treated with 15d-PGJ2 (10 μM) for 24 hours, they underwent apoptosis as evidenced by characteristic morphological features, an increased proportion of sub-G0/G1 cell population, a typical pattern of annexin V/propidium iodide staining, perturbation of mitochondrial transmembrane potential (Δψm), and cleavage of caspase-3 and its substrate PARP. A pan-caspase inhibitor, Z-Val-Ala-Asp (OCH3)-fluoromethyl ketone attenuated cytotoxicity and proteolytic cleavage of caspase-3 induced by 15d-PGJ2. The 15d-PGJ2-induced apoptosis was accompanied by enhanced intracellular accumulation of reactive oxygen species (ROS), which was abolished by the antioxidant N-acetyl-L-cysteine (NAC). 15d-PGJ2 inhibited the DNA binding activity of NF-κB which was associated with inhibition of expression and catalytic activity of IκB kinase β (IKKβ). 15d-PGJ2-mediated inhibition of IKKβ and nuclear translocation of phospho- p65 was blocked by NAC treatment. 9,10-Dihydro-PGJ2, a non-electrophilic analogue of 15d-PGJ2, failed to produce ROS, to inhibit NF-κB DNA binding, and to induce apoptosis, suggesting that the electrophilic α,b-unsaturated carbonyl group of 15d-PGJ2 is essential for its pro-apoptotic activity. 15d-PGJ2-induced inactivation of IKKβ was also attributable to its covalent thiol modification at the cysteine 179 residue of IKKβ. Based on these findings, we propose that 15d-PGJ2 inactivates IKKβ–ΝF-κB signaling through oxidative or covalent modification of IKKβ, thereby inducing apoptosis in Ha-ras transformed human breast epithelial cells.

전반적 발달장애 ( 자폐증 ) 아동에 대한 긍정적 사회행동지도를 위한 사회기술훈련 적용 사례

나혜경 한국정신건강사회복지학회 1998 한국정신건강사회복지학회 학술발표논문집 Vol.1998 No.10

완벽주의와 SNS중독의 관계: 불확실성에 대한 인내력 부족과 경험회피의 이중매개 효과

나혜경,박원주 인문사회 21 2022 인문사회 21 Vol.13 No.5

완벽주의와 SNS중독의 관계:불확실성에 대한 인내력 부족과 경험회피의 이중매개 효과나 혜 경*ㆍ박 원 주** 연구 목적: 본 연구는 인스타그램 혹은 페이스북을 사용 중인 전국 성인 남녀를 대상으로 완벽주의와 SNS중독의 관계에서 불확실성에 대한 인내력 부족과 경험회피의 이중매개효과를 알아보기 위하여 수행되었다. 연구 방법: 이를 위해 SNS를 사용 중인 성인 남녀 263명을 대상으로 다차원적 완벽주의 척도, SNS중독 경향성 척도, 불확실성에 대한 인내력 부족 척도, 경험회피 척도를 사용하여 설문조사를 실시하였다. 연구 내용: 연구결과 첫째, 완벽주의, SNS중독, 불확실성에 대한 인내력 부족, 경험회피의 상관분석 결과 자기지향 완벽주의와 경험회피, SNS중독을 제외한 모든 변인들의 상관이 유의하였다. 둘째, 타인지향/사회부과 완벽주의와 SNS중독의 관계에서 불확실성에 대한 인내력 부족과 경험회피가 이중 매개하는 것을 확인하였다. 결론 및 제언: 이를 통해 SNS중독의 취약성이 높은 타인지향/사회부과 완벽주의자의 치료적 개입 방향을 제안하였다. 본 연구는 SNS중독에 특정적이지 않지만 범진단적으로 사용할 수 있다는 의의가 있다. 추후 균일한 연령대별 분포와 함께 SNS 종류를 다양화한 후속 연구가 필요하다. 핵심어: 완벽주의, SNS중독, 불확실성에 대한 인내력 부족, 경험회피, 이중매개효과 □ 접수일: 2022년 7월 21일, 수정일: 2022년 8월 10일, 게재확정일: 2022년 8월 20일* 주저자, 한양사이대학교 휴먼서비스대학원 상담 및 임상심리전공 석사과정(First Author, Master’s Course, HanYang Cyber Univ., Email: kellyna87@naver.com)** 교신저자, 한양사이버대학교 교수(Corresponding Author, Professor, HanYang Cyber Univ., Email: parkwj@hycu.ac.kr)

再活 속에 살다 외 2편

나혜경 문학과경계사 2004 문학과경계 Vol.4 No.1

갑상선 결절내의 경피적 알코올 주입치료

나혜경 대한영상의학회 1989 대한영상의학회지 Vol.25 No.6

Percutaneous alcohol injection(PAI) is a new sclerosing method for treatment of diseases such as cyst malignant tumor and benign neoplasm. Percutaneous alcohol injection of 26 cases (adenomatous goiter 23 cases follicular adenoma 2 cases & recurrent papillary carcinoma 1 cases) of thyroid nodules were done with 23-G needle under the guidance of ultrasonography. After then we followed up thyroid function test ultrasonographical size and volume of nodules cytologic examination and complications of treatment. The results were as follows: 1. With 0.5-1cc alcohol injection the mean volumes of thyroid nodules were reduced after PAI to 59% (N=26) at 1 month and to 33% (N=14 cases of additiohal PAI) at 6 months of the base line volume (100%) before PAI. 2. There were no significant changes at 1 and 6 months follow-up of TFT after PAI in all cases as compared with base line studies 3. In conclusion ultrasonic guided PAI for thyroid nodule is simple useful and cost-effective method and recommendable in cases of mixed and solid nodules resistant to medical treatment refusal of surgery because of cosmetic problem and recurrent malignant tumors.

기관내관의 기낭압 조정이 수술 후 인후통에 미치는 영향

나혜경,윤혜상 한국간호과학회 2008 한국간호과학회 학술대회 Vol.2008 No.10