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랩-베일을 이용한 양돈용 TMR 발효사료 제조 시스템 개발
김혁주,유병기,이성현,유지수,서형덕,Kim, Hyuck-Joo,Yu, Byeong-Kee,Lee, Sunghyoun,Yu, Ji-Su,Seo, Hyoung-Duk 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.3
In this study, we developed a fermented TMR producing system which could a continuous operation of baling and wrapping the mixed TMR. The baling part has fixed chamber of dia. 900 mm and width 900 mm. And the the turn-table type wrapping part is very compact compared to conventional one in market. The test result were: The absorbed power of the prototype in working was 9.0~15.7 kW. The combined baling and wrapping performance was over 20 bales/hr. The leakage in the process was around 1.9 kg for making a 350 kg TMR bale. The diameter of wrapped TMR bale was 900~950 mm, and its weight was 350 kg in average. Therefore the density was $718kg/m^3$, and it was very high density to the inputted TMR. The pH of the fermented TMR was 5.0~5.6 which was similar to the fermented haylage, also the TMR had no butyric acid's odor.
돼지증식성회장염 신속검진 기술개발(1) -돼지 분변에서의 로소니아균 검출을 위한 항원, 항체 준비-
김혁주 ( Hyuck Joo Kim ),홍종태 ( Jong Tae Hong ),유병기 ( Byeong Kee Yu ),김기영 ( Gi Young Kim ),이진주 ( Jin Ju Lee ),김석 ( Suk Kim ) 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.6
Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods: To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results: The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and SO PPE negative samples. Conclusions: The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis.
김혁주(Hyuck-Joo Kim),전형순(Hyung-Soon Jun),유병기(ByeongKee Yu) 한국콘텐츠학회 2020 한국콘텐츠학회논문지 Vol.20 No.3
정부의 축산업 및 축산차량 등록제 실시로 인하여, 양돈농가에서는 의무적으로 출입구에 차단장치를 설치하여야 하며 출입기록을 관리하여야 한다. 또한, 양돈장의 생물보안을 위해서도 출입인원과 차량, 동물 등의 양돈장 출입과 소독기록 등을 관리하여야한다. 본 연구에서는, RFID와 웹서버 시스템을 이용한 양돈장 출입차단 및 기록장치를 개발하였다. 사람의 출입관리를 위하여 13.56MHz의 접촉식 RFID 시스템을 개발하였으며, 차량 출입관리를 위해서는 900MHz RFID 시스템을 이용한 차량인식 시스템을 개발하였다. 시험결과 시스템 작동, 인식, 웹서버 데이터 저장 등의 성능이 양호한 것으로 판단되었다. 이후, 차량 출입시스템과 사람 출입 및 소독기록장치를 연동하여 시험을 실시하였으며, 기록된 데이터는 전용 프로그램을 이용하여 웹서버의 DB에 저장하였다. 성능시험결과, 출입관리와 데이터관리에 문제가 없는 것으로 판단되었다. As a result of reenforcement of livestock industry licensing and livestock related vehicle registration system, pig farm are mandatory for setting stop bar before pig farm entrance and maintaining the records of inlet and outlet to and from pig farm. Also, the first step to the pig farm biosecurity is the control of entrance human, vehicles, animals and the related records including entrance and sterilization. In this study, the pig farm entrance control system using RFID technology and web-server system was developed. For human entrance, contact type RFID reading system(13.56MHz) was developed. And the 900MHz RFID system was used to detect vehicle entrance. The test result shows that system actuating, recognition, saving records to the web server was successful. Then, the full system of human entrance recording unit, vehicle entrance recording unit, entrance control system, human sterilizing booth containing a tablet for inputting visitor’s record and sterilization record was tested. The records were wrote to web server DB through the data management web-program. Performance test shows the entrance control and data management in server was successfully operated.
RFID이용 전자식 모돈 개체별 액상급이기 개발(1) -RFID를 이용한 모돈 액상급이기 제작 및 성능시험-
김혁주 ( Hyuck Joo Kim ),홍종태 ( Jong Tae Hong ),유병기 ( Byeong Kee Yu ),김상철 ( Sang Cheol Kim ),최규홍 ( Kyu Hong Choi ),장흥희 ( Hong Hee Chang ) 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.6
Purpose: In EU, the animal welfare regulations are strengthening. Also, in Korea, for strengthening of animal protection laws, animal welfare farm certification scheme is being prepared to enforce. To get the certification of animal welfare farm, especially for sows, individual specific feeding in sow group is necessary in accordance with the animal welfare regulation. Also, liquid feeding is reported to be more effective for farrowing as well as dry sow. Therefore, in this study, a electronic liquid sow feeder using RFID which could be fed to farrowing sow simultaneously is developed. Methods: We manufactured a prototype of appropriate elements which were evaluated in the preliminary test for selecting RFID tag, supply pump, mixing type, etc. With the prototype, the performance test for liquid feeding gilt was done in group raising training barn. Results: The performance test result shows the C.V.(coefficient of variation) of liquid mixing and feeding is 0.77∼1.97% and 2.4∼5.3%, respectively, which means the system could feed sows uniformly. The feeding time of pneumatically activated ball valve is 2 seconds per 0.9 kg of liquid feed. The eating time for gilt were 65 seconds in average. Conclusions: The prototype could feed sow uniformly, and deliver the liquid feed 9 times for a sow continuously. Also, total eating time for a sow was 18 minutes in one visit to the prototype of feeding station.