세계의 명문대학(4)-하바드 대학

김용덕,Kim, Yong-Deok 한국대학교육협의회 1983 大學敎育 Vol.5 No.-

부산광역시 신청사 건립공사 현장

김용덕,Kim, Yong-Deok 한국건설안전협회 1996 建設 安全技術 Vol.19 No.-

고출력 무선국간의 수동 상호변조시 특성분석에 대한 연구

김용덕,조학현,Kim Yung-Duk,Jo Hak-Hyun 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.1

스퓨리어스의 측정은 안테나 급전선에서 측정하는데, 급전선후단에서 발생되는 수동상호변조에 대한 측정안이 마련되어야 함이 요구된다. 인접한 주파수대역의 무선국간 수동상호변조에 대한 특성분석과 실험을 통하여 수동상호변조 발생가능성을 알아 보았다. 수동상호변조에 대한 피해를 방지하고자 본 논문에서는 방사된 저파의 스퓨리어스 측정안을 마련함을 목적으로 한다. As spurious emission strength is normally measured at the antenna feeder, It is required that the measurement method on passive Intermodulation occurred on the latter part of antenna feeder be made. through the tests and the analyses of passive Intermodulation between radiostations of adjacent frequency bands, the possibility of occurrence of passive Intermodulation was testified in this study. The purpose of this study is to propose the measurement method of spurious emission strength in order to prevent the bad effect by passive Intermodulation.

기계적합금화한 AI-Ti합금의 고온인장특성에 미치는 Zr의 첨가와 등온열처리의 영향

김용덕,원형민,김선진,Kim, Yong-Deok,Won, Hyeong-Min,Kim, Seon-Jin 한국재료학회 1996 한국재료학회지 Vol.6 No.11

Ai-8wt.5(Ti+Zr)합금을 기계적합금화와 열간압출로 제조하여 Ti에 대한 Zr 첨가비와 등온열처리가 고온인장강도 및 변형거동에 미치는 영향에 대하여 조사하였다. Ti에 대한 Zr 첨가량의 비가 증가함에 따라 열간압출 시편의 상온 및 고온강도가 증가하였고, 40$0^{\circ}C$ 및 51$0^{\circ}C$에서 등온열처리에 따른 강도의 감소도 작게 나타났다. 이는 Zr 첨가량이 증가함에 따라 AI 기지와 AI3Ti에 비해 작은 격자간불일치도를 갖는 AI3(Ti+Zr)금속간화합물이 생성되고 고온열처리에 따른 조대화가 억제되었기 때문으로 판단되었다. 합금의 연성은 Zr 첨가량과 등온열처리에 관계없이 10% 이하로 낮게 나타났으며 인장 시험 온도가 고온일수록 취성파괴인 입계파괴가 지배적으로 일어났다. AI-Ti-Zr 합금의 변형에 필요한 활성화에너지는 순수한 AI 기지의 자기확산에 필요한 활성화에너지 142KJ/mol에 비해 573-783K 온도범위에서 1.5-1.8배 높은 값을 보였으며, Ti에 대한 Zr의 첨가량의 비가 증가할수록 보다 높은 값을 나타내었다.

쥐의 간 Cytosolic Aldehyde Reductase의 정제 및 특성에 관한 연구

김용덕,주충노,Kim, Yong-Duk,Joo, Chung-No 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6

쥐의 간 시토졸 분획에서 두 가지 aldehyde reductase [ALR: EC 1.1.1.2J를 분리하였는데, 그 중 p-nitrobenzaldehyde에 대한 $K_m$값이 높은것(high $K_m$, ALR)은 황산암모늄 분별침전, DEAE-Sephacel, hydroxyapatite, blue sepharose CL-6B chromatography 방법을 이용하여 분리 정제하였고, $K_m$값이 낮은것(low $K_m$ ALR)은 활성이 적고 불안정하여 부분 정제하여 특성을 조사하였다. High $K_m$ ALR은 SDS-PAGE상에서 42,000 dalton의 단일띠로 나타났으며 superose 12 column을 이용한 FPLC gel filtration을 수행한 결과 분자량이 44,000 dalton의 monomer임이 확인되었다. High $K_m$, ALR의 최적 pH는 6.5, pI는 6.2이었고 low $K_m$ ALR의 최적 pH는 5.5이었다. 두 ALR 모두 $37^{\circ}C$ 에서 안정하였으나 $54^{\circ}C$ 이상에서는 활성이 급격히 저하되었다. High $K_m$, ALR. low $K_m$ ALR은 모두 p-nitrobenzaldehyde에 대해 좋은 반응성을 가쳤고 특히 high $K_m$ ALR은 succinic semialdehyde에 대해 높은 반응성을 나타낸 것이 특색이다. 그러나 high $K_m$ ALR과 low $K_m$, ALR의 기질 특이성에는 상당한 차이가 있었다. High $K_m$, ALR은 보조효소로 NADPH만을 이용하지만 low $K_m$, ALR은 NADH, NADPH를 모두 보조효소로 이용한다. 두 효소는 $Mg^{2+}$, $Li^+$, $Zn^{2+}$, $Ca^{2+}$에 의한 영향을 받지 않았으나, high $K_m$, ALR은 $Cu^{2+}$에 의해 활성이 억제되었는데, low $K_m$, ALR은 반대로 $Cu^{2+}$에 의해 활성이 촉진되었다. Barbital은 high $K_m$, ALR에 대해 비경쟁적 억제작용을 나타내고 $K_i$값은 $35{\mu}M$이였으며, indole-3-acetate에 의해서는 반경쟁적인 억제현상을 나타내고 $K_i$값은 $496{\mu}M$이었다. Aldehyde reductase (ALR) isozymes of rat liver cytosol fraction were separated and the ALR having high $K_m$ $(101{\mu}M)$ for p-nitrobenzaldehyde was purified by the methods of ammonium sulfate precipitation, DEAE-Sephacel chromatography, hydroxyapatite chromatography and Blue-Sepharose CL-6B affinity chromatography. The ALR having low $K_m$ $(29{\mu}M)$ for p-nitrobenzaldehyde was only partially purified because of its instability and low activity. The molecular weight of high $K_m$ ALR was determined to be 44,000 dalton by Superose gel filtration in FPLC system. SDS-polyacrylamide gel electrophoresis showed that it was monomer having molecular weight of 42,000 dalton. The optimal pH of high $K_m$ ALR was 6.5 and that of low $K_m$ ALR was 5.5. The isoelecric point of high $K_m$ ALR was 6.2. The above two isozymes were very stable at $37^{\circ}C$, but their activities decreased rapidly as the temperature increased to $54^{\circ}C$. The good substrate of the above two isozymes was found to be p-nitrobenzaldehyde, but their substrate specificity was very different from each other. For low $K_m$ ALR, both NADH and NADPH were used as an coenzyme but high $K_m$ ALR used only NADPH as a coenzyme. It was also realized that $Cu^{2+}$ inhibited high $K_m$ ALR activity but stimulated low $K_m$ ALR activity. Barbital was shown to act as noncompetitive inhibitor of high $K_m$ ALR and its $K_i$ was $35{\mu}M$. Indole-3-acetate was shown to act as uncompetitive inhibitor of high $K_m$ ALR and its $K_i$ was $496{\mu}M$.

제22회 한국원자력기술상 수상기 - 원전연료의 생노병사를 연구하며 꿈꾸는 미래

김용덕,Kim, Yong-Deok 한국원자력산업회의 2015 원자력산업 Vol.35 No.3

그리스 차량 판매공사의 문제 해결 사례

김용덕,Kim, Yong-Deok 한국정보통신집흥협회 1998 정보화사회 Vol.123 No.-

쥐의 간 Cytosolic aldehyde Reductase 의 정제 및 특성에 관한 연구

김용덕,주충노 ( Yong Duk Kim,Chung No Joo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6

Aldehyde reductase (ALR) isozymes of rat liver cytosol fraction were separated and the ALR having high K_m (101 μM) for p-nitrobenzaldehyde was purified by the methods of ammonium sulfate precipitation, DEAE-Sephacel chromatography, hydroxyapatite chromatography and Blue-Sepharose CL-6B affinity chromatography. The ALR having low K_m (29 μM) for p-nitrobenzaldehyde was only partially purified because of its instability and low activity. The molecular weight of high K_m ALR was determined to be 44,000 dalton by Superose gel filtration in FPLC system. SDS-polyacrylamide gel electrophoresis showed that it was monomer having molecular weight of 42,000 dalton. The optimal pH of high K_m ALR was 6.5 and that of low K_m ALR was 5.5. The isoelecric point of high K_m ALR was 6.2. The above two isozymes were very stable at 37℃, but their activities decreased rapidly as the temperature increased to 54℃. The good substrate of the above two isozymes was found to be p-nitrobenzaldehyde, but their substrate specificity was very different from each other. For low K_m ALR, both NADH and NADPH were used as an coenzyme but high K_m ALR used only NADPH as a coenzyme. It was also realized that Cu^(2+) inhibited high K_m ALR activity but stimulated low K_m ALR activity. Barbital was shown to act as noncompetitive inhibitor of high K_m ALR and its K_i was 35 μM. Indole-3-acetate was shown to act as uncompetitive inhibitor of high K_m ALR and its K_i was 496 μM.

인간 무세포성 진피기질 위에 배양한 가토 구강각화상피세포의 중충화와 기저막 형성에 관한 연구

김용덕,안강민,염학렬,정헌종,김성민,장정원,성미애,박희정,황순정,이종호,Kim, Yong-Deok,Ahn, Kang-Min,Yum, Hak-Yeol,Chung, Hun-Jong,Kim, Soung-Min,Jang, Jeong-Won,Sung, Mi-Ae,Park, Hee-Jung,Hwang, Soon-Jung,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.6

To assess the clinical applicability of bio-artificial mucosa which was made with autologous oral keratinocytes and human acellular dermal matrix, the formation of basement membrane and stratification of oral keratinocytes were evaluated. Six New Zealand white rabbits (around 2kg in weight) were anesthetized and its buccal mucosa was harvested (1.0 $\times$ 0.5cm size). Oral keratinicytes were extracted and cultured primarily with the feeder layer of pretreated NIH J2 3T3 fibroblast. These confluent cells were innoculated on the human acellular dermal matrix and cultured in multiple layer by air-rafting method. After 3, 5, 7, 10, 14 days of culture, each cultured bio-artificial mucosa was investigated the number of epthelial layer of by H&E stain and toluidine blue stain. The immuhohistochemical methods were used to evaluate the cell division capacity, the formation of basement membrane, and it's property of specific cells (PCNA, cytokeratin 14, laminin). Transmission electromicroscopy was used for the attachment between cells and matrix with the number of hemidesmosome. In result, the numbers of layer of stratified growth of oral keratinocyte cultured on the human acellular dermal matrix and the number of hemidesomal attachment between epithelial cells and human acellular dermal matrix were similar to the layers of normal oral mucosa after 10 days of culture. The cell division rate, basement membrane formation and proliferation rate increased as culture period increased. With these results, bio-artificial mucosa with autologous oral epithelial cells cultured on the acellular dermal matrix had clinically adaptable properties after 10 days' culture and this new bio-artificial mucosa model with relatively short culture time can be expected clinical applicability.

95년 결산-경영혁신 바람부는 한화에너지

김용덕,Kim, Yong-Deok 대한석유협회 1995 석유와 에너지 Vol.1995 No.12