DEAE - Cellulose 에 의한 casein 의 분별에 관한 검토

김영교,신동철,전우민 한국낙농학회 1983 韓國酪農學會誌 Vol.5 No.3

This paper is described on the method for fractionation of casein mixtures by the DEAE-Cellulose column chromatography. For the fractionation of casein mixtures, the method used was ion exchange chromatography on the DEAE-Celluose, Whatman DE52, with tris-chloride-urea buffer (PH 8.6), and a NaCl gradient. The effects of the column length, NaCl concentration and alkylation of the sample were tested for the fractionation of casein mixtures by DEAE-Celluose column chromatography. The results were summarized as follows; 1. By the long column (1.5 × 50 ㎝), γ-casein was fractionated into two peaks, and by the short column (1.5 × 25 ㎝), γ-casein was eluted with a single peak. 2. When the sample was applied to the column and eluted with about 100 ㎖ of the buffer (above bed volumn) containing 0.03 M NaCl, the pure γ-casein was isolated without the contamination of κ-casein. 3. γ-Casein, κ-casein, β-casein, and αs-casein were eluted at 0-0.03 M, 0.04-0.12 M, 0.13-0.15 M and 0.16-0.24 M NaCl concentration, respectively. 4. The fractionation of κ-casein and minor α-casein were improved by the alkylation of casein mixtures.

우유 단백질

김영교,Kim, Yong-Kyo 한국식품과학회 1972 한국식품과학회지 Vol.4 No.3

문학 : 시인론 ; 멸집(滅執)과 만공(滿空)의 시학 -시인 고영섭론-

김영교 한국불교사연구소 2006 문학/사학/철학 Vol.6 No.-

유도무기체계 운용시험평가시 M&S 적용방안에 대한 연구- 현궁사업 중심으로 -

김영교,강지훈 한국방위산업학회 2013 韓國防衛産業學會誌 Vol.20 No.1

Due to the high demand for more complex and technical weapon systems, the higher status of Test and Evaluation (T&E) organization has to develop highly objective, scientific and internationally recognized methods throughout the weapon system acquisition process. An example of this can be made of the guided weapon system, which has intellectual and complex functions and can easily become a high-cost project. The military in advanced nations adopt Modeling and Simulation (M&S) in order to minimize Research and Development (R&D) cost, incertitude and risk. The USA has been using both M&S and VV&A (Verification, Validation, and Accreditation) in order to maintain regulations, directives and manuals. In South Korea, however, there are currently no systems to facilitate the application of M&S. In this study, I have tried generating several test items wherein M&S can be applied to ongoing projects of infantry middle range guided weapon (Hyun-gung) and I also suggested a proper M&S method that is currently being applied. Among the M&S methods is the operational effective analysis model, which has been used by the US Army OT (Operational Test). It is considered 'suitable' to measure operational effectiveness and it is a necessary element for the future operational T&E. The procedure of VV&A, which is the key to obtaining credibility, should also be applied to other weapon system's R&D. It will be very beneficial to the increase of the domestic T&E system level.

Effects of ibuprofen-loaded TiO2 nanotube dental implants in alloxan-induced diabetic rabbits

김영교,김완태,정보현,유기연,엄흥식,장범석,이재관,최원열 대한치주과학회 2021 Journal of Periodontal & Implant Science Vol.51 No.5

Purpose: Some systemic conditions, especially diabetes mellitus (DM), adversely affect dental implant success. This study aimed to investigate the effects of ibuprofen-loaded TiO2 nanotube (ILTN) dental implants in alloxan-induced diabetic rabbits. Methods: Twenty-six New Zealand white rabbits were treated with alloxan monohydrate to induce DM. At 2 weeks following DM induction, 3 types of implants (sandblasted, large-grit, and acid-etched [SLA], ILTN, and machined) were placed into the proximal tibia in the 10 rabbits that survived following DM induction. Each type of implant was fitted randomly in 1 of the holes (round-robin method). The animals were administered alizarin (at 3 weeks) and calcein (at 6 weeks) as fluorescent bone markers, and were sacrificed at 8 weeks for radiographic and histomorphometric analyses. Results: TiO2 nanotube arrays of ~70 nm in diameter and ~17 μm in thickness were obtained, and ibuprofen was loaded into the TiO2 nanotube arrays. A total of 26 rabbits were treated with alloxan monohydrate and only 10 rabbits survived. The 10 surviving rabbits showed a blood glucose level of 300 mg/dL or higher, and the implants were placed in these diabetic rabbits. The implant stability quotient (ISQ) and bone-to-implant contact (BIC) values were significantly higher in the ILTN group (ISQ: 61.8, BIC: 41.3%) and SLA group (ISQ: 62.6, BIC: 46.3%) than in the machined group (ISQ: 53.4, BIC: 20.2%), but the difference in the BIC percentage between the SLA and ILTN groups was not statistically significant (P=0.628). However, the bone area percentage was significantly higher in the ILTN group (78.0%) than in the SLA group (52.1%; P=0.000). Conclusions: The: ILTN dental implants showed better stability (ISQ) and BIC than the machined implants; however, these values were similar to the commercially used SLA implants in the 2-week diabetic rabbit model.

아이스크림의 種類와 品質

金榮敎 한국식품과학회 1974 식품과학과 산업 Vol.7 No.3

Mucor miehei Protease 의 이화학적 성질에 관한 연구

김영교,문용일,박동준 한국낙농학회 1984 韓國酪農學會誌 Vol.6 No.2

本 實驗은 casein, 脫脂乳 그리고 유청단백질 등의 기질에 대한 MM protease의 기초적인 理化學的 성질을 파악하기 위하여 NPN定量, 電氣泳動 그리고 凝乳時間測定 등을 실시하였다. 그 결과를 요약하면 다음과 같다. 1. Casein용액(2%)에서 MM protease의 最適 pH는 6.25로 나타났으며, 알칼리상태에서는 급격히 活性이 저하되었다. 2. Casein용액(2%)에서 MM Protease의 最適溫度는 45℃였으며, 60℃까지 완만하게 活性이 떨어지다가 70℃이상에서는 급격히 活性이 저하되었다. 3. 반응시간의 경과에 따라 rennet과는 달리 MM protease는 유리되는 NPN量이 계속 증가하였는데, 초기에는 급속한 증가를 보이다가 2時間 반응 후로는 완만해졌다. 4. 脫脂乳에 CaCl₂를 0.002∼0.01%범위에서 첨가하였을 때, 농도의 증가에 따라서 응고시간도 점점 빨라졌으며, MgSO₄를 0.2∼1.0%범위로 첨가하였을 때에도 응고시간이 발라졌다. 2%이상의 NaCl과 0.002%이상의 CuSO₄의 첨가는 농도가 증가함에 따라 응고를 점점 더 지연시켰다. 5. 유청단백질에 대한 MM protease의 作用을 조사한 결과 유리되는 NPN量은 반응시간의 증가에 따라서 증가하였으나 전기영동상으로는 그 분해받은 정도를 알 수 없었다. 6. Casein용액(2%)에 MM protease와 rennet를 각각 반응시킨 결과 α_s-casein은 MM protease에 의해서 rennet보다 더 분해를 받았으며, β-, κ-casein은 rennet과 거의 비슷한 경향을 나타냈다. 7. MM protease를 전기영동한 결과 κ-casein과 β-casein사이에 단일 band로 나타났다. 8. α_s-casein은 MM protease에 의하여 분해되어 이동도가 빨라졌고, β-casein은 약간 느려졌다. κ-casein은 rennet과 마찬가지로 para-κ-casein의 위치에 단일 band로 나타났다. This experiment was carried out to study the proteolytic activities of Mucor miehei protease by means of polyacrylamide gel electrophoresis, clotting test and quantitative analysis of NPN. The results were summarized as follows: 1. The optimum pH of MM protease activity on 2% casein solution was pH 6.25 and the activity was markedly declined in alkaline phase. 2. The optimum temperature of MM protease activity on proteolysis of 2% casein solution was 45℃ and gradually declined from 45℃ to 65℃ and markedly inactivated above 70℃. 3. The liberation of NPN from 2% casein solution was increased during the 4 hours of incubation. But after the 2 hours of incubation, the increasing rate was lowered. 4. The clotting time of skimmilk was curtailed when the skimmilk contained 0.002 to 0.01% CaCl₂and 0.2 to 0.1% MgSO₄. Conversely, the clotting time was delayed when 2 to 10% NaCl and 0.002 to 0.01% CuSO₄was added. 5. The liberation of NPN from whey protein was increased with incubation time, but the electrophoretic patterns of whey protein before and after the action of MM protease were almost identical. 6. On electrophoresis of the 2% casein solution treated with MM protease, α_χ-casein's degradation was greater than rennet, but the hydrolysis of β- and κ-casein were similar to that of rennet. 7. MM protease was shown as a single band on electrophoresis, and the mobility of MM protease was between β-casein and κ-casein. 8. On electrophoresis of α_s-casein, β-casein and κ-casein treated with MM protease, α_s-casein moved faster than that of original band and β-casein's mobility was lowered, and for κ-casein, it was completely converted into para-κ-casein which moved toward the cathod.

린드레스치이스의 이점과 문제점

김영교 한국식품산업협회 1978 좋은식품 Vol.43 No.-

탈지분유의 이화학적 성질에 관한 연구

김영교,최장현 한국낙농학회 1994 韓國酪農學會誌 Vol.16 No.3

본 실험은 국내산과 수입되고 있는 탈지분유의 성분규격 및 이화학적인 성질을 비교, 조사하기 위하여 수행하였다. 일반성분은 비교적 표준규격에 적합한 것으로 드러났다. pH는 제품마다 차이를 보였고 산도는 표준규격에 적합 하였다. 가밀도를 고려할시 국내제품의 입자가 더 큰 것을 알 수 있었고, 용해도와 분산성은 대부분의 제품에서 유의적인 차이를 보였다 (P<0.05). NCN은 국내제품이 일반적으로 더 높았으나 NPN에서는 유의적인 차이가 나타나지 않았다(P<0.05). NCN과 WPNI로 국내,외 제조회사들의 다양한 열처리 공정을 추정할 수 있었고 전기영동상의 유청단백질의 변성도도 이와 유사한 경향을 보였다. Chymosin 작용에 의한 응고시간은 전 제품에서 차이를 보였으며 생유보다 지연됨을 알 수 있었고, 이때 유리되는 NPN 역시 생유보다 적게 나타났다. HMF 함량은 7.78∼11.07μ㏖/ℓ로 나타났으며 국내제품과 수입품들간에 큰 차이는 나지 않았다. TTC test 항생물질 검출한도내로는 이들 물질들이 존재하지 않는 것으로 판정되었다. To compare the qualities of domestic and foreign dried skim milk, their physico-chemical properties were determined. The contents of total solids, water, fat, protein, lactose and ash were in the range of 95.32∼97.07%, 3.02∼4.70%, 0.96∼1.18%, 31.57∼36.99%, 50.59∼55.81% and 7.11∼8.23%, respectively. Those values were met with the standard component regulation. In most products, the pH-values showed a little difference and were lower than that of fresh product. But titratable acidities were met with the standard regulation. Considering the bulk density, the particle sizes of domestic products were relatively larger than those of foreign products. The ranges of solubility index and dispersibility were 0.32∼0.77㎖ and 41.16∼94.80%. Among the products, there was a significant difference(P<0.05) in the above two factors. Generally the NCN of domestic products were higher than those of foreign products, but in the case of NPN, no significant difference was shown in all products(P<0.05). The electrophoretic patterns of whey protein of some products were different from those of raw milk. That is, the densities of β-lactoglobulin and serum albumin bands were lower than those of raw milk. The undenatured whey protein nitrogen index(WPNI) contents showed 2.14-8.02 ㎎/g, therefore, this indicated that heating condition to manufacture dried milk may be different between Korea and foreign countries. The coagulation time of all products by chymosin action was longer than that of raw milk and considerable difference was shown in all products. Compared with raw milk, NPN released by chymosin action was less in all products. The hydroxymethylfurfural(HMF) contents showed 7.78∼11.07 ㏖/ℓ and the difference between domestic and foreign products was little. The contents of antibiotic substances did not exceed the maximum allowance amount by TTC test in all products.

원예공로상

김영교 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10