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건식제련용 동 함유 슬러지 펠렛 제조 및 물리적 특성평가
김수윤,김영진,김승현,이재령,Kim, Suyun,Kim, Youngjin,Kim, Seunghyun,Lee, Jaeryeong 한국자원리싸이클링학회 2019 資源 리싸이클링 Vol.28 No.2
The pelletizing of printed circuit board (PCB) sludge was researched for copper recovery in pyrometallurgical process. This pelletizing was carried out by using self-manufactured compression-type apparatus after pre-treatments (drying, water scrubbing, size classification) were proceeded. The physical properties (compression strength and drop-breakage test) were tested with a change of sludge sizing and the number of compression. In the case of using the undersized sludge of #140, its properties were improved to 0.6 MPa and 9.3 times. Moreover, they increased to 0.82 MPa and 19.0 times by using the #140 ~ 325 sludge. These imply that the packing density increases due to the elimination of large-sized sludge (#140), and also the weight of required binder decreases by the removal of fine-sized sludge (#325).
연구보문 : BrFLC 유전자를 이용한 만추대 계통 배추의 추대지연
김수윤 ( Soo Yun Kim ),김정선 ( Jung Sun Kim ),김진아 ( Jin A Kim ),박범석 ( Beom Seok Park ),홍준기 ( Joon Ki Hong ),박영두 ( Young Doo Park ),이연희 ( Yeon Hee Lee ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.2
배추 순계 계통의 재분화율을 서울배추와 비교하여 분석한 결과 캘러스 상태와 신초발생율이 높아 형질전환 재료로 적합하였다. 재분화된 신초는 1/2MS 배지보다는 Hyponex 배지에서 발근이 잘 되었으며 식물체 생장도 좋았다. 배추 순계 계 통에 배추로부터 분리한 BrFLC1과 BrFLC3 유전자를 각각 과발현 벡터를 이용하여 형질전환하였다. 하이그로마이신 배지에서 선발한 형질전환체를 T1, T2 세대별로 추대 시기를 분석한 결과 비형질전환체보다 추대 시기가 30일 이상 늦어지는 계통을 선발할 수 있었으며 선발된 계통 내에 있는 개체들 사이에서의 추대 시기도 모두 지연되었다. Brassica rapa L. ssp. pekinensis inbred line showing a very high frequency of shoot regeneration was used for transformation with Agrobacterium tumefaciens GV3101 harboring genes for hygromycin resistance (hpt) and floral repressor, BrFLC1 and BrFLC3 genes. Induction and development of roots from regenerated shoots were more effective on HYP medium containing Hyponex powder (3 gL-1) than half strength MS medium. The BrFLC1 and BrFLC3-expressed transgenic plants showed delayed bolting time through T1 and T2 generation, indicating that control of floral transition time could be possible in Brassica crops.
에델바이스캘러스배양추출물의 항노화 관련 효능에 관한 연구
김수윤(Soo-Yun Kim),김혜인(Hye-In Kim),류승환(Seung-Hwan Ryu),박정곤(Jeong-Gon Park),조선영(Seon-Young Jo),김주덕(Ju-Duck Kim),모상현(Sang-Hyun Moh) 한국화장품미용학회 2018 한국화장품미용학회지 Vol.8 No.2
Edelweiss, which belongs to the Asteraceae family, has long been used in a variety of medicinal remedies against abdominal, respiratory, inflammation and fever diseases. It is well-known Switzerland national flower and be protected species in many countries. In order to preserve and utilize endangered edelweiss, its callus was derived from seeds using plant tissue culture technology. Edelweiss callus extract (Leontopodium Alpinum Callus Culture Extract; LACCE) was evaluated for its anti-aging effect in the skin cells. The cytotoxicity of the LACCE was examined on HaCaT and Ditroit 551 cells with MTT assay. Anti-oxidant activity of LACCE was verified by In vitro test. The expression levels of MMP-2 and AQP-3 were analyzed on Detroit 551 and HaCaT cells by Real-time PCR to examine wrinkle effect and moisturizing effect. LACCE treated HaCaT cell shows increasing of AQP-3 mRNA levels and decreasing of MMP-2 mRNA levels. The anti-inflammatory effect of LACCE was estimated by the mRNA levels of COX-2 and iNOS in UVB treated HaCaT cells. COX-2 and iNOS mRNA levels were reduced in LACCE treated HaCaT cells. These results showed LACCE can be used in the promising anti-aging cosmetic active ingredients.
향기 나는 장미 (원교 D1-409) 캘러스 추출물의 항산화 및 항염, 보습 효과
김수윤(Soo-Yun Kim),백승혜(Seung-Hye Paek),이수영(Su-Young Lee),송지혁(Ji-Hyeok Song),김지연(Ji Yeon Kim),이정훈(Jeong Hun Lee) 한국화장품미용학회 2022 한국화장품미용학회지 Vol.12 No.2
Since ancient times, roses were grown for many different reasons as cut flowers, garden plants, and pot plants as well as for industrial (perfume), medicinal, and culinary applications. Wongyo D1-409 was cultivated and bred in the National Institute of Horticultural and Herbal Science. The embryogenic callus derived from the roses root was induced by using plant tissue culture technology. Then, the callus was extracted with water for the efficacy test. In Analyzes using HPLC, various minor peaks were detected in the extracts. As a result, ellagic acid and gallic acid was identified as the characteristic peak. After extraction of callus extract, biological evaluation including anti-oxidant, anti-inflammation, and moisturizing effect was performed. As a result, the callus extract showed remarkable biological activities through stimulating synthesis of super oxide dismutase(SOD1), catalase(CAT), nuclear respiratory factor 1(NRF1) and aquaporin(AQP3) expression, and inhibiting cyclooxygenase-2(COX-2) expression. Therefore, it is expected that the domestic rose-derived embryonic callus extract can be used in various ways for anti-aging cosmetic materials.
배추 작물에 이원적 전사유도 시스템 도입을 위한 조기 검증방법 확립
김수윤(Soo-Yun Kim),유희주(Hee-Ju Yu),김정호(Jeong-Ho Kim),조명철(Myeong-Cheoul Cho),박미희(Mehea Park) 한국원예학회 2013 원예과학기술지 Vol.31 No.1
이원적 전사유도 시스템(binary trans-activation system)은 도입유전자의 발현을 조절하는 기작(mechanism) 중에 하나로, 목적 유전자의 발현이 전사활성 인자를 가지고 있는 식물체와의 교배를 통해서만 발현되는 시스템이다. 본 연구에서는 이원적 전사유도 시스템을 원예 작물의 우수한 유전자원 및 신품종 보호 방법으로 이용하고자, 배추에서 이 시스템의 기능을 검정하였다. 배추작물에서 이원적 전사유도 시스템의 이용가능성을 검정하기 위하여 activator construct(35SLhGBart)와 reporter construct(pOpGUS1300)를 작성하였고 공동형질전환방법으로 배추에 형질전환하였다. 두 종류의 카세트가 도입된 형질전환체는 항생제를 이용하여 선발하였으며, 재분화된 신초의 GUS 유전자 발현으로 이 시스템의 활성을 확인하였다. 또한 이 시스템을 조직 특이적으로 유도하기 위하여 애기장대의 자성 배우체 특이적 프로모터를 이용하여 activator construct(795LhGBart)를 작성하여 애기장대에 형질전환 하였다. 공동형질전환된 애기장대는 자성 배우체에서 조직 특이적인 발현을 나타냈다. 이러한 결과는 이원적 전사유도 시스템이 목적유전자의 발현을 배추의 F₁ 종자에서 선택적으로 유도하는 방법으로써 우수한 유전자원 및 신품종 보호에 이용될 수 있다는 것을 보여주는 것이라고 생각된다. Binary trans-activation (pOp/LhG4) system is one of the regulatory systems of transgene expression. The target gene expression is achieved by crossing the reporter plants with an activator in this system. In this study, we used the features of this system in Chinese cabbage as a way to protect genetic resources and new varieties. To establish pOp/LhG4 system in Chinese cabbage, we designed an activator (35SLhG41300), and reporter constructs (pOpGUSBart) and co-transformed using Agrobacterium. The transgenic plants were selected by antibiotics and the functional activity of pOp/LhG4 system was confirmed by GUS expression. To induce the tissue-specific function, we constructed pOp/LhG4 system (795LhGBart) using female tissue specific promoter (ProAt1g26795) of Arabidopsis. Co-transformed transgenic plants clearly showed tissue specific expression in Arabidopsis. The results suggest the possibility of the system’s application of F₁ generation can be restricted by expressing the target gene to protect a new varety and genetic resource in Chinese cabbages.
자기조립단층과 농축 기술을 이용한 저농도 내분비계 장애물질 검출용 미소유체채널 기반 전기화학 센서
김수윤(Suyun Kim),한지훈(Ji-Hoon Han),박정호(James Jungho Pak) 대한전기학회 2016 전기학회논문지 Vol.65 No.4
This paper demonstrates a microfluidic electrochemical sensor for detecting endocrine disruptor such as estradiol at a very low concentration by using preconcentration technique. In addition, self-assembled monolayer(SAM) was also employed on the working electrode of the electrochemical sensor in order to increase the estradiol capture efficiency of the sensor. SAM treatment on the working electrode enhanced the specific binding between the surface of the working electrode and the estradiol antibody. The estradiol antibody was applied on the working electrode at different concentrations(10, 20, 50, 100, 200 pg/ml) for observing the concentration dependency. The measured electrochemical redox current changed with the amount of the bound estradiol on the Au working electrode surface and the sensor can detect all the target material when the immobilized antibody amount is more than the estradiol amount in the water. The elecrochemical estradiol sensor without SAM treatment showed a low current of 7.79 nA, while the sensor treated with SAM resulted in 339 nA at 200 pg/ml, which is more than 40 fold higher output current. When combining the preconcentration technique and the SAM-treated electrode, the measured current became more than 100 fold higher than that of the sensor without neither SAM treatment nor preconcentration technique. The combination of these two techniques can would enable the proposed microfluidic electrochemical sensor to detect a very low concentration endocrine disruptor.