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복숭아 내한성에 따른 Dehydrin 함량 및 유전자 발현
김금선 ( Keum Sun Kim ),신현석 ( Hyun Suk Shin ),오영재 ( Young Jae Oh ),오성일 ( Sung-il Oh ),김민아 ( Min-ah Kim ),이석호 ( Seok-ho Lee ),김임수 ( Im Soo Kim ),김대일 ( Dae Il Kim ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.5
복숭아 네 품종 ‘오도로끼’, ‘가납암백도’, ‘진미’, ‘장호원황도’ 신초의 저온순화 및 탈순화 동안 시기별 내한성 변화는 전해질 누출률을 분석하여 나타냈다. 또한 내한성 결정 요인을 분석하고자 SDS-PAGE를 이용하여 dehydrin 함량 변화를 확인하였으며, 그와 관련된 유전자 발현 분석은 quantitativereal-time RT-PCR을 이용하여 수행하였다. 네 품종의 내한성은 2012년 12월까지 꾸준히 증가하였으며 그 후 2013년 4월까지 감소하였다. PpDhn1 유전자가 인코딩하는 60kDa의 dehydrin 단백질은 탈순화기(2013년 3-4월)에 비하여 저온순화기(2012년11월-2013년 1월) 동안 높은 축적이 확인되었다. PpDhn1 유전자와 PpDhn3 유전자 발현양상은 복숭아 네 품종에서 내한성 변화와 평행하게 나타난 반면, PpDhn2 유전자는 뚜렷한 시기별 패턴을 나타내지 않았다. Differences in cold hardiness were performed by relative electrolyte leakage (REL) analysis in the shoot of the four peach cultivars (P. persica cvs. Odoroki, Kanoiwa Hakuto, Jinmi and Janghowon Hwangdo), including Korean bred cultivars during cold acclimation and deacclimation. In addition, changes of dehydrin proteins of each cultivar were analyzed using a SDS-PAGE protein profiles and expressions of gene related dehydrin were determined using a quantitative real-time RT-PCR. Cold hardiness in four cultivars increased steadily in December 2012, and then decreased to April 2013. Our result indicated that a 60 kDa of dehydrin protein encoded by PpDhn1 gene during cold acclimation (from November 2012 to January 2013) accumulated to higher degrees compared with deacclimation (from March 2013 to April 2013) in all cultivars. Expression pattern of PpDhn1 and PpDhn3 of four cultivars paralleled fluctuations in cold hardiness, whereas expression of PpDhn2 did not indicate any specific pattern.
초등 학습자의 스토리텔링 말하기 능력 분석 - 6가지 단면 중심으로
김금선(Kim Keum-Sun),신동일(Shin Dong-Il) 한국외국어교육학회 2008 Foreign languages education Vol.15 No.1
The purpose of this study is to investigate the storytelling performance of Korean elementary school students by using the framework of narrative assessment profile (Bliss, McCabe, & Miranda, 1998). Story samples were collected from ten six-grade students during the English storytelling class. The English storytelling class consisted of mini lesson, storytelling activity, storytelling practice and self-assessment. Every class student was asked to practice storytelling in performing three tasks: picture book, family photo, and story stem. In this study, family photo storytelling samples were used to investigate. They were analyzed from six dimensions: topic maintenance, event sequencing, explicitness, referencing, conjunctive cohesion and fluency. From the diagnostic profile of strengths and weaknesses, five types of storytelling performance were presented. The implications for classroom practice and assessment were discussed.
에피소드 구성력과 수험자의 스토리텔링 과업수행능력 분석
신동일,김금선,김나희 새한영어영문학회 2007 새한영어영문학 Vol.49 No.4
The purpose of this study is to investigate how Korean learners of English construct their linear story grammar (Stein & Glenn 1979) in oral proficiency testing situations. A total number of 31 storytelling samples from two oral proficiency tests were analyzed: MATE (Multimedia-Assisted Test of English) and OPIc(Oral Proficiency Interview computer). The 15 excerpts came from MATE and 16 from OPIc. This study explored which components of story grammar(setting, initiating events, internal responses, attempts, direct consequences, reactions) appeared in each of three categorized proficiency levels (advanced, intermediate high and low, intermediate low). It was found that the performance difference of episode completeness was explicit between each level of oral proficiency. The higher their speaking proficiency was, the more complete episodes were found in their storytelling samples. It was also found that the test-takers of English speaking lacked the competence in describing characters' feeling and emotion in their narratives regardless of their proficiency levels. Issues for further research are discussed.