가용 시간을 이용한 역할 기반 위임 모델

김경자,장태무,Kim, Kyoung-Ja,Chang, Tae-Mu 한국정보처리학회 2007 정보처리학회논문지 C : 정보통신,정보보안 Vol.14 No.1

기존의 RBAC(Role Based Access Control) 모델들은 사용자간의 역할 위임이나 역할 분리를 운용하기에는 역부족이다. 이에 RBAC을 바탕으로 역할이나 권한을 다른 사용자에게 위임하는 모델인 RBDM(Roie Based Delegation Model)이 연구되고 있다. 반면에, 역할을 위임 받은자의 악의적인 남용을 막을 수 있는 방안이 필요하다. 본 논문은 역할의 위임으로 인하여 위임 받은자의 악의적인 도용을 막기 위한 모델로서 위임 역할에 대한 폐지 절차를 시간상으로 제한을 두고, 위임하는 역할에 대하여 원사용자의 권한을 그대로 유지함으로써 위임으로 인한 역할간 계층구조의 보안상 취약점을 보완하고자 한다. 즉, 역할을 위임하고자 할때에는 위임에 대한 유효 기간을 같이 부여하는 기법으로 위임된 역할의 사용 권한을 시간상으로 폐지할 수 있게 한다. 또한, 위임된 역할에 대해서는 원사용자의 역할에 대한 권한을 계속 보유할 수 있도록 함으로써 위임으로 인하여 역할에 대한 사용이 정당하지 않은 경우에는 언제든지 회수가 가능할 수 있게 하였다. 본 제안 기법인 T-RBDM (Time-out based RBDM)은 기존의 위임 모델인 RBDM0, RBDM1, PBDM과 비교 분석함으로 기존의 위임 모델에 비해 보안 측면에서 더 강인함을 보인다. The existing RBAC models are not sufficient for managing delegations or separation of roles. Researches have been done on RBDM(Role Based Delegation Model) that deal with delegating role or permission to other users. In this paper, we divide the delegated roles into two groups: periodic and temporary delegation roles. When a role is delegated, a time period is assigned together, which is used to revoke the permission of delegated role automatically. In our model, the role of monotonic delegation by an original user can be revoked at any time in case of malicious use by the delegated user. The contribution of our model is that the malicious use of delegated role can be prohibited and security vulnerability in the role hierarchy due to role delegations can be alleviated. The proposed model, T RBDM(Time out Based RBDM) is analyzed and compared with the conventional models, such as RBDM0, RBDM1 and PBDM. Our model shows an advantage over other models in terms of security robustness.

Effect of plasmid curing on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W

김경자,이재림,양용준,Kim, Kyoung-Ja,Lee, Jae-Rim,Yang, Yong-Joon The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3

Effect of plasmid curing of Acinetobacter sp. B-W on the production of siderophore from glutamic acid as both carbon and nitrogen sole sources was investigated. Plasmid cured mutant of strain B-W lost the ability to produce siderophore from glutamic acid at $28^{\circ}C$. Transformant E. coli $DH5{\alpha}$ harboring 20 kb plasmid, that was isolated from wild type of strain B-W produced siderophore from glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$, but, not at $36^{\circ}C$. Production of siderophore from glutamic acid by transformant E. coli $DH5{\alpha}$ was completely inhibited by $10{\mu}M\;FeCl_3$. In previous report, catechol nature of siderophore produced from glutamic acid by strain B-W was detected by Arnow test. The siderophore produced from glutamic acid by transformant E. coli $DH5{\alpha}$ was also catechol type. Rf value of siderophore produced from transformant E. coli $DH5{\alpha}$ grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32 in butanol-acetic acid-water (12:3:5) as developing solvent. Rf value of the siderophore was the same with that of wild type of strain B-W. Thus a single plasmid of 20 kb seemed to be involved in the production of siderophore from glutamic acid. 플라스미드가 제거된 Acinetobacter sp. B-W 균주의 돌연변이체를 글루탐산을 유일한 탄소 원과 질소원으로 함유한 배지에 $28^{\circ}C$에서 배양한 결과 글루탐산으로부터의 시드로포어 생산이 억제되었다. B-W 원 균주의 20 kb 플라스미드를 가진 형질 전환체 대장균 $DH5{\alpha}$는 같은 조건의 배지에서 시드로포어를 생산하는 것으로 조사되었다. 그러나 $36^{\circ}C$에서는 형질 전환체 대장균 $DH5{\alpha}$의 시드로포어 생산이 강하게 억제되었으며, 돌연변이체 B-W 균주는 $28^{\circ}C$에서와 마찬가지로 $36^{\circ}C$에서도 시드로포어를 생산하지 못하였다. 형질 전환체로부터 생산된 시드로포어의 종류는 원 균주 B-W와 마찬가지로 Arnow 시험 결과 카테콜 형으로 조사되었으며, $10{\mu}M\;FeCl_3$를 첨가한 배지에서는 시드로포어 생산이 완전히 억제되었다. 형질전환체로부터 생산된 시드로포어의 TLC상에서의 Rf값은 butanol-acetic acid-water (12:3:5) 용매상에서 0.32로 원 균주 B-W에서 생산된 시드로포어와 같았다. 위와 같은 실험 결과로 글루탐산으로부터 생산된 시드로포어의 생합성에 관여하는 유전자들이 20 kb 플라스미드 상에 있는 것으로 추정되었다.

Strepfomyces lincolnensis M-20 균주로 부터 분리, 정제된 L-Asparaginase의 열안정성과 단백 가수 분해 효소에 대한 저항성

김경자,Kim, Kyoung-Ja 대한약학회 2007 약학회지 Vol.51 No.3

Thermostable asparaginase was purified to homogeneity from mesophilic Strepfomyces lincolnensis M-20 by 30${\sim}$70% ammonium sulfate precipitation and asparagine-Sepharose CL 6B affinity column chromatography, The apparent molecular mass of L-asparaginase by SDS-PAGE was found to be 47 kDa, whereas by its mobility on Sephacryl S-300 column was around 180 kDa, indicating that the enzyme at the native stage acts as tetramer, The purified enzyme showed a single band on acrylamide gel electrophoresis. The optimum pH and temperature were pH 9.5 and 55${\circ}$C, respectively. Chemical modification experiments of purified asparagines implied the existence cystein residue located at or near active site. Purified asparaginase retained the 85% of the initial activity after incubation at 90${\circ}$C for 30 min. A correlation between themostability and resistance to proteolysis of commercial asparaginase and purified asparaginase from Strepfomyces lincolnensis M-20 was investigated. Purified thermostable asparaginase was resistant to trypsin and chymotrypsin treatment, while the commercial asparaginase was not themostable and was susceptible to proteolytic treatment with trypsin and chymotrypsin.

Burkholderia sp. CAS-5 균으로 부터 생산된 시드로포어의 Matrix metalloproteinase-2(Gelatinase A) 억제 활성

김경자,Kim, Kyoung-Ja 대한약학회 2006 약학회지 Vol.50 No.4

Matrix metalloproteinase-2 is known to be involved in pathological processes such as tumor invasion or rheumatoid arthritis. A soil microorganism producing siderophore under low iron stress $(up\;to\;5\;{\mu}m\;of\;iron)$ was identified as Burkholderia sp. Hydroxamate type siderophore produced by Burkholderia sp. CAS-5 was partially purified. MMP inhibitory activity of siderophore was confirmed by gelatin zymography. The $Zn^{2+}-chelating$ activity of siderophore correlated with the inhibition of MMP-2 activity.

Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W

김경자,장주호,양용준,Kim, Kyoung-Ja,Jang, Ju-Ho,Yang, Yong-Joon The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.2

포도당과 글루탐산을 함유한 배지에서 시드로포어인 2, 3-dihydroxybenzoic acid (DHB)를 생산하는 Acinetobacter sp. B-W 균주를 글루탐산을 유일한 탄소원과 질소원으로 함유한 배지에 배양한 결과, 상등액에서 2, 3-DHB가 아닌 카테콜 형의 시드로포어를 생산하는 것으로 조사되었다. 글루탐산의 농도는 3%에서 시드로포어 생산이 최고로 나타났으며, 3% 보다 높은 농도에서는 감소하는 것으로 조사되었다. 글루탐산을 유일한 탄소원과 질소원으로 함유한 배지에서 자란 균주 B-W는 배양 온도 $28^{\circ}C$에서는 시드로포어를 생산하지만 $36^{\circ}C$에서는 생산하지 않았다. 또한 $10{\mu}M\;FeCl_3$를첨가한 배지에서는 시드로포어 생산이 완전히 억제되었다. 글루탐산 배지에서 생산된 균주 B-W의 시드로포어는 TLC 전개 용매 butanol: acetic acid: water =12:3:5에서 Rf치가 0.32로 나타나 Rf치가 0.82인 2, 3-DHB와는 다른 것으로 조사되었으며, 또한 항산화 활성도 없는 것으로 나타나 항산화 활성을 가진 2, 3-DHB와는 다른 시드로포어인 것으로 추정되었다. Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at $36^{\circ}C$, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$.

Paleohydrologic Activity and Environmental Change on Mars

김경자,Dohm, James M.,Kim, Kyeong-Ja 한국제4기학회 2009 제사기학회지 Vol.23 No.1

Results from the most recent decade of Mars' missions to Mars highlight a liquid water and water-ice sculpted landscape. Evidence includes layered sedimentary sequences with weathered outcrops, debris flows, fluvial valleys, alluvial fans, deltas, glacial and periglacial landscapes, and geochemical/mineralogical signatures of aqueous activity, including the formation of sulfates and clays, and the leaching and deposition of elements such as potassium, thorium,and iron. Such evidence indicates weathered zones and possible paleosols in stratigraphic sequences, transport of water and rock materials to sedimentary basins, and the possible formation of extensive transient lakes and possibly transient oceans on Mars. This new evidence is consistent with Viking-era geologic investigations that reported magmatic-driven flooding, ponding to form large water bodies in the northern plains, and transient (tens of thousand of years) hydrological cycles. It may even indicate aqueous activity at present. Both endogenic (magmatic driven) and exogenic (both impact cratering and changes in orbital parameters) have influenced paleohydrologic and environmental change on Mars. Abundance of water and dynamic activity would be decisively important for the possibility of past and present life on Mars.

장기요양시설 요양보호사의 감염관리 수행에 영향을 미치는 요인

김경자,박성원,Kim, Kyoung Ja,Park, Sung Won 한국가정간호학회 2016 가정간호학회지 Vol.23 No.2

Purpose: The purpose of this study was to investigate factors influencing health workers' infection management behavior in long-term care facilities. Methods: A descriptive cross-sectional survey was conducted with 180 health workers who are employed in long-term care facilities. The data were collected from April, 25 until July, in 2016. Results: Infection management behavior positively correlated with the perceived importance of infection management (r=.77, p<.001), but role conflict negatively correlated with infection management behavior (r=.28, p<.001). The hierarchical regression model with general characteristics (first step) and perceived importance of infection management, work environment, and role conflict (second step) against infection management behavior was statistically significant (F=31.93, p<.001). This model could explain 62.8% of infection management behavior ($R^2=.62$, ${\Delta}R^2=.39$). Particularly, perceived importance of infection management was identified as factors influencing infection management behavior(${\beta}=.70$, p<.001). Conclusion: The findings of this study imply that systemic education about infection control and monitoring should be considered, so as to encourage proper infection management behaviors among health workers in long-term care facilities.

장반감기 우주선유발 동위원소, $^{10}Be$과 그 응용

김경자,우형주,Kim, K.J.,Jull, A.J.T,Woo, H.J. 한국제4기학회 2006 제사기학회지 Vol.20 No.2

반감기가 긴 $^{10}Be$ (Beryllium-10)은 지구의 대기뿐만 아니라, 지구의 표면 그리고 다른 행성의 대기와 표면에서도 생성된다. 가속기질량분석기술(AMS)이 1970년 후반기에 개발되면서 지질분야 연구에서 매우 중요한 추적자 중의 하나인 $^{10}Be$ 측정이 가능해졌으며, 이후 지구와 지구외 시료를 이용한 다양한 $^{10}Be$ 응용연구가 방사성탄소 응용연구와 함께 이루어져 왔다. 본 논문은 지구와 행성의 대기와 표면에서의 $^{10}Be$ 생성에 관한 메카니즘과 지형학, 해양학, 고고학, 빙하학, 우주방사화학, 기후학, 그리고 행성학 등의 여러 학문 분야에서의 $^{10}Be$의 응용연구들에 대하여 정리하였으며, 아울러 $^{10}Be$ 가속기질량분석을 이용한 향후 연구과제들을 고찰하였다. The long-lived radionuclide, $^{10}Be$, is produced by cosmic-ray effects in the atmosphere of the earth as well as its surface and that of other planetary surfaces and atmospheres. Accelerator mass spectrometry (AMS) was developed in late 1970s, which made $^{10}Be$ terrestrial measurements more feasible. Since then, many research applications of $^{10}Be$ for both terrestrial and extraterrestrial applications have been developed, which parallel the wide range of radiocarbon ($^{14}C$) research applications. This paper summarizes production mechanisms of $^{10}Be$ both in the atmosphere and on the surface of terrestrial and extraterrestrial environments and also provides numerous $^{10}Be$ research applications in the fields of geomorphology, oceanography, archaeology, glaciology, cosmochemistry, climatology, and planetary science. We also review some $^{10}Be$ AMS research applications.

Effect of plasmid curing on the 2, 3-dihydroxybenzoic acid production and antibiotic resistance of Acinetobacter sp. B-W

김경자,김진우,양용준,Kim, Kyoung-Ja,Kim, Jin-Woo,Yang, Yong-Joon The Microbiological Society of Korea 2016 미생물학회지 Vol.52 No.3

시드로포어인 2, 3-dihydroxybenzoic acid (DHB)를 생산하는 Acinetobacter sp. B-W의 플라스미드를 분석한 결과, 20 kb 크기의 플라스미드를 함유하였다. 배양 온도 $43^{\circ}C$ 가 플라스미드가 제거된 돌연변이체 생산에 효과적이었다. 이 돌연변이체는 2,3-DHB 생산 능력을 소실하였으며, chrome azurol S (CAS) 아가 배지에서 시드로포어 생산이 검출되지 않았다. 포도당과 황산 망간을 함유한 배지에서 $28^{\circ}C$로 3일간 배양한 B-W 원 균주와 돌연변이체의 배양 상등액의 pH는 각각 4.5와 8.5로 나타났다. 돌연변이체에서는 ampicillin, actinomycin D, bacitracin, lincomycin과 vancomycin 같은 항생제에 대한 저항성이 사라졌으며, 이러한 항생제에 대한 최소 억제 농도(MIC)가 급격하게 감소하였다. B-W 균주에서 분리한 플라스미드로 대장균을 형질전환시킨 결과, 원 균주와 같은 크기의 플라스미드가 이 형질전환 대장균에서 발견되었다. 플라스미드가 제거된 돌연변이체에서는 플라스미드가 발견되지 않았다. 20 kb 크기의 플라스미드에 2,3-DHB 생산 유전자와 여러 항생제 저항성 유전자가 자리잡고 있는 것으로 추정된다. Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at $43^{\circ}C$ was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and $MnSO_4$ containing medium at $28^{\circ}C$ for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli $DH5{\alpha}$ was transformed with plasmid isolated from strain B-W. The transformant E. coli $DH5{\alpha}$ harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli $DH5{\alpha}$ produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid.

한미FTA에 대한 소비자전망과 소비자태도

김경자,Kim, Kyung-Ja 한국가정관리학회 2009 가정과삶의질연구 Vol.27 No.6

This study examined the expectations and attitudes of urban Korean consumers with respect to the Korea-USA Free Trade Agreement (FTA), which was drafted on April, 2007. The data were collected through online surveys in which 527 adults answered structured questionnaires. The results showed that the number of consumers who favored the Korea-U.S.A. FTA was almost twice the number of those who objected to it. Most of the consumers surveyed expected not only positive effects but also negative ones from the FTA, and the more consumers expected positive effects from the FTA, the more they tended to have positive attitudes toward the trade agreement. The effects of consumers' individual characteristics on their expectations and attitudes were also analyzed in this experiment.