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대학병원 검사실직원의 감염 및 안전수칙준수에 대한 연구
김각현 ( Kag Hyun Kim ),송석규 ( Suk Gyu Soung ),유기원 ( Ki Won Yoo ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
This study evaluates determinants of compliance witli universal precautions in laboratory professionals. Universal precautions were first promulgated by the Centers for Disease Control(CDC) and subsequently formalized by the Occupational Safety and Health Administration in 1992. But, Still compliance with universal precautions appeared unsatisfied results. The reasons for low comp1iance include inadequate programs in safety management, employees`` perceptions that they cannot protect themselves while providing optimum patient care, and individual risk-taking tendencies. Generally, levels of aboratorians’ knowledge about universal precaution practices were 77.3%, and levels of aboratorians about personal protective equipment(PPE) compliance were 32.4%. Laboratory professionals who perceived their management to be more oriented to safety were 7.07 times more likely to comply with universal precautions governing PPE use. Laboratory professionals who perceived occupational risk of acquiring HIV were 5.30 times more likely to comply with universal precautions governing PPE use.
원저 : 혈액학검사 외부정도관리인 CAP Survey참여 경험
김각현 ( Kag Hyun Kim ),오진숙 ( Jin Sook Oh ),전희선 ( Hee Sun Jeon ),곽연식 ( Yun Sik Kwak ) 대한임상병리사협회 1997 임상혈액검사학회 발표자료집 Vol.4 No.1
The Department of Clinical Pathology, Ajou University Hospital has participated in CAP survey for the quality control of hematology and coagulation in 1995. External quality assessment is the objective evaluation by an outside agency of performance by a number of laboratories for standardization which is reduced specially on a regional or national basis. Three trials have been performed a year for many hematology tests, which are different at each trial. A commonly used evaluation criterion has been the comparison of proficiency test results with those of peer groups, considering all values that exceed ± 2 SD to be "unacceptable". CAP`s evaluation reports of our laboratory shown relatively satisfactory results except for fibrinogen. As the fibrinogen test produce various results, the stability of specimens provided to participants was an important consideration. From our experience, it was essential that specimens could be safely transported, without deterioration. Finally, the external quality assessment has contributed significantly towards an improvement in the quality of laboratory tests.
Bacteroides fragilis의 R-plasmid에 대한 분리와 전이
김각현 ( Kag Hyun Kim ),이경원 ( Kyung Won Lee ),장인호 ( In Ho Jang ),이형환 ( Hyung Hoan Lee ) 대한임상검사과학회 1990 대한임상검사과학회지(KJCLS) Vol.22 No.1
Bacteroides fragilis strains employed in this study were isolated from the W onju Christian Hospital patients by reference manual. A total 50 strains of B . fragilis isolated from clinical specimens were tested for the antimicrobial susceptibility, transmission of resistance and plasmid profiles. Plasmid profiles for molecular characterization of R- plasmid from B . fragilis strains were studied through the methods of alkaline SDS lysis and agarose gel electrophoresis. Antimicrobial resistant B. fragilis WBL · 360 isolate had 6 plasmid DNA elements, B. fragilis WBL 328, 195 had 5 plasmid DNA elements, WBL 107, 339 had 4 plasmid DNA elements, WBL 109 had 3 plasmid DNA elements, WBL 113 had 2 plasmid DNA elements but WBL 344 did not have any plasmid DNA elements. They had 3 to 7 different antibiotic resistance phenotypes, but WBL 344 strain had only one antibiotic resistance phenotype for rifampin. Plasmid transfer frequency of WBL 107 were Tetr: 2.4X10-7, Clnr: 2.5xl0-7 and ofWBL 360 were Tetr : 2. 3 X 10- 7 , Clnr : 7. 5 X 10- 7 ㆍ After matting, transconjugant WBL 107 strain confirm 7. 2 kb, 5. 6 kb plasmid band and transconingant WBL 360 strain confim 14.7 Kb plasmid band by gel electrophoresis.
김각현 ( Kag Hyun Kim ),이기연 ( Ki Youn Lee ),최정애 ( Jung Ae Choi ),신은숙 ( Eun Sook Shin ) 대한임상병리사협회 1996 임상수혈검사학회 발표자료집 Vol.3 No.1
To evaluate the current status of blood donation at the Ajou University Hospital. The authors investigated 1345 volunteered for blood donors from Jul. 1994. to Feb. 1996. Through the performing the physical examination history hemoglobin of blood and blood typing 1170 were found eligible for blood donation and 751 donated blood. The results are summarized as follows : 1. The age of donors mainly consisted or 20~29 group(48.5%), and 30~39 group(28.4%) with male to female ratio of 12.8 : 1 2. The occupation of donors was employee(35.9%), student(30.6%), public service personnel(60%), soldier(5.1%), and others(22.4%). 3. Distribution of blood groups were 38.2% in group A(+), 26.9% in group B(+), 28.5% in group O(+), 6.4 % in group AB(+). 4. Among the 283 whole blood donors, 34(12.0%) were rejected due to various diseases(38.2%), poor vessels(l4.7%), ABO incompatibility against the directed recipient(11.8%), underage donor(8.8%), and other causes. 5. Among the 1,062 pheresis donor bloods tested for serological markers. 141(13.3%) were rejected due to positive HBsAg(11.3%) elevated ALT level(47.5%), low hemoglobin(7.8%), positive anti-HCV(5.7%), reactive VDRL test(2.8%). 6. Departments that wanted the directed blood donors were mainly hematological Medicine. The rate of transfusion per a patient were 3.9 units.
신옥현 ( Ok Hyun Shin ),이은주 ( Eun Joo Lee ),김각현 ( Kag Hyun Kim ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2
Serum separation tubes(SST) conπibute to the reduction of laboratory errors and to the automation of testing processes. However, the glass tubes may be liable to breakage when they are inadvertently dropped on a hard surface or when the caps are opened. π11S increases the risk of puncture wounds and contamination of the blood. Recently, plastic blood-drawing tubes with potential advantage providing more safety to laboratoη employees have been introduced. We evaluated the usefulness of plastic evacuated blood-drawing tubes(SST 11 PLUS) in compared with the glass tubes in testing 26 chemistry analytes. There were no statistically significant differences between the analytes in the SST and in the SST 11 PLUS tubes or the period of storage in most assays. Some analytes including CO2, TG, AST, ALT, LDH showed a significant difference between the types of tubes. However, these differences were probably caused by the instability of the samples(e.g., CO2) or between-run variability and are not considered clinically significant. The values of K, Cl and CU2 also showed statistically significant differences by the storage time. However, K value in blood drawn in plastic tubes showed no significant difference for up to 5 days. In conclusion, the plastic SST 11 PLUS tubes can be substituted for the glass SST tubes in routine chemis따 analysis.