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권옥선,권수정,김진상,이건봉,맹한주,이정미,황귀서,차혁진,천광훈 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.3
Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiationinduced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ΔG ≥-34.6 kcal/mol, i-Score value ≥65, and siRNA scales score ≤30. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3’ overhang at the 3’ end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.
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In silico drug repositioning: from large-scale transcriptome data to therapeutics
권옥선,Wankyu Kim,Hyuk-Jin Cha,Haeseung Lee 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.10
Drug repositioning is an attractive alternative toconventional drug development when new beneficialeffects of old drugs are clinically validated because pharmacokineticand safety profiles are generally alreadyavailable. Since * 30% of drugs newly approved by theUS food and drug administration (FDA) are developedthrough drug repositioning, identifying novel usage forexisting drugs is an emerging strategy for developing diseasetreatments. With advances in next-generationsequencing technologies, available transcriptome datarelated to diseases have expanded rapidly. Harnessing theseresources enables a better understanding of disease mechanismsand drug mode of action (MOA), and moves towardpersonalized pharmacotherapy. In this review, we brieflyoutline publicly available large-scale transcriptome databasesand tools for drug repositioning. We also highlightrecent approaches leading to the discovery of novel drugtargets, drug response biomarkers, drug indications, anddrug MOA.
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5인 미만 사업장에서 산업보건에 관한 사업주와 근로자의 인식, 지식 및 태도
권옥선,정치경 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.3
In order to investigate the awareness, knowledge and attitude on occupational health in small industry with less than 5 workers, the self-administered questionnaire survey was carried out on 194 employers and 310 employees in 194 industries. The results were as follows : 1.The most frequent type of industry was manufacturing(98.5%) and the industry with 1-2 workers occupied 69.0%. 2.The employers and employees recognized noise and dust as the most harmful factors. 3.The awareness rate for preventing occupational disease in employers was the highest in nothing can be done and followed by improvement of work environment and consciousness and knowledge of workers on health. Those in employees was the highest in improvement of work environment and followed by consciousness and knowledge of workers on health and interest and investment of employer on health. 4.Supplied rate of the protective equipment were 41.7% in employers and 38.1% in employees. 14.4% in employers and 9.7% in employees always wore the protective equipment and the reasons for not wearing protective equipment were inconvenient(30.4%), bothering(22.4%), not necessary(14.9%), not supplied(13.5%) in employers and employees. 5.The significant factors influencing the knowledge and attitude on occupational health were age, education level and monthly income in the point of knowledge, and monthly income in the point of attitude. If knowledge were considered as a dependent variable, knowledge is significant factor for attitude on occupational health.
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Yangkyuksanhwa-Tang Attenuates Ischemic Brain Injury in a Focal Photothrombosis Stroke Model
Do-Kyung Han(한도경),Malk-Eun Pak(박맑은),Ok-Sun Kwon(권옥선),Byung-Tae Choi(최병태) 한국생명과학회 2019 생명과학회지 Vol.29 No.11
양격산화탕은 9가지의 약재로 구성된 처방으로 한의학적 뇌졸중 치료에 가장 널리 사용되는 처방 중 하나이며, 주로 사상체질이론의 소양인 뇌졸중 치료에 적용된다. 본 연구는 실험동물을 이용한 뇌졸중에 대한 양격산화탕의 효과에 대한 연구가 전무하여, photothrombosis로 유발된 허혈성 마우스모델을 이용하여 양격산화탕의 효과를 살펴 보았다. 동물행동학적 변화와 더불어 뇌손상에 미치는 영향을 뇌경색 용적에 대한 조직학적 검색 및 신경염증과 신생세포에 대한 면역조직화학적 검색으로 살펴 보았다. 동물행동학적 결과로 보아, 양격산화탕은 뇌허혈에 의해 손상된 운동기능, 즉 wire grip과 rotarod test에 의한 운동조정과 균형 능력 등에 대한 기능적 회복을 보였으며, 이는 조직학적 검색으로 관찰된 뇌경색 용적의 축소를 동반하였다. 면역조직화학적 결과를 보면, 양격산화탕은 tumor necrosis factor-α와 myeloperoxidase 면역반응세포의 수를 현저히 감소시켰다. 이와 반대로 양격산화탕은 glial fibrillary acidic protein와 ionized calcium-binding adapter molecule 1 면역반응세포의 수를 현저히 증가시켰다. 또한 양격산화탕은 Ki67/doublecortin 면역반응세포의 수를 현저히 증가시켰다. 이상의 결과로 보아, 양격산화탕은 항염증, astrocyte와 microglia의 활성화 및 신경세포의 증식을 통해 뇌경색 용적을 감소시키며, 이는 뇌허혈성 운동장애에 대한 완화 효과로 이어 지는 것을 알 수 있다. 따라서 양격산화탕은 뇌손상에 대한 신경기능적 완화효과를 보여 줌으로서 뇌졸중 환자에 대한 유효한 치료제로 사료된다. Yangkyuksanhwa-Tang (YKSH), consisting of nine different herbs, is commonly used in Soyangin-type individuals with stroke, based on the Sasang Constitution Theory in Korea. However, no evidence has yet confirmed a beneficial effect of YKSH in ischemic stroke treatment. In this study, we investigated the effects of YKSH on ischemic brain injury in a mouse model of cerebral ischemia. Focal cerebral ischemia in mice was induced by photothrombosis, and behavioral recovery was evaluated. Infarct volume, inflammation, and newly generated cells were evaluated by histology and immunochemistry. YKSH treatment resulted in a significant recovery from the motor impairments induced by focal cerebral ischemia, as determined with wire grip and rotarod tests. YKSH treatment also decreased the infarct volume and the number of cells positive for tumor necrosis factor-α and myeloperoxidase when compared with a vehicle-treated control group. By contrast, YKSH treatment considerably increased the number of cells positive for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1, as well as the number of cells doubly positive for Ki67/doublecortin when compared with the vehicle-treated group. These results suggest that YKSH treatment attenuated the infarct size by anti-inflammatory action, astrocyte and microglia activation, and neuronal proliferation, thereby facilitating neurofunctional recovery from a cerebral ischemic assault. YKSH could therefore be a potential treatment for neurofunctional restoration of the injured brains of patients with stroke.
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