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Calcium Carbonate-Mineralized Nanocarriers for Intracellular Delivery of Therapeutic Proteins
구안나,이홍재,이상천 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Anticancer protein drugs are superior to chemical drugs in terms of specificity, therapeutic efficacy, and safety. Hence, they are widely utilized for treatment of various diseases including cancers. However, due to poor cellular permeability and fragility of most proteins, intracellular delivery of proteins is challenging. In this work, we develop the calcium carbonate-based hybrid nanoparticles for efficient intracellular protein delivery. An apoptotic protein, cytochrome C (Cyt C) loaded nanoparticles were prepared by poly(ethylene glycol)-b-polyaspartate) (PEG-b-PAsp) mediated in-situ CaCO<sub>3</sub> mineralization in the presence of Cyt C. Cyt C-loaded calcium carbonate mineralized nanoparticles induced effective apoptosis of cancer cells.
구안나,임화평,박동진,김종호,정서영,이상천 한국고분자학회 2013 Macromolecular Research Vol.21 No.7
We report on a smart mesoporous silica nanoparticle (MSN) that can trigger the release of anti-inflammatory N-acetyl-L-cysteine (NAC) within the intracellular environment. NAC was conjugated to the pore surfaces of MSNs through glutathione (GSH)-cleavable disulfide linkages. Solid-state nuclear magnetic resonance (NMR),Fourier-transform infrared (FTIR) spectroscopy, and Brunauer-Emmett-Teller (BET) analyses confirmed the successful NAC conjugation to the pore walls. The release of NAC from the NAC-conjugated MSN (MSN-NAC) could be controlled by adjusting the concentration of GSH regarding the release media. At an extracellular level of GSH (10 μM), the NAC release was greatly inhibited, whereas, at an intracellular level of GSH (2 mM), MSN-NAC facilitated the release of NAC. Confocal laser scanning microscopy (CLSM) studies showed that the NAC release was effectively triggered by intracellular GSH after uptake by BV-2 microglial cells. The MSN developed in this work may serve as the efficient intracellular carriers of NAC for the treatment of neuroinflammation.
구안나,권일근,이상천,이순기,김형섭,우이형,정성형,채지화,강계원 한국고분자학회 2010 Macromolecular Research Vol.18 No.10
Novel porous poly(L-lactide) (PLLA) scaffolds with surface-immobilized nano-hydroxyapatite (N-HAp)were synthesized for effective bone regeneration. N-Hap-bearing surface phosphate functionalities were immobilized chemically on the pore surface of amine-treated PLLA scaffolds. Thermogravimetric analysis (TGA) showed that 24 wt% of N-HAp had been immobilized on the scaffold pore surface. Field-emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) confirmed that N-HAp was exposed markedly on the pore surfaces of scaffolds compared to the conventional scaffold fabricated by a bulk-mixing process of PLLA and N-HAp. The PLLA scaffold with surface-immobilized N-HAp had a higher surface Ca atomic ratio (4.6%) than that on the pore surface of the bulk-mixed PLLA/N-HAp scaffold (Ca = 0.4%). The porous PLLA scaffolds with surfaceimmobilized N-HAp provided favorable environments for enhanced in vivo bone tissue growth, which was estimated by hematoxylin and eosin (H&E) staining for organic bone tissues and alizarin red S staining for inorganic bone minerals. These results suggest that the N-HAp immobilization approach may produce many useful scaffolds with pore surfaces featuring excellent bone tissue-regenerative properties.
아산화질소(N2O)와 공기(Air)를 사용한 마취 수술의 기낭압 조정이 수술 후 인후통과 쉰목소리에 미치는 영향
구안나,유미 한국간호과학회 2019 Journal of Korean Academy of Nursing Vol.49 No.2
Purpose: To investigate the differences in postoperative sore throat and hoarseness by adjustment of endotracheal tube cuff pressure (CP) during nitrous oxide (N2O) and air anesthesia. Methods: A one-equivalent control group pretest-posttest design was used. Data were collected from August 8 to October 19, 2017 and analyzed using the independent t-test and repeated measures ANOVA. Eighty-four participants were enrolled and divided into three groups: 28 in the Control Group (CP adjusted every 30 minutes using N2O), 28 in Experimental Group 1 (CP adjusted every 10 minutes using N2O), and 28 in Experimental Group 2 (non-adjusted CP using air), all of whom underwent urologic, gynecologic, and orthopedic surgeries at the G University hospital. Sore throat was assessed using a numeric rating scale; hoarseness was evaluate using the Stout classification at 1, 6, and 24 hours after surgery. Results: Scores for sore throat and hoarseness were significantly different between the groups at each measurement time, and scores were consistently higher in the control group. During subsequent measurements, sore throat and hoarseness scores were significantly lower at 6 hours. Cuff pressure changed significantly using air anesthesia (c2=10.41, p=.015) up to 2 hours after induction. Severe sore throat and hoarseness was observed for up to 6 hours after surgery. Conclusion: Cuff pressure adjustment at short time intervals would be helpful in reducing postoperative sore throat and hoarseness. Nursing intervention focused on prevention of sore throat and hoarseness should be required up to 6 hours postoperatively in patients undergoing endotracheal intubation.