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Leptospira canicola 실험적 감염햄스타의 조직절편내 면역조직화학적 균체항원검출
구복경,김순복,서정향,문윤경,정석찬,박정문,박용호,문진산,Ku, Bok-gyeong,Kim, Soon-bok,Sur, Jung-hang,Moon, Oun-kyong,Jung, Suk-chan,Park, Jung-moon,Park, Yong-ho,Mun, Jin-san 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
The present study was done to detect Leptospira canicola antigens in paraffin sections from experimentally infected hamsters with PAP method. The anti-Leptospira canicola serum used as first antibody was prepared by immunizing rabbits with Leptospira canicola. Positive reactions were detected in interstitium, tubular epithelial cells and glomerulus of the kidney, and in the hepatic sinusoid. With PAP method, leptospiral antigens were detected at the 48 hours after bacterial infection, but isolation, silver stain, and dark field microscopic examination of the samples did 48 hours later than PAP method. The results suggested that PAP method is considered as a reliable tool for confirmative diagnosis of this disease.
돼지 단독균 인공감염 및 항생제 치료에 따른 Haptoglobin치의 변화
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Joang-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The changes of concentration of serum haptoglobin, body temperature, hematological result and antibody titers were compared in pigs given intravenously injection of E rhusiopathiae and control pigs. Pigs administered with E rhusiopathiae injections showed acute septicemia and arthritis. Also, statistically significant changes observed in the total white cell counts and body temperature. An increased haptoglobin concentration in serum was detected after E rhusiopathiae infection, but not during antibiotics treatment. The results indicated that measurement of the concentration of serum haptoglobin may be useful in the early diagnosis of infectious disease, such as Erysipelas.
문진산,주이석,구복경,김종염,김덕원,박용호,한태욱,Moon, Jin-san,Joo, Yi-seok,Ku, Bok-gyeong,Kim, Jong-yeom,Kim, Duck-won,Park, Yong-ho,Hahn, Tae-wook 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
The effects of chitosan on mastitis in lactating holstein cows were evaluated. Fifty six cows with intramammary infection(IMI) from nine farms were selected and the cows were fed with diets which contained 15~20g chitosan per day for 5~7 days. The milk samples were obtained from cows at 7 days and 14 days after administration to determine effect of the curing of mastitis and the reduction of somatic cell counts(SCC). The average value of SCC levels in quarter milk from the cows administrated with chitosan significantly decreased up to 31.8% and 47.7% at 7 and 14 days, respectively(P<0.05). The cure rates of chitosan for Stapylococcus aureus, coagulase-negative Staphylococci, Streptococci spp, other gram positive bacteria and coliforms were 30.4, 42.8, 33.3, 66.6 and 54.5 % respectively. Twenty three out of 64 cases were cured by feeding with chitosan. The results showed that administration of chitosan could reduce SCC in milk and improve cure rates of bovine mastitis caused by microorganisms. The further studies will be pursued to study on the mechanism of chitosan in the immune responses of cows with mastitis.
돼지에서 감염의 조기진단 지표로서의 Haptoglobin 적용에 관한 연구
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Jong-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
Sera of pigs with clinically normal and infectious conditions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) to demonstrate the specific changes in protein profile. In the sera from pigs with infection, haptoglobin with a 40KDa protein was found to be increased as compared to that of sera from normal pigs. As a rapid detection method for monitoring infections at large-scale farms, one of acute phase protein, haptoglobin, was selected to compare the concentrations between infectious and non-infectious conditions. Haptoglobin concentrations were low in pigs with clinically normal conditions but significantly increased in pigs with Aujesky's disease, hog cholera and parvo-virus infection. The studies provide that haptoglobin can be used as an indicator to monitor infections early at farm level.
백혈구 표면항원 특이 단크론항체를 이용한 한우의 말초혈액 백혈구 아군에 관한 연구
문진산,박용호,정석찬,구복경,강병규,Moon, Jin-san,Park, Yong-ho,Jung, Suk-chan,Ku, Bok-gyeong,Kang, Byong-kyu 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.
돼지 오제스키바이러스 재조합단백질 gp50, gIII와 α-ADV을 이용한 Quil A-ISCOMs 접종시 백혈구아군 분포율에 관한 연구
문진산,박용호,정석찬,구복경,이성일,현방훈,안수환,Moon, Jin-san,Park, Yong-ho,Jung, Suk-chan,Ku, Bok-gyeong,Lee, Sung-il,Hyun, Bang-hun,An, Soo-hwan,Davis, W.C. 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
An effective candidate subunit vaccine was prepared by using the immunostimulating complexs(ISCOMs) with Quil A and recombinant protein(gp50, gIII and inactive $\alpha$-ADV) Aujeszky's disease virus(ADV). The weaned pigs were twice immunized with a ADV-ISCOMs, and followed by intramuscular challenge with $1{\times}10^4$ $TCID_{50}$ ADV(strain Yangsan). The unvaccinated pigs were also challenged with same dose of ADV. At 5 days after challenge, the control pigs have developed ADV clinical signs. Whereas, the vaccinated pigs protected them from ADV-induced acute symptoms and death. Also, to identify the lymphocyte subpopulation in peripheral blood with pigs from ADV-ISCOMs vaccinated and control group, lymphocyte reacted with a panel of monoclonal antibodies which are specific to swine leukocyte surface antigens and assayed by the flow cytometry. MHC class I, CD2, CD8, N cells, CD11a, and CD45 antigen positive cells were decreased after inoculating virulent ADV Yangsan strain in control group. The data indicated that ISCOMs technique was useful in ADV subunit vaccine preparation and demonstrated the importance of gp50, gIII as a component of ADV vaccine.